| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | ACB122 | 4,647,581 | T→C | intergenic (+164/‑220) | CJ019_04390 → / → ndhC | hypothetical protein/NAD(P)H‑quinone oxidoreductase subunit 3,chloroplastic |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | ACB122 | 4,647,581 | 0 | T | C | 93.7% | 199.8 / 2.9 | 64 | intergenic (+164/‑220) | CJ019_04390/ndhC | hypothetical protein/NAD(P)H‑quinone oxidoreductase subunit 3,chloroplastic |
| Reads supporting (aligned to +/- strand): ref base T (3/1); new base C (33/26); total (37/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.29e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.64e-01 | |||||||||||
GGAATCGCCAGATGAACTTTACAAGGCGCAGAACAGCGAAATTCGAGCGCAGCAGTTCAAATGATATTAATTATCATTACAGGGTTTGCAATTCGTTACCCGCTGCAAGAAACATAATTAACAAAACCGCGCGTAATGCCCGAATTTCAAGGCTCTCAGCCAGTTACGAACGTTTTTTGTACTTAATCCTACGAATAAATTTGCGACAGAAATTTTACTTGCACCAGGTTTACCCATAAAATCAGCGCGATTGATCCGGCTGCGAC > ACB122/4647448‑4647713 | gtaaTCGCCAGATGAACTTTACAAGGCGCAGAACAGCGAAATTCGATCGCAGCAGTTCAAATTATATTAATTATCATTACAGGGTTTGCACTTCGTTACCCGCTGCAAGAAACATAATTCACAAAACCGCGCGCAAt < 7:339763/135‑1 (MQ=255) cGCCAGATGAACTTTACAAGGCGCAGAACAGCGAAATTCGAGCGCAGCAGTTCAAATGATATTAATTATCATTACAGGGTTTGCAATTCGTTACCCGCTGCAAGAAACATAATTAACAAAACCGCGCGCAATGCCCg > 5:373820/1‑137 (MQ=255) cAGATGAACTTTACAAGGCGCAGAACAGCTAAATTCGAGCGCAGCAGTTCAAATGATATTAATTATCATTACAGGGTTTGCAATTCGTTACCCGCTGCAAGAAACATAATTAACAAAACCGAGCGCAATGCCCGAAt > 4:337029/1‑137 (MQ=255) taTGAACTTTACAAGGCGCAGAACAGCGAAATTCGAGCGCAGCAGTTCAAATGATATTAATTATCATTACAGGGTTTGCAATTCGTTACCCGCTGCAAGAAACATAATTAACAAAACCGCGCGCAATGCCCGAAttt < 6:373021/136‑1 (MQ=255) gATGAACTTTACAAGGCGCAGAACAGCGAAATTCGAGCGCAGCAGTTCAAATGATATTAATTATCATTACAGGGTTTGCAATTCGTTACCCGCTGCAAGAAACATAATTAACAAAACCGCGCGCAATGCCCGAAttt < 2:323624/137‑1 (MQ=255) aGAACAGCGAAATTCGAGCGCAGCAGTTCAAATGATATTAATTATCATTACAGGGTTTGCAATTCGTTACCCGCTGCAAGAAACATAATTAACAAAACCGCGCGCAATGCCCGAATTTCAAGGCTCTCAGCCAGTTa > 7:47943/1‑137 (MQ=255) gAACAGCGAAATTCGAGCGCAGCAGTTCAAATGATATTAATTATCATTACAGGGTTTGCAATTCGTTACCCGCTGCAAGAAACATAATTAACAAAACCGCGCGCAATGCCCGAATTTCAAGGCTCTCAGCCAGTTAc > 5:219194/1‑137 (MQ=255) aaaTTCGAGCGCAGCATTTCAAATGATATTAATTATCATTACAGGGTTTGCAATTGGTTTCCAGCTGCAAGAAACATAATTAAGAAAACCGCTCGCAATGCACGAATTTCTAGGCTCTCAGCCAGTTACGTACGttt < 2:249020/137‑1 (MQ=255) aaaTTCGAGCGCAGCAGTTCAAATGATATTAATTATCATTACAGGGTTTGCAATTCGTTACCCGCTGCAAGAAACATAATTAACAAAACCGCGCGCAATGCCCGAATTTCAAGGCTCTCAGCCAGTTACGAACGttt < 4:355387/137‑1 (MQ=255) aaaTTCGAGCGCAGCAGTTCAAATGATATTAATTATCATTACAGGGTTTGCAATTCGTTACCCGCTGCAAGAAACATAATTAACAAAACCGCGCGCAATGCCCGAATTTCAAGGCTCTCAGCCAGTTACGAACGttt < 7:183353/137‑1 (MQ=255) aaTTCGAGCGCAGCAGTTCAAATGATATTAATTATCATTACAGTGTTTGCAATTCGTTACCCGCTGCAAGAAACATAATTAACAAAACCTCGCGAAATGCCCTAATTTCAAGGCTCACAGCCAGTGACGAACGtttt > 8:238235/1‑137 (MQ=255) aaTTCGAGCGCAGCAGTTCAAATGATATTAATTATCATTACAGGGTTTGCAATTCGTTACCCGCTGCAAGAAACATAATTAACAAAACCGCGCGTAATGCCCGAATTTCAAGGCTCTCAGCCAGTTACGAACGttt < 2:253160/136‑1 (MQ=255) aaTTCGAGCGCAGCAGTTCAAATGATATTAATTATCATTACAGGGTTTGCAATTCGTTACCCGCTGCAAGAAACATAATTAACAAAACCGCGCGTAATGCCCGAATTTCAAGGCTCTCAGCCAGTTACGAACGttt > 1:253160/1‑136 (MQ=255) aaTTCGAGCGCAGCAGTTCAAATGATATTAATTATCATTACAGGGTTTGCAATTCGTTACCCGCTGCAAGAAACATAATTAACAAAACCGCGCGCAATGCCCGAATTTCAAGGCTCTCAGCCAGTTACGAACGtttt > 4:195661/1‑137 (MQ=255) aaTTCGAGCGCAGCAGTTCAAATGATATTAATTATCATTACAGGGTTTGCAATTCGTTACCCGCTGCAAGAAACATAATTAACAAAACCGCGCGCAATGCCCGAATTTCAAGGCTCTCAGCCAGTTACGAACGtttt > 6:313198/1‑137 (MQ=255) gAGCGCAGCAGTTCAAATGATATTAATTATCATTACAGGGTTTGCAATTCGTTACCCGCTGCAAGAAACATAATTAACAAAACCGCGCGCAATGCCCGAATTTCAAGGCTCTCAGCCAGTTACGAACGTTTTTTGTa > 4:21147/1‑137 (MQ=255) gcagcaGTTCAAATGATATTAATTATCATTACAGGGTTTGCAATTCGTTACCCGCTGCAAGAAACATAATTAACAAAACCGCGCGCAATGCCCGAATTTCAAGGCTCTCAGCCAGTTACGAACGTTTTTTGTACTTa < 4:334676/137‑1 (MQ=255) ttCAAATGATATTAATTATCATTACAGGGTTTGCAATTCGTTACCCGCTGCAAGAAACATAATTAACAAAACCGCGCGCAATGCCCGAATTTCAAGGCTCTCAGCCAGTTACGAACGTTTTTTGTACTTAATCCTAc > 3:41482/1‑137 (MQ=255) aTGATATTAATTATCATTACAGGGTTTGCAATTCGTTACCCGCTGCAAGAAACATAATTAACAAAACCGCGCGCAATGCCCGAATTTCAAGGCTCTCAGCCAGTTACGAACGTTTTTTGTACTTAATCCTACGAATa > 2:220721/1‑137 (MQ=255) atatTAATTATCATTACAGGGTTTGCAATTCGTTACCCGCTGCAAGAAACATAATTAACAAAACCGCGCGCAATGCCCGAATTTCAAGGCTCTCAGCCAGTTACGAACGTTTTTTGTACTTAATCCTACGAATAAAt > 6:129140/1‑137 (MQ=255) aattaTCATTACAGGGTTTGCAATTCTTTACCCGATGAAAGAAACATAATTAACAAAACCTCGCGCAATGCCCGAATTTCAAGGCTCTCAGCCAGTTACGAACGTTTTTTGTACTTAATCATACTAATAAATATGCg > 4:281592/1‑137 (MQ=255) aattaTCATTACAGGGTTTGCAATTCGTTACCCGCTGCAAGAAACATAATTAACAAAACCGCGCGTAATGCCCGAATTTCAAGGCTCTCAGCCAGTTACGAACGTTTTTTGTACTTAATCCTACGAATAAATTTgag > 7:315348/1‑135 (MQ=255) aattaTCATTACAGGGTTTGCAATTCGTTACCCGCTGCAAGAAACATAATTAACAAAACCGCGCGCAATGCCCGAATTTCAAGGCTCTCAGCCAGTTACGAACGTTTTTTGTACTTAATCCTACGAATAAATTTGCg > 3:235128/1‑137 (MQ=255) aattaTCATTACAGGGTTTGCAATTCGTTACCCGCTGCAAGAAACATAATTAACAAAACCGCGCGCAATGCCCGAATTTCAAGGCTCTCAGCCAGTTACGAACGTTTTTTGTACTTAATCCTACGAATAAATTTGCg > 2:339147/1‑137 (MQ=255) attaTCATTACAGGGTTTGCAATTCGTTACCCGCTGCAAGAAACATAATTAACAAAACCGCGCGCAATGCCCGAATTTCAAGGCTCTCAGCCAGTTACGAACGTTTTTTGTACTTAATCCTACGAATAAATTTGCGa < 5:187530/137‑1 (MQ=255) attaTCATTACAGGGTTTGCAATTCGTTACCCGCTGCAAGAAACATAATTAACAAAACCGCGCGCAATGCCCGAATTTCAAGGCTCTCAGCCAGTTACGAACGTTTTTTGTACTTAATCCTACGAATAAATTTGCGa < 1:94526/137‑1 (MQ=255) aTCATTACAGGGTTTGCAATTCGTTACCCGCTGCAAGAAACATAATTAACAAAACCGCGCGCAATGCCCGAATTTCAAGGCTCTCAGCCAGTTACGAACGTTTTTTGTACTTAATCCTACGAATAAATTTGCGACAg > 8:54226/1‑137 (MQ=255) aaCAGGGTTTGCAATTAGTTACCCGCTGCAAGAAACATAATTAACAAAACAGCGCGCAAAGCCCGAATATCAAGGCTCACAGCCAGTAACGAACGTTTTTTGTACTTAAACCTACGAATAAATTTGCGACAAAAAtt > 5:153264/2‑137 (MQ=255) aCAGGGTTTGCAATTCGTTACCCGCTGCAAGAAACATAATTAACAAAACCGCGCGCAATGCCCGAATTTCAAGGCTCTCAGCCAGTTACGAACGTTTTTTGTACTTAATCCTACGAATAAATTTGCGACAGAAAttt < 7:54226/137‑1 (MQ=255) cAGGGTTTGCAATTCGTTACCCGCTGCAAGAAACATAATTAACAAAACCGCGCGCAATGCCCGAATTTCAAGGCTCTCAGCCAGTTACGAACGTTTTTTGTACTTAATCCTACGAATAAATTTGCGACAGAAAtttt < 8:305979/137‑1 (MQ=255) gCAATTCGTTACCCGCTGCAAGAAACATAATTAACAAAACCGCGCGCAATGCCCGAATTTCAAGGCTCTCAGCCAGTTACGAACGTTTTTTGTACTTAATCCTACGAATAAATTTGCGACAGAAATTTTACTTGCAc > 3:357975/1‑137 (MQ=255) cAATTCGTTACCCGCTGCAAGAAACATAATTAACAAAACCGCGCGCAATGCCCGAATTTCAAGGCTCTCAGCCAGTTACGAACGTTTTTTGTACTTAATCCTACGAATAAATTTGCGACAGAAATTTTACTTGCAcc > 8:168660/1‑137 (MQ=255) aaTTCGTTACCCGCTGCAAGAAACATAATTAACAAAACCGCGCGCAATGCCCGAATTTCAAGGCTCTCAGCCAGTTACGAACGTTTTTTGTACTTAATCCTACGAATAAATTTGCGACAGAAATTTTACTTGCACCa > 4:17968/1‑137 (MQ=255) aaTTCGTTACCCGCTGCAAGAAACATAATTAACAAAACCGCGCGCAATGCCCGAATTTCAAGGCTCTCAGCCAGTTACGAACGTTGTTTGTACTTAATCCTACGAATAAATTTGCGACAGAAATTTTACTTGCACCa > 6:68293/1‑137 (MQ=255) aTTCGTTACCCGCTGCAAGAAACATAATTAACAAAACCGCGCGCAATGCCCGAATTTCAAGGCTCTCAGCCAGTTACGAACGTTTTTTGTACTTAATCCTACGAATAAATTTGCGACAGAAATTTTAGTTGCACCAg > 2:193865/1‑137 (MQ=255) aTTCGTTACCCGCTGCAAGAAACATAATTAACAAAACCGCGCGCAATGCCCGAATTTCAAGGCTCTCAGCCAGTTACGAACGTTTTTTGTACTTAATCCTACGAATAAATTTGCGACAGAAATTTTACTTGCACCAg < 7:83008/137‑1 (MQ=255) aTTCGTTACCCGCTGCAAGAAACATAATTAACAAAACAGCGCGCAATGCCCGAATTTCAAGGCTCTCAGCCAGTTACGAACGTTTTTTGTACTTAATCCTACGAATAAATTTGCGACAGAAATTTTACTTGCACCAg < 7:338922/137‑1 (MQ=255) tCGTTACCCGCTGCAAGAAACATAATTAACAAAACCGCGCGCAATGCCCGAATTTCTAGGCTCTCAGCCAGTTACGAACGTTTTTTGTACTTAATCCTACGAATAAATTTGCGACAGAAATTTTACTTGCACCAGGt < 8:271185/137‑1 (MQ=255) tCGTTACCCGCTGCAAGAAACATAATTAACAAAACCGCGCGCAATGCCCGAATTTCAAGGCTCTCAGCCAGTTACGAACGTTTTTTGTACTTAATCCTACGAATAAATTTGCGACAGAAATTTTACTTGCACCAGGt < 4:240500/137‑1 (MQ=255) tCGTTACCCGCTGCAAGAAACATAATTAACAAAACCGCGCGCAATGCCCGAATTTCAAGGCTCTCAGCCAGTTACGAACGTTTTTTGTACTTAATCCTACGAATAAATTTGCGACAGAAATTTTACTTGCACCAGGt < 1:257989/137‑1 (MQ=255) tCGTTACCCGCTGCAAGAAACATAATTAACAAAACCGCGCGCAATGCCCGAATTTCAAGGCTCTCAGCCAGTTACGAACGTTTTTTGTACTTAATCCTACGAATAAATTTGCGACAGAAATTTTACTTGCACCAGGt < 5:47237/137‑1 (MQ=255) tCGTTACCCGCTGCAAGAAACATAATTAACAAAACCGCGCGCAATGCCCGAATTTCAAGGCTCTCAGCCAGTTACGAACGTTTTTTGTACTTAATCCTACGAATAAATTTGCGACAGAAATTTTACTTGCACCAGGt < 1:220721/137‑1 (MQ=255) tCGTTACCCGCTGCAAGAAACATAATTAACAAAACCGCGCGCAATGCCCGAATTTCAAGGCTCTCAGCCAGTTACGAACGTTTTTTGTACTTAATCCTACGAATAAATTTGCGACAGAAATTTTACTTGCACCAGGt < 5:263334/137‑1 (MQ=255) tCGTTACCCGCTGCAAGAAACATAATTAACAAAACCGCGCGCAATGCCCGAATTTCAAGGCTCTCAGCCAGTTACGAACGTCTTTTGTACTTAATCCTACGAATAAATTTGCGACAGAAATTTTACTTGCACCAGGt < 1:28903/137‑1 (MQ=255) ttACCCGCTGCAAGAAACATAATTAACAAAACCGCGCGCAATGCCCGAATTTCAAGGCTCTCAGCCAGTTACGAACGTTTTTTGTACTTAATCCTACGAATAAATTTGCGACAGAAATTTTACTTGCACCAg > 3:83090/1‑132 (MQ=255) ttACCCGCTGCAAGAAACATAATTAACAAAACCGCGCGCAATGCCCGAATTTCAAGGCTCTCAGCCAGTTACGAACGTTTTTTGTACTTAATCCTACGAATAAATTTGCGACAGAAATTTTACTTGCACCAg < 4:83090/132‑1 (MQ=255) ttACCCGCTGCAAGAAACATAATTAACAAAACCGCGCGCAATGCCCGAATTTCAAGGCTCTCAGCCAGTTACGAACGTTTTTTGTACTTAATCCTACGAATAAATTTGCGACAGAAATTTTACTTGCACCAGGTTTa > 6:334425/1‑137 (MQ=255) ttACCCGCTGCAAGAAACATAATTAACAAAACCGCGCGCAATGCCCGAATTTCAAGGCTCTCAGCCAGTTACGAACGTTTTTTGTACTTAATCCTACGAATAAATTTGCGACAGAAATTTTACTTGCACCAGGTTTa > 4:320499/1‑137 (MQ=255) tACCCGCTGCAAGAAACATAATTAACAAAACCGCGCGCAATGCCCGAATTTCAAGGCTCTCAGCCAGTTACGAACGTTTTTTGTACTTAATCCTACGAATAAATTTGCGACAGAAATTTTACTTGCACCAGGTTTAc < 5:313198/137‑1 (MQ=255) aCCCGCTGCAAGAAACATAATTAACAAAACCGCGTGCAATGCCCGAATTTCAAGGCTCTCAGCCAGTTACGAACGTTTTTTGTACTTAATCCTACGAATAAATTTGCGACAGAAATTTTACTTGCACCAGGTTTAcc > 7:247577/1‑137 (MQ=255) aCCCGCTGCAAGAAACATAATTAACAAAACCGCGCGCAATGCCCGAATTTCAAGGCTCTCAGCCAGTTACGAACGTTTTTTGTACTTAATCCTACGAATAAATTTGCGACAGAAATTTTACTTGCACCAGGTTTAcc > 4:38678/1‑137 (MQ=255) cGCTGCAAGAAACATAATTAACAAAACCGCGCGCAATGCCCGAATTTCAAGGCTCTCAGCCAGTTACGAACGTTTTTTGTACTTAATCCTACGAATAAATTTGCGACAGAAATTTTACTTGCACCAGGTTTACCCAt < 6:219194/137‑1 (MQ=255) gCAAGAAACATAATTAACAAAACCGCGCGCAATGCCCGAATTTCAAGGCTCTCAGCCAGTTACGAACGTTTTTTGTACTTAATCCTACGAATAAATTTGCGACAGAAATTTTACTTGCACCAGGTTTACCCATaaaa > 1:37767/1‑137 (MQ=255) cAAGAAACATAATTAACAAAACCGCGCGTAATGCCCGAATTTCAAGGCTCTCAGCCAGTTACGAACGTTTTTTGTACTTAATCCTACGAATAAATTTGCGACAGAAATTTTACTTGCACCAGGTTTACCCATAAAAt > 1:159134/1‑137 (MQ=255) cAAGAAACATAATTAACAAAACCGCGCGCAATGCCCGAATTTCAAGGCTCTCAGCCAGTTACGAACGTTTTTTGTACTTAATCCTACGAATAAATTTGCGACAGAAATTTTACTTGCACCAGGt < 3:151419/124‑1 (MQ=255) cAAGAAACATAATTAACAAAACCGCGCGCAATGCCCGAATTTCAAGGCTCTCAGCCAGTTACGAACGTTTTTTGTACTTAATCCTACGAATAAATTTGCGACAGAAATTTTACTTGCACCAGGt > 4:151419/1‑124 (MQ=255) aaGAAACATAATTAACAAAACCGCGCGCAATGCCCGAATTTCAAGGCTCTCAGCCAGTTACGAACGTTTTTTGTACTTAATCCTACGAATAAATTTGCGACAGAAATTTTACTTGCACCAGGTTTACCCATAAAATc > 2:82148/1‑137 (MQ=255) aaGAAACATAATTAACAAAACCGCGCGCAATGCCCGAATTTCAAGGCTCTCAGCCAGTTACGAACGTTTTTTGTACTAAATCCTACGAATAAATTTGCGACAGAAATTTTACTTGCACCAGGTTTACCCATAAAATc > 4:253566/1‑137 (MQ=255) aaGAAACATAAATAACAAAACCGCGCGCAATGCCCGAATTTCAAGGCTCTCAGCCAGTTACGAACGTTTTTTGTACTTAATCCTACGAATAAATTTGCGACAGAAATTTTACTTGCACCAGGTTTACCCATAAAATc > 6:321188/1‑137 (MQ=255) aTTAACAAAACCGCGCGCAATGCCCGAATTTCAAGGCTCTCAGCCAGTTACGAACGTTTTTTGTACTTAATCCTACGAATAAATTTGCGACAGAAATTTTACTTGCACCAGGTTTACCCATAAAATCAGCGCGATTg > 1:86358/1‑137 (MQ=255) aTTAACAAAACCGCGCGCAATGCCCGAATTTCAAGGCTCTCAGCCAGTTACGAACGTTTTTTGTACTTAATCCTACGAATAAATTTGCGACAGAAATTTTACTTGCACCAGGTTTACCCATAAAATCAGCGCGATTg > 3:313393/1‑137 (MQ=255) aaaCCGCGCGCAATGCCCGAATTTCAAGGCTCTCAGCCAGTTACGAACGTTTTTTGTACTTAATCCTACGAATAAATTTGCGACAGAAATTTTACTTGCACCAGGTTTACCCATAAAATCAGCGCGATTGATCCGGc > 7:41272/1‑137 (MQ=255) aaaCCGCGCGCAATGCCCGAATTTCAAGGCTCTCAGCCAGTTACGAACGTTTTTTGTACTTAATCCTACGAATAAATTTGCGACAGAAATTTTACTTGCACCAGGTTTACCCATAAAATCAGCGCGATTGATCCGGc < 6:325464/137‑1 (MQ=255) gcgcgcAATGCCCGAATTTCAAGGCTCTCAGCCAGTTACGAACGTTTTTTGTACTTAATCCTACGAATAAATTTGCGACAGAAATTTTACTTGCACCAGGTTTACCCATAAAATCAGCGCGATTGATCCGGCTGCGa < 4:152299/137‑1 (MQ=255) cgcgCAATGCCCGAATTTCAAGGCTCTCAGCCAGTTACGAACGTTTTTTGTACTTAATCCTACGAATAAATTTGCGACAGAAATTTTACTTGCACCAGGTTTACCCATAAAATCAGCGCGATTGATCCGGCTGCGAc < 7:255260/137‑1 (MQ=255) | GGAATCGCCAGATGAACTTTACAAGGCGCAGAACAGCGAAATTCGAGCGCAGCAGTTCAAATGATATTAATTATCATTACAGGGTTTGCAATTCGTTACCCGCTGCAAGAAACATAATTAACAAAACCGCGCGTAATGCCCGAATTTCAAGGCTCTCAGCCAGTTACGAACGTTTTTTGTACTTAATCCTACGAATAAATTTGCGACAGAAATTTTACTTGCACCAGGTTTACCCATAAAATCAGCGCGATTGATCCGGCTGCGAC > ACB122/4647448‑4647713 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |