| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | ACB143 | 1,537,450 | 0 | T | G | 57.1% | 1.3 / 10.3 | 14 | W400G (TGG→GGG) | mdfA | Multidrug transporter MdfA |
| Reads supporting (aligned to +/- strand): ref base T (5/1); new base G (1/7); total (6/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.56e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.55e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
ACGATTGCCTTGCTGGGGTTTGGCGGAGCGCTGCTGGCGATGATTGGCGCCGGTGCCAGCTTGCTGCATTTTGCCC‑T‑GGCGGCGGGGGTGGCGGGGGCGT‑TGGCGCTGTGGCCGCTGTGGTTTGTGGTGGGTGGGCGGCCTGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACACAGGGCCGCGCCGCTTTGTA > ACB143/1537333‑1537564 | aCGATTGCCTTGCTGGGGTTTGGCGGAGCGCTGCTGGCGATGATTGGCGCCGGTGCCAGCTTGCTGCATTTTGCCC‑T‑GGCGGCGGGGGTGGCGGGGGCGT‑TGGCGCTGTGGCCGCTGTGGTTTGTGGTGGGTGggcg > 4:98650/1‑137 (MQ=255)aCGATTGCCTTGCTGGGGTTTGGCGGAGCGCTGCTGGCGATGATTGGCGCCGGTGCCAGCTTGCTGCATTTTGCCC‑T‑GGCGGCGGGGGTGGCGGGGGCGT‑TGGCGCTGTGGCCGCTGTGGTTTGTGGTGGGTGggcg > 8:330308/1‑137 (MQ=255)aCGATTGCCTTGCTGCGGTTTGGCGGAGCGCTGCTGGCGATGATTGGCGCCGCTGCAAGCTTGCTGCATTTAGCCC‑T‑GGCGGCGCGGGAGGCGGGGGACT‑TGGCGCTGTGGCCGCTGTGGTTTGTGGTGGGTGgtcg > 7:298163/1‑137 (MQ=255)aCGATTGCCTTCCTGGTGTATGGCGGAGAGCTGCTGGCGATGATTGGCGCCGGTGCCAGCTTTCTGCATTTTGCCC‑T‑CGCGGCGGGGGTGGCGGGGGCGT‑TGGCGCTGTGGCCGCTGTGGGTTGTGGTGGGAGtgcg > 7:298162/1‑137 (MQ=255) tGGCGCCGGTGCCAGCTTGCTGCATTTTGCCC‑T‑GGCGGCGGGGGTGGCGGGGGCGT‑TGGCGCTGTGGCCGCTGTGGTTTGTGGTGGGTGGGCGGCCTGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGggc > 4:45774/1‑137 (MQ=255) tGGCGCCGGTGCCAGCTTGCTGCATTTTGCCC‑T‑GGCGGCGGGGGTGGCGGGGGCGT‑TGGCGCTGTGGCCGCTGGGGTTTGTGGTGGGTGGGCGGCCTGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGggc > 1:61958/1‑137 (MQ=255) gtggcttttGCCCGG‑GGGGGGGGGGGGGGGGGGGGGGT‑GGGCGCGGGGGCGGCTGGGGTTGGTGGGGGGTGGGCGGCCTGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTaa < 7:243963/132‑1 (MQ=255) ggtcATTTTGCCCGG‑GGGGGGGGGGGGGGGGGGGGCGT‑TGGCGCGGGGGCCGCTGTGGTTTGGGGTGGGTGGGCGGCCTGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTaa < 7:10958/134‑1 (MQ=255) tgaTTTTGGCC‑TGGGGGGGGGGGGGGGGGGGGGGGT‑GGGCGCGGTGGCGGCTGGGGTTGGTGGTGGGGGGGCGGCCTGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAAc < 5:198142/135‑1 (MQ=255) gaTTTTGGCC‑T‑TGGGGTGGGTTTGTGGGGGGGGTGTGGCGCTGTGGCCGGTGGGGTTTTTTGTGGGTGGTGGGCGTTGGTAAGGGGCTGTAGCTCGATAATCGGTATTGCGGGTGCCGGCCTCTTCGCTGGTAAAcc < 8:279906/136‑1 (MQ=255) gCCC‑T‑GGGGGGGGGGGGGACGGGGGCGT‑TGGCGCGGTGGCCGCGGGGGTTTGTGGTTGGTGGGCGGCCTGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCt < 6:88027/137‑1 (MQ=38) gggggggggggggggggT‑GGGCGCTGTGGCCGCTGGGGTTTGTGGGGGGGGGGCGGCCTGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACACAGGgcc < 6:301781/136‑1 (MQ=38) ggggggggGT‑TGGCGGTGGGGCGGGGGGGGTTTGGGGGGGGGGGGCGGCCTGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACACAGGGCCGCGCCGCt < 1:202325/135‑1 (MQ=37) tgggT‑TGGCGCTGTGGCGGCTGGGGTTTGTGGTGGGGGGGCGGCCTGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACACAGGGCCGCGCCGCTTTGTa < 5:45454/134‑1 (MQ=255) | ACGATTGCCTTGCTGGGGTTTGGCGGAGCGCTGCTGGCGATGATTGGCGCCGGTGCCAGCTTGCTGCATTTTGCCC‑T‑GGCGGCGGGGGTGGCGGGGGCGT‑TGGCGCTGTGGCCGCTGTGGTTTGTGGTGGGTGGGCGGCCTGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACACAGGGCCGCGCCGCTTTGTA > ACB143/1537333‑1537564 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |