Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	ACB122	3,066,184	T→G	L278R (CTG→CGG)	rcsC_6 →	Sensor histidine kinase RcsC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	ACB122	3,066,184	0	T	G	100.0%	269.4 / NA	79	L278R (CTG→CGG)	rcsC_6	Sensor histidine kinase RcsC
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (41/38);  total (41/38)

TGGAGCGTACCCACCAGGCCCTGGAGGGCGCGGAAGACCTGCTGACCGACTTGCTGGACATTTCCCGGCTCGACCAGGCGGCGGTAAAGCCGGATGTGGCCGTGTACCGCCTGGACGAGCTGTTCGCGCCGCTGGTCTCGGAGTTCAGCCCGGTGGCCGAGGCGGCGGGCCTGAAGCTGCATGCACGCATAGCCGACTACGCCATCAGCACTGACCTGCGGTTGCTCACACGCATCCTGCGCAACTTCCTCAGCAACGCTTG  >  ACB122/3066052‑3066313                                                                                                                                     |                                                                                                                                  tggAGCGTACCCACCAGGCCCTGGAGGGCGCGGAAGACCTGCTGACCGACTTGCTGGACATTTCCCGGCTCGACCAGGCGGCGGTAAAGCCGGATGTGGCCGTGTACCGCCTGGACGAGCTGTTCGCGCCGCGGGtc                                                                                                                               >  1:273612/1‑137 (MQ=255)      cGTACCCACCAGGCCCTGGAGGGCGCGGAAGACCTGCTGACCGACTTGCTGGACATTTCCCGGCTCGACCAGGCGGCGGTAAAGCCGGATGTGGCCGTGTACCGCCTGGACGAGCTGTTCGCGCCGCGGGTCTCGGa                                                                                                                          <  3:249209/137‑1 (MQ=255)       gTACCCACCAGGCCCTGGAGGGCGCGGAAGACCTGCTGACCGACTTGCTGGACATTTCCCGGCTCGACCAGGCGGCGGTAAAGCCGGATGTGGCCGTGTACCGCCTGGACGAGCTGTTCGCGCCGCGGGTCTCGGAg                                                                                                                         >  1:129118/1‑137 (MQ=255)        tACCCACCAGGCCCTGGAGGGCGCGGAAGACCTGCTGACCGACTTGCTGGACATTTCCCGGCTCGACCAGGCGGCGGTAAAGCCGGATGTGGCCGTGTACCGCCTGGACGAGCTGTTCGCGCCGCGGGTCTCGGAGt                                                                                                                        >  7:95718/1‑137 (MQ=255)         aCCCACCAGGCCCTGGAGGGCGCGGAAGACCTGCTGACCGACTTGCTGGACATTTCCCGGCTCGACCAGGCGGCGGTAAAGCCGGATGTGGCCGTGTACCGCCTGGACGAGCTGTTCGCGCCGCGGGTCTCGGAGtt                                                                                                                       >  8:361685/1‑137 (MQ=255)         aCCCACCAGGCCCTGGAGGGCGCGGAAGACCTGCTGACCGACTTGCTGGACATTTCCCGGCTCGACCAGGCGGCGGTAAAGCCGGATGTGGCCGTGTACCGCCTGGACGAGCGGTTCGCGCCGCGGGGCTCGGAGtt                                                                                                                       >  3:26804/1‑137 (MQ=255)           ccaccaGGCCCTGGAGGGCGCGGAAGACCTGCTGACCGACTTGCTGGACATTTCCCGGCTCGACCAGGCGGCGGTAAAGCCGGATGTGGCCGTGTACCGCCTGGACGAGCTGTTCGCGCCGCGGGTCTCGGAGTTCa                                                                                                                     >  8:289391/1‑137 (MQ=255)           ccaccaGGCCCTGGAGGGCGCGGAAGACCTGCTGACCGACTTGCTGGACATTTCCCGGCTCGACCAGGCGGCGGTAAAGCCGGATGTGGCCGTGTACCGCCTGGACGAGCTGTTCGCGCCGCGGGTCTCGGAGTTCa                                                                                                                     >  4:343120/1‑137 (MQ=255)             accaGGCCCTGGAGGGCGCGGAAGACCTGCTGACCGACTTGCTGGACATTTCCCGGCTCGACCAGGCGGCGGTAAAGCCGGATGTGGCCGTGTACCGCCTGGACGAGCTGTTCGCGCCGCGGGTCTCGGAGTTCAGc                                                                                                                   >  8:274369/1‑137 (MQ=255)                  gCCCTGGAGGGCGCGGAAGACCTGCTGACCGACTTCCTGGACATTTCCCGGCTCGACCAGGCGGCGGTAAAGCCGGATGTGGCCGTGTACCGCCTGGACGAGCTGTTCGCGCCGCGGGTCTCGGAGTTCAGCCCGGt                                                                                                              >  4:37341/1‑137 (MQ=255)                   cccTGGTGGGCGCGGAAGACGTGCTGACCGACTTGCTCGTCATTTCCCGGCGCGACCAGGCTGCGGTAAAGCCGGATGTGGCCGTGTACGGCCTGGACGAGCTGTGCGCGCCGCGGGTCTCGGAGTTCAGCCCGGTg                                                                                                             <  3:314254/137‑1 (MQ=255)                     cTGGAGGGCGCGGAAGACCTGCTGACCGACTTGCTGGACATTTCCCGGCTCGACCAGGCGGCGGTAAAGCCGGATGTGGCCGTGTACCGCCTGGACGAGCTGTTCGCGCCGCGGGTCTCGGAGTTCAGACCGGTggg                                                                                                           >  1:258935/1‑136 (MQ=255)                     cTGGAGGGCGCGGAAGACCTGCTGACCGACTTGCTGGACATTTCCCGGCTCGACCAGGCGGCGGTAAAGCCGGATGTGGCCGTGTACCGCCGGGTCGAGCTGTTCGCGCCGCGGGTCTCGGAGTTCAGCCCGGTggg                                                                                                           >  2:176946/1‑136 (MQ=255)                      tGGAGGGCGCGGAAGACCTGCTGACCGACTTGCTGGACATTTCCCGGCTCGACCAGGCGGCGGTAAAGCCGGATGTGGCCGTGTACCGCCTGGACGAGCTGTTCGCGCCGCGGGTCTCGGAGTTCAGCCCGGTGGcc                                                                                                          >  3:108769/1‑137 (MQ=255)                         aGGGCGCGGAAGACCTGCTGACCGACTTGCTGGACATTTCCCGGCTCGACCAGGCGGCGGTAAAGCCGGATGTGGCCGTGTACCCCCTCGACGCGCTGTTCGCGCCGCGGGTCTCGGAGTTCAGCCCGGTGGCCGAg                                                                                                       >  6:33184/1‑137 (MQ=255)                           ggCGCGGAAGACCTGCTGACCGACTTGCTGGACATTTCCCGGCTCGACCAGGCGGCGGTAAAGCCGGATGTGGCCGTGTACCGCCTGGACGAGCTGTTCGCGCCGCGGGTCTCGGAGTTCAGCCCGGTGGCCGAggc                                                                                                     <  2:273612/137‑1 (MQ=255)                           ggCGCGGAAGACCTGCTGACCGACTTGCTGGACATTTCCCGGCTCGACCAGGCGGCGGTAAAGCCGGATGTGGCCGTGTACCGCCTGGACGAGCTGTGCGCGCCGCGGGTCTCGGAGTTCAGCCCGGTGGCCGAggc                                                                                                     <  4:635/137‑1 (MQ=255)                              gcgGAAGACCTGCTGACCGACTTGCTGGACATTTCCCGGCTCGACCAGGCGGCGGTAAAGCCGGATGTGGCCGTGTACCGCCTGGACGAGCTGTTCGCGCCGCGGGTCTCGGAGTTCAGCCCGGTGGCCGAggcggc                                                                                                  >  3:184763/1‑137 (MQ=255)                                ggAAGACCTGCTGACCGACTTGCTGGACATTTCCCGGCTCGACCAGGCGGCGGTAAAGCCGGATGTGGCCGTGTACCGCCTGGACGAGCTGTTCGCGCCGCGGGTCTCGGAGTTCAGCCCGGTGGCCGAGGcggcgg                                                                                                >  5:195248/1‑137 (MQ=255)                                ggAAGACCTGCTGACCGACTTGCTGGACATTTCCCGGCTCGACCAGGCGGCGGTAAAGCCGGATGTGGCCGTGTACCGCCTGGACGAGCTGTTCGCGCCGCGGGTCTCGGAGTTCAGCCCGGTGGCCGAGGcggcgg                                                                                                >  5:286655/1‑137 (MQ=255)                                ggAAGACCTGCTGACCGACTTGCTGGACATTTCCCGGCTCGACCAGGCGGCGGTAAAGCCGGATGTGGCCGTGTACCGCCTGGACGAGCTGTTCGCGCCGCGGGTCTCGGAGTTCAGCCCGGTGGCCGAGGcggcgg                                                                                                >  3:302740/1‑137 (MQ=255)                                ggAAGACCTGCTGACCGACTTGCTGGACATTTCCCGGCTCGACCAGGCGGCGGTAAAGCCGGATGTGGCCGTGTACCGCCTGGACGAGCTGTTCGCGCCGCGGGTCTCGGAGTTCAGCCCGGTGGCCGAGGcggcgg                                                                                                >  6:200992/1‑137 (MQ=255)                                ggAAGACCTGCTGACCGACTTGCTGGACATTTCCCGGCTCGACCAGGCGGCGGTAAAGCCGGATGTGGCCGTGTACCGCCTGGACGAGCTGTTCGCGCCGCGGGTCTCGGAGTTCAGCCCGGTGGCCGAGGcggcgg                                                                                                >  3:57680/1‑137 (MQ=255)                                          ctgACCGACTTGCTGGACATTTCCCGGCTCGACCAGGCGGCGGTAAAGCCGGATGTGGCCGTGTACCGCCTGGACGAGCTGTTCGCGCCGCGGGTCTCGGAGTTCAGCCCGGTGGCCGAGGCGGCGGGCCTGAAGCt                                                                                      >  1:72037/1‑137 (MQ=255)                                            gACCGACTTGCTGGACATTTCCCGGCTCGACCAGGCGGCGGTAAAGCCGGATGTGGCCGTGTACCGCCTGGACGAGCTGTTCGCGCCGCGGGTCTCGGAGTTCAGCCCGGTGGCCGAGGCGGCGGGCCTGAAGCtgc                                                                                    >  3:187157/1‑137 (MQ=255)                                            gACCGACTTGCTGGACATTTCCCGGCTCGACCAGGCGGCGGTAAAGCCGGATGTGGCCGTGTACCGCCTGGACGAGCTGTTCGCGCCGCGGGTCTCGGAGTTCAGCCCGGTGGCCGAGGCGGCGGGCCTGAAGCtgc                                                                                    <  2:288980/137‑1 (MQ=255)                                            gACCGACTTGCTGGACATTTCCCGGCTCGACCAGGCGGCGGTAAAGCCGGATGTGGCCGTGTAACGCCTGGACGAGCTGTTAGCGCAGCGGGTCTCGGAGTTCAGCCCGGTGGCCGAGGCGGCGGGCCTGAAGCtgc                                                                                    >  3:347567/1‑137 (MQ=255)                                                     gCTGGACATTTCCCGGCTCGACCAGGCGGCGGTAAAGCCGGATGTGGCCGTGTACCGCCTGGACGAGCTGTTCGCGCCGCGGGTCTCGGAGTTCAGCCCGGTGGCCGAGGCGGCGGGCCTGAAGCTGCATGCACGCa                                                                           <  4:293641/137‑1 (MQ=255)                                                           cATTTCCCGGCTCGACCAGGCGGCGGTAAAGCCGGATGTGGCCGTGTACCGCCTGGACGAGCGGTTCGCGCCGCGGGTCTCGGAGTTCAGCCCGGTGGCCGAGGCGGCGGGCCTGAAGCTGCATGCACGCATAGCCg                                                                     <  8:194332/137‑1 (MQ=255)                                                                cccGGCTCGACCAGGCGGCGGTAAAGCCGGATGTGGCCGTGTACCGCCTGGACGCGCTGTTCGCGCCGCGGGTCTCGGAGTTCAGCCCGGTGGCCGAGGCGGCGGGCCTGAAGCTGCATGCACGCATAGCCGACTAc                                                                >  1:357942/1‑137 (MQ=255)                                                                       cGACCAGGCGGCGGTAAAGCCGGATGTGGCCGTGTACCGCCTGGACGAGCTGTTCGCGCCGCGGGTCTCGGAGTTCAGCCCGGTGGCCGAGGCGGCGGGCCTGAAGCTGCGTGCACGCATAGCCGACTACGCCATCa                                                         <  3:151130/137‑1 (MQ=255)                                                                          ccAGGCGGCGGTAAAGCCGGATGTGGCCGTGTACCGCCTGGACGAGCTGTTCGCGCCGCGGGTCTCGGAGTTCAGCCCGGTGGCCGAGGCGGCGGGCCTGAAGCTGCATGCACGCATAGCCGACTACGCCATCAGCa                                                      <  1:243289/137‑1 (MQ=255)                                                                           cAGGCGGCGGTAAAGCCGGATGTGGCCGTGTACCCCCTGGACGAGCTGTTCGCGCCGCGGGTCTCGGAGTTCAGCCCGGTGGCCGAGGCGGCGGGCCTGAAGCTGCATGCACGCATAGCCGACTACGCCATcaccac                                                     >  4:12592/1‑137 (MQ=255)                                                                                  cggTAAAGCCGGATGTGGCCGTGTACCGCCTGGACGAGCTGTTCGCGCCGCGGGTCTCGGAGTTCAGCCCGGTGGCCGAGGCGGCGGGCCTGAAGCTGCATGCACGCATAGCCGACTACGCCATCAGCACTGACCTg                                              <  5:248244/137‑1 (MQ=255)                                                                                  cggTAAAGCCGGAGGTGGCCGTGTACCGCCTGGACGAGCTGTTCGCGCCGCGGGTCTCGGAGGTCAGCCCGGTGGCCGAGGCGGCGGGCCTGAAGCTGCATGCACGCATAGCCGACTACGCCATCAGCACTGACCTg                                              <  2:336785/137‑1 (MQ=255)                                                                                     tAAAGCCGGATGTGGCCGTGTACCGCCTGGACGAGCTGTTCGCGCCGCGGGTCTCGGAGTTCAGCCCGGTGGCCGAGGCGGCGGGCCTGAAGCTGCATGCACGCATAGCCGACTACGCCATCAGCACTGACCTGCgg                                           >  4:308020/1‑137 (MQ=255)                                                                                     tAAAGCCGGAGGTGGCGGGGTACCGCCTGGAGGAGCTGTTCGCGCCGCGGGTCTCGGAGTTCAGCCCGGTGGCCGAGGCGGCGGGCCTGAAGCTGCATGCACGCATAGCCGACTACGCCATCAGCACTGACCTGCgg                                           <  5:33184/137‑1 (MQ=255)                                                                                       aaGGCGGATGTGGCCGTGGACCGCCTGGACGAGCTGTTCGCGCCGCGGGTCTCGGAGTTCAGCCCGGTGGCCGAGTCGGGGGGCCTGAAGCTGCATGCACGCATAGCCGACTACGCCATCAGCACTGACCTGCGGtt                                         <  7:319986/137‑1 (MQ=255)                                                                                       aaGCCGGATGTGGCCGTGTACCGCCTGGACGAGCTGTTCGCGCCGCGGGTCTCGGAGTTCAGCCCGGTGGCCGAGGCGGCGGGCCTGAAGCTGCATGCACGCATAGCCGACTACGCCATCAGCACTGACCTGCGGtt                                         <  3:37341/137‑1 (MQ=255)                                                                                       aaGCCGGATGTGGCCGTGTACCGCCTGGACGAGCTGTTCGCGCCGCGGGTCTCGGAGTTCAGCCCGGTGGCCGAGGCGGCGGGCCTGAAGCTGCATGCACGCATAGCCGACTACGCCATCAGCACTGACCTGCGGtt                                         <  5:333712/137‑1 (MQ=255)                                                                                       aaGCCGGATGTGGCCGTGTACCGCCTGGACGAGCTGTTCGCGCCGCGGGTCTCGGAGTTCAGCCCGGTGGCCGAGGCGGCGGGCCTGAAGCTGCATGCACGCATAGCCGACTACGCCATCAGCACTGACCTGCGGtt                                         <  2:72037/137‑1 (MQ=255)                                                                                       aaGCCGGATGTGGCCGTGTACCGCCTGGACGAGCTGTTCGCGCCGCGGGTCTCGGAGTTCAGCCCGGTGGCCGAGGCGGCGGGCCTGAAGCTGCATGCACGCATAGCCGACTACGCCATCAGCACTGACCTGCGGtt                                         <  7:289391/137‑1 (MQ=255)                                                                                       aaGCCGGATGTGGCCGTGTACCGCCTGGACGAGCTGTTCGCGCCGCGGGTCTCGGAGTTCAGCCCGGTGGCCGAGGCGGCGGGCCTGAAGCTGCATGCACGCATAGCCGACTACGCCATCAGCACTGACCTGCGGtt                                         <  7:196460/137‑1 (MQ=255)                                                                                       aaGCCGGATGTGGCCGTGTACCGCCTGGACGAGCTGTTCGCGCCGCGGGTCTCGGAGTTCAGCCCGGTGGCCGAGGCGGCGGGCCTGAAGCTGCATGCACGCATAGCCGACTACGCCATCAGCACTGACCTGCGGtt                                         <  5:353108/137‑1 (MQ=255)                                                                                          ccGGATGTGGCCGTGTACCGCCTGGACGAGCTGTTCGCGCCGCGGGTCTCGGAGTTCAGCCCGGTGGCCGAGGCGGCGGGCCTGAAGCTGCATGCACGCATAGCCGACTACGCCATCAGCACTGACCTGCGGTTGCt                                      >  3:254214/1‑137 (MQ=255)                                                                                            ggATGTGGCCGTGTACCGCCTGGACGAGCTGTTCGCGCCGCGGGTCTCGGAGTTCAGCCCGGTGGCCGAGGCGGCGGGCCTGAAGCTGCATGCACGCATAGCCGACTACGCCATCAGCACTGACCTGCGGTTGCTca                                    <  3:50068/137‑1 (MQ=255)                                                                                            ggATGTGGCCGTGTACCGCCTGGACGAGCTGTTCGCGCCGCGGGTCTCGGAGTTCAGCCCGGTGGCCGAGGCGGCGGGCCTGAAGCTGCATGCACGCATAGCCGACTACGCCATCAGCACTGACCTGCGGTTGCTca                                    <  4:108769/137‑1 (MQ=255)                                                                                                  ggCCGTGTACCGCCTGGACGAGCTGTTCGCGCCGCGGGTCTCGGAGTTCAGCCCGGTGGCCGAgg                                                                                                      <  1:174359/65‑1 (MQ=255)                                                                                                  ggCCGTGTACCGCCTGGACGAGCTGTTCGCGCCGCGGGTCTCGGAGTTCAGCCCGGTGGCCGAgg                                                                                                      >  2:174359/1‑65 (MQ=255)                                                                                                      gtgtACCGCCTGGACGAGCTGTTCGCGCCGCGGGTCTCGGAGTTCAGCCCGGTGGCCGAGGCGGCGGGCCTGAAGCTGCATGCACGCATAGCCGACTACGCCATCAGCACTGACCTGCGGTTGCTCACACGCATCCt                          >  5:323320/1‑137 (MQ=255)                                                                                                        gtACCGCCTGGACGAGCTGTTCGCGCCGCGGGTCTCGGAGTTCAGCCCGGTGGCCGAGGCGGCGGGCCTGAAGCTGCATGCACGCATAGCCGACTACGCCATCAGCACTGACCTGCGGTTGCTCACACGCATCCTgc                        <  8:148847/137‑1 (MQ=255)                                                                                                        gtACCGCCTGGACGAGCTGTTCGCGCCGCGGGTCTCGGAGTTCAGCCCGGTGGCCGAGGCGGCGGGCCTGAAGCTGCATGCACGCATAGCCGACTACGCCATCAGCACTGACCTGCGGTTGCTCACACGCATCCTgc                        <  1:148998/137‑1 (MQ=255)                                                                                                        gtACCGCCTGGACGAGCTGTTCGCGCCGCGGGTCTCGGAGTTCAGCCCGGTGGCCGAGGCGGCGGGCCTGAAGCTGCATGCACGCATAGCCGACTACGCCATCAGCACTGACCTGCGGTTGCTCACACGCATCCTgc                        <  8:95718/137‑1 (MQ=255)                                                                                                          aCCGCCTGGACGAGCTGTTCGCGCCGCGGGTCTCGGAGTTCAGCCCGGTGGCCGAGGCGGCGGGCCTGAAGCTGCATGCACGCATAGCCGACTACGCCATCAGCACTGACCTGCGGTTGCTCACACGCATCCTgcgc                      <  3:308020/137‑1 (MQ=255)                                                                                                          aCCGCCTGGACGAGCTGTTCGCGCCGCGGGTCTCGGAGTTCAGCCCGGTGGCCGAGGCGGCGGGCCTGAAGCTGCATGCACGCATAGCCGACTACGCCATCAGCACTGACCTGCGGTTGCTCACACGCATCCTgcgc                      <  7:274369/137‑1 (MQ=255)                                                                                                           ccGCCTGGACGAGCTGTTCGCGCCGCGGGTCTCGGAGTTCAGCCCGGTGGCCGAGGCGGCGGGCCTGAAGCTGCATGCACGCATAGCCGACTACGCCATCAGCACTGACCTGCGGTTGCTCACACGCATCCTGCGCa                     >  5:54206/1‑137 (MQ=255)                                                                                                                tGGACGATCTGTTCGCGCCGCGGGTCTCGGAGTTCAGCCCGGTGGCCGAGGCGGCGGGCCATAAGCTGCATTCCCGCAGAGCCGACTACGCCATAAGCCCTGACCTGCGGTTGCTCACACGCATCCTGCGCAACTTc                >  8:177678/1‑137 (MQ=255)                                                                                                                 ggACGAGCTGTTCGCGCCGCGGGTCTCGGAGTTCAGCCCGGTGGCCGAGGCGGCGGGCCTGa                                                                                          <  3:9673/62‑1 (MQ=255)                                                                                                                 ggACGAGCTGTTCGCGCCGCGGGTCTCGGAGTTCAGCCCGGTGGCCGAGGCGGCGGGCCTGa                                                                                          >  4:9673/1‑62 (MQ=255)                                                                                                                 ggACGAGCTGTTCGCGCCGCGGGTCTCGGAGTTCAGCCCGGTGGCCGAGGCGGCGGGCCTGAAGCTGCATGCACGCATAGCCGACTACGCCATCCGCACTGACCTGCGGTAGCTCACACGCATCCTGCGCAACTTcc               >  2:116541/1‑137 (MQ=255)                                                                                                                 ggACGAGCTGTTCGCGCCGCGGGTCTCGGAGTTCAGCCCGGTGGCCGAGGCGGCGGGCCTGAAGCTGCATGCACGCATAGCCGACTACGCCATCAGCACTGACCTGCGGTTGCTCACACGCATCCTGCGCAACTTcc               >  1:146201/1‑137 (MQ=255)                                                                                                                 ggACGAGCTGTTCGCGCCGCGGGTCTCGGAGTTCAGCCCGGTGGCCGAGGCGGCGGGCCTGAAGCTGCATGCACGCATAGCCGACTACGCCATCAGCACTGACCTGCGGTTGCTCACACGCATCCTGCGCAACTTcc               >  3:166607/1‑137 (MQ=255)                                                                                                                 ggACGAGCTGTTCGCGCCGCGGGTCTCGGAGTTCAGCCCGGTGGCCGAGGCGGCGGGCCTGAAGCTGCATGCACGCATAGCCGACTACGCCATCAGCACTGACCTGCGGTTGCTCACACGCATCCTGCGCAACTTcc               >  2:80951/1‑137 (MQ=255)                                                                                                                 ggACGAGCTGTTCGCGCCGCGGGTCTCGGACTTAAGCCCGGTTGCCGAGGCGGCCGGCCTGAAGCTGCATGCCCGCATAGCCGACTACGCCATCAGCCCTGACCTGCGGTTGCTCACACCCATCCAGCGCAACTAcc               >  5:86898/1‑137 (MQ=255)                                                                                                                    cGAGCTGTTCGCGCCGCGGGTCTCGGAGTTCAGCCCGGTGGCCGAGGCGGCGGGCCTGAAg                                                                                        >  7:157542/1‑61 (MQ=255)                                                                                                                    cGAGCTGTTCGCGCCGCGGGTCTCGGAGTTCAGCCCGGTGGCCGAGGCGGCGGGCCTGAAg                                                                                        <  8:157542/61‑1 (MQ=255)                                                                                                                    cGAGCTGTTCGCGCCGCGGGTCTCGGAGTTCAGCCCGGTGGCCGAGGCGGCGGGCCTGAAGCTGCATGCACGCATAGCCGACTACGCCATCAGCACTGACCTGCGGTTGCTCACACGCATCCTGCGCAACTTCCTCa            >  7:283840/1‑137 (MQ=255)                                                                                                                         tGTTCGCGCCGCGGGTCTCGGAGTTCAGCCCGGTGGCCGAGGCGGCGGGCCTGAAGCTGCATGCACGCATAGCCGACTACGCCATCAGCACTGACCTGCGGTTGCTCACACGCATCCTGCGCAACTTCCTCAGCAAc       <  5:322264/137‑1 (MQ=255)                                                                                                                         tGTTCGCGCCGCGGGTCTCGGAGTTCAGCCCGGTGGCCGAGGCGGCGGGCCTGAAGCTGCATGCACGCATAGCCGACTACGCCATCAGCACTGACCTGCGGTTGCTCACACGCATCCTGCGCAACTTCCTCAGCAAc       <  4:57680/137‑1 (MQ=255)                                                                                                                         tGTTCGCGCCGCGGGTCTCGGAGTTCACCCCGGAGGCCGAGGCGCCGGGCCTGAAGCTGCAAGCACTCATAGCCGACTACGCCATCAGCACTGACATGCGGTTGCTCACACGCATCCTGCGCTACTTCCTCAGCAAc       >  3:185696/1‑137 (MQ=255)                                                                                                                           ttCGCGCCGCGGGTCTCGGAGTTCAGCCCGGTGGCCGAGGCGGCGGGCCTGAAGCTGCATGCACGCATAGCCGACTACGCCATCAGCACTGACCTGCGGtt                                         >  1:127628/1‑101 (MQ=255)                                                                                                                           ttCGCGCCGCGGGTCTCGGAGTTCAGCCCGGTGGCCGAGGCGGCGGGCCTGAAGCTGCATGCACGCATAGCCGACTACGCCATCAGCACTGACCTGCGGtt                                         <  2:127628/101‑1 (MQ=255)                                                                                                                           ttCGCGCCGCGGGTCTCGGAGTTCAGCCCGGTGGCCGAGGCGGCGGGCCTGAAGCTGCATGCACGCATAGCCGACTACGCCATCAGCACTGACCTGCGGTTGCTCACACGCATCCTGCGCAACTTCCTCAGCAACGc     <  6:286655/137‑1 (MQ=255)                                                                                                                           ttCGCGCCGCGGGTCTCGGAGTTCAGCCCGGTGGCCGAGGCGGCGGGCCTGAAGCTGCATGCACGCATAGCCGACTACGCCATCAGCACTGACCTGCGGTTGCTCACACGCATCCTGCGCAACTTCCTCAGCAACGc     <  2:210686/137‑1 (MQ=255)                                                                                                                           ttCGCGCCGCGGGTCTCGGAGTTCAGCCCGGTGGCCGAGGCGGCGGGCCTGAAGCTGCATGCACGCATAGCCGACTACGCCATCAGCACTGACCTGCGGTTGCTCACACGCATCCTGCGCAACTTCCTCAGCAACGc     <  2:129118/137‑1 (MQ=255)                                                                                                                           ttCGCGCCGCGGGTCTCGGAGTTCAGCCCGGTGGCCGAGGCGGCGGGCCTGAAGCTGCATGCACGCATAGCCGACTACGCCATCAGCACTGACCTGCGGTTGCTCACACGCATCCTGCGCAACTTCCTCAGCAACGc     >  3:232993/1‑137 (MQ=255)                                                                                                                            tCGCGCCGCGGGTCTCGGAGTTCAGCCGGGTGGGCGAGGCGGCGGGCCTGAAGCTGCATGCACTCATAGCCGACTACGCCATCAGGACTGACCTGCGGTTGCTCACACGCATCCTGCGCAACTTCCTCAGCAACGCt    <  2:327481/137‑1 (MQ=255)                                                                                                                              gcgcCGCGGGTCTCGGAGTTCAGCCCGGTGGCCGAGGCGGCGGGCCTGAAGCTGCATGCACGCATAGCCGACTACGCCATCAGCACTGACCTGCGGTTGCTCACACGCATCCTGCGCAACTTCCTCAGCAACGCTTg  <  2:357942/137‑1 (MQ=255)                                                                                                                              gcgcCGCGGGTCTCGGAGTTCAGCCCGGTGGCCGAGGCGGCGGGCCTGAAGCTGCATGCACGCATAGCCGACTACGCCATCAGCACTGACCTGCGGTTGCTCACACGCATCCTGCGCAACTTCCTCAGCAACGCTTg  <  7:361685/137‑1 (MQ=255)                                                                                                                                     |                                                                                                                                  TGGAGCGTACCCACCAGGCCCTGGAGGGCGCGGAAGACCTGCTGACCGACTTGCTGGACATTTCCCGGCTCGACCAGGCGGCGGTAAAGCCGGATGTGGCCGTGTACCGCCTGGACGAGCTGTTCGCGCCGCTGGTCTCGGAGTTCAGCCCGGTGGCCGAGGCGGCGGGCCTGAAGCTGCATGCACGCATAGCCGACTACGCCATCAGCACTGACCTGCGGTTGCTCACACGCATCCTGCGCAACTTCCTCAGCAACGCTTG  >  ACB122/3066052‑3066313

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑