Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1223,626,1060TG47.1% 20.7 / 12.5 34V174G (GTG→GGG) hcf136_3Ycf48‑like protein
Reads supporting (aligned to +/- strand):  ref base T (16/2);  new base G (2/14);  total (18/16)
Fisher's exact test for biased strand distribution p-value = 1.45e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

GCAGCCGAGGCTGCCGCAGACGAGCGTCGCCTGGCGGCGGCGCAACGGCTGCTGGCCGACGGCGCGGACAAGCCGTTGTTGGCCCTGAATTTCACCGATGCCCGCAACGGCCTGGTCGTCGGTGCCTACGGGGTGGCCATGCGCACC‑GACGACGGCGGGCGCAGCTGGCAGTCGGAGATCGGCCGACTGCCGAACGGGCGTGGACTGCACCTGTATGCCCTGGCGCGCCAAGGCAACCAGGTGTACATCGCCGGCGAGCAGG  >  ACB122/3625973‑3626234
                                                                                                                                     |                                                                                                                                 
gcagCCGAGGCTGCCGCAGACGAGCGTCGCCTGGCGGCGGCGCAACGGCTGCTGGCCGACGGCGCGGACAAGCCGTTGTTGGCCCTGAATTTCACCGATGCCCGCAACGGCCTGGTCGTCGGTGCCGACGGGGTg                                                                                                                                  >  1:300772/1‑135 (MQ=255)
     cGAGGCTGCCGCAGACGAGCGTCGCCTGGCGGCGGCGCAACGGCTGCTGGCCGACGGCGCGGACAAGCCGTTGTTGGCCCTGAATTTCACCGATGCCCGCAACGGCCTGGTCGTCGGTGCCTACGGGGTGGCCAt                                                                                                                             >  2:19358/1‑135 (MQ=255)
          cTGCCGCAGACGAGCGTCGCCTGGCGGCGGCGCAACGGCTGCTGGCCGCCGGCGCGGACAAGCCGTTGTTGGCCCTGAATTTCACCGATGCCCGCAACGGCCTGGTGGTCGGTGCCGACGGGGGGGCCATgcgcc                                                                                                                        >  1:184705/1‑134 (MQ=255)
            gCCGCAGACGAGCGTCGCCTGGCGGCGGCGCAACGGCTGCTGGCCGACGGCGCGGACAAGCCGTTGTTGGCCCTGAATTTCACCGATGCCCGCCACGGCCTGGTCGTCGGTGCCTACGGGGTGGCCAAGCGCAcc                                                                                                                      >  6:532639/1‑135 (MQ=255)
                             ccTGGCGGCGGCGCAACGGCTGCTGGCCGACGGCGCGGACAAGCCGTTGTTGGCCCAGAATTTCACCGATGCCCGCAACGGCCTGGTCGGCGGTGCCTACGGGGTGGCCAATCGCCCC‑GCCCACGGCGGgcgccg                                                                                                    >  8:5403/1‑133 (MQ=255)
                                ggcggcggcGCAACGGCTGCTGGCCGACGGCGCGGACAAGCCGTTGTTGGCCCTGCATTTCACCGCTGCCCGCCACGGCCTGGTGGTGGGGGGCGTCCGGGTGGCCAATCGCCCC‑CCCGCCGGCCGGcgccgcgg                                                                                                 >  5:393254/1‑133 (MQ=255)
                                          cAACGGCTGCTGGCCGACGGCGCGGACAAGCCGTTGTTGGCCCTGAATTTCACCGATGCCCGCAACGGCCTGGTCGTCGGTGCCTACGGGGTGGCCATGCGCACC‑GACGACGGCGGGCGCAGCTGGCAGTc                                                                                           >  3:160505/1‑131 (MQ=255)
                                           aaCGGCTGCTGGCCGCCGGCGCGGACAAGCCGTTGTTGGCCCTGAATTTCACCGATGCCCGCAACGGCCTGGTCGTGGGGGCCTACGGGGTGGCCCTGCGCACC‑GCCGCCGGCGGGCGCCGCGGGCAGGCggggc                                                                                      >  4:448011/1‑132 (MQ=255)
                                           aaCGGCTGCTGGCCGACGGCGCGGACAAGCCGTTGTTGGCCCTGAATTTCACCGATGCCCGCAACGGCCTGGTCGTGGGTGCCGACGGGGTGGGCCTGCGCCCC‑GCCGCCGGCGGGCGCCGCTGGCCGTCGgag                                                                                       >  7:234344/1‑134 (MQ=255)
                                                ctgctgGCCGACGGCGCGGACAAGCCGTTGTTGGCCCTGAATTTCACCGATGCCCGCAACGGCCTGGTCGTCGGTGCCTACGGGGTGGCCATGCGCACC‑GACGACGGCGGGCGCAGCTGGCAGTCGGAGATCGGc                                                                                 >  4:392876/1‑135 (MQ=255)
                                                        ggcgggCGCGGACACGCCGTTGTGGGCCCTGAATTCCACCCAGGCCCGCAACGGCCTGGGCGCCGGTGCCTACGGGGGGGCCATGCCCACC‑GACGACGGCGGGCGCAGCTGGCAGTCGGAGATCGGCCGACTGcc                                                                         <  6:233983/131‑1 (MQ=255)
                                                        cGAGGGCGCGTACACGCGGTTGTGGCCCCTGAATCTACCCGAGGCCGCCCCCGGCCTGGGCGTCGGTGCCTACGGGGTGGCCTTGCCCCCC‑GACGACGGCGGGCGCAGCTGGCAGTCGGAGATCGGCCGACTGcc                                                                         <  2:405605/135‑1 (MQ=255)
                                                             gcgcGGACACGCCGTTGTTGCCCCTGAATTTCCCCCAGGCCCGCACGGGCCGGGGCGGGGGTGCCTCCGGGGGGCCAATGCGCACC‑GACGACGGCGGGCGCAGCTGGCAGTCGGAGATCGGCCGACTGCCGAACg                                                                    <  7:236718/135‑1 (MQ=255)
                                                                cgGACACGCGGTTGTGGGCCCTCAATTCCCCCGAGGCCCCCACCGGCGGGGGGGGCGGGGCCTCGGGGGGGCCCAGGCGCACC‑GACGACGGCGGGCGCAGCTGGCAGTCGGAGATCGGCCGACTGCCGAACGGGc                                                                 <  7:343360/135‑1 (MQ=255)
                                                                  gACACGCCGTTGTTGGCCCTTAATTACCCCGAGCCTCGCACGGGCCTGGGCGTCGGGGCCTACGGGGGGGCCAGGCGCACC‑GACGACGGCGGGCGCAGCTGGCAGTCGGAGATCGGCCGACTGCCGAACGGGCGt                                                               <  8:182765/135‑1 (MQ=255)
                                                                  gACAAGCCGTTGTGGGCCCGGAATTTCACCGATGCCCCCACCGGCCTGGGCGTGGGTGCCTACGGGGGGGCCATGGGCCCC‑GACGACGGCGGGCGCAGCTGGCAGTCGGAGATCGGCCGACTGCCGAACGGGCGt                                                               <  8:289683/135‑1 (MQ=255)
                                                                   accaGCCGTTGTTGGCCCTGAATTTCACCGATGCCCGCAACGGCCTGGTCGTAGGTGCCTACGGGGTGGCCCTGCGCACC‑GACGACGGCGGGCGCCGCTGGCAGTCGGAGATCGGCCGACTGCCGAACGGGCGTg                                                              >  5:257477/4‑135 (MQ=255)
                                                                   aCAAGCCGTTGTTGGCCCTGAATTTCACCGATGCCCGCAACGGCCTGGTCGTCGGTGCCGACGGGGTGGCCATGCGCACC‑GACGCCGGCGGGCGCAGCTGGCAGTCGGAGATCGGCCGACTGCCGAACGGGCGTg                                                              >  4:157957/1‑135 (MQ=255)
                                                                     aaGCGGTTGTTGGCCCTGAATTACCCCTCGGCCCCCACCGGCGGGGGGTGCGGGGCTTCGGGGGGGGCCATGCGCCCC‑GACGACGGCGGGCGCAGCTGGCAGTCGGAGATCGGCCGACTGCCGAACGGGCGTGGa                                                            <  3:311310/135‑1 (MQ=255)
                                                                       gCCGTTGTTGGCCCTCAATTTCACCGAGGCCCGCAACGGCCTGGGCTTCGGGGCCTACGGGGGGGCCAGGCGCCCC‑GACGACGGCGGGCGCAGCTGGCAGTCGGAGATCGGCCGACTGCCGAACGGGCGTGGACt                                                          <  7:159760/135‑1 (MQ=255)
                                                                        ccGTTGTTGGCCCTGAATTCCCCCGATCCCCGCACCGGCGGGGGCGGGGGGGCCTCCGGGGGGGCCAGGCCCACC‑GAGGACGGCGGGCGCAGCTGGCAGTCGGAGATCGGCCGACTGCCGAACGGGCGTGGACTg                                                         <  4:491675/135‑1 (MQ=255)
                                                                          gttgttGGCCCTGAATTTCACCGATGCCCGCAACGGCCTGGTCGTAGGTGCCTACGGGGTGGCCATGCGCCCC‑GACGACGGCGGGCGCAGCTGGCAGTCGGAGATCGGCCGACTTCCGCACGGGCGTGGACTTCa                                                       >  1:9194/1‑135 (MQ=255)
                                                                            tgttgGCCCTGAATTTCACCGATGCCCGCACCGGCCTGGGCGTCGGTGCCTACGGGGGGGCCAGGCGCACC‑GCCGACGGCGGGCGCAGCTGGCAGTCGGAGATCGGCCGACTGCCGAACGGGCGTGGACTGCAcc                                                     <  3:392876/135‑1 (MQ=255)
                                                                                ggCCCTGAATTTCACCGATGCCCGCAACGGCCTGGTCGTCGGTGCCTACGGGGTGGCCATTCGCACC‑GACGACGGCGGGCGCAGCTGGCAGTCGGAGATCGGCCGCCTGCCGAACGGGCGTGGACTGCACCAGTa                                                 >  1:55614/1‑135 (MQ=255)
                                                                                    cTGAATTTCACCGATGCCCGCAACGGCCAGGTACTGCGTGCCGACGGGGGTGCCATGCGCAAC‑GCCCACGGCGCGCGCCGCTGGCAGTCGGGGATCCGCCGACAGCCCAAAGGGCCGGGGCTGCACCAGTATGcc                                             >  6:369664/1‑135 (MQ=255)
                                                                                        aTTTCACCGATGCCCGCAACGGCCTGGTCCTCGGTGCCAACGGGGTGGCCATGCGCCCC‑GACGAAGGCCGGCGCAGCAGGCAGTCCGAGATCGGGCGACTGCCGCACGGGCGTGGACATCACCTGGATGCCCAgg                                         >  2:409751/1‑135 (MQ=255)
                                                                                                 aTGCCCGCACCGGCCGGGGCGTCGGTGCCTACGGGGTGGCCATGCGCACC‑GACGACGGCGGGCGCAGCTGGCAGTCGGAGATCGGCCGACTGCCGAACGGGCGTGGACTGCACCTGTATGCCCTGGCGCGCCAAg                                <  4:145245/135‑1 (MQ=255)
                                                                                                   gCCCGCACCGGCCTGGGCGGCGGGGCCTCCGGGGGGCCCATGCGCCCC‑GACGACGGCGGGCGCAGCTGGCAGTCGGAGATCGGCCGACTGCCGAACGGGCGTGGACTGCACCTGTATGCCCTGGCGCGCCAAGGc                              <  6:247689/135‑1 (MQ=255)
                                                                                                   ccccGCACCGGCGGGGGCGGGGGGGCCTCGGGGGGGGCCAGGCGCCCC‑GACGACGGCGGGCGCAGCTGGCAGTCGGAGATCGGCCGACTGCCGAACGGGCGTGGACTGCACCTGTATGCCCTGGCGCGCCAAGGc                              <  8:325252/134‑1 (MQ=255)
                                                                                                      cgcacgggCGTGGTGGTGGGTGCCT‑CGGGGGGGCCATTCGCCCCGGACGACGGCGGGCGCAGCTGGCAGTCGGAGATCGGCCGACTGCCGAACGTGCGTGGACTGCACCTGTATGCCCTGGCGCGCCAAGGCAAc                           <  2:188930/129‑1 (MQ=255)
                                                                                                                    cggcgGGGCCTCGGGGGGGGCCAGCGGCACG‑GCCGACGGCGGGCGCAGCTGGCAGTCGGAGATCGGCCGACTGCCGAACGGGCGTGGACTGCACCTGTATGCCCTGGCGCGCCAAGGCAACCAGGTGTACATCGc             <  4:199697/132‑1 (MQ=255)
                                                                                                                          gcccTGCGGGGGGGCCCGGCCCCCC‑CACGACGGCGGGCGCAGCTGGCAGTCGGAGATCGGCCGACTGCCGAACGGGCGTGGACTGCACCTGTATGCCCTGGCGCGCCAAGGCAACCAGGTGTACATCGCCGGCGa       <  2:125661/133‑1 (MQ=255)
                                                                                                                             cTACGGGGTGGCCATGCGCACC‑GACGACGGCGGGCGCAGCTGGCAGTCGGAGATCGGCCGACTGCCGAACGGGCGTGGACTGCCCCTGTATGCCCTGGCGCGCCAAGGCCACCAGGTGTACATCGCCCGCGCGCa    >  6:184923/1‑135 (MQ=255)
                                                                                                                               aCGGGGTGGCCATGCGCACC‑GACGACGGCGGGCGCAGCTGGCAGTCGGAGATCGGCCGACTGCCGAACGGGCGTGGACTGCACCTGTTTGCCCTGGCGCGCCAAGGCAACCAGGTGTACATCGCCGGCGAGCAgg  >  3:129915/1‑135 (MQ=255)
                                                                                                                                     |                                                                                                                                 
GCAGCCGAGGCTGCCGCAGACGAGCGTCGCCTGGCGGCGGCGCAACGGCTGCTGGCCGACGGCGCGGACAAGCCGTTGTTGGCCCTGAATTTCACCGATGCCCGCAACGGCCTGGTCGTCGGTGCCTACGGGGTGGCCATGCGCACC‑GACGACGGCGGGCGCAGCTGGCAGTCGGAGATCGGCCGACTGCCGAACGGGCGTGGACTGCACCTGTATGCCCTGGCGCGCCAAGGCAACCAGGTGTACATCGCCGGCGAGCAGG  >  ACB122/3625973‑3626234

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑