| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | ACB143 | 3,626,089 | 0 | T | G | 56.0% | ‑1.5 / 13.2 | 25 | V174G (GTG→GGG) | hcf136_3 | Ycf48‑like protein |
| Reads supporting (aligned to +/- strand): ref base T (5/6); new base G (2/12); total (7/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.77e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.96e-01 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CGAGGCTGCCGCAGACGAGCGTCGCCTGGCGGCGGCGCAACGGCTGCTGGCCGACGGCGCGGACAAGCCGTTGTTGGCCCTGAATTT‑CACCGATGCCCGCAACGGCCTGGTCGTCGGTGCCTACGGGGTGGCCA‑TGCGCACCGACGACGGCGGGCGCAGCTGGCAGTCGGAGATCGGCCGACTGCCGAACGGGCGTGGACTGCACCTGTATGCCCTGGCGCGCCAAGGCAACCAGGTGTACATCGCCGGCGAGCAGGG > ACB143/3625961‑3626218 | cGAGGCTGCCGCAGACGAGCGTCGCCTGGCGGCGGCGCAACGGCTGCTGGCCGACGGCGCGGACAAGCCGTTGTTGGCCCTGAATTT‑CACCGATGCCCGCAACGGCCTGGTCGTCGGTGCCGACGGGGGGGCCA‑Tg > 3:219182/1‑136 (MQ=255) aGGCTGCCGCAGACGAGCGTCGCCTGGCGGCGGCGCAACGGCTGCTGGCCGCCGGCGCGGACAAGCCGTTGTTGGCCCGGAATTT‑CACCGATGCCCGCCACGGCCTGGGCCTCGGTGCCGAGGGGGGGGCCA‑Tgc > 6:315785/1‑135 (MQ=255) cGCCTGGCGGCGGCGCAACGGCTGCTGGCCGACGGCGCGGACAAGCCGTTGTTGGCCCTGAATTT‑CACCGATGCCCGCAACGGCCTGGTCGTGGGTGCCGACGGGGTGGCCA‑TGCGCCCCGACGCCGGCGGgcgc > 5:107502/1‑135 (MQ=255) ccgccgGCGGGTACACGCGGTTGTTGGCCCTTAATTC‑CACCGAGCCCCCCCCCGGCGGGGGCGGCGGGGCCTAGGGGGGGCCCA‑TGCGCACCGACGACGGCGGGCGCAGCTGGCGGTCGGAGATCGGCCGACTGcc < 3:278045/136‑1 (MQ=255) ggACGGCGCGGAGAAGCCGTTGTTGGCCCTTAATTT‑CAACGATGCCCGCTAGGGCCTGGTCGTCGGTGCCTAGGGGGTGGCCA‑TGCGCGCCGACGACGGCTGGCGCAGCTGGCAGTCGGAGATCGGCCGACTGcc < 2:379011/134‑1 (MQ=255) ccagggCGCGCACACCCCGTGGTGGGCCCTGAATTC‑CCCCCCGGCCCCAACCGGCGTGGGCGCGGGGGCCTCCGGGGGGGCAA‑GGCCCCCCGACGACGGCGGGCGCAGCTGGCAGTCGGAGATCGGCCGACTGcc < 6:274688/131‑1 (MQ=255) cGACGGCGCGGACAAGCCGTTGTTGGCCGTGATTAT‑CACCGATGCCCGCAACGGCCTGGGCGGCGGTGCCTACGGGGGGGCCA‑TGCGCACCGACGACGGCGGGCGCAGCTGGCAGTCGGAGATCGGCCGACTGcc < 2:323635/135‑1 (MQ=255) gggCGGGGACACGCGGTGGTGGGCCATCAATTA‑CCCCGAGCCCCCCACCGGCGGGGGCGGGGGGCCCTAGGGGGGGGCCA‑GGCGCACCGACGACGGCGGGCGCAGCTGGCAGTCGGAGATCGGCCGACTGCCGaa < 8:76517/134‑1 (MQ=255) aaGCGGTTGTTGGCCCTTAATTT‑CACCGATGCCCGCACCGGCGGGGTCGTCGGTGCCTACGGGGTGGCAA‑TGCGCACCGACGACGGCGGGCGCAGCTGGCAGTCGGAGATCGGCCGACTGCCGAACGGGCGTGGa < 5:149521/135‑1 (MQ=255) gcggttgtgGGCCCTGAATTG‑CCCCGAGCCCCGCACCGGCGGGGGCGTCGGTGCCTCGGGGGGGGCCA‑GGCGCACCGACGACGGCGGGCGCAGCTGGCAGTCGGAGATCGGCCGACTGCCGAACGGGCGTGGACt < 2:17528/132‑1 (MQ=255) gCCGTTGTGGGCCCTGAATTT‑CACCGATGCCCGCACCGGCCGGGGCGGCGGGGCCTAGGGGGGGGCCA‑TGCGCACCGACGACGGCGGGCGCAGCTGGCAGTCGGAGATCGGCCGACTGCCGAACGGGCGTGGACTg < 4:306539/136‑1 (MQ=255) gcGTTTTTTGCCCTTTATTTACCCCTATGCCCCCACCGGGTTGGGGGTGGGGGCCTCGGGGGGGGCCA‑TCCGCACCGAGGACGGCGGGCGCAGCTGGCAGTCGGAGATCGGCCGACTGCCGAACGGGCGTGGACt < 1:163766/134‑1 (MQ=255) tgtggGCCCTGAATTT‑CCCCGATGCCCGCACCGGCGTGGGCGGCGGTGCCTACGGGGTGGCCA‑TGCGCACCGACGACGGCGGGCGCAGCTGGCAGTCGGAGATCGGCCGACTGCCGAACGGGCGTGGACTGCAcc < 6:97159/135‑1 (MQ=255) ggtgggCCCTCATTTT‑CCCCGAGGCCCGCACCGGCCGGGGGGTGGGTGCCTACGGGGGGGCCT‑GGCCCACCGACGACGGCGGGCGCAGCTGGCAGTCGGAGATCGGCCGACTGCCGAACGGGCGTGGACTGCAcc < 6:138499/131‑1 (MQ=255) ggCCCTGAATTT‑CACCGATGCCCGCAACGGCCTGGTCGTCGGTGCCTACGGGGTGGCCA‑AGCGCACCGACGACGGCCGGCGCAGCTGGCAGTCGGAGATCGGCCGACTGCCGAACGGGCGTGGACTGCACCTGTAt > 3:218094/1‑136 (MQ=255) cGAGGCCCGCACCGGCGGGGTGGGGGGGGCCTCGGGGGTGGCCT‑GGCGCCCCGACGACGGCGGGCGCAGCTGGCAGTCGGAGATCGGCCGACTGCCGAACGGGCGTGGACTGCACCTGTATGCCCTGGCGCGCCa < 7:181103/135‑1 (MQ=255) tGCCCGCAACGGCCGGGGCGGCGGTGCCTACGGGGTGGCCA‑GGCGCACCGACGACGGCGGGCGCAGCTGGCAGTCGGAGATCGGCCGACTGCCGAACGGGCGTGGACTGCACCTGTATGCCCTGGCGCGCCAAGGc < 3:83485/136‑1 (MQ=255) gCCCCCAACGCCCGGGGCGGCGGGGCCTCGGGGGTGGCCA‑TCCGCACCGACGACGGCGGGCGCAGCTGGCAGTCGGAGATCGGCCGACTGCCGAACGGGCGTGGACTGCACCTGTATGCCCTGGCGCGCCAAGGc < 8:292667/135‑1 (MQ=255) ccccGCACCGCCCGGGTCTGGGTTGCCT‑CGGGGGGGCCATTCCGCACCTACGACGGCGGGCGCAGCTGGCAGTCGGAGATCGGCCGACTGCCGAACGGGCGTGGACTGCACCTGTATGCCCTGGCGCGCCAAGGc < 5:141617/134‑1 (MQ=255) cccGCACCGGCCTGGCGGGCGGTGCCTACGGGGGGGCCA‑GGCGCACCTACGACGGCGGGCGCAGCTGGCAGTCGGAGATCGGCCGACTGCCGAACGGGCGTGGACTGCACCTGTATGCCCTGGCGCGCCAAGGCa < 6:394854/135‑1 (MQ=255) cccGCAACGGCCTGGTCGTCGGTGCCTACGGGGTGGCCA‑TGCGCACCGACGACGGCGGGCGCAGCTGGCAGTCGGAGATCGGCCGACTGCCGAACGGGCGTGGACTGCACCTGTATGCCCTGGCGCGCCAAGGCa > 5:180123/1‑135 (MQ=255) cccGCAACGGCCTGGTCGTCGGTGCCTACGGGGTGGCCA‑TGCGCACCGACGACGGCGGGCGCAGCTGGCAGTCGGAGATCGGCCGACTGCCGAACGGGCGTGGACTGCACCTGTATGCCCTGGCGCGCCAAGGCa > 1:318423/1‑135 (MQ=255) accGGCCGGGGCGGCGGTGCCTCCGGGGGGCCCT‑GGCCCACCGCCGACGGCGGGCGCAGCTGGCAGTCGGAGATCGGCCGACTGCCGAACGGGCGTGGACTGCACCTGTATGCCCTGGCGCGCCAAGGCAACCAg < 7:217493/133‑1 (MQ=255) ggcggcgGGGCCTCCGGGGGGGCCA‑GGCGCCCCGACGACGGCGGGCGCAGCTGGCAGTCGGAGATCGGCCGACTGCCGAACGGGCGTGGACTGCACCTGTATGCCCTGGCGCGCCAAGGCAACCAGGTGTACATc < 1:35349/130‑1 (MQ=255) cGGGGTGGCCA‑TGCGCACCGACGACGGCGGGCGCAGCTGGCAGTCGGAGATCGGCCGACTGCCGAACGGGCGTGGACTGCACCTGTATGCCCTGGCGCGCCAAGGCAACCAGGTGTACATCGCCGGCGAGCAggg > 5:287383/1‑135 (MQ=255) | CGAGGCTGCCGCAGACGAGCGTCGCCTGGCGGCGGCGCAACGGCTGCTGGCCGACGGCGCGGACAAGCCGTTGTTGGCCCTGAATTT‑CACCGATGCCCGCAACGGCCTGGTCGTCGGTGCCTACGGGGTGGCCA‑TGCGCACCGACGACGGCGGGCGCAGCTGGCAGTCGGAGATCGGCCGACTGCCGAACGGGCGTGGACTGCACCTGTATGCCCTGGCGCGCCAAGGCAACCAGGTGTACATCGCCGGCGAGCAGGG > ACB143/3625961‑3626218 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |