Predicted mutation
evidence seq id position mutation annotation gene description
RA ACB122 4,045,783 C→T G101D (GGC→GAC)  argE_1 ← Acetylornithine deacetylase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1224,045,7830CT89.9% 157.1 / NA 70G101D (GGC→GAC) argE_1Acetylornithine deacetylase
Reads supporting (aligned to +/- strand):  ref base C (0/1);  major base T (22/40);  minor base G (0/7);  total (22/48)
Fisher's exact test for biased strand distribution p-value = 8.81e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GAGCGCACGCCCAAACAACCGACTTCTTCGTCATAGGAAAACGCCAGGTGTACCGGCATGCGCAACGGCTGCGCGAGAAACGCGGGCACAGCAGCCAACACCGAGGCGATAAAGCCTTTCATGTCGGCCGTGCCGCGGCCATACAGGCGGCCGTCACGCTCACTCAGGGCAAACGGCTCGACTGTCCAGGCCTGGCCATCCACCGGCACCACATCGGTATGCCCAGACAGCACCACACCACCAACATCCCTGGGGCCGACAGTG  >  ACB122/4045651‑4045914
                                                                                                                                    |                                                                                                                                   
gaccccACGCCCAACCACCCCACTACTACCTCAACCCAAAACGCCAGGGGAACCGGCATGCGCAACGGCTGCGCGAGAAACGCGGGCACAGCAGCCAACACCGAGGCGATAAAGCCTTTCATGTCGGCCGTGTcg                                                                                                                                   <  6:303501/130‑1 (MQ=255)
   cgcACGCCCAAACAACCGACTTCTTCGTCATAGGAAAACGCCAGGTGTACCGGCATGCGCAACGGCTGCGCGAGAAACGCGGGCACAGCAGCCAACACCGAGGCGATAAAGCCTTTCATGTCGGCCGTGTCGCgg                                                                                                                                >  6:371612/1‑135 (MQ=255)
          ccAAACAACCGACTTCTTCGTCATAGGAAAACGCCAGGTGTACCGGCATGCGCAACGGCTGCGCGAGAAACGCGGGCACAGCAGCCAACACCGAGGCGATAAAGCCTTTCATGTCGGCCGTGTCGCGGCCATACa                                                                                                                         <  5:94929/135‑1 (MQ=255)
            aaaCAACCGACTTCTTCGTCATAGGAAAACGCCAGGTGTACCGGCATGCGCAACGGCTGCGCGAGAAACGCGGGCACAGCAGCCAACACCGAGGCGATAAAGCCTTTCATGTCGGCCGTGTCGCGGCCATACAgg                                                                                                                       <  8:76236/135‑1 (MQ=255)
                  ccGACTGCTTCGTCATAGGAAAACGCCAGGTGTACCGGCATGCGCAACGGCTGCGCGAGAAACGCGGGCACAGCAGCCAACTCCGAGGCGATAAAGCCTTTCATGTCGGCCGTGTCGCGGCCATACAGGCGGCCg                                                                                                                 <  8:189183/135‑1 (MQ=255)
                    gACTTCTTCGTCATAGGAAAACGCCAGGTGTACCGGCATGCGCAACGGCTGCGCGAGAAACGCGGGCACAGCAGCCAACACCGAGGCGATAAAGCCTTTCATGTCGGCCGTGTCGCGGCCATACAGGCGGCCGTc                                                                                                               <  5:146345/135‑1 (MQ=255)
                     aCTTCTTCGTCATAGGAAAACGCCAGGTGTACCGGCATGCGCAACGGCTGCGCGAGAACCGCGGGCACAGCAGCCAACACCGAGGCGATAAAGCCTTTCATGTCGGCCGTGTCGCGGCCCTACAGGCGGCCGTCa                                                                                                              >  3:40487/1‑135 (MQ=255)
                     aCTTCTTCGTCATAGGAAAACGCCAGGTGTACCGGCATGCGCAACGGCTGCGCGAGAAACGCGGGCACAGCAGCCAACACCGAGGCGATAAAGCCTTTCATGTCGGCCGTGTCGCGGCCATACAGGCGGCCGTCa                                                                                                              <  1:133842/135‑1 (MQ=255)
                     aCTTCTTCGTCATAGGAAAACGCCAGGTGTACCGGCATGCGCAACGGCTGCGCGAGAAACGCGGGCACAGCAGCCAACACCGAGGCGATAAAGCCTTTCATGTCGGCCGTGTCGCGGCCATACAGGCGGCCGTCa                                                                                                              >  7:261593/1‑135 (MQ=255)
                            cGTCATAGGAAAACGCCAGGTGTACCGGCATGCGCAACGGCTGCGCGAGAAACGCGGGCACAGCAGCCAACACCGAGGCGATAAAGCCTTTCATGTCGGCCGTGTCGCGGCCATACAGGCGGCCGTCACGctcac                                                                                                       >  7:284946/1‑135 (MQ=255)
                             gTCATAGGAAAACGCCAGGTGTACCGGCATGCGCAACGGCTGCGCGAGAAACGCGGGCACAGCAGCCAACACCGAGGCGATAAAGCCTTTCATGTCGGCCGTGTCGCGGCCATACAGGCGGCCGTCACGctc                                                                                                         <  4:512939/132‑1 (MQ=255)
                             gTCATAGGAAAACGCCAGGTGTACCGGCATGCGCAACGGCTGCGCGAGAAACGCGGGCACAGCAGCCAACACCGAGGCGATAAAGCCTTTCATGTCGGCCGTGTCGCGGCCATACAGGCGGCCGTCACGctc                                                                                                         <  3:417240/132‑1 (MQ=255)
                               cATAGGAAAACGCCAGGTGTACCGGCATGCGCAACGGCTGCGCGAGAAACGCGGGCACAGCAGCCAACACCGAGGCGATAAAGCCTTTCATGTCGGCCGTGTCGCGGCCATACAGGCGGCCGTCACGctcactca                                                                                                    >  8:45142/1‑135 (MQ=255)
                                      acaCGCCAGGGGTCCCGGCAGGCGCACCGGCTGCGCTAGAAACGCGGGCACAGCAGCCAACACCGTGGCTATAAAGCCTTTCATGTCGGCGGTGGCGCGGCCATACAGGCGGCCGTCACGCTCACTCCGGGCaaa                                                                                             <  2:227926/133‑1 (MQ=255)
                                       aaCGCCAGGTGTACCGGCATGCGCAACGGCTGCGCGAGAAACGCGGGCACAGCAGCCAACACCGAGGCGATAAAGCCTTTCATGTCGGCCGTGTCGCGGCCATACAGGCGGCCGTCACGCTCACTCAGGGCAAAc                                                                                            >  3:134844/1‑135 (MQ=255)
                                           cccggTGTCCCGGCATCCCCACCGGCTGCGCGAGAAACGCGGGCACAGCACCCAACACCGAGGCCATAAAGCCTTTCATGGCGGCCGTGTCGCGGCCATACAGGCGGCCGTCACGCTCACTCAGGGCAAACGGCt                                                                                        <  6:76315/132‑1 (MQ=255)
                                           cccggTGTACCGGCATGCGCACCGGCTGCGCGAGAACCGCGGGCACAGCCGCCAACACCGAGGCGATAAAGCCTTTCATGGCGGCCGTGTCGCGGCCATACAGGCGGCCGGCACGCTCACTCAGGGCAAACGGCt                                                                                        <  4:39678/132‑1 (MQ=255)
                                           cccggTGTACCGGCATGCGCAACGGCTGCGCGAGAAACGCGGGCACAGCAGCCAACACCGAGGCGATAAAGCCTTTCATGGCGGCCGTGTCGCGGCCATACAGGCGGCCGTCACGCTCACTCAGGGCAAACGGCt                                                                                        <  6:141307/132‑1 (MQ=255)
                                           ccAGGTGTACCGGCATGCGCAACGGCTGCGCGAGAAACGCGGGCACAGCAGCCAACACCGAGGCGATAAAGCCTTTCATGTCGGCCGTGTCGCGGCCATACAGGCGGCCGTCACGCTCACTCAGGGCAAACGGCt                                                                                        <  2:373091/135‑1 (MQ=255)
                                           ccAGGTGTACCGGCATGCGCAACGGCTGCGCGAGAAACGCGGGCACAGAAGCCAACACCGAGGCGATAAAGCCTTTCATGTCGGCCGTGTGGCGGCCATACAGGCGGCCGTCACGCTCACTCAGGGCAAACGGCt                                                                                        <  4:437716/135‑1 (MQ=255)
                                                tgtTCCGGCATGCGCAACGGCTGCGGGAGAAACGCGGGGACAGCAGCCAACACCGAGGCGATAAAGCCTTTCATGTCGGCGGTGGCGCGGCCATACAGGCGGCCGTCACGCTCACTCAGGGCATACGGGTCGACt                                                                                   <  7:124848/135‑1 (MQ=255)
                                                tgtCCCGGCATGCGCAACGGGGGCGAGAGAAACGCGGGCACAGCAGCCAACACCGAGGCTATAACGCCTTTCATGGCGGCCGTGTCGCGGCCATCCCGGCGGCCGTCACGCTCACTCAGGCCAAACGGCTCGACt                                                                                   <  4:289882/135‑1 (MQ=255)
                                                tgtCCCGGCATGCGCAACGGCTGCGCGAGAAACGCGGGCACAGCAGCCAACACCGAGGCGATAAAGCCGTTCATGTCGGCCGGGTCGCGGCCATCCAGGCGGCCGTCACGCTCACTCAGGGCAAACGGCTCGACt                                                                                   <  7:131404/135‑1 (MQ=255)
                                                tgtACCGGCATGCGCAACGGCTGCGCTAGAAACGCGGGCACTGCAGCCAACACCGAGGCTATAAAGCCTTTCATGTCGGCCGTGTCGCGGCCATACAGGCGGCCGTCACGCTCACTCAGGGCAAACGGCTCGACt                                                                                   <  5:245990/135‑1 (MQ=255)
                                                      ggCATGCGCAACGGCTGCGCGAGAAACGCGGGCACAGCAGCCAACACCGAGGCGATAAAGCCTTTCATGTCGGCCGTGTCGCGGCCATACAGGCGGCCGTCACGCTCCCTCAGGGCAAACGGCTCGACTGTCCAg                                                                             >  5:246486/1‑135 (MQ=255)
                                                      ggCATGCGCAACGGCTGCGCGAGAAACGCGGGCACAGCAGCCAACACCGAGGCGATAAAGCCTTTCATGTCGGCCGTGTCGCGGCCATACAGGCGGCCGTCACGCTCACTCAGGGCAAACGGCTCGACTGTCCAg                                                                             <  6:365821/135‑1 (MQ=255)
                                                       gCATGCGCAACGGCTGCGCGAGAAACGCGGGCACAGCAGCCAACACCGAGGCGATAAAGCCTTTCATGTCGGCCGTGTCGCGGCCATACAGGCGGCCGTCACGCTCACTCAGGGCAAACGGCTCGACTGTCCAgg                                                                            >  8:80630/1‑135 (MQ=255)
                                                            cgcAACGGCTGCGCGAGAAACGCGGGCACAGCAGCCAACACCGAGGCGATAAAGCCTTTCATGTCGGCCGTGTCGCGGCCCTACAGGCGGCCCTCACGCTCACTCAGGGCAAACGGCTCGACTGTCCCGGCCTgg                                                                       >  1:57035/1‑135 (MQ=255)
                                                             gcAACGGCTGCGCGAGAAACGCGGGCACAGCAGCCAACACCGAGGCGATAAAGCCTTTCATGTCGGCCGTGTCGCGGCCATACAGGCGGCCGTCACGCTCACTCAGGGCAAACGGCTCGACTGTCCAGGCCTgg                                                                       >  3:276294/1‑134 (MQ=255)
                                                             gcAACGGCTGCGCGAGAAACGCGGGCACAGCAGCCAACACCGAGGCGATAAAGCCTTTCATGTCGGCCGTGTCGCGGCCATACAGGCGGCCGTCACGCTCACTCAGGGCAAACAGCTCGACTGTCCAGGCCTgg                                                                       >  7:76046/1‑134 (MQ=255)
                                                             gcAACGGCTGCGCGAGAAACGCGGGCACAGCAGCCAACACCGAGGCGATAAAGCCTTTCATGTCGGCCGTGTCGCGGCCATACAGGCCGCCCTCCCCCACACAGACGGCAAACGGCACGCCTGGACCGGCCGGGc                                                                      >  3:289882/1‑135 (MQ=255)
                                                              cAACGGCTGCGCGAGAAACGCGGGCACAGCAGCCAACACCGAGGCGATAAAGCCTTTCATGTCGGCCGTGTCGCGGCCATACAGGCGGCCGTCCCGCTCACTCAGGGCAAACGGCTCGACTGTCCAGGCCTGGcc                                                                     >  2:349458/1‑135 (MQ=255)
                                                                 cGGCTGCGCGAGAAACGCGGGCACAGCAGCCAACACCGAGGCGATAAAGCCTTTCATGTCGGCCGGGGCGCGGCCATACAGGCGGCCGTCACGCTCACTCAGGGCAAACGGCTCGACTGTCCAGGCCTGGCCATc                                                                  <  2:353359/135‑1 (MQ=255)
                                                                        gcgAGAAACGCGGGCACAGCAGCCAACACCGAGGCGATAAAGCCTTTCATGTCGGCCGTGTCGCGGCCATACAGGCGGCCGTCACGCTCACTCAGGGCAAACGGCTCGACTGTCCAGGCCTGGCCATCCACCg                                                             <  4:5033/133‑1 (MQ=255)
                                                                         cgAGAAACGCGGGCACAGCAGCCAACACCGAGGCGATAAAGCCATTCATGTCGGCCGTGTCGCGGCCATAGAGGCGGCCGTGACGGTCACTCAGGGCAAACGGCTCGACTTTCCCTGCCTGGCCCTCCACCGCca                                                          >  8:220973/1‑135 (MQ=255)
                                                                          gagaAACGCGGGCACAGCAGCCAACACCGAGGCGATAAAGCCTTTCATGTCGGCCGTGTCGCGGCCATACAGGCGGCCGTCACGCTCACTCAGGGCAAACGGCTCGACTGTCCAGGCCTGGCCATCCACCGGcac                                                         >  4:248543/1‑135 (MQ=255)
                                                                           agaAACGCGGGTACAGCAGCCAACACCGAGGCTATAAAGCCTTTCATGTCGGCCGTGTCGCGGCCATACAGGCGGCCGTCACGCTCACTCAGGGCAAACGGCTCGACTGTCCAGGCCTGGCCATCCACCGGcacc                                                        <  3:47267/135‑1 (MQ=255)
                                                                           agaAACGCGGGCACAGCAGCCAACACCGAGGCTATAAAGCCTTTCATGTCGGCCGTGTCGCGGCCATACAGGCGGCCGTCACGCTCACTCAGGGCAAACGGCTCGACTGTCCAGGCCTGGCCATCCACCGGcacc                                                        <  3:90982/135‑1 (MQ=255)
                                                                           agaAACGCGGGCACAGCAGCCAACACCGAGGCGATAAAGCCTTTCATGTCGGCCGTGTCGCGGCCATACAGGCGGCCGTCACGCTCACTCAGGGCAAACGGCTCGACTGTCCAGGCCTGGCCATCCACCGGcacc                                                        <  4:205763/135‑1 (MQ=255)
                                                                           agaAACGCGGGCACAGCAGACAACACCGAGGCGATAAAGCCTTTCATGTCGGCCGTGTCGCGGCCATACAGGCCGCCGTCCCGCTCACTCAGGGCAAACGGCTCGACAGTCCACGCCAGGCCCTCCACCGGcacc                                                        >  7:465658/1‑135 (MQ=255)
                                                                               ccgcgGCCACCGCACCCAACCCCGCGGCCATAAAGCCTTCAATGTCGGCGGTGCCGGGGCCATACCGGCGGCCGTCACGCTCACGCAGGGCAAACGGCTCGACTGTCCAGGCCTGGCCATCCACCGGCACCACAt                                                    <  7:213779/134‑1 (MQ=255)
                                                                               aCGCGGGCACAGCAGCCAACACCGAGGCGATAAAGCCTTTCATGTCGGCCGTGTCGCGGCCATACAGGCGGCCGTCACGCTCACTCAGGGCAAACGGCTCGACTGTCCAGGCCTGGCCATCCACCGGCACCACAt                                                    <  8:166292/135‑1 (MQ=255)
                                                                               aCGCGGGCACAGCAGCCAACACCGAGGCGATAAAGCCTTTCATGTCGGCCGTGTCGCGGCCATACAGGCGGCCGTCACGCTCACTCAGGGCAAACGGCTCGACTGTCCAGGCCTGGCCATCCACCGGCACCACAt                                                    <  8:228238/135‑1 (MQ=255)
                                                                               aCGCGGGCACAGCAGCCAACACCGAGGCGATAAAGCCTTTCATGTCGGCCGTGTCGCGGCCATACAGGCGGCCGTCACGCTCACTCAGGGCAAACGGCTCGACTGTCCAGGCCTGGCCATCCACCGGCACCACAt                                                    >  4:444129/1‑135 (MQ=255)
                                                                               aCGCGGGCACAGCAGCCAACACCGAGGCGATAAAGCCTTTCATGTCGGCCGTGTCGCGGCCATACAGGCGGCCGTCACGCTCACTCAGGGCAAACGGCTCGACTGTCCAGGCCTGGCCATCCACCGGCACCACAt                                                    <  5:221867/135‑1 (MQ=255)
                                                                               aCGCGGGCACAGCAGCCAACACCGAGGCGATAAAGCCTTTCATGTCGGCCGTGTCGCGGCCATACAGGCGGCCGTCACGCTCACTCAGGGCAAACGGCTCGACTGTCCAGGCCTGGCCATCCACCGGCACCACAt                                                    <  4:134844/135‑1 (MQ=255)
                                                                                    ggCACAGCAGCCAACACCGAGGCGATAAAGCCTTTCATGTCGGCCGTGTCGCGGCCATACAGGCGGCCGTCACGCTCACTCAGGGCAAACGGCTCGACTGTCCAGGCCTGGCCATCCACCGGCACCACATCGGTa                                               <  8:284946/135‑1 (MQ=255)
                                                                                    ggCACAGCAGCCAACACCGAGGCGATAAAGCCTTTCAGGTCGGCCGGGTCGCGGCCATACAGGCGGCCGTCACGCTCACTCAGGGCAAACGGCTCGACTGTCCAGGCCTGGCCATCCACCGGCACCACATCGGTa                                               <  7:208144/135‑1 (MQ=255)
                                                                                         agcagcCAACACCGAGGCGATAAAGCCTTTCATGTCGGCCGTGTCGCGGCCATACAGGCGGCCGTCACGCTCACTCAGGGCAAACGGCTCGACTGTCCAGGCCTGGCCATCCACCGGCACCACATCGGTATGccc                                          <  3:236482/135‑1 (MQ=255)
                                                                                               cAACACCGAGGCGATAAAGCCTTTCATGTCGGCCGTGTCGCGGCCATACAGGCGGCCGTCACGCTCACTCAGGGCAAACGGCTCGACTGTCCAGGCCTGGCCATCCACCGGCACCACATCGGTATGCCCAGACAg                                    <  8:261593/135‑1 (MQ=255)
                                                                                               cAACACCGAGGCGATAAAGCCTTTCATGTCGGCCGTGTCGCGGCCATACAGGCGGCCGTCACGCTCACTCAGGGCAAACGGCTCGACTGTCCAGGCCTGGCCATCCACCGGCACCACATCGGTATGCCCAGACAg                                    <  4:178943/135‑1 (MQ=255)
                                                                                                 acacCGAGGCGATAAAGCCTTTCATGTCGGCCGTGTCGCGGCCATACAGGCGGCCGTCACGCTCACTCAGTGCAAACGGCTCGACTGTCCAGGCCTGGCCATCCACCGGCACCACATCGGTATGCCCAGACAGca                                  <  5:234249/135‑1 (MQ=255)
                                                                                                   acCGAGGCGATAAAGCCTTTCATGTCGGCCGTGGCGCGGCAATACAGGCGGCCGTCACGCTCAATCAGGGCAAACGGCTCGACTGTCCAGGCCTGGCCATCCACCGGCACCACATCGGTATGCCCAGACAGcacc                                <  5:371612/135‑1 (MQ=255)
                                                                                                    ccGAGGCTATAACGCCTTTCAGGTCGGCCGTGTCGCGGCCATACAGGCGGCCGTCACGCGCACTCAGGGCAAACGGCTCGACTGTCCAGGCCTGGCCATCCACCGGCACCACATCGGTATGCCCAGACAGcacca                               <  8:469954/135‑1 (MQ=255)
                                                                                                    ccGAGGCGATAAAGCCTTTCATGTCGGCCGTGTCGCGGCCATACAGGCGGCCGTCACGCTCACTCAGGGCAAACGGCTCGACTGTCCAGGCCTGGCCATCCACCGGCACCACATCGGTATGCCCAGACAGcacca                               <  6:392080/135‑1 (MQ=255)
                                                                                                    ccGAGGCGATAAAGCCTTTCATGTCGGACGTGTCGCGGCCATACAGGCGGCCGTCACGCTCACTCAGGGCAAACGGCTCGACTGTCCAGGCCTGGCCATCCACCGGCACCACATCGGTATGCCCAGACAGcacca                               <  2:147699/135‑1 (MQ=255)
                                                                                                        ggCGATAAAGCCTTTCATGTCGGCCGTGTCGCGGCCATACAGGCGTCCGTCACGCTCACTCAGGGCAAACGGCTCGACTGTCCAGGCCTGGCCATCCACCGGCACCACATCGGTATGCCCAGACAGcaccacacc                           <  4:154628/135‑1 (MQ=255)
                                                                                                        ggCGATAAAGCCTTTCATGTCGGCCGTGTCGCGGCCATACAGGCGGCCGTCACGCTCACTCAGGGCAAACGGCTCGACTGTCCAGGCCTGGCCATCCACCGGCACCACATCGGTATGCCCAGACAGcaccacacc                           >  8:154524/1‑135 (MQ=255)
                                                                                                          cGATAAAGCCTTTCATGTCGGCCGTGTCGCGGCCATACAGGCGGCCGTCACGCTCACTCAGGGCAAACGGCTCGACTGTCCAGGCCTGGCCATCCACCGGCACCACATCGGTATGCCCAGACAGcaccacacca                          >  4:241098/1‑134 (MQ=255)
                                                                                                          cGATAAAGCCTATCATGTCGGCCGTGTCGCGGCCATACAGGCGGCCGTCACGCTCACTCAGGGCAAACGGCTCGACTGTCCAGGCCTGGCCATCCACCGCCACCACATCGGTATGCCCAGACAGccccccacca                          >  8:433079/1‑134 (MQ=255)
                                                                                                           gttAAAGCGTTTCATGGCGGCCGGGGCGGGGCCATAGAGGCGGCCGTCACGCTCACTCAGGGCAAACGGCTCGACTGTCCAGGCCTGGCCATCCACCGGCACCACATCGGTATGCCCAGACAGCACCACaccacc                        <  4:308505/133‑1 (MQ=255)
                                                                                                           gaaacaGCCTTTCAGGGCGGCCGTGGCGCGGCCATACCGGCGGCCGTCACGCTCACTCAGGGCAAACGGCTCGACTGTCCAGGCCTGGCCATCCACCGGCACCACATCGGTATGCCCAGACAGCACCACaccacc                        <  6:314657/130‑1 (MQ=255)
                                                                                                                aGCCTTTCATGTCGGCCGTGTCGCGGCCATACAGGCGGCCGTCACGCTCACTCAGGGCAAACGGCTCGACTGTCCAGGCCTGGCCATCCACCGGCACCACATCGGTATGCCCAGACAGCACCACACCACCAACa                    <  7:179662/134‑1 (MQ=255)
                                                                                                                 gCCTTTCATGTCGGCCGTGTCGCGGCCATACAGGCGGCCGTCACGCTCACTCAGGGCAAACGGCTCGACTGTCCAGGCCTGGCCATCCACCGGCACCACATCGGTATGCCCAGACAGCACCACACCACCAACATc                  <  3:444129/135‑1 (MQ=255)
                                                                                                                    tttCATGTCGGCCGTGTCGCGGCCATACAGGCGGCCGTCACGCTCACTCAGGGCAAACGGCTCGACTGTCCAGGCCTGGCCATCCACCGGCACCACATCGGTATGCCCAGACAGCACCACACCACCAACATCCCt               <  7:154524/135‑1 (MQ=255)
                                                                                                                        aTGTCGGCCGTGTCGCGGCCATACAGGCGGCCGTCAc                                                                                                             <  7:40833/37‑1 (MQ=255)
                                                                                                                        aTGTCGGCCGTGTCGCGGCCATACAGGCGGCCGTCAc                                                                                                             >  8:40833/1‑37 (MQ=255)
                                                                                                                         tGTCGGCCGTGTCGCGGCCATACAGGCGGCCGTCACGCTCACTCAGGGCAAACGGCTCGACTGTCCAGGCCTGGCCATCCACCGGCACCACATCGGTATGCCCAGACAGCACCACACCACCAACATCCCTGGGGc          <  3:241098/135‑1 (MQ=255)
                                                                                                                         tGTCGGCCGTGTCGCGGCCATACAGGCGGCCGTCACGCTCACTCAGGGCAAACGGCTCGACTGTCCAGGCCTGGCCATCCACCGGCACCACATCGGTATGCCCAGACAGCACCACACCACCAACATCCCTGGGGc          >  1:310390/1‑135 (MQ=255)
                                                                                                                           ccGGCCGTGGCGCGGCCATACCGGCGGCCGTCACGCTCACTCAGGGCAAACGGCTCGACTGTCCAGGCCTGGCCATCCACCGGCACCACATCGGTATGCCCAGACAGCACCACACCACCAACATCCCTGGGGc          <  5:418227/132‑1 (MQ=255)
                                                                                                                                 gtgtCGCGGCCATACAGGCGGCCGTCACGCTCACTCAGGGCAAACGGCTCGACTGTCCAGGCCTGGCCATCCACCGGCACCACATCGGTATGCCCAGACAGCACCACACCACCAACATCCCTGGGGCCGACAGTg  >  8:448233/1‑135 (MQ=255)
                                                                                                                                    |                                                                                                                                   
GAGCGCACGCCCAAACAACCGACTTCTTCGTCATAGGAAAACGCCAGGTGTACCGGCATGCGCAACGGCTGCGCGAGAAACGCGGGCACAGCAGCCAACACCGAGGCGATAAAGCCTTTCATGTCGGCCGTGCCGCGGCCATACAGGCGGCCGTCACGCTCACTCAGGGCAAACGGCTCGACTGTCCAGGCCTGGCCATCCACCGGCACCACATCGGTATGCCCAGACAGCACCACACCACCAACATCCCTGGGGCCGACAGTG  >  ACB122/4045651‑4045914

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑