| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | ACB143 | 5,386,513 | +AA | intergenic (‑522/+1129) | CJ019_05070 ← / ← nptII | hypothetical protein/neomycin phosphotransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | ACB143 | 5,386,512 | 1 | . | A | 81.8% | 26.6 / 2.3 | 11 | intergenic (‑521/+1130) | CJ019_05070/nptII | hypothetical protein/neomycin phosphotransferase |
| Reads supporting (aligned to +/- strand): ref base . (1/1); new base A (6/3); total (7/4) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.51e-01 | |||||||||||
| * | ACB143 | 5,386,512 | 2 | . | A | 81.8% | 21.4 / 2.3 | 11 | intergenic (‑521/+1130) | CJ019_05070/nptII | hypothetical protein/neomycin phosphotransferase |
| Reads supporting (aligned to +/- strand): ref base . (1/1); new base A (6/3); total (7/4) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.24e-01 | |||||||||||
AAAAATTGATCGAATTCTTTCATTTAAGACCCTAATATGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCCGTCGT‑‑‑CA‑GG‑‑ATC‑‑ATCC‑GGGCGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTGCGGGTGCCAGGGCGTGCCCTTGGG > ACB143/5386427‑5386605 || aaaaaTTGATCGAATTCTTTCATTTAAGACCCTAATATGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCAGTGGTG‑‑TACGG‑‑TACAAACCC‑CGACGCTAGCATCGCATGAGAAAGCCCCCGGAAGATCACCTTc > 2:277347/1‑137 (MQ=255) cGAATTCTTTCATTTAAGACCCTAATATGACATCTCGCCCGGCTTCTCGACGACGGCGGTCTCAGAGGTGTACG‑GT‑‑ACA‑‑AACCACGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCttt > 3:111579/1‑137 (MQ=255) ttCTTTCATTTAAGACCCTAATATGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCAGTGGTG‑‑TACGG‑‑TACAAACCC‑CGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTa < 7:60037/137‑1 (MQ=255) caCCCTAATATGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCAGTGGTG‑‑TACGG‑‑TACAAACCC‑CGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTGCGGGt < 6:12099/136‑1 (MQ=255) aaTATGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCAGTGGTG‑‑TACGGTAAAA‑‑ACCC‑CGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTGCGGGTGCCatg < 5:293054/137‑3 (MQ=255) aaTATGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCAGTGGTG‑‑TACGG‑‑TACAAACCC‑CGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTGCGGGTGCCAgg < 3:94555/137‑1 (MQ=255) atGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCAGTGGTG‑‑TACGG‑‑TACAAACCC‑CGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGTGCTTTTTTATTGCGCTGCGTGTGCAAGGGCg > 4:192268/1‑137 (MQ=255) atGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCAGTGGTG‑‑TACGG‑‑TACAAACCC‑CGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTGCGGGTGCCAGGGCg > 2:66454/1‑137 (MQ=255) ctcGGCCGGCTTGTCGACGACGGCGGTCTCAGTGGTG‑‑TACGG‑‑TACAAACCC‑CGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTGCGGGTGCCAGGGCGTGCCCtt > 8:236801/1‑137 (MQ=255) ctcGGCCGGCTTGTCGACGACGGCGGTCTCAGTGGTG‑‑TACGG‑‑TACAAACCC‑AGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTGCGGGTGCCAGGGCGTGCCCtt > 5:357066/1‑137 (MQ=255) ggccggcTTGTCGACGACGGCGGTCTCAGTGGTG‑‑TACGG‑‑TACAAACCC‑CGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTGCGGGTGCCAGGGCGTGCCCTTggg > 1:121920/1‑137 (MQ=255) || AAAAATTGATCGAATTCTTTCATTTAAGACCCTAATATGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCCGTCGT‑‑‑CA‑GG‑‑ATC‑‑ATCC‑GGGCGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTGCGGGTGCCAGGGCGTGCCCTTGGG > ACB143/5386427‑5386605 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |