Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	ACB143	5,760,598	(A)6→5	coding (781/834 nt)	CJ019_05400 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	ACB143	5,760,593	0	A	.	89.1%	168.2 / 15.6	47	coding (776/834 nt)	CJ019_05400	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base . (26/15);  minor base T (0/5);  total (26/21)
Fisher's exact test for biased strand distribution p-value = 1.13e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AAGCGCGCGAACAGGACCAGGCCGACCTGGCCAAGGTCTTGAAGACCATCGAGGAAACCCTCGCCCGCCAGGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGGAAAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAACCGCCGAAAAAGGCCCGTACCACCCTCGGGCCGATGGTTTCCAGCGATGGC  >  ACB143/5760467‑5760721                                                                                                                               |                                                                                                                                 aaGCGCGCGAACAGGACCAGGCCGACCTGGCCAAGGTCTTGAAGACCATCGAGGAAACCCTCGCCCGCCAGGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGGaaaaacgc                                                                                                                           <  1:261681/134‑4 (MQ=255)  aGCGCGCGAACAGGACCAGGCCGACCTGGCCAAGGTCTTGAAGACCATCGAGGAAACCCTCGCCCGCCAGCCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGG‑AAAAAcgccg                                                                                                                        >  2:257919/1‑135 (MQ=255)                   tggCCGACCTGGCAAAGGTCTTGAAGACAATCTAGGAACCCCTCGCCCGCACGGCCGGTGACGCTGAAGAGCCACGCAAGAAGGCCCTGCTGGCGCAGCAGTAAGC‑GTAAAAACGCCGCCAGCAGGAAGCGCTgg                                                                                                       <  2:247590/134‑1 (MQ=255)                    ggCCGACCTGGACAAGGTCTTTAAGACCATCGAGGAAACCCTCGCCCGCCAGGCCCGTGAAGCTGATGAGGCACGCAAGAAGGCCCTGCTGGCTCAGAATGAATC‑TTAAAAACGCCGCCAGCAGGAAGCGCTGgcg                                                                                                     <  4:362479/136‑2 (MQ=255)                     gCCGACCTGGCCAAGGTCTTGAAGACCATCGAGGAAACCCTCGCCCGCCAGGCCCGTGAAGCTGAAGAGGCACGAAAGAAGGCCCTGCTGGCGAAGAAGTAAGCTG‑AAAAACGCCGCCAGCAGGAAGCGCTGgcg                                                                                                     <  8:44534/135‑2 (MQ=255)                        gACCTGGCCAAGGTCTTGAAGACCATCGAGGAAACCCTCGCCCGCCAGGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGG‑AAACACGCCGCCAGCAGGAAGCGCTGgccgcc                                                                                                  >  5:249359/1‑135 (MQ=255)                          ccTGGCCAAGGTCTTGAAGACCATCGAGGAAACCCTCGCCCGCCAGGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGgccgccgc                                                                                                >  8:228814/1‑135 (MQ=255)                            tGGCCAAGGTCTTGAAGACCATCGAGGAAACCCTCGCCCGCCAGGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCGGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCcgccgccgc                                                                                             >  3:229612/1‑136 (MQ=255)                                   ggTCTTGACGACCATCGAGCAACCCCTCGCCCGCCAGGCCCGTGACGCGCAAGCGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCcg                                                                                       <  5:320302/135‑1 (MQ=255)                                      cTTGAAGACCATCGAGGAAACCCTCGCCCGCAAGGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCCCCGCCGCTCCCcgcgc                                                                                    >  2:323996/1‑134 (MQ=255)                                           aGACCATCGAGGAAACCCTCGCCCGCCAGGCCCGTGAAGCTGAAGAGGCCCGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCggg                                                                               >  8:181451/1‑135 (MQ=255)                                           aGACCATCGAGGAAACCCTCGCCCGCCAGGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGc                                                                              >  3:383096/1‑136 (MQ=255)                                            gACCATCGAGGAAACCAGCGCCCGCCAGGCCCGTGAAGCTGAACAGGCACGCAAGAAGGCCCTGGTGGCGCAGCAGGATGCGG‑AAAAACCCCGCCAGCAGGAAGCGCTGGCCGCCGCGGCTGCCCGCGACCGGGc                                                                              <  8:90724/135‑1 (MQ=255)                                               cATCGAGGAAACCCGCGCCCGCCAGGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCg                                                                           <  1:73307/135‑1 (MQ=255)                                                  cGAGGAAACCCTCGCCCGCCAGGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGTAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGa                                                                        >  1:96762/1‑135 (MQ=255)                                                  cGAGGAAACCCTCGCCCGCCAGGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGa                                                                        >  2:180343/1‑135 (MQ=255)                                                  cGAGGAAACCCTCGCCCGCCAGGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGa                                                                        >  5:129754/1‑135 (MQ=255)                                                  cGAGGAAACCCTCGCCCGCCAGGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGa                                                                        >  2:391527/1‑135 (MQ=255)                                                  cGAGGAAACCCTCGCCCGCCAGGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAg                                                                       >  4:374160/1‑136 (MQ=255)                                                    agcaaccccGCGCCCGCCCGGCCGGTGAAGCGCACGAGGCCCCCAAGACGGCCCTGCGGGCGCAGCAGTACGC‑GTAAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGc                                                                      <  1:180320/129‑1 (MQ=255)                                                    aGGAAACCCTCGCCCGCCAGGCCCGTGAAGCTGAAGAGGCTCGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCCGGCCCGTGAGc                                                                      >  7:235896/1‑135 (MQ=255)                                                    aGGAAACCCTCGCCCGCCAGGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTgcg                                                                       >  7:230752/1‑132 (MQ=255)                                                    aGGAAACCCTCGCCCGCCAGGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGc                                                                      >  2:389282/1‑135 (MQ=255)                                                       acaCCCCCGCCCGCCAGCCCCGTCATGCTCAACCGGCACGCACGAAGGCCCTGCGGGCGCAGCGGGAAGCGG‑AAAACCCCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGc                                                                  <  4:344567/134‑1 (MQ=255)                                                       aaaCCCTCGCCCGCCAGGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGc                                                                  <  4:383229/136‑1 (MQ=255)                                                        aaCCCTCGCCCGCCAGGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGc                                                                  <  4:284349/135‑1 (MQ=255)                                                                 ccgccAGGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGG‑AAAAACGCAGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGt                                                         >  2:375960/1‑135 (MQ=255)                                                                           cGTGAAGCTGAAGAGGCACGCAACAAGGCCCTGCTGGCGCAGCAGGAAGC‑GTAAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAAccgccg                                               <  8:37459/135‑1 (MQ=255)                                                                           cGTGAAGATTAAGAGGCACGCAAGAAGGCCCTGATGGCGCAGCTGTAAG‑GTTAAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAAccgccg                                               <  7:219811/135‑1 (MQ=255)                                                                               aaGCTGACGAGGCACGCAAGAAGGCCCTGCGGGCGCAGCAGGAAG‑GGGAAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAACCGCCGaaaaa                                          <  3:76234/136‑1 (MQ=255)                                                                                aGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAACCGCCGaaaaa                                          <  7:202291/135‑1 (MQ=255)                                                                                     aaGAGGCACGCAAGACGGCCCTGCTGGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAACCGCCGAAAAAGGCCCg                                    <  3:308137/136‑1 (MQ=255)                                                                                     aaGAGCCCCGCACGAAGGCCCGGCTGGCGCAGCAGGACGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAACCGCCGAAAAAGGCCCg                                    <  3:265097/136‑1 (MQ=255)                                                                                       gagGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAACCGCCGAAAAAGGCCCGt                                   >  1:400515/1‑135 (MQ=255)                                                                                            aCGAAAGATGGCCCTGCTGGCGCAGCAGTAAGCGG‑AAAACCGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAACCGCCGAAAAAGGCCCGTaccac                              <  5:145735/135‑1 (MQ=255)                                                                                               cAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCt                                                                    <  1:33036/94‑1 (MQ=255)                                                                                               cAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCt                                                                    >  2:33036/1‑94 (MQ=255)                                                                                                aagaagGCCCTGCTGGTGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAACCGCCGAAACAGGCCCGTACCACCCTc                          >  1:166273/1‑135 (MQ=255)                                                                                                  gaagGCCCTGCTGGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGTCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAACCGCCGAAAAAGGCCCGTACCACCCTCggg                       >  4:314700/1‑136 (MQ=255)                                                                                                  gaagGCCCTGCTGGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAACCGCCGAAAAAGGCCCGTACCACCCTCgg                        >  5:294355/1‑135 (MQ=255)                                                                                                             tgGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGCGCCTGCAGAGCCAGTGGCACCGCCGAAAAAGGCCCGTACCACCCTCGGGCCGATGGttt             >  1:267729/1‑135 (MQ=255)                                                                                                             tgGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAACCGCCGAAAAAGGCCCGTACCACCCTCGGGCCGATGGttt             >  7:38430/1‑135 (MQ=255)                                                                                                             tgGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAACCGCCGAAAAAGGCCCGTACCACCCTCGGGCCGATGGTTTc            >  3:239594/1‑136 (MQ=255)                                                                                                               gcgcAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGCACCGCCGAAAAAGGCCCGTACCACCCTCGGGCCGATGGTTTcc           >  6:238662/1‑135 (MQ=255)                                                                                                                   agcagGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAACCGCCGAAAAAGGCCCGTACCACCCTCGGGCCGATGGTTTCCAGCg       >  2:1780/1‑135 (MQ=255)                                                                                                                     cagGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAACCGCCGAAAAAGGCCCGTACCACCCTCGGGCCGATGGTTTCCAGCGATg    <  4:23520/136‑1 (MQ=255)                                                                                                                       ggACGCGG‑AAAAACGCCGCCTGCAGGATGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGGGAGCCTGCAGAGCCAGTGGAACCGCCGAAAAAGGCCCGTACCACCCTCGGGCCGATGGTTTCCAGCGATGgc  <  3:279700/136‑1 (MQ=255)                                                                                                                         aaGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAACCGCCGAAAAAGGCCCGTACCACCCTCGGGCCGATGGTTTCCAGCGATGgc  <  1:391527/134‑1 (MQ=255)                                                                                                                               |                                                                                                                                 AAGCGCGCGAACAGGACCAGGCCGACCTGGCCAAGGTCTTGAAGACCATCGAGGAAACCCTCGCCCGCCAGGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGGAAAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAACCGCCGAAAAAGGCCCGTACCACCCTCGGGCCGATGGTTTCCAGCGATGGC  >  ACB143/5760467‑5760721

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑