Predicted mutation
evidence seq id position mutation annotation gene description
RA ACB122 4,760,718 G→T S33I (AGC→ATC)  vanB → vanillate O‑demethylase oxidoreductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1224,760,7180GT97.3% 246.5 / ‑0.7 73S33I (AGC→ATC) vanBvanillate O‑demethylase oxidoreductase
Reads supporting (aligned to +/- strand):  ref base G (0/2);  new base T (38/33);  total (38/35)
Fisher's exact test for biased strand distribution p-value = 2.26e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.34e-01

GCCACCAGCGCCAACCCTGCCTGAGGAACAGCCGACATGATCGATGCCGTAGTGGTATCCCGTAACGATGAAGCGCAGGGTATCTGCAGCTTCGAGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGACGTGCACCTGCCCGACGGGCTGGTGCGCCAGTATTCGCTGTGCAACCACCCCGAAGAACGCCATCGCTATCTGATTGGCGTACTCAACGACCCGGCTTCGCGGGG  >  ACB122/4760585‑4760841
                                                                                                                                     |                                                                                                                           
gCCACCAGCGCCAACCCTGCCTGAGGAACAGCCGACATGATCGATGCCGTAGTGGTATCCCGTAACGATGAAGCGCAGGGTATCTGCAGCTTCGAGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCAtcgc                                                                                                                          >  8:94264/1‑137 (MQ=255)
gCCACCAGCGCCAACCCTGCCTGAGGAACAGCCGACATGATCGATGCCGTAGTGGTATCCCGTAACGATGAAGCGCAGGGTATCTGCAGCTTCGAGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCAtcgc                                                                                                                          <  8:330588/137‑1 (MQ=255)
gCCACCAGCGCCAACCCTGCCTGAGGAACAGCCGACATGATCGATGCCGTAGTGGTATCCCGTAACGATGAAGCGCAGGGTATCTGCAGCTTCGAGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCAtcgc                                                                                                                          <  3:120129/137‑1 (MQ=255)
     caGCGCCAACCCTGCCTGAGGAACAGCCGACATGATCGATGCCGTAGTGGTATCCCGTAACGATGAAGCGCAGGGTATCTGCAGCTTCGAGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCCTCGCCGgcg                                                                                                                     >  4:6494/1‑137 (MQ=255)
           cAACCCTGCCTGAGGAACAGCCGACATGATCGATGCCGTAGTGGTATCCCGTAACGATGAAGCGCAGGGTATCTGCAGCTTCGAGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCATCGCCGGCGCCCata                                                                                                               >  4:229259/1‑137 (MQ=255)
             aCCCTGCCTGAGGAACAGCCGACATGATCGATGCCGTAGTGGTATCCCGTAACGATGAAGCGCAGGGTATCTGCAGCTTCGAGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATc                                                                                                             <  7:366862/137‑1 (MQ=255)
             aCCCTGCCTGAGGAACAGCCGACATGATCGATGCCGTAGTGGTATCCCGTAACGATGAAGCGCAGGGTATCTGCAGCTTCGAGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATc                                                                                                             <  7:361718/137‑1 (MQ=255)
               cctgcctgAGGAACAGCCGACATGATCGATGCCGTAGTGGTATCCCGTAACGATGAAGCGCAGGGTATCTGCAGCTTCGAGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCATCGCCGGCGCCCATATCGa                                                                                                           >  1:123362/1‑137 (MQ=255)
                 tgcctgAGGAACAGCCGACATGATCGATGCCGTAGTGGTATCCCGTAACGATGAAGCGCAGGGTATCTGCAGCTTCGAGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCATCGCCGGCGCCCATATCGAcc                                                                                                         >  2:355466/1‑136 (MQ=255)
                   cctgAGGAACAGCCGACATGATCGATGCCGTAGTGGTATCCCGTAACGATGAAGCGCAGGGTATCTGCAGCTTCGAGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCATCGCCGGCGCCCATATCGACGTg                                                                                                       <  6:3372/137‑1 (MQ=255)
                      gAGGAACAGCCGACATGATCGATGCCGTAGTGGTATCCCGTAACGATGAAGCGCAGGGTATCTGCAGCTTCGAGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCATCGCCGGCGCCCATATCGACGTGCAc                                                                                                    >  2:2889/1‑137 (MQ=255)
                          aaCAGCCTACATGATCGATGCCGTAGTGGTATCCCGTAACGATGAAGCGCAGGGTATCTGCAGCTTCGAGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCATCGCCGGCGCCCATATCGACGTGCACCTGc                                                                                                <  7:68080/137‑1 (MQ=255)
                                  aCATGATCGATGCCGTAGTGGTATCCCGTAACGATGAAGCGCAGGGTATCTGCAGCTTCGAGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCATCGCCGGCGCCCATATCGACGTGCACCGGCCCGACggg                                                                                        >  6:194740/1‑137 (MQ=255)
                                     tGATCGATGCCGTAGTGGTATCCCGTAACGATGAAGCGCAGGGTATCTGCAGCTTCGAGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCATCGCCGGCGCCCATATCGACGTGCACCTGCCCGACGGGCTg                                                                                     <  1:205864/137‑1 (MQ=255)
                                       ttCGATGCCGTAGTGGTATCCCGTAACGATGAAGCGCAGGGTATCTGCAGCTTCGAGCTGGCCGCGGCAGATGGGAGCCTGCTGCGGGCGTTCATCGCCGGCGCCCATATCGACGTGCACCTGCCCGACGGGCTGGt                                                                                   <  5:56886/136‑1 (MQ=255)
                                       aTCGATGCCGTAGTGGTATCCCGTAACGATGAAGCGCAGGGTATCTGCAGCTTCGAGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCATCGCCGGCGCCCATATCGACGTGCACCTGCCCGACGGGCTGGt                                                                                   <  3:311673/137‑1 (MQ=255)
                                       aTCGATGCCGTAGTGGTATCCCGTAACGATGAAGCGCAGGGTATCTGCAGCTTCGAGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCATCGCCGGCGCCCATATCGACGTGCACCTGCCCGACGGGCTGGt                                                                                   <  5:183347/137‑1 (MQ=255)
                                       aTCGATGCCGTAGTGGTATCCCGTAACGATGAAGCGCAGGGTATCTGCAGCTTCGAGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCATCGCCGGCGCCCATATCGACGTGCACCTGCCCGACGGGCTGGt                                                                                   <  7:372444/137‑1 (MQ=255)
                                       aTCGATGCCGTAGTGGTATCCCGTAACGATGAAGCGCAGGGTATCTGCAGCTTCGAGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCATCGCCGGCGCCCATATCGACGTGCACCTGCCCGACGGGCTGGt                                                                                   <  1:82989/137‑1 (MQ=255)
                                       aTCGATGCCGTAGTGGTATCCCGTAACGATGAAGCGCAGCGTATCTGCAGCTTCGAGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCATCGCCGGCGCCCATATCGACGTGCACCTGCCCGACGGGCTGGt                                                                                   <  1:324189/137‑1 (MQ=255)
                                       aTCGATGCAGTAGTTGTATCCCGTAACGATTAAGCGCAGGGTATCTGCAGCTTCGAGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCATCGCCGGCGCCCATATCGACGTGCACCTGCCCGACGGGCTGGt                                                                                   <  7:254925/137‑1 (MQ=255)
                                         cgctGCCGTAGTGGTAACCCGTAACGATGAAGCGCAGGGTATCTGCAGCTTCGAGCTGGCCGCGGCAGATGTCAGCCTGCTGCCGGCGTTCATCGCCGGCGCCCATATCGACGTGCACCTGCCCGACGGGCTGGTgc                                                                                 <  8:345682/134‑1 (MQ=255)
                                         cGATGCCGTAGTTGTATCCCGTAACGATGAAGCGCAGGGTATCTGCAGCTTCGAGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCATCGCCGGCGCCCATATCGACGTGCACCTGCCCGACGGGCTGGTgc                                                                                 <  2:337286/137‑1 (MQ=255)
                                         cGATGCCGTAGTGGTATCCCGTAACGATGAAGCGCAGGGTATCTGCAGCTTCGAGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCATCGCCGGCGCCCATATCGACGTGCACCTGCCCGACGGGCTGGTgc                                                                                 <  4:259493/137‑1 (MQ=255)
                                          gATGCCGTAGTGGTATCCCGTAACGATGAAGCGCAGGGTATCTGCAGCTTCGAGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCATCGCCGGCGCCCATATCGACGTGCACCAGCCCGACGGGCTGGTgcg                                                                                >  1:91557/1‑137 (MQ=255)
                                            tGCCGTAGTGGTATCCCGTAACGATGAAGCGCAGGGTATCTGCAGCTTCGAGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCATCGCCGGCGCCCATATCGACGTGCACCTGCCCGACGGGCTGGTGCGcc                                                                              >  8:108000/1‑137 (MQ=255)
                                                 tAGTGGTATCCCGTAACGATGAAGCGCAGGGTATCTGCAGCTTCGAGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCATCGCCGGCGCCCATATCGACGTGCACCTGCCCGACGGGCTGGTGCGCCAGTAt                                                                         <  6:118015/137‑1 (MQ=255)
                                                    tGGTATCCCGTAACGATGAAGCGCAGGGTATCTGCAGCTTCGAGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCATCGCCGGCGCCCa                                                                                                                 >  6:107310/1‑94 (MQ=255)
                                                    tGGTATCCCGTAACGATGAAGCGCAGGGTATCTGCAGCTTCGAGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCATCGCCGGCGCCCa                                                                                                                 <  5:107310/94‑1 (MQ=255)
                                                         tCCCGTAACGATGAAGCGCAGGGTATCTGCAGCTTCGAGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCATCGCCGGCGCCCATATCGACGTGCACCTGCCCGACGGGCTGGTGCGCCAGTATTCGCtgtg                                                                 <  7:94264/137‑1 (MQ=255)
                                                         tCCCGTAACGATGAAGCGCAGGGTATCTGCAGCTTCGAGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCATCGCCGGCGCCCATATCGACGTGCACCTGCCCGACGGGCTGGTGCGCCAGTATTCGCtgtg                                                                 <  8:82606/137‑1 (MQ=255)
                                                         tCCCGTAACGATGAAGCGCAGGGTATCTGCAGCTTCGAGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCATCGCCGGCGCCCATATCGACGTGCACCTGCCCGAACGGCTGGTTCGCCATTATTAGCtttt                                                                 >  4:6104/1‑134 (MQ=255)
                                                          cccGTAACGATGAAGCGCAGGGTATCTGCAGCTTCGAGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCATCGCCGGCGCCCATATCGACGTGCACCTGCCCGACGGGCTGGTGCGCCAGTATTCGCTGTGc                                                                <  5:266902/137‑1 (MQ=255)
                                                          cccGTAACGATGAAGCGCAGGGTATCTGCAGCTTCGAGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCATCGCCGGCGCCCATATCGACGTGCACCTGCCCGACGGGCTGGTGCGCCAGTATTCGCTGTGc                                                                <  3:67227/137‑1 (MQ=255)
                                                          cccGTAACGATGAAGCGCAGGGTATCTGCAGCTTCGAGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCATCGCCGGCGCCCATATCGACGTGCACCTGCCCGACGGGCTGGTGCGCCAGTATTCGCTGTGc                                                                <  8:171371/137‑1 (MQ=255)
                                                           ccGTAACGATGAAGCGCAGGGTATCTGCAGCTTCGAGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCATCGCCGGCGCCCATATCGACGTGCACCTGCCCGACCGGCTTGTGCGCCAGTATTCGCTGTTCa                                                               >  6:112785/1‑137 (MQ=255)
                                                            cGTAACGATGAAGCGCAGGGTATCTGCAGCTTCGAGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCATCGCCGGCGCCCATATCGACGTGGCCCAGCCCGCCGGGCTGGTGCGGCAGTATTCGGTGTGCac                                                              >  1:300362/1‑136 (MQ=255)
                                                            cGTAACGATGAAGCGCAGGGTATCTGCAGCTTCGAGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCATCGCCGGCGCCCATATCGACGTGCCCCTGCCCGACGGGCTGGTGCGCCAGTATTCGCTGTGCaa                                                              >  4:256226/1‑137 (MQ=255)
                                                            cGTAACGATGAAGCGCAGGGTATCTGCAGCTTCGAGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCATCGCCGGCGCCCATATCGACGTGCACCTGCCCGACGGGCTGGTGGGCCAGTATTCGCTGTGCaa                                                              >  3:317849/1‑137 (MQ=255)
                                                            cGTAACGATGAAGCGCAGGGTATCTGCAGCTTCGAGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCATCGCCGGCGCCCATATCGACGTGCACCTGCCCGACGGGCTGGTGCGCCAGTATTCGCTGTGCaa                                                              >  8:191/1‑137 (MQ=255)
                                                            cGTAACGATGAAGCGCAGGGTATCTGCAGCTTCGAGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCATCGCCGGCGCCCATATCGACGTGCACCTGCCCGACGGGCTGGTGCGCCAGTATTCGCTGTGCaa                                                              >  1:211957/1‑137 (MQ=255)
                                                              tAACGATGAAGCGCAGGGTATCTGCAGCTTCGAGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCATCGCCGGCGCCCATATCGACGTGCACCTGCCCGACGGGCTGGTGCGCCAGTATTCGCTGTGCAacc                                                            <  1:347596/137‑1 (MQ=255)
                                                              tAACGATGAAGCGCAGGGTATCTGCAGCTTCGAGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCATCGCCGGCGCCCATATCGACGTGCACCTGCCCGACGGGCTGGTGCGCCAGTATTCGCTGTGCAacc                                                            >  3:235955/1‑137 (MQ=255)
                                                                   aTGAAGCGCAGGGTATCTGCAGCTTCGAGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCATCGCCGGCGCCCATATCGACGTGCACCTGTCCGACGGGCTGGTGCGCCAGTATTCGCTGTGCAACCAcccc                                                       >  8:370356/1‑137 (MQ=255)
                                                                        gcgcAGGGTATCTGCAGCTTCGAGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCATCGCCGGCGCCCATATCGACGTGCACCTGCCCGACGGGCTGGTGCGCCAGTATTCGCTGTGCAACCACCCCgaaga                                                  <  2:255905/137‑1 (MQ=255)
                                                                        gcgcAGGGTATCTGCAGCTTCGAGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCATCGCCGGCGCCCATATCGACGTGCACCTGCCCGACCGGCTGGTGCGCCAGTATTCGCTGTGCCACCACCCCgaaga                                                  >  4:153580/1‑137 (MQ=255)
                                                                            aGGGTATCTGCAGCTTCGAGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCATCGCCGGCGCCCATATCGACGTGCCCCTGCCCGACCGGCTGGTGCGGCAGTATTCGCTGTGCCACCACCCCGCAGAACGc                                              >  5:342204/1‑137 (MQ=255)
                                                                              ggTATCTGCAGCTTCGAGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCATCGCCGGCGCCCATATCGACGTGCACCTGCCCGACGGGCTGGTGCGCCAGTATTCGCTGTGCAACCACCCCGAAGAACGCCa                                            <  6:70782/137‑1 (MQ=255)
                                                                                     gCAGCTTCGAGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCATCGCCGGCGCCCATATCGACGTGCACCTGCCCGACGGGCTGGTGCGCCAGTATTCGCTGTGCAACCACCCCGAAGAACGCCATCGCTAt                                     >  3:234149/1‑137 (MQ=255)
                                                                                      cAGCTTCGAGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCATCGCCGGCGCCCATATCGACGTGCACCTGCCCGACGGGCTGGTGCGCCAGTATTCGCTGTGCAACCACCCCGAAGAACGCCATCGCTATc                                    >  4:104274/1‑137 (MQ=255)
                                                                                         cTTCGAGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCATCGCCGGCGCCCATATCGACGTGCACCTGCCCGACGGGCTGGTGCGCCAGTATTCGCTGTGCAacc                                                            <  8:247547/110‑1 (MQ=255)
                                                                                         cTTCGAGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCATCGCCGGCGCCCATATCGACGTGCACCTGCCCGACGGGCTGGTGCGCCAGTATTCGCTGTGCAacc                                                            >  7:247547/1‑110 (MQ=255)
                                                                                         cTTCGAGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCATCGCCGGCGCCCATATCGACGTGCACCTGCCCGACGGGCTGGTGCGCCAGTATTCGCTGTGCAACCACCCCGAAGAACGCCATCGCTATCTGa                                 >  6:343859/1‑137 (MQ=255)
                                                                                           tCGAGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCATCGCCGGCGCCCATATCGACGTGCACCTGCCCGCCGGGCTGGTGGGCCAGTATTCGCTGTGCAACCCCCCCGAAGAACGCCCTCGCTATCTGAtt                               >  2:18620/1‑137 (MQ=255)
                                                                                           tCGAGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCATCGCCGGCGCCCATATCGACGTGCACCTGCCCGACGGGCTGGTGCGCCAGTTTTCGCTGTGCAACCCCCCCGAAGAACGCCCTCGCTATCTGAtt                               >  3:220566/1‑137 (MQ=255)
                                                                                           tCGAGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCATCGCCGGCGCCCATATCGACGTGCACCTGCCCGACGGGCTGGTGCGCCAGTATTCGCTGTGCAACCACCCCGAAGAACGCCATCGCTATCTGAtt                               >  5:130800/1‑137 (MQ=255)
                                                                                           tCGAGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCATCGCCGGCGCCCATATCGACGTGCACCTGCCCGACGGGCTGGTGCGCCAGTATTCGCTGTGCAACCACCCCGAAGAACGCCATCGCGATCTGAtt                               >  2:203481/1‑137 (MQ=255)
                                                                                           tCGAGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCATCGCCGGCGCCCATATCGACGTGCACCTGCCCGACGGGCTGGTGCGCCAGTATTAGCTGTGCCACCACCCCGAAGAACGCGATCGCGAACAGAtt                               >  4:239502/1‑137 (MQ=255)
                                                                                           tCGAGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCATCGCCGGCGCCCATATCGACGTGCACCTGCCCGACCGGCTGGTGGGCCAGTATTCGCTGTGCAACCACCCCCAAGAACGCCAACGCTATCTTAtt                               >  7:177860/1‑137 (MQ=255)
                                                                                            cGAGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCATCGCCGGCGCCCATATCGACGTGCACCTGCCCGACGGGCTGGTGCGCCGGTATTCGCTGTGCAACCACCCCGAAGAACGCCATCGCTATCTGATTg                              <  2:145076/137‑1 (MQ=255)
                                                                                             gAGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCATCGCCGGCGCCCATATCGACGTGCACCTGCCCGACGGGCTGGTGCGCCAGTATTCGCTGTGCAACCACCCCGAAGAACGCCATCGCTATCTGATTgg                             >  6:128857/1‑137 (MQ=255)
                                                                                                 tGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCATCGCCGGCGCCCATATCGACGTGCCCCTGCCCGCCGGGCTGGTGCGCCAGTATTCGCTGTGCAACCACCCCGAAGAACGCCATCGCTATCTGATTGGCGTa                         >  6:263584/1‑137 (MQ=255)
                                                                                                 tGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCATCGCCGGCGCCCATATCGACGTGCACCTGCCCGCCGGGCGGGTGGGCCCGTATTGGCTGTGCAACCCCCCCCAAGAACGCCCTCGCTATCTGATTGGCGtt                         >  4:218361/1‑136 (MQ=255)
                                                                                                 tGGCCGCGACAGATGGCAGCCTGCTGCCGGCGTTCATCGCCGGCGCCCATATCGACGTGCACCTGCCCGACGGGCTGGTGCGCCCGTATTCGCTGTGCAACCCCCCCGAAGAACGCCATCGCTATCTGATTGGCGTa                         >  6:157094/1‑137 (MQ=255)
                                                                                                      gcgGCAGATGGCAGCCTGCTGCCGGCGTTCATCGCCGGCGCCCATATCGACGTGCCCCGGCCCGCCGGGCTGGTGCGCCAGTTTTCGCTGTGGAACCCCCCCGAAGAACGCCATCGCTATCTGATTGGCGGACGCaa                    >  4:36541/1‑137 (MQ=255)
                                                                                                          cAGATGGCAGCCTGCTGCCGGCGTTCATCGCCGGCGCCCATATCGACGTGCACCTGCCCGACGGGCTGGTGCGCCAGTATTCGCTGTGCAACCACCCCGAAGAACGCCATCGCTATCTGATTGGCGTACTCAACGAc                <  1:338083/137‑1 (MQ=255)
                                                                                                           aGATGGCAGCCTGCTGCCGGCGTTCATCGCCGGCGCCCATATCGACGTGCACCTGCCCGACGGGCTGGTGCGCCAGTATTCGCTGTGCAACCACCCCGAAGAACGCCATCGCTATCTGATTGGCGTACTCAACGAcc               <  3:251809/137‑1 (MQ=255)
                                                                                                           aGATGGCAGCCTGCTGCCGGCGTTCATCGCCGGCGCCCATATCGACGTGCACCTGCCCGACGGGCTGGTGCGCCAGTATTCGCTGTGCAACCACCCCGAAGAACGCCATCGCTATCTGATTGGCGTACTCAACGAcc               >  2:244436/1‑137 (MQ=255)
                                                                                                             tgggCAGCCTGCTGCCGGCGTTCATCGCCGGCGCCCATATCGACGTGCACCTGCCCGACGGGCTGGTGCGCCAGTATTCGCTGTGCAACCACCCCGAAGAACGCCATCGCTATCTGATTGGCGTACTCAACGACCCg             <  2:170420/135‑1 (MQ=255)
                                                                                                                     ctgctgCCGGCGTTCATCGCCGGCGCCCATATCGACGTGCACCTGCCCCACGGGCTGGTGCGCCAGTATTCGCTGTGCAACCACCCCGCAGAACGCCATCGCGATCTGATTGGCGTACTCAACCACCCGGCTTcgcg     >  5:126922/1‑137 (MQ=255)
                                                                                                                      tgctgcCGGCGTTCATCGCCGGCGCCCATATCGACGTGCACCTGCCCGACGGGCTGGTGCGCCAGAATTCGCTGTGCAACCACCCCGAAGAACGCCATCGCTATCTGATTGGCGTACTCAACGACCCGGCTTCGCgg    <  6:148250/137‑1 (MQ=255)
                                                                                                                       gctgcCGGCGTTCATCGCCGGCGCCCATATCGACGTGCACCAGCCCGACGGGGTGGTGCGCCAGTATTCGCTGTGCAACCACCCCGAAGAACGCCATCGCTATCTTATTGGCGTACTCAACGACCCGGCTTAGCggg   >  6:354540/1‑137 (MQ=255)
                                                                                                                        gtgcCGGCGTTCATCGCCGGCGCCCATATCGACGTGCACCTGCCCGACGGGCTGGTGCGCCAGTATTCGCTGTGCAACCACCCCGAAGAACGCCATCGCTATCTGATTGGCGTACTCAACGACCCGGCTTCGCgggg  <  2:20972/136‑1 (MQ=255)
                                                                                                                        gtgcCGGCGTTCATCGCCGGCGCCCATATCGACGTGCACCTGCCCGACGGGCTGGTGCGCCAGTATTCGCTGTGCAACCACCCCGAACAACGCCATCGCTATCTGATTGGCGTACTCAACGACCCGGCTTCGCgggg  <  8:55156/136‑1 (MQ=255)
                                                                                                                        ctgcCGGCGTTCATCGCCGGCGCCCATATCGACGTGCACCTGCCCGACGGGCTGGTGCGCCAGTATTCGCTGTGCAACCACCCCGAAGAACGCCATCGCTATCTGATTGGCGTACTCAACGACCCGGCTTCGCgggg  <  3:104274/137‑1 (MQ=255)
                                                                                                                        ctgcCGGCGTTCATCGCCGGCGCCCATATCGACGTGCACCTGCCCGACGGGCTGGTGCGCCAGTATTCGCTGTGCAACCACCCCGAAGAACGCCATCGCTATCTGATTGGCGTACTCAACGACCCGGCTTCGCgggg  <  1:355466/137‑1 (MQ=255)
                                                                                                                                     |                                                                                                                           
GCCACCAGCGCCAACCCTGCCTGAGGAACAGCCGACATGATCGATGCCGTAGTGGTATCCCGTAACGATGAAGCGCAGGGTATCTGCAGCTTCGAGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGACGTGCACCTGCCCGACGGGCTGGTGCGCCAGTATTCGCTGTGCAACCACCCCGAAGAACGCCATCGCTATCTGATTGGCGTACTCAACGACCCGGCTTCGCGGGG  >  ACB122/4760585‑4760841

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: