| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | ACB122 | 3,065,508 | G→A | V53M (GTG→ATG) | rcsC_6 → | Sensor histidine kinase RcsC |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | ACB122 | 3,065,508 | 0 | G | A | 98.4% | 215.6 / NA | 64 | V53M (GTG→ATG) | rcsC_6 | Sensor histidine kinase RcsC |
| Reads supporting (aligned to +/- strand): ref base G (0/0); major base A (26/37); minor base C (1/0); total (27/37) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.22e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GGGCGTTGGCCGGGCTGCTGGGGCTGGGTGACCACTCGGCGCGTAAAAGCCATTACCCGGAGCTGTCCGCCCGCCTGGACGAACTGGAAGCCGAACGCAACCGTTACAAATGGCTGTTCGAAAACGCCGTGCACGGTATTTTCCAGGCCAGCCTTCAGGACGGCATGCGTGCCGCCAACCCGGCGCTGGCGCGCATGCTCGGCTATGACGACCCGCAAGCGGTGCTGTTTTCCCTGACTGACCTGGCCGCCAACCTGTT > ACB122/3065380‑3065638 | gggCGGTGGCCGGGCTGCTGGGGCTGGGTGACCACTCGGCGCGTAAAAGCCATTACCCGGAGCTGTCCGCCCGCCTGGACGAACTGGAAGCCGAACGCAACCGTTACAAATGGCTGTTCGAAAACGCCATGCACg < 7:439104/135‑1 (MQ=255) gCCGGGCTGCTGGGGCTGGGTGACCACTCGGCGCGTAAAAGCCATTACCCGGAGCTGTCCGCCCGCCTGGACGAACTGGAAGCCGAACGCAACCGTTACAAATGGCTGTTCGAAAACGCCATGCACGGTATTTTc < 8:112235/135‑1 (MQ=255) cGGGCTGCTGGGGCTGGGTGACCACTCGGCGCGTAAAAGCCATTACCCGGAGCTGTCCGCCCGCCTGGACGAACTGGAAGCCGAACGCAACCGTTACAAATGGCTGTTCGAAAACGCCATGCACTGTATTTTCCa < 5:190316/135‑1 (MQ=255) ggCTGCTGGGGCTGGGTGACCACTCGGCGCGTAAAAGCCATTACCCGGAGCTGTCCGCCCGCCTGGACGAACTGGAAGCCGAACGCAACCGTTACAAATGGCTGTTCGAAAACGCCATGCACGGTATTTTCCAgg < 4:230169/135‑1 (MQ=255) ggCTGCTGGGGCTGGGTGACCACTCGGCGCGTAAAAGCCATTACCCGGAGCTGTCCGCCCGCCTGGACGAACTGGAAGCCGAACGCAACCGTTACAAATGGCTGTTCGAAAACGCCATGCACGGTATTTTCCAgg < 6:426657/135‑1 (MQ=255) tgctgGGGCTGGGTGACCACTCGGCGCGTAAAAGCCAATACCCGGAGCTGTCCGCCCGCCTGGACGAACTAGAAGCCGACCGCAACCGTTACAAATGGCTGTTCGAAAACGCCAAGCACGGTATTTTCCAGGccc > 4:351766/1‑134 (MQ=255) gctggggctgggTGACCACTCGGCGCGTAAAAGCCATTACCCGGAGCTGTCCGCCCGCCTGGACGAACTGGAAGCCGCACGCAACCGTTACAAATGGCTGTTCGAAAACGCCCTGCACGGTATTTTCCAGGcccg > 3:19161/1‑133 (MQ=255) gctggggctgggTGACCACTCGGCGCGTAAAAGCCATTACCCGGAGCTGTCCGCCCGCCTGGACGAACTGGAAGCCGAACGCAACCGTTACAAATGGCTGTTCGAAAACGCCATGCACGGTATTTTCCAGGCCAg > 1:369048/1‑135 (MQ=255) ctggggcggggGGACCACTCGGCGCGTAAAAGCCATTACCCGGAGCTGTCCGCCCGCCTGGACGAACTGGAAGCCGAACGCAACCGTTACAAATGGCTGGTCGAAAACGCCATGCACGGTATTTTCCAGGCCAGc < 8:445271/135‑1 (MQ=255) tggggctgggTGACCACTCGGCGCGTAAAAGCCATTACCCGGAGCTGTCCGCCCGCCTGGACGAACTGGAAGCCGAACGCAACCGTTACAAATGGCTGTTCGAAAACGCCATGCACGGTATTTTCCAGGCCAGcc < 8:341560/135‑1 (MQ=255) ggctgggTGACCACTCGGCGCGTAAAAGCCATTACCCGGAGCTGTCCGCCCGCCTGGACGAACTGGAAGCCGAAAGCAACCGTTACAAATGGCTGTTCGAAAACGCCATGCACGGTATTTTCCAGGCCAGCCTTc > 5:221712/1‑135 (MQ=255) gggTGACCACTCGGCGCGTAAAAGCCATTACCCGGAGCTGTCCGCCCGCCTGGACGAACTGGAAGCCGAACGCAACCGTTACAAATGGCTGTTCGAAAACGCCATGCACGGTATTTTCCAGGCCAGCCTTCCGGa > 2:397337/1‑135 (MQ=255) gggTGACCACTCGGCGCGTAAAAGCCATTACCCGGAGCTGTCCGCCCGCCTGGACGAACTGGAAGCCGAACGCAACCGTTACAAATGGCTGTTCGAAAACGCCATGCACGGTATTTTCCAGGCCAGCCTTCAGGa > 1:291598/1‑135 (MQ=255) ggTGACCACTCGGCGCGTAAAAGCCATTACCCGGAGCTGTCCGCCCGCCTGGACGAACTGGAAGCCGAACGCAACCGTTACAAATGGCTGTTCGAAAACGCCATGCACGGTATTTTCCAGGCCAGCCTTCAGGAc < 8:30256/135‑1 (MQ=255) ggTGACCACTCGGCGCGTAAAAGCCATTACCCGGAGCTGTCCGCCCGCCTGGACGAACTGGAAGCCGAACGCAACCGTTACAAATGGCTGTTCGAAAACGCCATGCACGGTATTTTCCAGGCCAGCCTTCAGGAc > 5:397481/1‑135 (MQ=255) cccGGAGCTGTCCGCCCGCCTGGACGAACTGGAAGCCGAACGCAACCGTTACAAATGGCTGTTCGAAAACGCCATGCACGGTATTTTCCAGGCCAGCCTTCAg < 8:187023/103‑1 (MQ=255) cccGGAGCTGTCCGCCCGCCTGGACGAACTGGAAGCCGAACGCAACCGTTACAAATGGCTGTTCGAAAACGCCATGCACGGTATTTTCCAGGCCAGCCTTCAg > 7:187023/1‑103 (MQ=255) cccGGAGCTGTCCGCCCGCCGGGACGAACTGGAAGCCGAACGCAACCGTTACAAATGGCTGTTCGAAAACGCCATGCACGGTATTTTCCAGGCCAGCCTTCAGGACGGCATGCGGGCCGCCAACCCGGCGCTGgc < 8:277372/135‑1 (MQ=255) ccGGAGCTGTCCGCCCGCCTGGACGAACTGGAAGCCGAACGCAACCGTTACAAATGGCTGTTCGAAAACGCCATGCACGGTATTTTCCAGGCCAGCCTTCAGGACGGCATGCGGGCCGCCAACCCGGCGCTGgc < 8:482505/134‑1 (MQ=255) gCTGTCCGCCCGCCTGGACGAACTGGAAGCCGAACGCAACCGTTACAAATGGCTGTTCGAAAACGCCATGCACGGTATTTTCCAGGCCAGCCTTCAGGACGGCATGCGTGCCGCCAACCCGGCGCTGGCGCGCAt < 6:191941/135‑1 (MQ=255) gCTGTCCGCCCGCCTGGACGAACTGGAAGCCGAACGCAACCGTTACAAATGGCTGTTCGAAAACGCCATGCACGGTATTTTCCAGGCCAGCCTTCAGGACGGCATGCGTGCCGCCAACCCGGCGCTGGCGCGCAt < 6:505973/135‑1 (MQ=255) gCTGTCCGCCCGCCTGGACGAACTGGAAGCCGAACGCAACCGTTACAAATGGCTGTTCGAAAACGCCATGCACGGTATTTTCCAGGCCAGCCTTCAGGACGGCATGCGTGCCGCCAACCCGGCGCTGGCGCGCAt < 2:369048/135‑1 (MQ=255) gCTGTCCGCCCGCCTGGACGAACTGGAAGCCGAACGCAACCGTTACAAATGGCTGTTCGAAAACGCCATGCACGGTATTTTCCAGGCCAGCCTTCAGGACGGCATGCGTGCCGCCAACCCGGCGCTGGCGCGCAt < 2:220126/135‑1 (MQ=255) tGTCCGCCCGCCTGGACGAACTGGAAGCCGAACGCAACCGTTACAAATGGCTGTTCGAAAACGCCATGCACGGTATTTTCCAGGCCAGCCTTCAGGACGGCATGCGTGCCGCCAACCCGGCGCTGGCGCGCATGc > 3:33906/1‑135 (MQ=255) tGTCCGCCCGCCTGGACGAACTGGAAGCCGAACGCAACCGTTACAAATGGCTGTTCGAAAACGCCATGCACGGTATTTTCCAGGCCAGCCTTCAGGACGGCATGCGTGCCGCCAACCCGGCGCTGGCGCGCATGc > 6:455581/1‑135 (MQ=255) tGTCCGCCCGCCTGGACGAACTGGAAGCCGAACGCAACCGTTACAAATGGCTGTTCGAAAACGCCATGCACGGTATTTTCCAGGCCAGCCTTCAGGACGGCATGCGTGCCGCCAACCCGGCGCTGGCGCGCATGc > 5:542942/1‑135 (MQ=255) tGTCCGCCCGCCTGGACGAACTGGAAGCCGAACGCAACCGTTACAAATGGCTGTTCGAAAACGCCATGCACGGTATTTTCCAGGCCAGCCTTCAGGACGGCATGCGTGCCGCCAACCCGGCGCTGGCGCGCATGc > 4:186332/1‑135 (MQ=255) ggccgcccgcGTGGACGAAGTGGAAGCCGAACGCAACCGTTACAAATGGCTGTTCGAAAACGCCATGCACGGTATTTTCCAGGCCAGCCTTCAGGACGGCATGCGTGCCGCCAACCCGGCGCTGGCGCGCATGCt < 8:340792/133‑1 (MQ=255) gcccgccTGGACGAACTGGAAGCCGAACGCAACCGTTACAAATGGCTGTTCGAACACGCCATGCACGGTATTTTCCAGGCCAGCCTTCAGGACGGCATGCGTGCCGCCAACCCGGCGCGGGCGCGCATGCTCgg > 5:339035/1‑134 (MQ=255) gcccgccTGGACGAACTGGAAGCCGAACGCAACCGTTACAAATGGCTGTTCGAAAACGCCATGCCCGGTATTTTCCAGGCCAGCCTTCAGGCCGGCATGCGTGCCGCCCGCCCGGCGCTGGCGCGCCTGCTCGGc > 6:55752/1‑135 (MQ=255) gcccgccTGGACGAACTGGAAGCCGAACGCAACCGTTACAAATGGCTGTTCGAAAACGCCATGCACGGTATTTTCCAgg > 7:423652/1‑79 (MQ=255) gcccgccTGGACGAACTGGAAGCCGAACGCAACCGTTACAAATGGCTGTTCGAAAACGCCATGCACGGTATTTTCCAgg < 8:423652/79‑1 (MQ=255) gcccgccTGGACGAACTGGAAGCCGAACGCAACCGTTACAAATGGCTGTTCGAAAACGCCATGCACGGTATTTTCCAGGCCAGCCTTCAGGACGGCATGCGTGCCGCCAACCCGGCGCTGGCGCGCATGCTCgg > 4:33053/1‑134 (MQ=255) gcccgccTGGACGAACTGGAAGCCGAACGCAACCGTTACAAATGGCTGTTCGAAAACGCCATGCACGGTATTTTCCAGGCCAGCCTTCAGGACGGCATGCGTGCCGCCAACCCGGCGCTGGCGCGCATGCTCgg > 2:435998/1‑134 (MQ=255) gcccgccTGGACGAACTGGAAGCCGAACGCAACCGTTACAAATGGCTGTTCGAAAACGCCATGCACGGTATTTTCCAGGCCAGCCTTCAGGACGGCATGCGTGCCGCCAACCCGGCGCTGGCGCGCATGCTCgg > 6:497645/1‑134 (MQ=255) gcccgccTGGACGAACTGGAAGCCGAACGCAACCGTTACAAATGGATGTTCGAAAACGCCATGCACGGTATTTTCCAGGCCAGCCTTCAGGACGGCATGCGTGCCGCCACCCCGGCGCTGGCGCGCATGCTCgg > 1:103159/1‑134 (MQ=255) gcccgccTGGACGAACTGGAAGCCGAACGCAACAGTTACAAATGGCTGTTCGAAAACGCCATGCACGGTATTTTCCAGGCCAGCCTTCAGGACGCAATGCGTGACGCCAACCCGGAGATGGCGCGAATGCTCgg > 7:29151/1‑134 (MQ=255) gcccgccTGGACGAACTGGAAGCCGAACGAAACCGTTACAAATGGCTGTTCGAAAACGCCATGCACGGTATTTTCCAGGCCAGCCTTCAGGACTGCAAGCGTGCCGCCAACCCGGCGCTGGCGCGCATGCTCgg > 8:107720/1‑134 (MQ=255) cgccTGGACGAACTGGAAGCCGAACGCAACCGTTACAAATGGCTGTTCGAAAACGCCATGCACGGTATTTTCCAGGCCAGCCTTCAGGACGGCATGCGTGCCGCCAACCCGGCGCTGGCGCGCATGCTCGGCTAt < 7:353386/135‑1 (MQ=255) cgccTGGACGAACTGGAAGCCGAACGCAACCGTTACAAATGGCTGTGCGAAAACGCCATGCACGGTATTTTCCAGGCCAGCCTTCAGGAGGGCATGCGTGCCGCCAACCCGGCGCTGGCGCGCATGCTCGGCTAt < 4:224554/135‑1 (MQ=255) aCGAACTGGAAGCCGAACGCAACCGTTACAAATGGCTGTTCGAAAACGCCATGCACGGTATTTTCCAGGCCAGCCTTCAGGACGGCATGCGTGCCGCCAACCCGGCGCTGGCGCGCATGCTCGGCTATGACGAcc < 8:367476/135‑1 (MQ=255) gAACTGGAAGCCGAACGCAACCGTTACAAATGGCTGTTCGAAAACGCCATGCACGGTATTTTCCAGGCCAGCCTTCAGGACGGCATGCGTGCCGCCAACCCGGCGCTGGCGCGCATGCTCGGCTATGACGAccc > 8:315488/1‑134 (MQ=255) aaCTGGAAGCCGAACGCAACCGTTACAAATGGCTGTTCGAAAACGCCATGCACGGTATTTTCCAGGCCAGCCTTCAGGACGGCATGCGTGCCGCCAACCCGGCGCTGGCGCGCATGCTCGGCTATGACGACCCGc < 4:229650/135‑1 (MQ=255) aaCTGGAAGCCGAACGCAACCGTTACAAATGGCTGTTCGAAAACGCCATGCACGGTATTTTCCAGGCCAGCCTTCAGGACGGCATGCGTGCCGCCAACCCGGCGCTGGCGCGCATGCTCGGCTATGACGACCCGc < 5:198851/135‑1 (MQ=255) aaCTGCAACCCGAACGCAACCGTTACAAAGGGCTGTTCCAAAACGCCAGGCACGGTATTTTCCAGGCCAGCCTTCAGGACGGCATGCGGGCCGCCAACCCGGCGCTGGCGCGCATGCTCGGCTATGACGACCCGc < 5:11465/135‑1 (MQ=255) aaGCCGAACGCAACCGTTACAAATGGCTGTTCGAAAACGCCATGCACGGTATTTTCCAGGCCAGCCTTCAGGACGGCATGCGTGCCGCCAACCCGGCGCTGGCGCGCATGCTCGGCTATGACGACCCGCAAGCgg > 7:268862/1‑135 (MQ=255) aaGCCGAACGCAACCGTTACAAATGGCTGTGCGAAAACGCCATGCACGGTATTTTCCAGGCCAGCCTTCAGGACGGCATGCGTGCCGCCAACCCGGCGCTGGCGCGCATGCTCGGCTATGACGACCCGCAAGCgg < 3:33053/135‑1 (MQ=255) cGAACGCAACCGTTACAAATGGCGGTTCGAAAACGCCATGCACGGTATTTTCCAGGCCAGCCTTCAGGACGGCATGCGTGCCGCCAACCCGGCGCTGGCGCGCATGCTCGGCTATGACGACCCGCAAGCGGTGCt < 6:135714/135‑1 (MQ=255) aCGCAACCGTTACAAATGGCTGTGCGAAAACGCCATGCACGGTATTTTCCAGGCCAGCCTTCAGGACGGCATGCGTGCCGCCAACCCGGCGCTGGCGCGCATGCTCGGCTATGACGACCCGCAAGCGGTGCTGtt < 3:308759/135‑1 (MQ=255) cGCAACCGTTACAAATGGCTGTTCGAAAACGCCATGCACGGTATTTTCCAGGCCAGCCTTCAGGACGGCATGCGTGCCGCCAACCCGGCGCTGGCGCGCATGCGCGGCTATGACGACCCGCAAGCGGTGCTGttt < 5:497645/135‑1 (MQ=255) aCCGTTACTAATGGCGGTTCGAAAACGGCATGCACGGTATTTTCCAGGCCAGCCTGCAGGACGGCATGCGTGCCGCCAACCCGGCGCTGGCGCGCATGCTCGGCTATGACGACCCGCAAGCGGTGCTGTTTTccc < 6:504068/135‑1 (MQ=255) aactGGCTGTTCGAAAACGCCATGCACGGTATTTTCCAGGCCAGCCTTCAGGACGGCATGCGTGCCGCCAACCCGGCGCTGGCGCGCATGCTCGGCTATGACGACCCGCAAGCGGTGCTGTTTTCCctgactga < 2:98555/131‑1 (MQ=255) aaaTGGCTGTTCGAAAACGCCATGCACGGTATTTTCCAGGCCAGCCTTCAGGACGGCATGCGTGCCGCCAACCCGGCGCTGGCGCGCATGCTCGGCTATGACGACCCGCAAGCGGTGCTGTTTTCCctgactga < 8:347354/134‑1 (MQ=255) tGGCTGTTCGAAAACGCCATGCACGGTATTTTCCAGGCCAGCCTTCAGGACGGCATGCGTGCCGCCAACCCGGCGCTGGCGCGCATGCTCGGCTATGACGACCCGCAAGCGGTGCTGTTTTCCCTGACTGACCTg < 4:115476/135‑1 (MQ=255) cTGTTCGAAAACGCCATGCACGGTATTTTCCAGGCCAGCCTTCAGGACGGCATGCGTGCCGCCAACCCGGCGCTGGCGCGCATGCTCGGCTATGACGACCCGCAAGCGGTGCTGTTTTCCCTGACTGACCTGgcc < 7:315488/135‑1 (MQ=255) tGTTCGAAAACGCCATGCACGGTATTTTCCAGGCCAGCCTTCAGGACGGCATGCGTGCCGCCAACCCGGCGCTGGCGCGCATGCTCGGCTATGACGACCCGCAAGCGGTGCTGTTTTCCCTGCCTGACCTGgccg > 2:437291/1‑135 (MQ=255) gTTCGAAAACGCCATGCACGGTATTTTCCAGGCCAGCCTTCAGGACGGCATGCGTGCCGCCAACCCGGCGCTGGCGCGCATGCTCGGCTATGACGACCCGCAAGCGGTGCTGTTTTCCCTGACTGACCTGgccgc > 1:214860/1‑135 (MQ=255) ttCGAAAACGCCATGCACGGTATTTTCCAGGCCAGCCTTCAGGACGGCATGCGTGCCGCCAACCCGGCGCTGGCGCGCATGCTCGGCTATGACGACCCGCAAGCGGTGCTGTTTTCCCTGACTGACCTGgccgcc < 8:326153/135‑1 (MQ=255) ttCGAAAACGCCATGCACGGTATTTTCCAGGCCAGCCTTCAGGACGGCATGCGTGCCGCCAACCCGGCGCTGGCGCGCATGCTCGGCTATGACGACCCGCAAGCGGTGCTGTTTTCCCTGACTGACCTGgccgc > 4:503950/1‑134 (MQ=255) tCGAAAACGCCATGCACGGTTTTTTCCAGGCCAGCCTTCAGGACGGCATGCGTGCCGCCAACCCGGCGCTGGCGCGCATGCTCGGCTATGACGACCCGCAAGCGGTGCTGTTTTCCCTGACTGACCTGgccgcc < 7:285313/134‑1 (MQ=255) tCGAAAACGCCATGCACGGTATTTTCCAGGCCAGCCTTCAGGACGGCATGCGTGCCGCCAACCCGGCGCTGGCGCGCATGCTCGGCTATGACGACCCGCAAGCGGTGCTGTTTTCCCTGACTGACCTGgccgcc < 6:397481/134‑1 (MQ=255) gAAAACGCCATGCACGGTATTTTCCAGGCCAGCCTTCAGGACGGCATGCGTGCCGCCAACCCGGCGCTGGCGCGCATGCTCGGCTATGACGACCCGCAAGCGGTGCTGTTTTCCCTGACTGACCTGGCCGCCAAc > 3:260664/1‑135 (MQ=255) aaCGCCATGCACGGTATTTTCCAGGCCAGCCTTCAGGACGGCATGCGTGCCGCCAACCCGGCGCTGGCGCGCATGCTCGGCTATGACGACCCGCAAGCGGTGCTGTTTTCCCTGACTGACCTGGCCGCCAACCTg < 6:542942/135‑1 (MQ=255) cGCCATGCACGGTATTTTCCAGGCCAGCCTTCAGGACGGCATGCGTGCCGCCAACCCGGCGCTGGCGCGCATGCTCGGCTATGACGACCCGCAAGCGGTGCTGTTTTCCCTGACTGACCTGGCCGCCAACCTGtt < 6:392852/135‑1 (MQ=255) | GGGCGTTGGCCGGGCTGCTGGGGCTGGGTGACCACTCGGCGCGTAAAAGCCATTACCCGGAGCTGTCCGCCCGCCTGGACGAACTGGAAGCCGAACGCAACCGTTACAAATGGCTGTTCGAAAACGCCGTGCACGGTATTTTCCAGGCCAGCCTTCAGGACGGCATGCGTGCCGCCAACCCGGCGCTGGCGCGCATGCTCGGCTATGACGACCCGCAAGCGGTGCTGTTTTCCCTGACTGACCTGGCCGCCAACCTGTT > ACB122/3065380‑3065638 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |