| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | ACB122 | 1,835,872 | T→C | S660G (AGC→GGC) | barA ← | Signal transduction histidine‑protein kinaseBarA |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | ACB122 | 1,835,872 | 0 | T | C | 75.0% | 10.3 / 5.3 | 18 | S660G (AGC→GGC) | barA | Signal transduction histidine‑protein kinaseBarA |
| Reads supporting (aligned to +/- strand): ref base T (3/1); new base C (0/12); total (3/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.14e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.95e-02 | |||||||||||
CATAACCATTGTTGACGGCCAGTACCTCGGCACCCAGGTCTTCGAGCAGGGTTTTTACCAGCAGCAGGTTGGCGGCATTGTCGTCGACGCAGAGTATTTTCGGTCGTCGCTG‑GCCGTTGGGGTTGCTCCCCTCGGCCAGCGGACGGCGAGGTTGCACCAGCTCCAGCAAGAGGCGCCGCAGCTTGCGGGTGCAGGTCGGCTTGGACAGCAACTGGCCATGGCCGTTGGGCAGGTACGGGTGGTACAGCGCCTGCTCGGTGGT > ACB122/1835746‑1836007 | cATAACCATTGTTGACGGCCAGTACCTCGGCACCCAGGTCTTCGAGCAGGGTTTTTACCAGCAGCAGGTTGGCGGCATTGTCGTCGACGCAGAGTATTTTCGGTCGTGGCGGTGGCGTTTGGGTGGGTcccccgggg > 2:196239/1‑132 (MQ=255) aTAACCATTGTTGACGGCCAGTACCTCGGCACCCAGGTCTTCGAGCAGGGTTTTTACCAGCAGCAGGTTGGCGGCATTGTCGTCGACGCAGAGTATTTTCGGTCGTCGCTG‑GGCGGTGGGGTTGGTCCCCACCGccc > 5:1059/1‑136 (MQ=255) ccAGTACCTCGGCACCCAGGTCTTCGAGCAGGTTTTTTACCAGCAGCAGGTTGGCGGCTTTGTCGTCGACGCAGAGTATTTTCGGTCGGCGCTG‑GCCGTTGGGGTGGCCCCCCTCGGCCAGCGGACGGCGAGGTTGc < 7:266554/137‑1 (MQ=255) tgcGAGCCGGGTTTTACCCAGCAGCAGGTGGGCGCCATGGGCGTCCCCGCAGTGTATTTGCGGGCGGCGCGG‑GCCGGTGGGGGGCCCCCCCGCGCCCAGCGGACGGCGAGGTTGCACCAGCTCCAGCAAGAGGcgcc < 2:204359/135‑1 (MQ=255) aaccaccaccTGGTGGGCGGATTTGGCGGCGACGCAGTGTTTTTTCGGGCGGCGGTG‑GCGTTGGGGGTGGCGCCCCTCGGCCAGCGGACGGCGAGGTTGCACCAGCTCCAGCAAGAGGCGCCGCAGCTTGCGGGTGc < 8:19427/136‑1 (MQ=255) cccgcagGTGGGCGGCTTTGGCGTCGCCGCTGTTTTTTTTGGGGCGGGGCGG‑GCGTTGGGGGTGGCCCCCCCCGGCCCGCGGACGGCGAGGTTGCACCAGCTCCAGCAAGAGGCGCCGCAGCTTGCGGGTGCAGGTc < 4:110537/134‑1 (MQ=255) cagccgGTGGGCGGCATTGGCGTCGACGCAGCTTATTTTCGGCCGGCGGTG‑GCCGTTGGGGTGCCCCCCCGCGGCCAGGGGACGGCGAGGGTGCACCAGCTCCAGCAAGAGGCGCCGCAGCTTGCGGGTGCAGGTCg < 6:336253/137‑1 (MQ=255) gtggtcgACGCAGTTTATTTGGGGGGGTCGGTG‑GCGGTTGGGGTGGCCCCCCGCGGCCTGCGGACGGCGAGGTTGCACCAGCTCCAGCAAGAGGCGCCGCAGCTTGCGGGTGCAGGTCGGCTTGGACAGCAACTGGc < 3:252678/134‑1 (MQ=255) gtcgtcGACGCAGAGTATTTTCGGGCGGCGCGG‑GCCGTTGGGGTGGCGCCCCTCGGCCAGCGGACGGCGAGGTTGCACCAGCTCCAGCAAGAGGCGCCGCAGCTTGCGGGTGCAGGTCGGCTTGGACAGCAACTGGc < 2:264112/137‑1 (MQ=255) cgccGCTGAGTTTTTTCGGGCGGGGGTG‑GCGGTTGGGGTGGCCCCCCGCGGCCCGCGGACGGCGAGGTTGCACCAGCTCCAGCAAGAGGCGCCGCAGCTTGCGGGTGCAGGTCGGCTTGGACAGCAACTGGCCATgg < 4:102901/134‑1 (MQ=255) cAGAGTTTTTTCGGGCGTCGCTT‑TCCGTTGGGGGTGCCCCCCGCGGCCAGCGGACGGCGAGGTTGCACCAGCTCCAGCAAGAGGCGCCGCAGCTTGCGGGTGCAGGTCGGCTTGGACAGCAACTGGCCATGGCCGtt < 6:1059/137‑1 (MQ=255) aaTTTTCGGGCGGCGCTG‑GCCGTGGGGGTTGACCCCCTCGGCCAGCGGACGGCGAGGTTGCACCATCTCCAGCAAGAGGCGCCGCAGCTTGCGGGTGCAGGTCGGCTTGGACAGCAACTGGCCATGGCCGTTGGGCa < 8:100705/136‑1 (MQ=255) tCGGTCGTCGCTG‑ACCGTTGGGGTTGCTCCCCTCGGCCAGCGGACGGCGAGGTTGCACCAGCTCCAGCAAGAGGCGCCGCAGCTTGCGGGTGCAGGTCGGCTTGGACAGCAACTGGCCATGGCCGTTGGGCAGGTAc < 8:120756/137‑1 (MQ=255) ggggTCGGCGCGG‑GCGGTGGGGGTGCCCCCCCGGGCCCCGCGGACGGCGAGGTTGCACCAGCTCCAGCAAGAGGCGCCGCAGCTTGCGGGTGCAGGTCGGCTTGGACAGCAACTGGCCATGGCCGTTGGGCAGGTAc < 3:179015/135‑1 (MQ=255) gcGGTGGGCGCGG‑GCCGTTGGGGTGGCCCCCCGCGGCCGGCGGACGGCGAGGTTGCACCAGCTCCAGCAAGCGGCGCCGCAGCTTGCGGGTGCAGGTCGGCTTGGACAGCAACTGGCCATGGCCGTTGGGCAGGTat < 8:115893/136‑2 (MQ=255) gcGGGCGGCGCGG‑GCCGTTGGGGTGGCCCCCCTCCGCCAGCGCACGGCGAGGTTGCACCAGCTCCAGCAAGAGGCGCCGCAGCTTGCGGGTGCAGGTCGGCTTGGACAGCAACTGGCCATGGCCGTTGGGCAGGTAc < 6:279082/136‑1 (MQ=255) ggggGTGCCCCCGGCGCCCCGCGGACGGCGAGGGTGCACCAGCTCCAGCAAGAGGCGCCGCAGCGTGCGGGTGCAGGTCGGCTTGGACAGCAACTGGCCATGGCCGTTGGGCAGGTACGGGTGGTACAGCGCCTGCt < 8:179407/137‑1 (MQ=255) cTCCCCTCGGCCAGCGGACGGCGAGGTTGCACCAGCTCCAGCAAGAGGCGCCGCAGCTTGCGGGTGCAGGTCGGCTTGGACAGCAACTGGCCATGGCCGTTGGGCAGGTACGGGTGGTACAGCGCCTGCTCggtggt > 5:273261/1‑137 (MQ=255) | CATAACCATTGTTGACGGCCAGTACCTCGGCACCCAGGTCTTCGAGCAGGGTTTTTACCAGCAGCAGGTTGGCGGCATTGTCGTCGACGCAGAGTATTTTCGGTCGTCGCTG‑GCCGTTGGGGTTGCTCCCCTCGGCCAGCGGACGGCGAGGTTGCACCAGCTCCAGCAAGAGGCGCCGCAGCTTGCGGGTGCAGGTCGGCTTGGACAGCAACTGGCCATGGCCGTTGGGCAGGTACGGGTGGTACAGCGCCTGCTCGGTGGT > ACB122/1835746‑1836007 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |