Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	ACB122	3,957,240	C→T	G137R (GGG→AGG)	dmlR_13 ←	HTH‑type transcriptional regulator DmlR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	ACB122	3,957,240	0	C	T	100.0%	342.4 / NA	98	G137R (GGG→AGG)	dmlR_13	HTH‑type transcriptional regulator DmlR
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (51/47);  total (51/47)

CTCCGGTACACCGTGGGTGTCCAGGTAGCCGGGGGCCGCGCAGGGGATGCTGAAAATATTGCCCAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCCTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTGCGGGCAGTGCAAACGCAG  >  ACB122/3957112‑3957369                                                                                                                                 |                                                                                                                                  cTCCGGTACACCGTGGGTGTCCAGGTAGCCGGGGGCCGCGCAGGGGATGCTGAAAATATTGCCCAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCTTTCGGc                                                                                                                             <  5:481226/135‑1 (MQ=255) cTCCGGTACACCGTGGGTGTCCAGGTAGCCGGGGGCCGCGCAGGGGATGCTGAAAATATTGCCCAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCTTTCGGc                                                                                                                             <  8:514316/135‑1 (MQ=255) cTCCGGTACACCGTGGGTGTCCAGGTAGCCGGGGGCCGCGCAGGGGATGCTGAAAATATTGCCCAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCTTTCGGc                                                                                                                             <  8:25756/135‑1 (MQ=255) cTCCGGTACACCGTGGGTGTCCAGGTAGCCGGGGGCCGCGCAGGGGATGCTGAAAATATTGCCCAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCTTTCGGc                                                                                                                             <  3:326218/135‑1 (MQ=255) cTCCGGTACACCGTGGGTGTCCAGGTAGCCGGGGGCCGCGCAGGGGATGCTGAAAATATAGCCCAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCTTTCGGc                                                                                                                             <  3:25358/135‑1 (MQ=255) cTCCGGTACACCGTGGGTGTCCAGGTAGCCGGGGGCAGCGCAGGGGATTCTGAAAATATTGCCCAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCTTTCGGc                                                                                                                             <  3:283508/135‑1 (MQ=255)  tCCGGTACACCGTGGGTGTCCAGGTAGCCGGGGGCCGCGCAGGGGATGCTGAAAATATTGCCCAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCTTTCgg                                                                                                                              >  5:442783/1‑133 (MQ=255)    cGGTACACCGTGGGTGTCCAGGTAGCCGGGGGCCGCGCAGGGGATGCTGAAAATATTGCCCAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCTTTCGGCCAg                                                                                                                          >  3:468868/1‑135 (MQ=255)      gTACACCGTGGGTGTCCAGGTAGCCGGGGGCCGCGCAGGGGATGCTGAAAATATTGCCCAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCTTTCGGCCAGCg                                                                                                                        <  1:390153/135‑1 (MQ=255)      gTACACCGTGGGTGTCCAGGTAGCCGGGGGCCGCGCAGGGGATGCTGAAAATATTGCCCAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCTTTCGGCCAGCg                                                                                                                        <  5:437265/135‑1 (MQ=255)      gTACACCGAGGGTGTCCAGGTAGCCGGGGGCCGCGCAGGGGATGCTGAAAATATTGCCCAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCCCGGTGATCACCACGTCATGCCTTTCGGCCAGCg                                                                                                                        <  7:312550/135‑1 (MQ=255)               gggTGTCCAGGTAGCCGGGGGCCGCGCAGGGGATGCTGAAAATATTGCCCAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCTTTCGGCCAGCGGGTCGGGGt                                                                                                               >  7:363453/1‑135 (MQ=255)               gggTGTCCAGGTAGCCGGGGGCCGCGCAGGGGATGCTGAAAATATTGCCCAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCTTTCGGCCAGCGGGTCGGGGt                                                                                                               >  2:290209/1‑135 (MQ=255)               gggTGTCCAGGTAGCCGGGGGCCGCGCAGGGGATGCTGAAAATATTGCCCAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCTTTCGGCCAGCGGGTCGGGGt                                                                                                               >  7:253877/1‑135 (MQ=255)               gggTGTCCAGGTAGCCGGGGGCCGCGCAGGGGATGCTGAAAATATTGCCCAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCTTTCGGCCAGCGGGTCGGGGt                                                                                                               >  5:334046/1‑135 (MQ=255)               gggTGTCCAGGTAGCCGGGGGCCGCGCAGGGGATGCTGAAAATATTGCCCAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCTTTCGGCCAGCGGGTCGGGGt                                                                                                               >  4:274276/1‑135 (MQ=255)               gggTGTCCAGGTAGCCGGGGGCCGCGCAGGGGATGCTGAAAATATTGCCCAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCTTTCGGCCAGCGGGTCGGGGt                                                                                                               >  7:108533/1‑135 (MQ=255)               gggTGTCCAGGTAGCCGGGGGCCGCGCAGGGGATGCTGAAAATATTGCCCAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCTTTCGGCCAGCGGGTCGGGGt                                                                                                               >  1:520461/1‑135 (MQ=255)               gggTGTCCAGGTAGCCGGGGGCCGCGCAGGGGATGCTGAAAATATTGCCCAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCTTTCGGCCAGCGGGTCGGGGt                                                                                                               >  5:514644/1‑135 (MQ=255)               gggTGTCCAGGTAGCCGGGGGCCGCGCAGGGGATGCTAAAAATATTGCCCAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCTTTCGGCCAGCGGGTCGGGGt                                                                                                               >  5:503955/1‑135 (MQ=255)                 gtgtCCAGGTAGCCGGGGGCCGCGCAGGGGATGCTGAAAATATTGCCCAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCCCCGTCCACGGTGATCACCACGTCATGCCTTTCGGCCAGCGGGGCGGGGTTg                                                                                                             >  1:449361/1‑135 (MQ=255)                 gtgtCCAGGTAGCCGGGGGCCGCGCAGGGGATGCTGAAAATATTGCCCAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCCCGTCATGCCTTTCGGCCAGCGGGTCGGGGTTg                                                                                                             >  1:135488/1‑135 (MQ=255)                 gtgtCCAGGTAGCCGGGGGCCGCGCAGGGGATGCTGAAAATATTGCCCAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCTTTCGGCCAGCGGGTCGGGGtt                                                                                                              >  7:304446/1‑134 (MQ=255)                 gtgtCCAGGTAGCCGGGGGCCGCGCAGGGGATGCTGAAAATATTGCCCAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCTTTCGGCCAGCGGGTCGGGGtt                                                                                                              <  8:304446/134‑1 (MQ=255)                 gtgtCCAGGTAGCCGGGGGCCGCGCAGGGGATGCTGAAAATATTGCCCAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCTTTCGGCCAGCGGGTCGGGGTTg                                                                                                             >  4:160006/1‑135 (MQ=255)                 gtgtCCAGGTAGCCGGGGGCCGCGCAGGGGATGCTGAAAATATTGCCCAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCTTTCGGCCAGCGGGTCGGGGTTg                                                                                                             >  5:191940/1‑135 (MQ=255)                 gtgtCCAGGTAGCCGGGGGCCGCGCAGGGGATGCTGAAAATATTGCCCAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCTTTCGGCCAGCGGGTCGGGGTTg                                                                                                             >  3:81321/1‑135 (MQ=255)                 gtgtCCAGGTAGCCGGGGGCCGCGCAGGGGATGCTGAAAATATTGCCCAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCTTTCGGCCAGCGGGTCGGGGTTg                                                                                                             >  6:104288/1‑135 (MQ=255)                 gtgtCCAGGTAGCCGGGGGCCGCGCAGGGGATGCTGAAAATATTGCCCAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCTTTCGGCCAGCGGGTCGGGGTTg                                                                                                             >  2:395495/1‑135 (MQ=255)                 gtgtCCAGGTAGCCGGGGGCCGCGCAGGGGATGCTGAAAATATTGCCCAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCTTTCGGCCAGCGGGTCGGGGTTg                                                                                                             >  7:413241/1‑135 (MQ=255)                 gtgtCCAGGTAGCCGGGGGCCGCGCAGGGGATGCTGAAAATATTGCCCAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCTTTCGGCCAGCGGGTCGGGGTTg                                                                                                             >  8:291706/1‑135 (MQ=255)                 gtgtCCAGGTAGCCGGGGGCCGCGCAGGGGATGCTGAAAATATTGCCCAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCTTTCGGCCAGCGGGTCGGGGTTg                                                                                                             >  1:272759/1‑135 (MQ=255)                  tgtCCAGGTAGCCGGGGGCCGCGCAGGGGATGCTGAAAATATTGCCCAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCTTTCGGCCAGCg                                                                                                                        <  6:179756/123‑1 (MQ=255)                  tgtCCAGGTAGCCGGGGGCCGCGCAGGGGATGCTGAAAATATTGCCCAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCTTTCGGCCAGCg                                                                                                                        >  5:179756/1‑123 (MQ=255)                   gtCCAGGTAGCCGGGGGCCGCGCAGGGGATGCTGAAAATATTGCCCAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCTTTCGGCCAGCGGGTCGGGGTTgcg                                                                                                           >  1:25870/1‑135 (MQ=255)                       aGGTAGCCGGGGGCCGCGCAGGGGATGCTGAAAATATTGCCCAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCTTTCGGCCAGCGGGTCGGGGTTGCGCTg                                                                                                        <  5:104288/134‑1 (MQ=255)                       aGGTAGCCGGGGGCCGCGCAGGGGATGCTGAAAATATTGCCCAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCTTTCGGCCAGCGGGTCGGGGTTGCGCTg                                                                                                        <  8:108533/134‑1 (MQ=255)                            gCCGGGGGCCGCGCAGGGGATGCTGAAAATATTGCCCAGCGGCACAGCGATCAGTTGCGACTCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCTTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAg                                                                                                  >  6:5946/1‑135 (MQ=255)                             ccGGGGGCCGCGCAGGGGATGCTGAAAATATTGCCCAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCTTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGc                                                                                                 <  7:15446/135‑1 (MQ=255)                                   gCCGCGCAGGGGATGCTGAAAATATTGCCCAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCTTTCGGCCAGCGGTTCGGGGTTGCGCTGCGACAGCGTCAGc                                                                                           <  5:25313/135‑1 (MQ=255)                                        gcAGGGGATGCTTAAAATATTGCCCAGCGGCACAGCGATCAGTTGCTAATCCGGCAGCGCACCGTCCACGGTTATCACCACGTCATGCCTTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCTAt                                                                                      <  7:461799/135‑1 (MQ=255)                                        gcAGGGGATGCTGAAAATATTGCCCAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCTTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGAt                                                                                      <  7:291706/135‑1 (MQ=255)                                        gcAGGGGATGCTGAAAATATTGCCCAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCTTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGAt                                                                                      <  6:422765/135‑1 (MQ=255)                                        gcAGGGGATGCTGAAAATATTGCCCAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCTTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGAt                                                                                      <  5:200754/135‑1 (MQ=255)                                          aGGGGCTGCTGAAAATATTGCCCAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCTTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGGTCGATca                                                                                    >  6:338050/1‑135 (MQ=255)                                             ggATGCTGAAAATATTGCCCAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCTTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATcacca                                                                                 <  5:140481/135‑1 (MQ=255)                                                    gAAAATATTGCCCAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCTTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCgg                                                                          >  1:55116/1‑135 (MQ=255)                                                    gAAAATATTGCCCAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCTTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCgg                                                                          >  2:466993/1‑135 (MQ=255)                                                      aaaTATTGCCCCGCGGCACAGCGAGCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCTTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCgg                                                                          <  6:388034/133‑1 (MQ=255)                                                      aaaTATTGCCCAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCTTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCgg                                                                          <  1:470257/133‑1 (MQ=255)                                                        atatTGCCCAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCTTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTaa                                                                      <  2:129077/135‑1 (MQ=255)                                                         tatTGCCCAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCTTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTaaa                                                                     >  5:514657/1‑135 (MQ=255)                                                              cccAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCTTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCAtg                                                                >  7:219801/1‑135 (MQ=255)                                                               ccAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCTTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCCGCTCGATCACCACTTCCGGGTAAAGCAtgt                                                               >  1:449786/1‑135 (MQ=255)                                                               ccAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCTTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCAtgt                                                               >  4:371994/1‑135 (MQ=255)                                                                  gCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCTTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCAtgttgt                                                            >  2:526309/1‑135 (MQ=255)                                                                      cacaGGTATAAGTTGCGAATCCGGGAGCTCACCGTCCACTGTTATCACCACGTCTTGCCTTTCGGCCAGCTGTTCGGGGTTGCGCTGCGACAGCGTCTGCTGGATCACCACTTCCGGGTAAAGCATGTTGTAGcc                                                        <  4:54262/135‑1 (MQ=255)                                                                            gATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCTTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGccac                                                  <  8:404179/135‑1 (MQ=255)                                                                              tCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCTTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGccacca                                                >  8:136959/1‑135 (MQ=255)                                                                                agatGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCTTTCGGCCAGCGGCTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGGAAAGCATGTTTAAGCCGGCCACCAGc                                              >  7:221790/4‑135 (MQ=255)                                                                                 gTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCTTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCg                                             <  5:218070/135‑1 (MQ=255)                                                                                   tGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCTTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAACGCATGTTGTAGCCGGCCACCAGCGGc                                           <  2:232155/135‑1 (MQ=255)                                                                                   tGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCTTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGc                                           <  6:277189/135‑1 (MQ=255)                                                                                   tGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCTTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGc                                           <  4:81321/135‑1 (MQ=255)                                                                                        agccGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCTTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAg                                      <  6:333439/133‑1 (MQ=255)                                                                                        aTCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCGTTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCCGCTCGATCACCACTCCCGGGGAAAGCAGGTTGTAGCCGCCCACCCGCGGCATct                                       >  1:529967/1‑133 (MQ=255)                                                                                        aTCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCTTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAg                                      <  6:113060/135‑1 (MQ=255)                                                                                        aTCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCTTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAg                                      <  2:521119/135‑1 (MQ=255)                                                                                        aTCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCTTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAg                                      <  1:395495/135‑1 (MQ=255)                                                                                        aTCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCTTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGAGCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAg                                      <  5:446426/135‑1 (MQ=255)                                                                                        aTCCGGCAGCGCACCGTCCACGGTGATCACCACGGCATGCCTTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAg                                      <  3:152817/135‑1 (MQ=255)                                                                                          ccGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCTTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCa                                    >  3:336936/1‑135 (MQ=255)                                                                                            ggCAGCGCACCGTCCACGGTGATCACCACGTCATGCCTTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAAc                                  <  2:484506/135‑1 (MQ=255)                                                                                             gCAGCGCACCGTCCACGGTGAGCACCACGTCATGCCTTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACa                                 >  3:468357/1‑135 (MQ=255)                                                                                               aGCGCACCGTCCACGGTGATCTCCACGTCATGCCTTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGc                               <  2:483104/135‑1 (MQ=255)                                                                                                 cgcACCGTCCACGGTGATCACCACGTCATGCCTTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGccc                             <  7:136959/135‑1 (MQ=255)                                                                                                   cACCGTCCACGGTGATCACCACGTCATGCCTTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCAGCAGCGGCATCAGCAACAGCCCCa                           <  6:494614/135‑1 (MQ=255)                                                                                                           aCGGTGATCACCACGTCATGCCTTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTGc                   <  6:442783/135‑1 (MQ=255)                                                                                                            cGGTGATCACCACGTCTTGCCTTTGGGCCTGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCGCGTTCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACTGCCCCATGCCGTGCg                  <  6:478075/135‑1 (MQ=255)                                                                                                            cGGTGATCACCACGTCATGCCTTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTGCg                  <  6:514657/135‑1 (MQ=255)                                                                                                            cGGTGATCACCACGTCATGCCTTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTGCg                  <  2:418714/135‑1 (MQ=255)                                                                                                            cGGGGATCACCACGTCATGCCTTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCAACAGCGGCATCAGCAACAGCCCCATGCCGTGCg                  <  2:272759/135‑1 (MQ=255)                                                                                                              gTGATCACCACGTCATGCCTTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTGCgg                 >  8:52112/1‑134 (MQ=255)                                                                                                                   caccacGTCATGCCTTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTGCGGGCAGt            >  8:189233/1‑134 (MQ=255)                                                                                                                     ccacGTCATGCCTTTCGGCCAGCGGGTCGGGGTTGCGCTGCGCCAGCGTCAGCTCGATCACCACGTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCACCAGCCCCATGCCGTGAGGGCAGTGc          >  5:380197/1‑134 (MQ=255)                                                                                                                     ccacGTCATGCCTTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTGCGGGCAGTGc          >  7:268680/1‑134 (MQ=255)                                                                                                                     ccacGTCATGCCTTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTGCGGGCAGTGc          >  6:107268/1‑134 (MQ=255)                                                                                                                     ccacGTCATGCCTTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTGCGGGCAGTGc          >  5:281874/1‑134 (MQ=255)                                                                                                                     ccacGTCATGCCTTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTGCGGGCAGTGc          >  4:59577/1‑134 (MQ=255)                                                                                                                     ccacGTCATGCCTTTATGCCAGATTGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTGCGGGCAGTGc          >  3:517332/1‑134 (MQ=255)                                                                                                                     ccacGTCATGCCTTTAGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTGCGGGCAGTGc          >  6:358955/1‑134 (MQ=255)                                                                                                                       acGTCATGCCTTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTGCGGGCAGTGCaaa       >  3:134312/1‑135 (MQ=255)                                                                                                                            aTGCCTTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGc                                           <  3:152575/94‑1 (MQ=255)                                                                                                                            aTGCCTTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGc                                           >  4:152575/1‑94 (MQ=255)                                                                                                                            aTGCCTTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTGCGGGCAGTGCAAAc      >  7:302579/1‑131 (MQ=255)                                                                                                                             tGCCTTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTGCGGGCAGTGCAAACGCAg  <  3:274276/134‑1 (MQ=255)                                                                                                                              gCCTTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCgg                                            >  2:322789/1‑91 (MQ=255)                                                                                                                              gCCTTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCgg                                            <  1:322789/91‑1 (MQ=255)                                                                                                                                 |                                                                                                                                  CTCCGGTACACCGTGGGTGTCCAGGTAGCCGGGGGCCGCGCAGGGGATGCTGAAAATATTGCCCAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCCTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTGCGGGCAGTGCAAACGCAG  >  ACB122/3957112‑3957369

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑