Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	ACB122	3,892,845	C→A	intergenic (‑62/‑393)	CJ019_03683 ← / → gapN	hypothetical protein/NADP‑dependent glyceraldehyde‑3‑phosphatedehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	ACB122	3,892,845	0	C	A	100.0%	177.4 / NA	50	intergenic (‑62/‑393)	CJ019_03683/gapN	hypothetical protein/NADP‑dependent glyceraldehyde‑3‑phosphatedehydrogenase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (28/22);  total (28/22)

AGCCTGATTTCGTAGCGCAGGGAGGGCGACAGGAGCTTGCGCGCCACAGTCGTCAAGGTCTTTTTCATTTCTTCTTCGACGTCCTTTGAAGTGCAC‑TGTTGGCCAATCGGAACAAGAGAAAAAACCCGGCAAAAACACTTAACACTTTGATTTTTATTGTTTTACTTGAAACTTTACTATCACATTCAGTAACAATTTAAGATGCCTGCAAACGCTTTGGCAAGCGGCTTCTGGCTGACTTTTGATCAAGTTTCATGG  >  ACB122/3892716‑3892973                                                                                                                                   |                                                                                                                                 agcCTGATTTCGTAGCGCAGGGAGGGCGACAGGAGCTTGCGCGCCACAGTCGTGAAGGTGGTTTTTATTTTTTCTTCGGCGGCCGTTGTAGTGGAC‑TGTTTGGCAATCGGGCCAAGACAAAAAACCCCGAaaaa                                                                                                                              >  5:376302/1‑134 (MQ=255)   ccTGATTTCGTAGCGCAGGGAGGGCGACAGGAGCTTGCGCGCCACAGTCGTCTAGGTGTTTTTGATTTCTTATTGGAGGGCCTGTGAAGTGGAC‑GTTTTGCCAATCAGTACAAGTAAAAAAACCCGGAAAAaaca                                                                                                                           >  2:41709/1‑135 (MQ=255)   ccTGATTTCGTAGCGCAGGGAGGGCGACAGGAGCTTGCGCGCCACAGTCGTCAAGGGGGTTTTGATTTATTGTTCGGCGGGCTTTGTAGTTCACGGGTGGGCCAACCGCAA‑AAGAGAAAAAACCCGGAAAAaac                                                                                                                            >  2:467909/1‑134 (MQ=255)                cgcAGGGAGGGCGACAGGAGCTTGCGCGCCACAGTCGTCAAGGTCGTTTTGATTTATTCTTCGACGGCCGTTGAAGTGGAC‑TGGTGGGCAATCGGAACAAGAGAAAAAACCCGGAAAAAACAATTAACACTTTGa                                                                                                              >  4:467028/1‑135 (MQ=255)                  cAGGGAGGGCGACAGGAGCTTGCGCGCCACAGTCGTCAAGGTCTTTTTCATTTCTTCTTCGACGTCCTTTGAAGTGCAC‑TGTTGGCCAATCGGAACAAGAGAAAAAACCCGGAAAAAACACTTAACACTTTGAtt                                                                                                            <  8:387487/135‑1 (MQ=255)                   agggagggCGACAGGAGCTTGCGCGCCACAGTCGTCAAGGTCTTTTTCATTTCTTCTTCGACGTCCTTTGAAGTGCAC‑TGTTGGCCAATCGGAACAAGAGAAAAAACCCGGAAAAAACACTTAACACTTTGAttt                                                                                                           <  6:447875/135‑1 (MQ=255)                             aCAGGACCGTGCGCGCCACAGTCGTCATGGTCTTTTTCTTTTCTTCTTCGACGTCCTTTGTAGGGCAC‑TGGTGGCCAATCGGAACAAGATAAAAAACCCGGAAAAAAGACTTAAAACTTTGATTTTTATTGtttt                                                                                                 <  8:520296/135‑1 (MQ=255)                                ggAGCTTGCGCGCCACAGTCGTCAAGGTCTTTTTCATTTCTTCTTCGACGTCCTTTGAAGTGCAC‑TGTTGGCCAATCGGAACAAGAGAAAAAACCCGGAAAAAACACTTAACACTTTGATTTTTATTGTTTTACt                                                                                              <  7:235943/135‑1 (MQ=255)                                     ttGCGCGCCACAGTCGTCAAGGTCTTTTTCATTTCTTCTTCGACGTCCTTTGAAGTGCAC‑TGTTGGCCAATCGGAACAAGAGAAAAAACCCGGAAAAAACACTTAACACTTTGATTTTTATTGTTTTACTTGaaa                                                                                         <  4:259133/135‑1 (MQ=255)                                       gcgcgcCACAGTCGTCAAGGTCTTTTTCATTTCTTCTTCGACGTCCTTTGAAGTGCAC‑TGGTGGCCAATCGGAACAAGAGAAAAAACCCGGAAAAAACACTTAACACTTTGATTTTTATTGTTTTACTTGAAACt                                                                                       <  1:525106/135‑1 (MQ=255)                                        cgcgcCACAGTCGTCAAGGTCTTTTTCATTTCTTCTTCGACGTCCTTTGAAGTGCAC‑TGTTGGCCAATCGGAACAAGAGAAAAAACCCGGAAAAAACACTTAACACTTTGATTTTTATTGTTTTACTTGAAACt                                                                                       <  2:351547/134‑1 (MQ=255)                                        cgcgcCACAGTCGTCAAGGTCTTTTTCATTTCTTCTTCGACGTCCTTTGAAGTGCAC‑TGTTGGCCAATCGGAACAAGAGAAAAAACCCGGAAAAAACACTTAACACTTTGATTTTTATTGTTTTACTTGAAACt                                                                                       <  5:201837/134‑1 (MQ=255)                                        cgcgcCACAGTCGTCAAGGTCTTTTTCATTTCTTCTTCGACGTCCTTTGAAGTGCAC‑TGTTGGCCAATCGGAACAAGAGAAAAAACCCGGAAAAAACACTTAACACTTTGATTTTTATTGTTTTACTTGAAACt                                                                                       <  8:453876/134‑1 (MQ=255)                                           gcCAGAGTCGTCATGGTCTTTTTCATTTCTTCTTCGACGTCCTTTGAAGTGCAC‑TGTTGGCCAATCGGAACAAGAGAAAAAACCAGGAAAAAACACTTAACAGTTTGATTTTTATTGTTTTACTTGAAACTTTAc                                                                                   <  3:53142/135‑1 (MQ=255)                                             cacaGTCGTCAAGGTCTTTTTCATTTCTTCTTCGACGTCCTTTGAAGTGCAC‑TGTTGGCCAATCGGAACAAGAGAAAAAACCCGGAAAAAACACTTAACACTTTGATTTTTATTGTTTTACTTGAAACTTTACTa                                                                                 <  1:315514/135‑1 (MQ=255)                                             cacaGTCGTCAACGTCTTTTTCATTTCTTCTTCGACGTCCTTTGAAGTGCAC‑TGTTGGCCAATCGGAACAAGAGAAAAAACCCGGAAAAAACACTTAACACTTTGATTTTTATTGTTTTACTTGAAACTTTACTa                                                                                 <  4:35797/135‑1 (MQ=255)                                               gaGTCGTCAAGGTCTTTTTCATTTCTTCTTCGACGTCCTTTGAAGTGCAC‑TGTTGGCCAATCGGAACAAGAGAAAAAACCCGGAAAAAACACTTAACACTTTGATTTTTATTGTTTTACTTGAAACTTTACTATc                                                                               <  1:280165/134‑1 (MQ=255)                                               caGTCGTCAAGGTCTTTTTCATTTCTTCTTCGACGTCCTTTGAAGTGCAC‑TGTTGGCCAATCGGAACAAGAGAAAAAACCCGGAAAAAACACTTAACACTTTGATTTTTATTGTTTTACTTGAAACTTTACTATc                                                                               <  2:317549/135‑1 (MQ=255)                                               caGTCGTCAAGGGGTTTTTGATTTCTTCTTCGACGTCCGTTGAAGGGGAC‑TGTTGGGCCATCGGAACAAGAGAAAAAACCCGGAAAAAAAACTTAACACTTTGATTTTTATTGTTTTTCTTGAAACTTTACTATc                                                                               >  8:318966/1‑135 (MQ=255)                                                     tcAAGGTCTTTTTCATTTCTTCTTCGACGTCCTTTGAAGTGCAC‑TGTTGGCCAATCGGAACAAGAGAAAAAACCCGGAAAAAACACTTAACACTTTGATTTTTATTGTTTTACTTGAAACTTTACTATCACATTc                                                                         <  7:208166/135‑1 (MQ=255)                                                     tcAAGGTCTTTTTCATTTCTTCTTCGACGTCCTTTGAAGTGCAC‑TGTTGGCCAATCGGAACAAGAGAAAAAACCCGGAAAAAACACTTAACACTTTGATTTTTATTGTTTTACTTGAAACTTTACTATCACATTc                                                                         <  5:390704/135‑1 (MQ=255)                                                        aGGTCTTTTTCATTTCTTCTTCGACGTCCTTTGAAGTGCAC‑TGTTGGCCAATCGGAACAAGAGAAAAAACCCGGAAAAAACACTTAACACTTTGATTTTTATTGTTTTACTTGAAACTTTACTATCACATTCAg                                                                       <  2:147365/134‑1 (MQ=255)                                                               tttCATTTCTTCTTCGACGTCCTTTGAAGTGCAC‑TGTTGGCCAATCGGAACAAGAGAAAAAACCCGGAAAAAACACTTAACACTTTGATTTTTATTGTTTTACTTGAAACTTTACTATCACATTCAGTAACAAt                                                                >  5:524733/1‑134 (MQ=255)                                                                  cATTTCTTCTTCGACGTCCTTTGAAGTGCAC‑TGTTGGCCAATCGGAACAAGAGAAAAAACCCGGAAAAAACACTTAACACTTTGATTTTTATTGTTTTACTTGAAACTTTACTATCACATTCAGTAACAAtt                                                               >  3:520067/1‑132 (MQ=255)                                                                        ttcttcGACGTCCTTTGAAGTGCAC‑TGTTGGCCAATCGGAACAAGAGAAAAAACCCGGAAAAAACACTTAACACTTTGATTTTTATTGTTTTACTTGAAACTTTACTATCACATTCAGTAACAATTTAAGATGcc                                                      >  5:97528/1‑135 (MQ=255)                                                                        ttcttcGACGTCCTTTGAAGTGAAC‑TGTTGGCCAATCGGAACAAGAGAAAAAACCCGGAAAAAACACTTAACACTTTGATTTTTATTTTTTTACTTGAAACTTTACTATCACATTCAGTATCAATTTAAGATGcc                                                      >  1:124129/1‑135 (MQ=255)                                                                               aCGTCCTTTGAAGTGCAC‑GGGTGGCCAATCGGCACAAGAGAAAAAACCCGGAAAAAACACTTAACACTTTGATTTTTATTGTTTTACTTGAAACTTTACTATCACATTCAGTAACAATTTAAGATGCCTGCAAAc                                               <  2:413943/135‑1 (MQ=255)                                                                                              cAC‑TGTTGGCCAATCGGAACAAGAGAAAAAACCCGGAAAAAACACTTAACACTTTGATTTTTATTGTTTTACTTGAAACTTTACTATCACATTCAGTAACAATTTAAGATGCCTGCAAACGCTTTGGCAAGCGGc                                <  8:474636/135‑1 (MQ=255)                                                                                               aC‑TGTTGGCCAATCGGAACAAGAGAAAAAACCCGGAAAAAACACTTAACACTTTGATTTTTATTGtttt                                                                                                 >  2:527066/1‑69 (MQ=255)                                                                                               aC‑TGTTGGCCAATCGGAACAAGAGAAAAAACCCGGAAAAAACACTTAACACTTTGATTTTTATTGtttt                                                                                                 <  1:527066/69‑1 (MQ=255)                                                                                               aC‑TGTTGGCCAATCGGAACAAGAGAAAAAACCCGGAAAAAACACTTAACACTTTGATTTTTATTGTTTTACTTGAAACTTTACTATCACATTCAGTAACAATTTAAGATGCCTGCAAACGCTTTGGCAAGCGGCt                               >  8:202047/1‑135 (MQ=255)                                                                                               aC‑TGTTGGCCAATCGGAACAAGAGAAAAAACCCGGAAAAAACACTTAACACTTTGATTTTTATTGTTTTACTTGAAACTTTACTATCACATTCAGTAACAATTTAAGATGCCTGCAAACGCTTTGGCAAGCGGCt                               >  5:54810/1‑135 (MQ=255)                                                                                                  tGTTGGCCAATCGGAACAAGAGAAAAAACCCGGAAAAAACACTTAACACTTTGATTTTTATTGTTTTACTTGAAACTTTACTATCACATTCAGTAACAATTTAAGATGCCTGCAAACGCTTTGGCAAGCGGCTTc                             >  8:98795/1‑135 (MQ=255)                                                                                                  tGTTGGCCAATCGGAACAAGAGAAAAAACCCGGAAAAAACACTTAACACTTTGATTTTTATTGTTTTACTTGAAACTTTACTATCACATTCAGTAACAATTTAAGATGCCTGCAAACGCTTTGGCAAGCGGCTTc                             >  3:206113/1‑135 (MQ=255)                                                                                                  tGTTGGCCAATCGGAACAAGAGAAAAAACCCGGAAAAAACACTTAACACTTTGATTTTTATTGTTTTACTTGAAACTTTACTATCACATTCAGTAACAATTTAAGATGCCTGCAAACGCTTTGGCAAGCGGCTTc                             >  5:357563/1‑135 (MQ=255)                                                                                                  tGTTGGCCAATCGGAACAAGAGAAAAAACCCGGAAAAAACACTTAACACTTAGATTTTTATTGTTTTACTTGAAACTTTACTATCACATTCAGTAACAATTTAAGATGCCTGCAAACGCTTTGGCAAGCGGCTTc                             >  5:425375/1‑135 (MQ=255)                                                                                                  tGTTGCCCAATCGGAACAAGAGAAAAAACCCGGAAAAAACACTTAACACTTTTATTTTTATTGTATTACTTGAAACTATACTATCACATTCAGTAACAATTTAAGATGCCTGCAAACGCTTTGGCAAGCGGCtac                             >  6:526359/1‑133 (MQ=255)                                                                                                       gCCAATCGGAACAAGAGAAAAAACCCGGAAAAAACACTTAACACTTTGATTTTTATTGTTTTACTTGAAACTTTACTATCACATTCAGTAACAATTTAAGATGCATGCAAACGCTTTGGCAAGCGGATTCTgga                         >  7:307755/1‑133 (MQ=255)                                                                                                          aaTCGGAACAAGAGAAAAAACCCGGAAAAAACACTTAACACTTTGATTTTTATTGTTTTACTTGAAACTTTACTATCACATTCAGTAACAATTTAAGATGCCTGCAAACGCTTTGGCAAGCGGCTTCTGGCTGAc                     >  8:86783/1‑135 (MQ=255)                                                                                                          aaTCGGAACAAGAGAAAAAACCCGCAAAAAACACTTAACACTTTGATTTTTATTGTTTTACTTGAAACTTTACTATCACATTCAGTAACAATTTAAGATGCCTGCAAACGCTTTGGCAAGCGGCTTCTGGCTGAc                     >  1:139868/1‑135 (MQ=255)                                                                                                            tCGGAACAAGAGAAAAAACCCGTAAAAAACACTTAACACTTTGATTTTTATTGTTTTACTTGAAACTTTACTATCACATTCAGTAACAATTTAAGATGCCTGCAAACGCTTTGGCAAGCTGCTTCTGGCTGACtt                   >  6:43011/1‑135 (MQ=255)                                                                                                               gAACAAGAGAAAAAACCCGGAAAAAACACTTAACACTTTGATTTTTATTGTTTTACTTGAAACTTTACTATCACATTCAGTAACAATTTAAGATGCCTGCAAACGCTTTGGCAAGCGGCTTCTGGCTGACTTTTg                <  5:460948/135‑1 (MQ=255)                                                                                                               gAACAAGAGAAAAAACCCGGAAAAAACACTTAACACTTTGATTTTTATTGTTTTACTTGAAACTTTACTATCACATTCAGTAACAATTTAAGATGCCTGCAAACGCTTTGGCAAGCGGCTTCTGGCTGACTTTTg                >  2:74916/1‑135 (MQ=255)                                                                                                               gAACAAGAGAAAAAACCCGGAAAAAACACTTAACACTTTGATTTTTATTGTTTTACTTGAAACTTTACTATCACATTCAGTAACAATTTAAGATGCCTGCAAACGCTTTGGCAAGCGGCTTCTGGCTGACTTTTg                >  2:252117/1‑135 (MQ=255)                                                                                                                       gaAAAAACCCGGAAAAAACACTTAACACTTTGATTTTTATTGTTTTACTTGAAACTTTACTATCACATTCAGTAACAATTTAAGATGCCTGCAAACGCTTTGGCAAGCGGCTTCTGGCTGACTTTTGATCAAGtt        <  1:193419/135‑1 (MQ=255)                                                                                                                        aaaaaaCCCGGAAAAAACACTTAACACTTTGATTTTTATTGTTTTACTTGAAACTTTACTATCACATTCAGTAACAATTTAAGATGCCTGCAAACGCTTTGGCAAGCGGCTTCTGGCTGACTTTTGATCAAGtt        >  6:498166/1‑134 (MQ=255)                                                                                                                             aCCCGGAAAAAACACTTAACACTTTGATTTTTATTGTTTTACTTGAAACTTTACTATCACATTCAGTAACAATTTAAGATGCCTGCAAACTCTTTGGCAAGCGGCTTCTGGCTGACTTTTGATCAAGTTTCATgg  >  4:351952/1‑135 (MQ=255)                                                                                                                             aCCCGGAAAAAACACTTAACACTTTGATTTTTATTGTTTTACTTGAAACTTTACTATCACATTCAGTAACAATTTAAGATGCCTGCAAACGCTTTGGCAAGCGGCTTCTGGCTGACTTTTGATCAAGTTTCATgg  >  4:368040/1‑135 (MQ=255)                                                                                                                             aCCCGGAAAAAACACTTAACACTTTGATTTTTATTGTTTTACTTGAAACTTTACTATCACATTCAGTAACAATTTAAGATGCCTGCAAACGCTTTGGCAAGCGGCTTCTGGCTGACTTTTGATCAAGTTTCATgg  >  4:245816/1‑135 (MQ=255)                                                                                                                             aCCCGGAAAAAACACTTAACACTTTGATTTTTATTGTTTTACTTGAAACTTTACTATCACATTCAGTAACAATTTAAGATGCCTGCAAACGCTTTGGCAAGCGGCTTCTGGCTGACTTTTGATCAAGTTTCATgg  >  2:206490/1‑135 (MQ=255)                                                                                                                                   |                                                                                                                                 AGCCTGATTTCGTAGCGCAGGGAGGGCGACAGGAGCTTGCGCGCCACAGTCGTCAAGGTCTTTTTCATTTCTTCTTCGACGTCCTTTGAAGTGCAC‑TGTTGGCCAATCGGAACAAGAGAAAAAACCCGGCAAAAACACTTAACACTTTGATTTTTATTGTTTTACTTGAAACTTTACTATCACATTCAGTAACAATTTAAGATGCCTGCAAACGCTTTGGCAAGCGGCTTCTGGCTGACTTTTGATCAAGTTTCATGG  >  ACB122/3892716‑3892973

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑