| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | ACB143 | 120,975 | Δ1 bp | 100% | intergenic (+272/+100) | polA → / ← engB | DNA polymerase I/putative GTP‑binding protein EngB |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | ACB143 | 120,975 | 0 | T | . | 96.9% | 285.8 / ‑1.1 | 64 | intergenic (+272/+100) | polA/engB | DNA polymerase I/putative GTP‑binding protein EngB |
| Reads supporting (aligned to +/- strand): ref base T (0/1); major base . (39/23); minor base G (0/1); total (39/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.81e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
| Rejected as polymorphism: Polymorphic indel expands or contracts a homopolymer stretch. | |||||||||||
GTAACTGAATGGCTGGTGAAGCCCTTCGATGCTCCTATGTTGTGTTAAGTGTTGGCAGATATCTGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGGTTTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGTCGAATACACCGCCGCATTCG‑CGGGTAAACCCGGGTCAGGCGCAAACCACTAAACGACCGGCTTGTCTTCCAGTTCCATCCA > ACB143/120847‑121107 | gTAACTGAATGGCTGGTGAAGCCCTTCGATGCTCCTATGTTGTGTTAAGTGTTGGCAGATATCTGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGGtttttttt < 7:251283/136‑1 (MQ=255)gTAACTGAATGGCTGGTGAAGCCCTTCGATGCTCCTATGTTGTGTTAAGGGTTGGCAGAAATCTGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGGtttttttt < 3:49314/136‑1 (MQ=255) aaCTGAATGGCTGGTGAAGCCCTTCGATGCTCCTATGTTGTGTTAAGTGTTGGCAGATATCTGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGCACTTCCCCCTCCCCATACGAAGTCCGGGGttttttttgc > 3:59421/1‑134 (MQ=255) aTGCTCCTATGTTGTGTTAAGTGTTGGCAGATATCTGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGG‑TTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTtc > 1:26612/1‑136 (MQ=255) tATGTTGTGTTAAGTGTTGGCAGATATCTGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGG‑TTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGaa > 7:129914/1‑136 (MQ=255) tgttgtGTTAAGTGTTGGCAGATATCTGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGG‑TTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAAt < 8:277084/135‑1 (MQ=255) tgttgtGTTAAGTGTTGGCAGATATCTGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGG‑TTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAAt < 1:170905/135‑1 (MQ=255) tgttgtGTTAAGTGTTGGCAGATATCTGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGG‑TTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAAt < 4:243774/135‑1 (MQ=255) tgttgtGTTAAGTGTTGGCAGATATCTGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGG‑TTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAAt < 3:96426/135‑1 (MQ=255) tgttgtGTTAAGTGTTGGCAGATATCTGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGG‑TTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAAt < 5:123399/135‑1 (MQ=255) tgttgtGTTAAGTGTTGGCAGATATCTGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCAGGGG‑TTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATg > 3:287992/1‑136 (MQ=255) ttgtGTTAAGTGTTGGCAGATATCTGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGG‑TTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGc < 2:262201/135‑1 (MQ=255) ttggGTTAAGTGTTGGCAGATATCTCGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGG‑TTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCg < 2:135977/136‑1 (MQ=255) tgtTAAGTGTTGGCAGATATCTGGACCCCGCCCTAGCTGTCCGGACTTGGTCCCCGAACTTCGCCCTCCCCATACGAAGTCC‑GGGTTTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGAt < 6:109682/135‑1 (MQ=255) tAAGTGTTGGCAGATATCTGGCCCCCGCCCTAGCGCTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGG‑TTTTTATTGCCAGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGTc > 4:227781/1‑135 (MQ=255) tAAGTGTTGGCAGATATCTGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATTCGAAGTCCGGGG‑TTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGTCg > 6:277606/1‑136 (MQ=255) tAAGTGTTGGCAGATATCTGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGG‑TTTTTTTTGCCCGTAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGTc > 2:62966/1‑135 (MQ=255) tAAGTGTTGGCAGATATCTGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGG‑TTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGTc > 7:22149/1‑135 (MQ=255) tAAGTGTTGGCAGATATCTGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGG‑TTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGTc > 3:86317/1‑135 (MQ=255) tAAGTGTTGGCAGATATCTGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGG‑TTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGTc > 1:186433/1‑135 (MQ=255) tAAGTGTTGGCAGATATCTGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGG‑TTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGTc > 1:123696/1‑135 (MQ=255) tAAGTGTTGGCAGATATCTGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGG‑TGTTTTTTGCCCGGAATTTGCCGGGCGCGGGTTTTCTCGCGAATGCGATGTc > 8:111603/1‑135 (MQ=255) aGTGTTGGCAGATATCTGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGG‑TTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGTCGaa > 6:272384/1‑136 (MQ=255) aGTGTTGGCAGATATCTGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGG‑TTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGTCGaa > 7:171543/1‑136 (MQ=255) aGTGTTGGCAGATATCTGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGG‑TTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCGCGCGAATGCGATGTCGaa > 7:191127/1‑136 (MQ=255) aGTGTTGGCAGATATCTGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGG‑TTTTTTTTGCCAGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGTCGaa > 1:87347/1‑136 (MQ=255) gtTGGCAGATATCTGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGG‑TTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGTCGAATa > 6:223854/1‑135 (MQ=255) gtTGGCAGATATCTGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGG‑TTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGTCGAATa > 8:61548/1‑135 (MQ=255) gtTGGCAGATATCTGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGG‑TTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGTCGAATa > 3:34876/1‑135 (MQ=255) cAGATATCTGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGG‑TTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGTCGAATACAccgc > 7:260784/1‑136 (MQ=255) cAGATATCTGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGG‑TTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGTCGAATACAccgc > 7:22613/1‑136 (MQ=255) cAGATATCTGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGG‑TTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGTCGAATACAccgc > 7:172506/1‑136 (MQ=255) cAGATATCTGGACCCCGCCCTAGCGGTCCGGACTTGGACACCGAACTTCCCCCTCCCCATACGAAGTCCGGGG‑TTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGTCGAATACAacgc > 7:188541/1‑136 (MQ=255) tCTGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGG‑TTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGTCGAATACACCGCCGCAt < 4:272818/135‑1 (MQ=255) tCTGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGG‑TTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGTCGAATACACCGCCGCAt < 3:137520/135‑1 (MQ=255) tGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCAATACGAAGACCGGGG‑TTTTTTTTGCCCGGAATTTGCCGGGAGCCGGTTTTCTCGCGAAAGCGATGTCGAATACACCGCCCCATTcg > 1:12609/1‑136 (MQ=255) aCCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGG‑TTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGTCGAATACACCGCCGCATTCG‑Cgg < 7:225126/136‑1 (MQ=255) ccTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGG‑TTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTcg > 3:283972/1‑94 (MQ=255) ccTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGG‑TTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTcg < 4:283972/94‑1 (MQ=255) ccTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGG‑TTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGTCGAATACACCGCCGCATTCG‑CGGGTAAccc > 2:183912/1‑136 (MQ=255) ccTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGG‑TTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGTCGAATACACCGCCGCATTCG‑CGGGTAAAcc > 8:240742/1‑136 (MQ=255) ccTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGG‑TTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGTCGAATACACCGCCGCATTCG‑CGGGTAAAcc > 1:48978/1‑136 (MQ=255) ccTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGG‑TTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGTCGAATACACCGCCGCATTCG‑CGGGTAAAcc > 4:274051/1‑136 (MQ=255) ccTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGG‑TTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGTCGAATACACCGCCGCATTCG‑CGGGTAAAcc > 6:81397/1‑136 (MQ=255) cTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCAATACGAAGTCCGGGG‑TTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGTCGAATACACCGCCGCATTCG‑CGGGTAAAccc > 3:248437/1‑136 (MQ=255) gCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGG‑TTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGTCGAATACACCGCCGCATTCG‑CGGGTAAACCCgg < 6:202334/135‑1 (MQ=255) gCGGGCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGG‑TTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGTCGAATACACCGCCGCATTCG‑CGGGTAAACCCg < 5:85140/134‑1 (MQ=255) cGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGG‑TTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGTCGAATACACCGCCGCATTCG‑CGGGTAAACCCGGGTCAGgc < 1:212787/136‑1 (MQ=255) cGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGG‑TTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGTCGAATACACCGCCGCATTCG‑CGGGTAAACCCGGGTCAGgc < 7:33220/136‑1 (MQ=255) aCTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGG‑TTTTTTTTGCCCGGAATTTGCGGGGACCGGGTTTACACGCGAATGCGTTGGCGAATACACCGCCGCATACG‑CGGGTAACCCCGGGTCAGgcga > 5:208725/1‑134 (MQ=255) aCTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCAGGGG‑ATTTTTTTGCCCGGCATTAGCCGGGATCTGGTTTTCTCCCGAATGCGAGGTCGAATACACCTACGCAGTCG‑CGGGTAAACCCGCGTCAGgcgc > 7:94004/1‑135 (MQ=255) cccGAACTTCCCCCTCCCCATACGAAGTCCGGGG‑TTTTTTTTGCCGGTAATTTGCGGGGCGCGGGTTTGATCGCGATTGGGTTGTCGAA‑AAACCCCCCCCTTAGCCGGGTAACCCCGGGGCAGGCGCAAAcacca > 2:124456/1‑131 (MQ=255) cccGAACTTCCCCCTCCCCATACGAAGTCCGGGG‑TTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGTCGAATACACCGCCGCATTCG‑CGGGTAAACCCGGGTCAGGCGCAAACCACt > 4:224614/1‑135 (MQ=255) aCTTCCCCCTCCGATTCCTATGTCGGGTG‑TTTTTTTTGCCGTTTATTTGCCGGGAGGGGGTTTTCTCGCTATTGGGTTGTGGAATACACCGCGGCTTTGG‑CGGGTAAACCCGGGTCAGGGGAAAACCACTAAACGa < 4:88151/136‑1 (MQ=255) aCTTCCCCCTCCCCATACGAAGTCCGGGG‑TTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAAt < 7:124964/73‑1 (MQ=255) aCTTCCCCCTCCCCATACGAAGTCCGGGG‑TTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAAt > 8:124964/1‑73 (MQ=255) aCTTCCCCCTCCCCATACGAAGTCCGGGG‑TTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGTCGAATACACCGCCGCATTCG‑CGGGTAAACCCGGGTCAGGCGCAAACCACTAAACGa > 8:274404/1‑136 (MQ=255) cccccTCCCCATACGAAGTCCGGGG‑TTTTTTTTGCCCGTAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGTCGAATACACCGCCGCATTCG‑CGGGTAAACCCGGGTCAGGCGCAAACCACTAAACGAcc < 1:133056/134‑1 (MQ=255) ccccTCCCCATACTAAGTCCGGGG‑TTTTTTTTGCCCGTAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCTATGTCGAATACACCGCCGCATTCG‑CGGGTAAACCCGGGTCAGGCGCAAACCACTAAACGACCGGc < 8:117988/136‑1 (MQ=39) ccccATACTAAGTCCGGGG‑TTTTTTTTGCCCGTAATTTGCCGGGAGCGGGTTTTCTCGCTAATGCGATGTCTAATACACCGCCGCATTCG‑CGGGTAAACCCGGGTCAGGCGCAAACCACTAAACGACCGGCTTGTc < 4:267383/136‑1 (MQ=255) ccccATACGACGTCCGGGG‑TTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGTCGAATACACCGCCGCATTCG‑CGGGTAAACCCGGGTCAGGCGCAAACCACTAAACGACCGGCTTGTc < 8:38100/136‑1 (MQ=255) cccATACGAAGTCCGGGG‑TTTTTTTTGCCGGTAATTTGCCGGGAGCGGGTTTTCTCGCTAATGCTATGTCTAATACACCGCCGCATTCG‑CGGTTAAACCCGGGTCAGGCGCAAACCACTAAACGACCGGCTTGTc < 8:179867/135‑1 (MQ=255) cccATACGAAGTCCGGGG‑TTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGTCGAATACACCGCCGCATTCG‑CGGGTAAACCCGGGTCAGGCGCAAACCACTAAACGACCGGCTTGTc < 8:183742/135‑1 (MQ=255) cATACGAAGTCCGGGG‑TTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGTCGAATACACCGCCGCATTCG‑CGGGTAAACCCGGGTCAGGCGCAAACCACTAAACGACCGGCTTGTCTTc > 2:44058/1‑136 (MQ=255) aTACGAAGTCCGGGG‑TTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGTCGAATACACCGCCGCATTCG‑CGGGTAAACCCGGGTCAGGCGCAAACCACTAAACGACCGGCTTGTCTTc > 3:287635/1‑135 (MQ=255) aTACGAAGTCCGGGG‑TTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGTCGAATACACCGCCGCATTCG‑CGGGTAAACACGGGTCAGGCGCAAACCACTAAACGACCGGCTTGTCTTc > 6:167797/1‑135 (MQ=255) ggggGTTTTTTTTGCCCGGAATTTGCCGGTAGCGGGTTTTCGCGCGAATGCTATGTCGATTACACAGCCGCATTCG‑CGGGTAAACCCGGGTCAGGCGCAAACCCCTAAACGACCGGCTTGTCTTCCAGTtccatc < 8:179311/135‑1 (MQ=255) cggggTTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGTCGAATACACCGCCGCATTCG‑CGGGTAAACCCGGGTCAGGCGCAAACCACTAAACGACCGGCTTGTCTTCCAGTtccatc > 6:248993/2‑135 (MQ=255) cggggTTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGTCGAATACACCGCCGCATTCG‑CGGGTAAACCCGGGTCAGGCGCAAACCACTAAACGACCGGCTTGTCTTCCAGTtccatc > 2:8913/2‑135 (MQ=255) ggggTTTTTTTTGCCCGTAATTTGCCGGGAGCGGGTTTTCTCGCTAATGCGATGTCCAATACACCGCCGCATTCG‑CGGGTAAACCCGGGTCAGGCGCAAACCACAAAACGACCGGCTTGTCTTCCAGTtccatcca < 4:63436/136‑1 (MQ=255) | GTAACTGAATGGCTGGTGAAGCCCTTCGATGCTCCTATGTTGTGTTAAGTGTTGGCAGATATCTGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGGTTTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGTCGAATACACCGCCGCATTCG‑CGGGTAAACCCGGGTCAGGCGCAAACCACTAAACGACCGGCTTGTCTTCCAGTTCCATCCA > ACB143/120847‑121107 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |