Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	ACB122	1,155,638	G→A	R118H (CGC→CAC)	oprB_1 →	Porin B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	ACB122	1,155,638	0	G	A	96.8%	206.1 / ‑1.8	63	R118H (CGC→CAC)	oprB_1	Porin B
Reads supporting (aligned to +/- strand):  ref base G (1/1);  new base A (25/36);  total (26/37)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.04e-01

ACGCTACAGCGACCAGTTCGCCCTCGGCGCGCATCTGGACTTGCAGAAGATACTGGGCTGGCATGATGCCGAGTTCAAGCTGGCAATCACCGAGCGAAGCGGTCGCAACCTGTCCAACGACCGCATCAGCGACCCGCGCGCCGGGCAGTTCAGCTCGGTGCAGGAGGTGTGGGGCCGTGGCCAGACCTGGCGCCTGACCCAGATGTGGATCAAGCAGAAGTACTTCGACGGCGCGCTGGACGTGAAATTTGG  >  ACB122/1155516‑1155767                                                                                                                           |                                                                                                                                  aCGCTACAGCGACCAGTTCGCCCTCGGCGCGCATCTGGACTTGCAGAAGATACTGGGCTGGCATGATGCCGAGTTCAAGCTGGCAATCACCGAGCGAAGCGGTCGCAACCTGTCCAACGACCACATCAGCGACCcgc                                                                                                                     <  8:92531/137‑1 (MQ=255) aCGCTACAGCGACCAGTTCGCCCTCGGCGCGCATCTGGACTTGCAGAAGATACTGGGCTGGCATGATGCCGAGTTCAAGCTGGCAATCACCGAGCGAAGCGGTCGCAACCTGTCCAACGACCACATCAGCGACCcgc                                                                                                                     >  4:252700/1‑137 (MQ=255)   gCTACAGCGACCAGTTCGCCCTCGGCGCGCATCTGGACTTGCAGAAGATACTGGGCTGGCATGATGCCGAGTTCAAGCTGGCAATCACCGAGCGAAGCGGTCGCAACCTGTCCAACGACCACATCAGCGACCcgcgc                                                                                                                   >  5:33653/1‑137 (MQ=255)   gCTACAGCGACCAGTTCGCCCTCGGCGCGCATCTGGACTTGCAGAAGATACTGGGCTGGCATGATGCCGAGTTCAAGCTGGCAATCACCGAGCGAAGCGGTCGCAACCTGTCCAACGACCACATCAGCGACCcgcgc                                                                                                                   >  6:142157/1‑137 (MQ=255)   gCTACAGCGACCAGTTCGCCCTCGGCGCGCATCTGGACTTGCAGAAGATACTGGGCTGGCATGATGCCGAGTTCAAGCTGGCAATCACCGAGCGAAGCGGTCGCAACCTGTCCAACGACCACATCAGCGACCcgcgc                                                                                                                   >  2:43358/1‑137 (MQ=255)    cTACAGCGACCAGTTCGCCCTCGGCGCGCATCTGGACTTGCAGAAGATACTGGGCTGGCATGATGCCGAGTTCAAGCTGGCAATCACCGAGCGAAGCGGTCGCAACCTGTCCAACGACCACATCAGCGACCcgcgcg                                                                                                                  <  1:164868/137‑1 (MQ=255)      ccAGCGACCAGTTCGCCCTCGGCGCGCATCTGGACTTCCAGAAGATACTGGGCTGTCATGTTGCCGAGTTCAAGCTGGCAATCACCGAGCGAAGCGGTCGCAACCTGTCCAACGACCACATCAGCGACCCGCGCGcc                                                                                                                <  6:233542/136‑1 (MQ=255)      aCAGCGACCAGTTCGCCCTCGGCGCGCATCTGGACTTGCAGAAGATACTGGGCTGGCATGATGCCGAGTTCAAGCTGGCAATCACCGAGCGAAGCGGTCGCAACCTGTCCAACGACCACATCAGCGACCCGCGCGcc                                                                                                                <  6:162005/137‑1 (MQ=255)      aCAGCGACCAGTTCGCCCTCGGCGCGCATCTGGACTTGCAGAAGATACTGGGCTGGCATGATGCCGAGTTCAAGCTGGCAATCACCGAGCGAAGCGGTCGCAACCTGTCCAACGACCACATCAGCGACCCGCGCGcc                                                                                                                <  6:120898/137‑1 (MQ=255)      aCAGCGACCAGTTCGCCCTCGGCGCGCATCTGGACTTGCAGAAGATACTGGGCTGGCATGATGCCGAGTTCAAGCTGGCAATCACCGAGCGAAGCGGTCGCAACCTGTCCAACGACCACATCAGCGACCCGCGCGcc                                                                                                                <  5:271038/137‑1 (MQ=255)      aCAGCGACCAGTTCGCCCTCGGCGCGCATCTGGACTTGCAGAAGATACTGGGCTGGCATGATGCCGAGTTCAAGCTGGCAATCACCGAGCGAAGCGGTCGCAACCTGTCCAACGACCACATCAGCGACCCGCGCGcc                                                                                                                <  3:269768/137‑1 (MQ=255)      aCAGCGACCAGTGCGCCCTCGGCGCGCATCTGGACTTGCAGAAGATACTGGGCTGGCATGATGCCGAGTTCAATCTGGCAATCACCGAGCGAAGCGGTCGCAACCTGTCCAACGACCACATCAGCGACCCGCGCGcc                                                                                                                <  4:22679/137‑1 (MQ=255)             ccAGTTCGCCCTCGGCGCGCATCTGGACTTGCAGAAGATACTGGGCTGGCATGATGCCGAGTTCAAGCTGGCAATCACCGCGCGAAGCGGTCGCAACCTGTCCAACGACCACATCAGCGACCCGCGCGCCGGGCAGt                                                                                                         >  8:307812/1‑137 (MQ=255)               aGTTCGCCCTCGGCGCGCATCTGGACTTGCAGAAGATACTGGGCTGGCATGATGCCGAGTTCAAGCTGGCAATCACCGAGCGAAGCGGTCGCAACCTGTCCAACGACCACATCAGCGACCCGCGCGCCGGGCAGTTc                                                                                                       >  8:357238/1‑137 (MQ=255)               aGTTCGCCCTCGGCGCGCATCTGGACTTGCAGAAGATACTGGGCTGGCATGATGCCGAGTTCAAGCTGGCAATCACCGAGCGAAGCGGTCGCAACCTGTCCAACGACCACATCAGCGACCCGCGCGCCGGGCAGTTc                                                                                                       >  5:39169/1‑137 (MQ=255)                gTTCGCCCTCGGCGCGCTTCTGGACTGTCAGAAGATACTGGGCTGGCATTATGCCGAGTTCAAGCTGGCAATCACCGAGCGAAGCGGTCGCAACCTGTCCAACGACCACATCAGCGACCCGCGCGCCGGGCAGTTCa                                                                                                      <  2:321695/137‑1 (MQ=255)                gTTCGCCCTCGGCGCGCATCTGGACTTGCAGAAGATACTGGGCTGGCATGATGCCGAGTTCAAGCTGGCAATCACCGAGCGAAGCGGTCGCAACCTGTCCAACGACCACATCAGCGACCCGCGCGCCGGGCAGTTCa                                                                                                      <  2:251577/137‑1 (MQ=255)                 ttCGCCCTCGGCGCGCATCTGGATTTGCAGAAGATACTGGGCTGGCATGATGCCGAGTTCAAGCTGGCAATCACCGAGCGAAGCGGTCGCAACCTGTCCAACGACCACATCAGCGACCCGCGCGCCGGGCAGTTCAg                                                                                                     <  7:91504/137‑1 (MQ=255)                 ttCGCCCTCGGCGCGCATCTGGACTTGCAGAAGATACTGGGCTGGCATGATGCCGAGTTCAAGCTGGCAATCACCGAGCGAAGCGGTCGCAACCTGTCCAACGACCACATCAGCGACCCGCGCGCCGGGCAGTTCAg                                                                                                     >  5:233542/1‑137 (MQ=255)                  tCGCCCTCGGCGCGCATCTGGACTTGCAGAAGATACTGGGCTGGCATGATGCCGAGTTCAAGCTGGCAATCACCGAGCGAAGCGGTCGCAACCTGTCCAACGACCACATCAGCGACCCGCGCGCCGGGCAGTTCAGc                                                                                                    >  8:100184/1‑137 (MQ=255)                  tCGCCCTCGGCGCGCATCTGGACTTGCAGAAGATACTGGGCTGGCATGATGCCGAGTTCAAGCTGGCAATCACCGAGCGAAGCGGTCGCAACCTGTCCAACGACCACATCAGCGACCCGCGCGCCGGGCAGTTCAGc                                                                                                    >  6:208549/1‑137 (MQ=255)                      ccTCGGCGCGCATCTGGACTTGCAGAAGATACTGGGCTGGCATGATGCCGAGTTCAAGCTGGCAATCACCGAGCGAAGCGGTCGCAACCTGTCCAACGACCACATCAGCGACCCGCGCGCCGGGCAGTTCAGCTCgg                                                                                                <  2:86708/137‑1 (MQ=255)                       cTCGGCGCGCATCTGGACTTGCAGAAGATACTGGGCTGGCATGATGCCGAGTTCAAGCTGGCAATCACCGAGCGAAGCGGTCGCAACCTGTCCAACGACCACATCAGCGACCCGCGCGCCGGGCAGTTCAGCTCGGt                                                                                               <  6:115514/137‑1 (MQ=255)                        tCGGCGCGCATCTGGACTTGCAGAAGATACTGGGCTGGCATGATGCCGAGTTCAAGCTGGCAATCACCGAGCGAAGCGGTCGCAACCTGTCCAACGACCACATCAGCGACCCGCGCGCCGGGCAGTTCAGCTCGGTg                                                                                              <  5:142157/137‑1 (MQ=255)                         ccgcgcgaATCTGTACTTGCAGAAGATACTGGGCTGGCATGATGCCGAGTTCAAGCTGGCAATCACCTAGCGAAGCGGTCGCAACCTGTACAACGACCACATCAGCGACCCGCGCGCCGGGCAGTTCAGCTCTGTGc                                                                                             <  1:296086/135‑1 (MQ=255)                         cGGCGCGCATCTGGACTTGCAGAAGATACTGGGCTGGCATGATGCCGAGTTCAAGCTGGCAATCACCGAGCGAAGCGGTCGCAACCTGTCCAACGACCACATCAGCGACCCGCGCGCCGGGCAGTTCAGCTCGGTGc                                                                                             <  5:174421/137‑1 (MQ=255)                         cGGCGCGCATCTGGACTTGCAGAAGATACTGGGCTGGCATGATGCCGAGTTCAAGCTGGCAATCACCGAGCGAAGCGGTCGCAACCTGTCCAACGACCACATCAGCGACCCGCGCGCCGGGCAGTTCAGCTCGGTGc                                                                                             <  7:357238/137‑1 (MQ=255)                         cGGCGCGCATCTGGACTTGCAGAAGATACTGGGCTGGCATGATGCCGAGTTCAAGCTGGCAATCACCGAGCGAAGCGGTCGCAACCTGTCCAACGACCACATCAGCGACCCGCGCGCCGGGCAGTTCAGCTCGGTGc                                                                                             <  7:307812/137‑1 (MQ=255)                                cATCTGGACTTGCAGAAGATACTGGGCTGGCATGATGCCGAGTTCAAGCTGGCAATCACCGACCGAAGCGGTCGCAACCTGTCCAACGACGACATCAGCGAACCGCGCGCCGGGCAGTTCAGCTCGGTGCAGGAGgt                                                                                      <  6:162442/137‑1 (MQ=255)                                   cTGGACTTGCAGAAGATACTGGGCTGGCATGATGCCGAGTTCAAGCTGGCAATCACCGAGCGAAGCGGTCGCAACCTGTCCAACGACCACATCAGCGACCCGCGCGCCGGGCAGTTCAGCTCGGTGCAGGAGGtgtg                                                                                   >  7:340771/1‑137 (MQ=255)                                          tGCAGAAGATACTGGGCTGGCATGATGCCGAGTTCAAGCTGGCAATCACCGAGCGAAGCGGTCGCAACCTGTCCAACGACCACATCAGCGACCCGCGCGCCGGGCAGTTCAGCTCGGTGCAGGAGGTGTGGGGCCGt                                                                            <  7:100184/137‑1 (MQ=255)                                              gaagaTACTGGGCTGGCATGATGCCGAGTTCAAGCTGGCAATCACCGAGCGAAGCGGTCGCAACCTGTCCAACGACCACATCAGCGACCCGCGCGCCGGGCAGTTCAg                                                                                                     <  5:160570/108‑1 (MQ=255)                                              gaagaTACTGGGCTGGCATGATGCCGAGTTCAAGCTGGCAATCACCGAGCGAAGCGGTCGCAACCTGTCCAACGACCACATCAGCGACCCGCGCGCCGGGCAGTTCAg                                                                                                     >  6:160570/1‑108 (MQ=255)                                                       gggCTGGCATGATGCCGAGTTCAAGCTGGCAATCACCGAGCGAAGCGGTCGCAACCTGTCCAACGACCGCATCAGCGACCCGCGCGCCGGGCAGTTCAGCTCGGTGCAGGAGGTGTGGGGCCGTGGCCAGACCTGgc                                                               <  5:319575/137‑1 (MQ=255)                                                       gggCTGGCATGATGCCGAGTTCAAGCTGGCAATCACCGAGCGAAGCGGTCGCAACCTGTCCAACGACCACATCAGCGACCCGCGCGCCGGGCAGTTCAGCTCGGTGCAGGAGGTGTGGGGCCGTGGCCAGACCTGgc                                                               <  3:252700/137‑1 (MQ=255)                                                            ggCATGATGCCGAGTTCAAGCTGGCAATCACCGAGCGAAGCGGTCGCAACCTGTCCAACGACCACATCAGCGACCCGCGCGCCGGGCAGTTCAGCTCGGTGCAGGAGGTGTGGGGCCGTGGCCAGACCTGGCGCCTg                                                          <  6:212504/137‑1 (MQ=255)                                                            ggCATGATGCCGAGTTCAAGCTGGCAATCACCGAGCGAAGCGGTCGCAACCTGTCCAACGACCACATCAGCGACCCGCGCGCCGGGCAGTTCAGCTCGGTGCAGGAGGTGTGGGGCCGTGGCCAGACCTGGCGCCTg                                                          <  2:142815/137‑1 (MQ=255)                                                            ggCATGATGCCGAGTTCAAGCTGGCAATCACCGAGCGAAGCGGTCGCAACCTGTCCAACGACCACATCAGCGACCCGCGCGCCGGGCAGTTCAGCTCGGTGCAGGAGGTGTGGGGCCGTGGCCAGACCTGGCGCCTg                                                          <  6:85986/137‑1 (MQ=255)                                                            ggCATGATGCCGAGTTCAAGCTGGCAATCACCGAGCGAAGCGGTCGCAACCTGTCCAACGACCACATCAGCGACCCGCGCGCCGGGCAGTTCAGCTCGGTGCAGGAGGTGTGGGGCCGTGGCCAGACCTGGCGCCTg                                                          <  4:136652/137‑1 (MQ=255)                                                              cATGATGCCGAGTTCAAGCTGGCAATCACCGAGCGAAGCGGTCGCAACCTGTCCAACGACCACATCAGCGACCCGCGCGCCGGGCAGTTCAGCTCGGTGCAGGAGGTGTGGGGCCGTGGCCAGCCCTGGCGCCTGAc                                                        >  8:14596/1‑137 (MQ=255)                                                              cATGATGCCGAGTTCAAGCTGGCAATCACCGAGCGAAGCGGTCGCAACCTGTCCAACGACCACATCAGCGACCCGCGCGCCGGGCAGTTCAGCTCGGTGCAGGAGGTGTGGGGCCGTGGCCAGACCTGGCGCCTGAc                                                        >  5:362611/1‑137 (MQ=255)                                                                tgatgCCGAGTTCAAGCTGGCAATCACCGAGCGAAGCGGTCGCAACCTGTCCAACGACCACATCAGCGACCCGCGCGCCGGGCAGTTCAGCTCGGTGCAGGAGGTGTGGGGCCg                                                                             >  8:137957/1‑114 (MQ=255)                                                                tgatgCCGAGTTCAAGCTGGCAATCACCGAGCGAAGCGGTCGCAACCTGTCCAACGACCACATCAGCGACCCGCGCGCCGGGCAGTTCAGCTCGGTGCAGGAGGTGTGGGGCCg                                                                             <  7:137957/114‑1 (MQ=255)                                                                tgatgCCGAGTTCAAGCTGGCAATCACCGAGCGAAGCGGTCGCAACCTGTCCAACGACCACATCAGCGACCCGCGCGCCGGGCAGTTCAGCTCGGTGCAGGAGGTGTGGGGCCGTGGCCAGACCTGGCGCCTGAccc                                                      >  4:329471/1‑137 (MQ=255)                                                                 gatgCCGAGTTCAAGCTGGCAATCACCGAGCGAAGCGGTCGCAACCTGTCCAACGACCACATCAGCGACCCGCGCGCCGGGCAGTTCAGCTCGGTGCAGGAGGTGTGGGGCCGTGGCCAGACCTGGCGCCTGACCCa                                                     <  5:324423/137‑1 (MQ=255)                                                                     ccGAGTTCAAGCTGGCAATCACCGAGCGAAGCGGTCGCAACCTGTCCAACGACCACATCAGCGACCCGCGCGCCGGGCAGTTCAGCTCGGTGCAGGAGGTGTGGGGCCGTGGCCAGACCTGGCGCCTGACCCAGAtg                                                 >  4:38749/1‑137 (MQ=255)                                                                      cGAGTTCAAGCTGGCAATCAACGAGCGAAGCGGTCGCAACCTGTCCAACGACCACATCAGCGACCCGCGCGCCGGGCAGTTCAGCTCGGTGCAGGAGGTGTGGGGCCGTGGCCAGACCTGGCGCCTGACCCAGAtgt                                                <  2:363694/137‑1 (MQ=255)                                                                      cGAGTTCAAGCTGGCAAGCCCCGCGCGACGCGGTCGCAACCTGTCCAACGACCACATCAGCGACCCGCGCGCCGGGCAGTTCAGCTCGGTGCAGGAGGTGTGGGGCCGTGGCCAGACCTGGCGCCTGACCCAGAtgt                                                <  2:157022/137‑1 (MQ=255)                                                                       gAGTTCAAGCTGGCAATCACCGAGCGAAGCGGTCGCAACCTGTCCAACGACCACATCAGCGACCCGCGCGCCGGGCAGTTCAGCTCGGTGCAGGAGGTGTGGGGCCGTGGCCAGACCTGGCGCCTGACCCAGAtgtg                                               <  7:259705/137‑1 (MQ=255)                                                                                cTGGCAATCACCGAGCGAAGCGGTCGCAACCTGTCCAACGACCGCATCAGCGACCCGCGCGCCGGGCAGTTCAGCTCGGTGCAGGAGGTGTGGGGCCGTGGCCAGACCTGGCGCCTGACCCAGATGTGGATCAAGCa                                      >  1:10523/1‑137 (MQ=255)                                                                                cTGGCAATCACCGAGCGAAGCGGTCGCAACCTGTCCAACGACCACATCAGCGACCCGCGCGCCGGGCAGTTCAg                                                                                                     >  7:130089/1‑74 (MQ=255)                                                                                cTGGCAATCACCGAGCGAAGCGGTCGCAACCTGTCCAACGACCACATCAGCGACCCGCGCGCCGGGCAGTTCAg                                                                                                     <  8:130089/74‑1 (MQ=255)                                                                                       tCACCGAGCGAAGCGGTCGCAACCTGTCCAACGACCACATCAGCGACCCGCGCGCCGGGCAGTTCAg                                                                                                     <  7:185251/67‑1 (MQ=255)                                                                                       tCACCGAGCGAAGCGGTCGCAACCTGTCCAACGACCACATCAGCGACCCGCGCGCCGGGCAGTTCAg                                                                                                     >  8:185251/1‑67 (MQ=255)                                                                                           cGAGCGAAGCGGTCGCAACCTGTCCAACGACCACATCAGCGACCCGCGCGCCGGGCAGTTCAGCTCGGTGCAGGAGGTGGGGGGCCGTGGCCAGACCTGGCGCCTGACCCAGATGTGGATCAAGCAGAAGTACTTCg                           <  2:330463/137‑1 (MQ=255)                                                                                                gAAGCGGTCGCAACCTGTCCAACGACCACATCAGCGACCCGCGCGCCGGGCAGTTCAGCTCGGTGCAGGAGGTGTGGGGCCGTGGCCAGACCTGGTGCCTGACCCAGATGTGGATCAAGCAGAAGTACTTCGACGgc                      >  5:300337/1‑137 (MQ=255)                                                                                                    cGGTCGCAACCTGTCCAACGACCACATCAGCGACCCGCGCGCCGGGCAGTTCAGCTCGGTGCAGGAGGTGTGGGGCCGTGGCCAGCCCTGGCGCCTGACCCAGATGTGGATCAAGCAGAAGTACTTCGACGgcgcgc                  >  6:131903/1‑137 (MQ=255)                                                                                                        cGCAACCTGTCCAACGACCACATCAGCGACCCGCGCGCCGGGCAGTTCAGCTCGGTGCAGGAGGTGTGGGGCCGTGGCCAGACCTGGCGCCTGACCCAGATGTGGATCAAGCAGAAGTACTTCGACGGCGCGCTggc              >  2:61473/1‑136 (MQ=255)                                                                                                                    aaCGACCACATCAGCGACCCGCGCGCCGGGCAGTTCAGCTCGGTGCAGGAGGTGTGGGGCCGTGGCCAGACCTGGCGCCTGACCCAGATGTGGATCAAGCAg                                     <  6:200540/102‑1 (MQ=255)                                                                                                                    aaCGACCACATCAGCGACCCGCGCGCCGGGCAGTTCAGCTCGGTGCAGGAGGTGTGGGGCCGTGGCCAGACCTGGCGCCTGACCCAGATGTGGATCAAGCAg                                     >  5:200540/1‑102 (MQ=255)                                                                                                                    aaCGACCACATCAGCGACCCGCGCGCCGGGCAGTTCAGCTCGGTGCAGGAGGTGTGGGGCCGTGGCCAGACCTGGCGCCTGACCCAGATGTGGATCAAGCAGAAGTACTTCGACGGCGCGCTGGACGTGAAATTTgg  >  3:151306/1‑137 (MQ=255)                                                                                                                    aaCGACCACATCAGCGACCCGCGCGCCGGGCAGTTCAGCTCGGTGCAGGAGGTGTGGGGCCGTGGCCAGACCTGGCGCCTGACCCAGATGTGGATCAAGCAGAAGTACATCGACGGCGCGCTGGACGTGAAATTTgg  >  5:142457/1‑137 (MQ=255)                                                                                                                    aaCGACCACATCAGCGACCCGCGCGCCGGGCAGTTCAGCTCGGTGCAGGAGGGGTGGGGCCGTGGCCAGACCTGGCGCCTGACCCAGATGTGGATCAAGCAGAAGTACTTCGACGGCGCGCTGGACGTGAAATTTgg  <  1:343872/137‑1 (MQ=255)                                                                                                                           |                                                                                                                                  ACGCTACAGCGACCAGTTCGCCCTCGGCGCGCATCTGGACTTGCAGAAGATACTGGGCTGGCATGATGCCGAGTTCAAGCTGGCAATCACCGAGCGAAGCGGTCGCAACCTGTCCAACGACCGCATCAGCGACCCGCGCGCCGGGCAGTTCAGCTCGGTGCAGGAGGTGTGGGGCCGTGGCCAGACCTGGCGCCTGACCCAGATGTGGATCAAGCAGAAGTACTTCGACGGCGCGCTGGACGTGAAATTTGG  >  ACB122/1155516‑1155767

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑