Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB143 733,847 C→T 92.3% intergenic (‑171/‑46) epsF ← / → pilA Type II secretion system protein F/Fimbrial protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB143733,8470CT92.3% 160.7 / 2.9 52intergenic (‑171/‑46)epsF/pilAType II secretion system protein F/Fimbrial protein
Reads supporting (aligned to +/- strand):  ref base C (2/2);  new base T (34/14);  total (36/16)
Fisher's exact test for biased strand distribution p-value = 5.78e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AACAGCTTGCCGCGTCCATGCAAGGGCACGCGTCCGTGATGCTCACTAGAGTAGTTCAGCGAAGATGACGCCCGTGCGGGCAGGGGTGACAAAAGGTGTCTGTTCAGGCCTACTGCGCCGCTGCCGGTTGGGGCGCAACTGACCTCAATGTGTAAGCGAATCAAAAAGGAACTACGTTCATGAAAGGGCAACGCGGTATCACCCTGATCGAACTGATGATCGTCGTCGCGATCATCGGCATTCTGGCGACCATCGCCTTACC  >  ACB143/733714‑733975
                                                                                                                                     |                                                                                                                                
aaCAGCTTGCCGGGGCCAGGCAAGGGCACGCGTCCGTGATGCTCACTAGAGTAGTTCAGCGAAGATGACGCCCGTGCGGGCAGGGGTGACAAAAGGTGTCTGTTCAGGCCTACTGCGCCGCTGCCGGTTGGGgcgc                                                                                                                                <  2:95333/136‑1 (MQ=255)
aaCAGCTTGCCGCGTCCATGCAAGGGCACGCGTCCGTGATGCTCACTAGAGTAGTTCAGCGAAGATGACGCCCGTGCGGGCAGGGGTGACAAAAGGTGTCTGTTCAGGCCTACTGCGCCGCTGCCGGTTGGGgtgc                                                                                                                                <  3:259234/136‑1 (MQ=255)
aaCAGCTTGCCGCGTCCATGCAAGGGCACGCGTCCGTGATGCTCACTAGAGTAGTTCAGCGAAGATGACGCCCGTGCGGG‑AGGGGTGACAAAAGGTGTCTGTTCAGGCCTACTGCGCCGCTGCCGGTTGGGgtgc                                                                                                                                <  1:102532/135‑1 (MQ=255)
    gCTTGCCGCGTCCATGCAAGGGCACGCGTCCGTGATGCTCACTAGAGTAGTTCAGCGAAGATGACGCCCGTGCGGGCAGGGGTGACAAAAGGTGTCTGTTCAGGCCTACTGCGCCGCTGCCGGTTGGGGCGCAACt                                                                                                                            <  7:162566/136‑1 (MQ=255)
                 aTGCAAGGGCACGCGTCCGTGATGCTCACTAGAGTAGTTCAGCGAAGATGACGCCCGTGCGGGCAGGGGTGACAAAAGGTGTCTGTTCAGGCCTACTGCGCCGCTGCCGGTTGGGGTGCAACTGACCTCAATgtgt                                                                                                               <  6:262619/136‑1 (MQ=255)
                          cACGCGTCCGTGATGCTCACTAGAGTAGTTCAGCGAAGATGACGCCCGTGCGGGCAGGGGTGACAAAAGGTGTCTGTTCAGGCCTACTGCGCCGCTGCCGGTTGGGGTGCAACTGACCTCAATGTGTAAGCGAATc                                                                                                      <  3:71267/136‑1 (MQ=255)
                                  cGTGATGCTCACTAGAGTAGTTCAGCTAATCTGACGCCCGTGCGGGCAGGGGTGACAAAAGGTGTCTGTTCAGGCCTACTGCGCCGCTGCCGGTTGGGGTGCAACTGACCTCAATGTGTAAGCGAATCAAAAAGGa                                                                                              <  4:63046/136‑1 (MQ=255)
                                       tGCTCACTAGAGTAGGTCAGCGAAGATGACGCCCGTGCGGGCAGGGGTGACAAAAGGTGTCTGTTCAGGCCTACTGCGCCGCTGCCGGTTGGGGTGCAACTGACCTCAATGTGTAAGCGAATCAAAAAGGAACTAc                                                                                         <  4:20591/136‑1 (MQ=255)
                                            aCTAGAGTAGTTCAGCGAAGATGACGCCCGTGCGGGCAGGGGTGACAAAAGGTGTCTGTTCAGGCCTACTGCGCCGCTGCCGGTTGGGGTGCAACTGACCTCAATGTGTAAGCGAATCAAAAAGGAACTACGTTCa                                                                                    >  3:159643/1‑136 (MQ=255)
                                              tAGAGTAGTTCAGCGAAGATGACGCCCGTGCGGGCAGGGGTGACAAAAGGTGTCTGTTCAGGCCTACTGCGCCGCTGCCGGTTGGGGTGCAACTGACCTCAATGTGTAAGCGAATCAAAAAGGAACTACGTTCAt                                                                                   >  3:265149/1‑135 (MQ=255)
                                                    agtTCAGCGAAGATGACGCCCGTGCGGGCAGGGGTGACAAAAGGTGTCTGTTCAGGCCTACTGCGCCGCTGCCGGTTGGGGTGCAACTGACCTCAATGTGTAAGCGAATCAAAAAGGAACTACGTTCATGAAAggg                                                                            >  3:39263/1‑136 (MQ=255)
                                                    agtTCAGCGAAGATGACGCCCGTGCGGGCAGGGGTGACAAAAGGTGTCTGTTCAGGCCTACTGCGCCGCTGCCGGTTGGGGCGCAACTGACCTCAATGTGTAAGCGAATCAAAAAGGAACTACGTTCATGAAAggg                                                                            >  2:113156/1‑136 (MQ=255)
                                                    agtTCAGCGAAGATGACGCCCGTGCGGGCAGGGGTGACAAAAGGTGTCTGTTCAGGCCTACTGCGCCGCTGCCGGTTGGGGCGCAACTGACCTCAATGTGTAAGCGAATCAAAAAGGAACTACGTTCATGAAAggg                                                                            >  2:117347/1‑136 (MQ=255)
                                                      ttCAGCGAAGATGACGCCCGTGCGGGCAGGGGTGACAAAAGGTGTCTGTTCAGGCCTACTGCGCCGCTGCCGGTTGGGGTGCAACTGACCTCAATGTGTAAGCGAATCAAAAAGGAACTACGTTCATGAAAGGGCa                                                                          <  1:190813/136‑1 (MQ=255)
                                                      ttCAGCGAAGATGACGCCCGTGCGGGCAGGGGTGACAAAAGGTGTCTGTTCAGGCCTACTGCGCCGCTGCCGGTTGGGGTGCAACTGACCTCAATGTGTAAGCGAATCAAAAAGGAACTACGTTCATGAAAGGGCa                                                                          <  4:42171/136‑1 (MQ=255)
                                                      ttCAGCGAAGATGACGCCCGTGCGGGCAGGGGTGACAAAAGGTGTCTGTTCAGGCCTACTGCGCCGCTGCCGGTTGGGGTGCAACTGACCTCAATGTGTAAGCGAATCAAAAAGGAACTACGTTCATGAAAGGGCa                                                                          <  1:215761/136‑1 (MQ=255)
                                                         aGCGAAGATGACGCCCGTGCGGGCAGGGGTGACAAAAGGTGTCTGTTCAGGCCTACTGCGCCGCTGCCGGTTGGGGTGCATCTGACCTCAATGTGTAAGCGAAGCAAAAAGGAACGACGTTCATGAAAGGGCAAcg                                                                       >  4:7595/1‑136 (MQ=255)
                                                         aGCGAAGATGACGCCCGTGCGGGCAGGGGTGACAAAAGGTGTCTGTTCAGGCCTACTGCGCCGCTGCCGGTTGGGGTGCAACTGCCCTCAATGTGTAAGCGAATCAAAAAGGAACTACGTTCATGAAAGGGCAAcg                                                                       >  5:195945/1‑136 (MQ=255)
                                                         aGCGAAGATGACGCCCGTGCGGGCAGGGGTGACAAAAGGTGTCTGTTCAGGCCTACTGCGCCGCTGCCGGTTGGGGTGCAACTGACCTCAATGTGTAAGCGAATCAAAAAGGAACTACGTTCATGAAAGGGCACcg                                                                       >  6:12277/1‑136 (MQ=255)
                                                         aGCGAAGATGACGCCCGTGCGGGCAGGGGTGACAAAAGGTGTCTGTTCAGGCCTACTGCGCCGCTGCCGGTTGGGGTGCAACTGACCTCAATGTGTAAGCGAATCAAAAAGGAACTACGTTCATGAAAGGGCAAcg                                                                       >  3:13220/1‑136 (MQ=255)
                                                         aGCGAAGATGACGCCCGTGCGGGCAGGGGTGACAAAAGGTGTCTGTTCAGGCCTACTGCGCCGCTGCCGGTTGGGGTGCAACTGACCTCAATGTGTAAGCGAATCAAAAAGGAACTACGTTCATGAAAGGGCAAcg                                                                       >  2:51112/1‑136 (MQ=255)
                                                         aGCGAAGATGACGCCCGTGCGGGCAGGGGTGACAAAAGGTGTCTGTTCAGGCCTACTGCGCCGCTGCCGGTTGGGGTGCAACTGACCTCAATGTGTAAGCGAATCAAAAAGGAACTACGTTCATGAAAGGGCAAcg                                                                       >  5:33358/1‑136 (MQ=255)
                                                         aGCGAAGATGACGCCCGTGCGGGCAGGGGTGACAAAAGGTGTCTGTTCAGGCCTACTGCGCCGCTGCCGGTTGGGGTGCAACTGACCTCAATGTGTAAGCGAATCAAAAAGGAACTACGTTCATGAAAGGGCAAcg                                                                       >  3:253270/1‑136 (MQ=255)
                                                         aGCGAAGATGACGCCCGTGCGGGCAGGGGTGACAAAAGGTGTCTGTTCAGGCCTACTGCGCCGCTGCCGGTTGGGGTGCAACTGACCTCAATGTGTAAGCGAATCAAAAAGGAACTACGTTCATGAAAGGGCAAcg                                                                       >  2:131492/1‑136 (MQ=255)
                                                         aGCGAAGATGACGCCCGTGCGGGCAGGGGTGACAAAAGGTGTCTGTTCAGGCCTACTGCGCCGCTGCCGGTTGGGGTGCAACTGACCTCAATGTGTAAGCGAATCAAAAAGGAACTACGTTCATGAAAGGGCAAcg                                                                       >  4:97525/1‑136 (MQ=255)
                                                         aGCGAAGATGACGCCCGTGCGGGCAGGGGTGACAAAAGGTGTCTGTTCAGGCCTACTGCGCCGCTGCCGGTTGGGGTGCAACTGACCTCAATGTGTAAGCGAATCAAAAAGGAACTACGTTCATGAAAGGGCAAcg                                                                       >  3:9331/1‑136 (MQ=255)
                                                         aGCGAAGATGACGCCCGTGCGGGCAGGGGAGACAAAAGGTGTCTGTTCAGGCCTACTGCGCCGCTGCCGGTTGGGGTGCAACTGACCTCAATGTGTAAGCGAATCAAAAAGGAACTACGTTCATGAAACGACAAcg                                                                       >  2:206586/1‑136 (MQ=255)
                                                           cGAAGATGACGCCCGTGCGGGCAGGGGTGACAAAAGGTGTCTGTTCAGGCCTACTGCGCCGCTGCCGGTTGGGGTGCAACTGACCTCAATGTGTAAGCGAATCAAAAAGGAACTACGTTCATGAAAGGGCACcgcg                                                                     >  1:282250/1‑136 (MQ=255)
                                                           cGAAGATGACGCCCGTGCGGGCAGGGGTGACAAAAGGTGTCTGTTCAGGCCTACTGCGCCGCTGCCGGTTGGGGTGCAACTGACCTCAATGTGTAAGCGAATCAAAAAGGAACTACGTTCATGAAAGGGCAAcgcg                                                                     >  7:198789/1‑136 (MQ=255)
                                                           cGAAGATGACGCCCGTGCGGGCAGGGGTGACAAAAGGTGTCTGTTCAGGCCTACTGCGCCGCTGCCGGTTGGGGTGCAACTGACCTCAATGTGTAAGCGAATCAAAAAGGAACTACGTTCATGAAAGGGCAAcgcg                                                                     <  6:29058/136‑1 (MQ=255)
                                                           cGAAGATGACGCCCGTGCGGGCAGGGGTGACAAAAGGTGTCTGTTCAGGCCTACTGCGCCGCTGCCGGTTGGGGTGCAACTGACCTCAATGTGTAAGCGAATCAAAAAGGAACTACGTTCATGAAAGGGCAAcgcg                                                                     >  6:209084/1‑136 (MQ=255)
                                                              aGATGACGCCCGTGCGGGCAGGGGTGACAAAAGGTGTCTGTTCAGGCCTACTGCGCCGCTGCCGGTTGGGGTGCAACTGACCTCAATGTGTAAGCGAATCAAAAAGGAACTACGTTCATGAAAGGGCAACGCGGTa                                                                  >  1:164573/1‑136 (MQ=255)
                                                                   aCGCCCGTGCGGGCAGGGGTGACAAAAGGTGTCTGTTCAGGCCTACTGCGCCGCTGCCGGTTGGGGTGCAACTGACCTCAATGTGTAAGCGAATCAAAAAGGAACTACGTTCa                                                                                    <  1:125392/113‑1 (MQ=255)
                                                                   aCGCCCGTGCGGGCAGGGGTGACAAAAGGTGTCTGTTCAGGCCTACTGCGCCGCTGCCGGTTGGGGTGCAACTGACCTCAATGTGTAAGCGAATCAAAAAGGAACTACGTTCa                                                                                    >  2:125392/1‑113 (MQ=255)
                                                                         gTGCGGGCAGGGGTGACAAAAGGTGTCTGTTCAGGCCTACTGCGCCGCTGCCGGTTGGGGTGCAACTGACCTCAATGTGTAAGCGAATCAAAAAGGAACTACGTTCATGAAAGGGCAACGCGGTATCACCCTGATc                                                       <  1:131492/136‑1 (MQ=255)
                                                                                      tGACAAAAGGTGTCTGTTCAGGCCTACTGCGCCGCTGCCGGTTGGGGTGCAACTGACCTCAATGTGTAAGCGAATCAAAAAGGAACTACGTTCATGAAAGGGCAACGCGGTATCACCCTGATCGAACTGATGAtcg                                          >  6:149986/1‑136 (MQ=255)
                                                                                         cAAAAGGTGTCTGTTCAGGCCTACTGCGCCGCTGCCGGTTGGGGCGCAACTGACCTCAATGTGTAAGCGAATCAAAAAGGAACTACGTTCATGAAAGGGCAACGCGGTATCACCCTGATCGAACTGATGAtcgtcg                                       <  3:180478/136‑1 (MQ=255)
                                                                                           aaaGGTGTCTGTTCAGGCCTACTGCGCCGCTGCCGGTTGGGGTGCAACTGACCTCAATGTGTAAGCGAATCAAAAAGGAACTACGTTCATGAAAGGGCAACGCGGTATCACCCTGATCGAACTGATGAtcgtcgtc                                     >  2:78783/1‑136 (MQ=255)
                                                                                           aaaGGTGTCTGTTCAGGCCTACTGCGCCGCTGCCGGTTGGGGTGCAACTGACCTCAATGTGTAAGCGAATCAAAAAGGAACTACGTTCATGAAAGGGCAACGCGGTATCACCCTGATCGAACTGATGAtcgtcgtc                                     >  6:271599/1‑136 (MQ=255)
                                                                                                     gTTCAGGCCTACTGCGCCGCTGCCGGTTGGGGTGCAACTGACCTCAATGTGTAAGCGAATCAAAAAGGAACTACGTTCATGAAAGGGCAACGCGGTATCACCCTGATCGAACTGATGATCGTCGTCGCGATCATCg                           >  3:123489/1‑136 (MQ=255)
                                                                                                         aGGCCTACTGCGCCGCTGCCGGTTGGGGTGCAACTGACCTCAATGTGTAAGCGAATCAAAAAGGAACTACGTTCATGAAAGGGCAACGCGGTATCACCCTGATCGAACTGATGATCGTCGTCGCGATCATCGGCAt                       >  5:6020/1‑136 (MQ=255)
                                                                                                             cTACTGCGCCGCTGCCGGTTGGGGTGCAACTGACCTCAATGTGTAAGCGAATCAAAAAGGAACTACGTTCATGAAAGGGCAACGCGGTATCACCCTGATCGAACTGATGATCGTCGTCGCGATCATCGGCATTCTg                   >  4:288622/1‑136 (MQ=255)
                                                                                                             cTACTGCGCCGCTGCCGGGTGGGGTGCAACTGACCTCAATGTGTAAGCGAATCAAAAAGGAACTACGTTCATGAAAGGGCAACGCGGTATCACCCTGATCGAACTGATGATCGTCGTCGCGATCATCGGCATTCTg                   >  6:185001/1‑136 (MQ=255)
                                                                                                              tACTGCGCCGCTGCCGGTTGGGGTGCAACTGACCTCAATGTGTAAGCGAATCAAAAAGGAACTACGTTCATGAAAGGGCAACGCGGTATCACCCTGATCGAACTGATGATCGTCGTCGCGATCATCGGCATTCTgg                  >  7:54031/1‑136 (MQ=255)
                                                                                                              tACTGCGCCGCTGCCGGTTGGGGTGCAACTGACCTCAATGTGTAAGCGAATCAAAAAGGAACTACGTTCATGAAAGGGCAACGCGGTATCACCCTGATCGAACTGATGATCGTCGTCGCGATCATCGGCATTCTgg                  >  1:236248/1‑136 (MQ=255)
                                                                                                              tACTGCGCCGCTGCCGGTTGGGGTGCAACTGACCTCAATGTGTAAGCGAATCAAAAAGGAACTACGATCATGAAAGGGCAACGCGGTATCACCCTGAGCGAACTGATGATCGTCGTCGCGATCATCGGCAGTCTgg                  >  6:133853/1‑136 (MQ=255)
                                                                                                                 tGCGCCGCTGCCGGTTGGGGTGCAACTGACCTCAATGTGTAAGCGAATCAAAAAGGAACTACGTTCATGAAAGGGCAACGCGGTATCACCCTGATCGAACTGATGATCGTCGTCGCGATCATCGGCATTCTGGCGa               >  6:9599/1‑136 (MQ=255)
                                                                                                                        cTGCCGGTTGGGGTGCAACTGACCTCAATGTGTAAGCGAATCAAAAAGGAACTACGTTCATGAAAGGGCAACGCGGTATCACCCTGATCGAACTGATGATCGTCGTCGCGATCATCGGCATTCTGGCGACCATCGc        >  8:69572/1‑136 (MQ=255)
                                                                                                                        cTGCCGGTTGGGGTGCAACTGACCTCAATGTGTAAGCGAATCAAAAAGGAACTACGTTCATGAAAGGGCAACGCGGTATCACCCTGATCGAACTGATGATCGTCGTCGCGATCATCGGCATTCTGGCGACCATCGc        >  7:251959/1‑136 (MQ=255)
                                                                                                                           ccGGTTGGTGTGCAACTGACCTCAATGTGTAAGCGAATCAAAAAGGAACTACGTTCATGAAAGGGCAACGCGGTATCACCCTGATCGAACTGATGATCGTCGTCGCGATCATCGGCATTCTGGCGACCATCGcc       <  8:197597/134‑1 (MQ=255)
                                                                                                                           ccGGTTGGGGTGCAACTGACCTCAATGTGTAAGCGAATCAAAAAGGAACTACGTTCATGAAAGGGCAACGCGGTATCACCCTGATCGAACTGATGATCGTCGTCGCGATCATCGGCATTCTGGCGACCATCGcc       <  7:140992/134‑1 (MQ=255)
                                                                                                                           ccGGTTGGGGTGCAACTGACCTCAATGTGTAAGCGAATCAAAAAGGAACTACGTTCATGAAAGGGCAACGCGGTATCACCCTGATCGAACTGATGATCGTCGTCGCGATCATCGGCATTCTGGCGACCATCGCCt      >  8:20593/1‑135 (MQ=255)
                                                                                                                           ccGGTTGGGGTGCAACTGACCTCAATGTGTAAGCGAATCAAAAAGGAACTACGTTCATGAAAGGGCAACGCGGTATCACCCTGATCGAACTGATGATCGTCGTCGCGATCATCGGCATTCTGGCGACCATCGCCt      >  4:206965/1‑135 (MQ=255)
                                                                                                                            cGGTTGGGGTGCAACTGACCTCAATGTGTAAGCGAATCAAAAAGGAACTACGTTCATGAAAGGGCAACGCGGTATCACCCTGATCGAACTGATGATCGTCGTCGCGATCATCGGCATTCTGGCGACCATCGCCTTa    <  1:54344/136‑1 (MQ=255)
                                                                                                                              gTTGGGGTGCAACTGACCTCAATGTGTAAGCGAATCAAAAAGGAACTACGTTCATGAAAGGGCAACGCGGTATCACCCTGATCGAACTGATGATCGTCGTCGCGATCATCGGCATTCTGGCGACCATCGCCTTAcc  <  3:193163/136‑1 (MQ=255)
                                                                                                                                     |                                                                                                                                
AACAGCTTGCCGCGTCCATGCAAGGGCACGCGTCCGTGATGCTCACTAGAGTAGTTCAGCGAAGATGACGCCCGTGCGGGCAGGGGTGACAAAAGGTGTCTGTTCAGGCCTACTGCGCCGCTGCCGGTTGGGGCGCAACTGACCTCAATGTGTAAGCGAATCAAAAAGGAACTACGTTCATGAAAGGGCAACGCGGTATCACCCTGATCGAACTGATGATCGTCGTCGCGATCATCGGCATTCTGGCGACCATCGCCTTACC  >  ACB143/733714‑733975

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑