Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB143 2,171,266 G→C 13.8% A149P (GCA→CCA)  CJ019_02060 → IS1182 family transposase ISPpu16

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1432,171,2660GC13.8% 282.0 / 8.8 122A149P (GCA→CCA) CJ019_02060IS1182 family transposase ISPpu16
Reads supporting (aligned to +/- strand):  ref base G (59/43);  new base C (2/16);  total (62/60)
Fisher's exact test for biased strand distribution p-value = 2.31e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 122 total aligned reads displayed.

AAAACCATCGCCGACTTTCGCAAGGACAACGGTAAAGCCATTCGTAGCGTCTGCCGTCAGTTCGTGATCCTCTGCCGTAACCTCAACCTTTTCTCTGAGTCGATCATTG‑CCATCGACGGCAGCAAGTTCAAA‑GCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGATTCGGCAGACCGGGCAATGCCGGAGGTG  >  ACB143/2171134‑2171391
                                                                                                                                      |                                                                                                                             
aaaaCCATCGCCGACTTTCGCAAGGACAACGGTAAAGCCATTCGTAGCGTCTGCCGTCAGTTCGTGATCCTCTGCCGTAACCTCAACCTTTTCTCTGAGTCGATCATTG‑CCATCGACGGCAGCAAGTTCAAA‑GCAg                                                                                                                            <  5:126276/136‑1 (MQ=255)
       tCGCCGACTTTCGCAAGGACAACGGTAAAGCCATTCGTAGCGTCTGCCGTCAGTTCGTGATCCTCTGCCGTAACCTCAACCTTTTCTCTGAGTCGATCATTG‑CCATCGACGGCAGCAAGTTCAAA‑GCAGTGAACaa                                                                                                                     >  8:238579/1‑136 (MQ=255)
        cGCCGACTTTCGCAAGGACAACGGTAAAGCCATTCGTAGCGTCTGCCGTCAGTTCGTGATCCTCTGCCGTAACCTCAACCTTTTCTCTGAGTCGATCATTG‑CCATCGACGGCAGCAAGTTCAAA‑GCAGTGAACaa                                                                                                                     >  2:5723/1‑135 (MQ=255)
          ccGACTTTCGCAAGGACAACGGTAAAGCCATTCGTAGCGTCTGCCGTCAGTTCGTGATCCTCTGCCGTAACCTCAACCTTTTCTCTGAGTCGATCATTG‑CCATCGACGGCAGCAAGTTCAAA‑GCAGTGAACAATcg                                                                                                                  >  7:18508/1‑136 (MQ=255)
          ccGACTTTCGCAAGGACAACGGTAAAGCCATTCGTAGCGTCTGCCGTCAGTTCGTGATCCTCTGCCGTAACCTCAACCTTTTCTCTGAGTCGATCATTG‑CCATCGACGGCAGCAAGTTCAAA‑GCAGTGAACAATcg                                                                                                                  >  4:281511/1‑136 (MQ=255)
          ccGACTTTCGCAAGGACAACGGTAAAGCCATTCGTAGCGTCTGCCGTCAGTTCGTGATCCTCTGCCGAAACCTCAACCTTTTCTCTGAGTAGATCATTG‑CAATCGACGGCCGCAAGTTAAAA‑CCAGTGAACAATcg                                                                                                                  >  2:109428/1‑136 (MQ=255)
              cTTCCGAAAGCACAACGTAAAACCCATTCGTAGCTGCTGCCTTCAGTTCGTTATCCTCTGCCTAAACCACAACCTTTTCTCTGAGTCGATCATTG‑CCATCGACGGCAGCAAGTTCAAC‑GCAGTCAACAAGCGCGa                                                                                                               <  8:274384/135‑1 (MQ=255)
                   ccAAGGACACCGGAAAAGCCATTCGTAGCGTCTGCCGGCTGTTCGTTATCCTCTGCCTTAACCTCAACCTTTTCTCTGAGTCGATCATTG‑CCATCGAGGGCAGCAAGTTCAAA‑GCAGTTAACAATCGCGACCGTa                                                                                                          <  6:275806/134‑1 (MQ=255)
                       ggACAACGGTAAAGCAATTCGTAGCGTCTGCCGTCAGTTGGTGATCCTCTGCCGTAACCTAAACCTTTTCTCTGAGTCGATCATTG‑CCATCGACGGCAGCAAGTTCAAA‑GCAGTTAACAATCGCGACCGTAACTTc                                                                                                     <  7:213908/136‑1 (MQ=255)
                       ggAAAACGTTAAAGCCATTCGTCGCGGCTGCCGTCAGTTGGTGATCCTCTGCCGTAACCTCACCATTTTCTCTGATTCTATCATTG‑CCATCGACGGCAGCAAGTTCAAA‑GCAGTCAAAAATCGCGACCGTAACTTc                                                                                                     <  8:154423/136‑1 (MQ=255)
                              ttaaaaGCCATTCTTAGCTTCTGCAGTTTGTGCGTTATCTTAGCCCCAAACCAAAACCTTTTATCTTAGCCTATTATTG‑CCTTCGACGGCTG‑AATTTCAAATGCTGTGAACAATCGCGACCGTAACTTCACCCAgg                                                                                              <  2:32731/133‑1 (MQ=255)
                              gtaaaaGCATTTCGTAGCGTCTGCCTTCTGTTCGTTATCCTCTGCCTAAACCTAAACTTTTTCTGTGCGTCTATCATTG‑CCATCGAGGGAAGCAAGTTCAAC‑GCAGTTAACAAGCGCGACCGTAACTTCACCCAgg                                                                                              <  8:68002/133‑1 (MQ=255)
                              gtaaaaCCCATTCTTCGCGTCTGCCGTCATTTCGTGATCCTCTGCCGAAACCTAAACCTTTTCTCTGAGTCTATCATTC‑CAATCGACGGCAGAATTTTCAAA‑GCTGTAAAAAACCGCGACCGTAACTTCACCCAgg                                                                                              <  2:18784/133‑1 (MQ=255)
                                   cgCCATTCGTCGCGTCGCCCGGCTGTTCGTGATCCTCTGCCGAACCCACAACGTTTTCTCTGAGTCGATCTTTC‑CCCGCCAGGGCAGCAATTACAAA‑GCAGTCAACAACCGCGACCGTAACTTCACCCAGGCCAAg                                                                                         <  6:253444/135‑1 (MQ=255)
                                     ccATTCGTAGCGTCTGCCGTCAGTTCGTGATCCTCTGCCGTAACCTCAACCTTTTCTCTGAGTCGATCATTG‑CCATAGACGGCAGCAAGTTCAAA‑GCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGt                                                                                       >  1:245257/1‑136 (MQ=255)
                                      cATTCGTAGCGTCTGCCGTCAGTTCGTGATCCTCTGCCGTAACCTCAACCTTTTCTCTGAGTCGATCATTG‑CCATCGACGGCAGCAAGTTCAAA‑GCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGt                                                                                       >  3:291990/1‑135 (MQ=255)
                                           gTAGTGTCTGCCGTCAGTTCGTGATCCTCTGCCGTAACCTCAACCTTTTCTCTGAGTCGATCATTG‑CCATCGACGGCAGCAAGTTCAAA‑GCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGc                                                                                 >  7:134447/1‑136 (MQ=255)
                                           gTAGCGTCTGCCGTCAGTTCGTGATCCTCTGCCGTAACCTCAACCTTTTCTCTGAGTCGATCATTG‑CCATCGACGGCAGCAAGTTCAAA‑GCAGTGCACAATCGCGACCGTAACTTCACCCCGGCCAAGGTGAAGGc                                                                                 >  2:111223/1‑136 (MQ=255)
                                           gTAGCGTCTGCCGTCAGTTCGTGATCCTCTGCCGTAACCTCAACCTTTTCTCTGAGTCGATCATTG‑CCATCGACGGCAGCAAGTTCAAA‑GCAGTGAACAATCGCGACCGTAACTTCACCCCGGCCAAGGTGAAGGc                                                                                 >  2:108628/1‑136 (MQ=255)
                                           gTAGCGTCTGCCGTCAGTTCGTGATCCTCTGCCGTAACCTCAACCTTTTCTCTGAGTCGATCATTG‑CCATCGACGGCAGCAAGTTCAAA‑GCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGc                                                                                 >  7:30053/1‑136 (MQ=255)
                                           gTAGCGTCTGCCGTCAGTTCGTGATCCTCTGCCGTAACCTCAACCTTTTCTCTGAGTCGATCATTG‑CCATCGACGGCAGCAAGTTCAAA‑GCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGc                                                                                 >  3:204473/1‑136 (MQ=255)
                                           gTAGCGTCTGCCGTCAGTTCGTGATCCTCTGCCGTAACCTCAACCTTTTCTCTGAGTCGATCATTG‑CCATCGACGGCAGCAAGTTCAAA‑GCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGc                                                                                 >  8:197299/1‑136 (MQ=255)
                                           gTAGCGTCTGCCGTCAGTTCGTGATCCTCTGCCGTAACCTCAACCTTTTCTCTGAGTCGATCATTG‑CCATCGACGGCAGCAAGTTCAAA‑GCAGTGAAAAATCGCGCCCCTAACTTCCCCCAGGCCAAGGTGAAGGc                                                                                 >  2:67447/1‑136 (MQ=255)
                                            tAGCGTCTGCCGTCAGTTCGTGATCCTCTGCCGTAACCTCAACCTTTTCTCTGAGTCGATCATTG‑CCATCGACGGCAGCAAGTGCAAA‑GCAGTGAACAATCGCGACCGTAACTTCCCCCAGGCCAAGGTGAAGGcc                                                                                >  3:21566/1‑136 (MQ=255)
                                              gggTCGGCCTTCAGTTCGTCCACCTCTGCCGAAACCACAACCTTTTCGCTCTGCCCACAATGG‑CCACCGCCGCCAGCAAGTAAAAC‑GCAGTAAAAAACCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGccc                                                                               <  4:9552/133‑1 (MQ=255)
                                              gCGTCTGACGTCCTTTCGTGATCGAGTGCCGTACCCTCAACCTTTTCTCTGTGCCTATCATTG‑CCATCTACGGCAGAAAGTTCAAA‑GCAGTCAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGccc                                                                               <  6:137429/135‑1 (MQ=255)
                                                  cTGCCGTCAGTTCGTGATCCTCTGCCGTAACCTCAACCTTTTCTCTGAGTCGATCATTG‑CCATCGACGGCAGCAAGTTCAAA‑GCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATg                                                                          >  6:280020/1‑136 (MQ=255)
                                                   tcccGTCAGTTCGTCAGCATGTCCCTAAACCTAAACTTTTTCTCTGTGTCTA‑AATTGCCCAGCCCGGGCATCAATTAAAAA‑GCCGTAAACAACCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATgc                                                                         <  2:171779/134‑1 (MQ=255)
                                                      cGTCAGTTCGTGATCCTCTGCCGTAACCTCAACCTTTTCTCTGAGTCGATCATTT‑AAATCGACGGCAGCAAGTTCAAA‑CCAGTGAACAAACGCGACCGTAACTTCACCCACGCCAAGGTTAAGGCCCGCATgaag                                                                       >  1:102292/1‑135 (MQ=255)
                                                      cGTCAGTTCGTGATCCTCTGCCGTAACCTCAACCTTTTCTCTGAGTCGATCATTG‑CCATCGACGGCAGCACGTTCAAA‑GCAGTGAACAATCGCGCCCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATgccg                                                                       >  8:67110/1‑133 (MQ=255)
                                                      cGTCAGTTCGTGATCCTCTGCCGTAACCTCAACCTTTTCTCTGAGTCGATCATTG‑CCATCGACGGCAGCAAGTTCAAA‑GCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATgcag                                                                       >  5:45092/1‑135 (MQ=255)
                                                      cGTCAGTTCGTGATCCTCTGCCGTAACCTCAACCTTTTCTCTGAGTCGATCATTG‑CCATCGACGGCAGCAAGTTCAAA‑GCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATgcag                                                                       >  5:283348/1‑135 (MQ=255)
                                                      cGTCAGTTCGTGATCCTCTGCCGTAACCTCAACCTTTTCTCTGAGTCGATCATTG‑CCATCGACGGCAGCAAGTTCAAA‑GCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATgcag                                                                       >  4:168280/1‑135 (MQ=255)
                                                      cGTCAGTTCGTGATCCTCTGCCGTAACCTCAACCTTTTCTCTGAGTCGATCATTG‑CCATCGACGGCAGCAAGTTCAAA‑GCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATgcag                                                                       >  4:37130/1‑135 (MQ=255)
                                                      cGTCAGTTCGTGATCCTCTGCCGTAACCTCAACCTTTTCTCTGAGTCGATCATTG‑CCATAGACGGCAGCAAGTTCAAA‑GCAGTGAACAATCGCGACCGTAACTTAACCCAGGCCAAGGTGAAGGCCCGCATgcag                                                                       >  8:125555/1‑135 (MQ=255)
                                                        tCAGTTCGTGATCCTCTGCCGTAACCTCAACCTTTTCTCTGAGTCGATCATTG‑CCATCGACGGCAGCAAGTTCAAA‑GCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATgcagca                                                                     >  5:271319/1‑135 (MQ=255)
                                                           gTTCGTGATCCTCTGCCGTAACCTCAACCTTTTCTCTGAGTCGATCATTG‑CCATCGACGGCAGCAAGTTCAAA‑GCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATc                                                                 >  4:13468/1‑136 (MQ=255)
                                                            tgcGTGAGCCTCTGCCGTAACCTCAACCTTTTCTCTGAGTCGATCATTG‑CCATCGTCGGCAGCAAGTTCAAC‑GCAGTCAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCg                                                                <  2:191056/134‑1 (MQ=255)
                                                              ggTGAGCCTCTGCCGAACCCTAAACTTTTTTTCTGTGTGTATAATTC‑CCATCGAGGGCAGAAATTAAAAC‑GAAGTCAAAAACCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCga                                                               <  5:88086/134‑1 (MQ=255)
                                                              cGTGATCCTCTGCCGTAACCTAAACCTTTTCTCTGAGTCGATCATTG‑CCATCGACGGCAGCAAGTTCAAC‑CCAGTCAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCga                                                               <  7:26899/135‑1 (MQ=255)
                                                               gTGATCCTCTGCCGTAACCTCAACATTTTCTCTGAGTCGATCATTT‑CCATCGACGGCAGCAAGTTCAAA‑TCAGTGAACAATCGCGACCGTAACTTCCCCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCgag                                                              >  1:66584/1‑135 (MQ=255)
                                                                   tCCGCTGCCTTAACATCAAATTTTTCTGTGAGTGGATCATTC‑CCATCTACGGCAGAATTTACAAC‑CCTTTTAAAAACCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCgagcaga                                                          <  4:142153/135‑1 (MQ=255)
                                                                     ctctGCCTTACACACACCCTTTTCTCTGAGTCTATCATTG‑CCATCGCGGGCAGCAAGATAACA‑CACGCCAACAACCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCgagcagagca                                                       <  6:25316/136‑1 (MQ=255)
                                                                     ctctGCCTTAACCTCAACCTTTTCTCTTAGTCGTTCTTTC‑CCATCTCCGGCTGCATGTTCAAT‑CCAGTCAACATTCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCgagcagagca                                                       <  7:157472/136‑1 (MQ=255)
                                                                      tctGCCGTAACCTCATCATTTTCTGTGTGTCTATCATTG‑CCAGCGAGGGCAGCAATTTCAAC‑CCAGTCAACAAACGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCAt                                                      <  1:79264/136‑1 (MQ=255)
                                                                       ctGCCTTAACCACAACTTTTAGTCTGAGACAATAATTC‑CCATCGACGGCAGAATTTTCAAA‑CCAGTAAACAACCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCAtt                                                     <  1:152537/136‑1 (MQ=255)
                                                                       ctGCCGTAACCTCAACCTTTTCTCTGAGTCGATAATTG‑CCATCGAGGGCAGAAAGTTCAAA‑GCAGTGAAAAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCAtt                                                     <  2:25/136‑1 (MQ=255)
                                                                         gcctaaaCCACAACGTTTTCTCTGAGTCCATCATTG‑CCATCGAGGGCAGAAAGTCAAAC‑CCAGTCAACAACCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGa                                                   <  4:34387/131‑1 (MQ=255)
                                                                         gCCGTAACCTCAACCTTTTCTCTGAGTCGATCATTG‑CCATCGACGGCAGCAAGTTCAAA‑GCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCAtt                                                     >  8:132514/1‑134 (MQ=255)
                                                                         gCCGTAACCTCAACATTTTCTCTCAGTGTATCATGG‑ACATCTAGGGCAGCATTTTCAAA‑GCATTAAACATTCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGa                                                   <  4:251510/136‑1 (MQ=255)
                                                                         gCCGCACCCTCACCCTTTGCTCTGAGGCTATCATTG‑CCATCGACGCCAGCAAGTTCAAA‑GACGTCAACAACCGCGACCGTAACTTCACCCAGGCCGAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGa                                                   <  6:43775/136‑1 (MQ=255)
                                                                         gCCGAAACCTCAACATTTTCTCTGAGTCTATCATTG‑CCATCGACGGCAGCAAGTTCAAA‑GCTTTCAAAAAACGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGa                                                   <  7:279084/136‑1 (MQ=255)
                                                                         cccttAACCTCAACCTTTTCTCTTAGTCGATCATGC‑CCATCCACGGCAGCAA‑TTCAAACCCAGTCAAAAACCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGa                                                   <  3:66802/132‑1 (MQ=255)
                                                                          ccttacccTCAACCTTTTCTCTGATGCTATCATTG‑CCATCGCGGGCAGCACGTTCAAA‑CCCGTCAACAAACGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAg                                                  <  2:29650/130‑1 (MQ=255)
                                                                          ccttAACCTCAACCTTTTCTCTGAGTCGATCATTC‑CAATCGAGGGCAGCAAGTTAAAA‑GCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGa                                                   <  3:31127/132‑1 (MQ=255)
                                                                          ccttAACATCAACCTTTTCTCTGAGTCTATCATTC‑CAATCGACGGCACAAAGTTCAAA‑GAATTCAAAAAACGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGa                                                   <  3:233041/132‑1 (MQ=255)
                                                                          ccgaacccTCAACCTTTCATCGGAGTGCTTAATTC‑CCATCTACGGCAGAATTTTAAAC‑GCAGTAAACAACCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGa                                                   <  4:254011/129‑1 (MQ=255)
                                                                          ccGTAACCTCAACCTTTTCTCTGAGTCGATCATTG‑CCATCGACGGCAGCAAGTTCAAA‑GCAGTGAAAAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGa                                                   <  7:212087/135‑1 (MQ=255)
                                                                          ccGTAACCTCAACCTTTTCTCTGAGTCCATCATTG‑CCATCGACGGCAGCAAGTTCAAA‑GCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAg                                                  <  6:209726/136‑1 (MQ=255)
                                                                          ccGTAACCTAAACCTTTTCTCTGAGCCTATAATTG‑CCATCGACGGCAGAAATTTAAAA‑GCAGTAAAAAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGa                                                   <  5:197819/135‑1 (MQ=255)
                                                                          ccGAAACCTCAACTTTTTCTCTGCGTCTATATTTG‑CCAGCGAGGGCAGCATTTTCAAA‑CCAGTCAACAACCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGa                                                   <  5:233043/135‑1 (MQ=255)
                                                                          ccGAAACCTCAACCTTTTCTCTGCGGCTATCATGG‑CAATCGACGGAAGCAAGTTCAAC‑GCAGTCAACAACCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGa                                                   <  2:185588/135‑1 (MQ=255)
                                                                          ccGAAACCTAAACCTTTTTTCTTTGTCGATCTTTC‑CCATGCCCGGCAGCAAGTTCAAA‑CCTTTCAACAACCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGa                                                   <  4:105626/135‑1 (MQ=255)
                                                                           cGTAACCTCAACCTTTTCTCTGAGTCGATCATTG‑CCATCGACGGCAGCAAGTTCAAA‑GCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAgc                                                 >  8:215153/1‑136 (MQ=255)
                                                                                ccTCAACCTTTTCTCTGAGTCGATCATTG‑CCATCGACGGCAGCAAGTTCAAA‑GCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTAc                                            >  4:232624/1‑136 (MQ=255)
                                                                                ccTCAACCTTTTCTCTGAGTCGATCATTG‑CCATCGACGGCAGCAAGTTCAAA‑GCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGCTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTAc                                            >  6:182006/1‑136 (MQ=255)
                                                                                 cTCAACCTTTTCTCTGAGTCGATAATTG‑CCATCGACGGCAGCAAGTTCAAA‑GCAGTCAAAAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTAcc                                           <  2:15689/136‑1 (MQ=255)
                                                                                 cTCAACATTTTCTCTTAGTCGATCTTTG‑CCAGCGACGGCAGCAAGTTCAAA‑GCTGTCAACAACCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTAcc                                           <  3:227447/136‑1 (MQ=255)
                                                                                 cTCAACATTTTCTCTGATTCGATCTTGC‑CCATCGACGGCAGAAAGTTCAAC‑GCTGTAAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTAcc                                           <  8:264358/136‑1 (MQ=255)
                                                                                  tCAACCTTTTCTCTGAGTCGATCATTG‑CCATCGACGGCAGCAAGTTCAAA‑GCAGTGAACAATCCCCACCGAAACTTCACCCCGGCCAAGGAGAAGGCCCGCATGCAGCAAAACGAGCAGAGCATTGAGCGCTACCt                                          >  6:63848/1‑136 (MQ=255)
                                                                                      ctttttATCTGTGCCTATCATTC‑CCATCTCCGGCACCAAGTCAAAC‑GCTCTAAAAAACCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACa                                      <  3:160107/134‑1 (MQ=255)
                                                                                       cTTTTCTCTGAGTCGATCATTG‑CCATCGACGGCAGCAAGTTCAAA‑GCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGa                                                   >  8:196106/1‑122 (MQ=255)
                                                                                       cTTTTCTCTGAGTCGATCATTG‑CCATCGACGGCAGCAAGTTCAAA‑GCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGa                                                   <  7:196106/122‑1 (MQ=255)
                                                                                       cTTTTCTCTGAGTCGATCATTG‑CCATCGACGGCAGCAAGTTCAAA‑GCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTCCCTTACAg                                     >  2:103265/1‑136 (MQ=255)
                                                                                       cTTTTCTCTGAGTCGATCATTG‑CCATCGACGGCAGCAAGTTCAAA‑GCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAg                                     >  3:118843/1‑136 (MQ=255)
                                                                                       cTTTTCTCTGAGTCGATCATTG‑CCATCGACGGCAGCAAGTTCAAA‑GCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAg                                     >  5:188644/1‑136 (MQ=255)
                                                                                       cTTTTCTCTGAGTCGATCATTG‑CCATCGACGGCAGCAAGTTCAAA‑GCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAg                                     >  1:191114/1‑136 (MQ=255)
                                                                                              ctGAGTCGATCATTG‑CCATCGACGGCAGCAAGTTCAAA‑GCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAATGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCTATGGa                              >  2:260847/1‑136 (MQ=255)
                                                                                              ctGAGTCGATCATTG‑CCATCGACGGCAGCAAGTTCAAA‑GCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGa                              >  6:187775/1‑136 (MQ=255)
                                                                                                    cGATCATTG‑CCATCGACGGCAGCAAGTTCAAA‑GCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGATTCgg                         >  5:258647/1‑135 (MQ=255)
                                                                                                    cGATCATTG‑CCATCGACGGCAGCAAGTTCAAA‑GCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGATTCgg                         >  1:243996/1‑135 (MQ=255)
                                                                                                     gATCATTG‑CCATCGAGGGCAGCAAGTTCAAA‑GCAGTCAAAAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGATTCGGc                        <  7:29509/135‑1 (MQ=255)
                                                                                                       tctttG‑CCATCGACGGCAGCAAGTTCAAA‑GCAGTCAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGATTCGGc                        <  6:49490/130‑1 (MQ=255)
                                                                                                         aTTG‑CCATCGACGGCAGCAAGTTCAAA‑GCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGATTCGGCAGAcc                   >  1:175759/1‑136 (MQ=255)
                                                                                                         aTTG‑CCATCGACGGCAGCAAGTTCAAA‑GCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGATTCGGCAGAcc                   >  5:96051/1‑136 (MQ=255)
                                                                                                         aTTG‑CCATCGACGGCAGCAAGTTCAAA‑GCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGATTCGGCAGAcc                   >  8:177987/1‑136 (MQ=255)
                                                                                                          atG‑CCATCGACGGCAGCAAGTTCAAA‑GCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGATTCGGCAGACCg                  >  1:102720/2‑136 (MQ=255)
                                                                                                          atG‑CCAGCCACGTCAGCACGTACAAA‑CCAGTCACCAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGATTCGGCAGACCg                  <  4:28992/135‑1 (MQ=255)
                                                                                                           ttcccATCGCCGGCATCAACTTCAAA‑GAAGTTAACACCCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTAACAGCGATGGATTCGGCAGACCg                  <  3:298166/133‑1 (MQ=255)
                                                                                                           ttcccATCCACGCCAGCAAGTTCAAC‑GCCGTCAAAAACCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGATTCGGCAGACCg                  <  1:22823/133‑1 (MQ=255)
                                                                                                               cATCGACGGCAGCAAGTTCAAA‑GCAGTGAACAATCGCGACCGTAACTTAACCCAGGCCAAGGTTAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGATTCGGCAGACCGGGc               >  7:284219/1‑135 (MQ=255)
                                                                                                                atcgcgggCAGCAAGTTCAAC‑CCATTCAAAAACCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGATTCGGCAGACCGGGCaa             <  6:271319/130‑1 (MQ=255)
                                                                                                                     cGGCCGCAAGTTCAAA‑GCCGGCACCAAACGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGATTCGGCAGACCGGGCAATGCCg        <  6:282085/136‑1 (MQ=255)
                                                                                                                     cGGCATCAAGTTCCAC‑TCTGTCAAAAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGATTCGGCAGACCGGGCAATGCCg        <  5:199630/136‑1 (MQ=255)
                                                                                                                     cGGCAGCAAGTTCAAC‑GCAGTCAAAAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGATTCGGCAGACCGGGCAATGCCg        <  6:113722/136‑1 (MQ=255)
                                                                                                                     cGGCAGAAAGTTCAAC‑GCAGTCAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGATTCGGCAGACCGGGCAATGCCg        <  7:79980/136‑1 (MQ=255)
                                                                                                                         agcaAGTTCAAA‑GCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGATTCGGCAGACCGGGCAATGCCGGAgg    >  5:121035/1‑136 (MQ=255)
                                                                                                                         agcaAGTTCAAA‑GCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGATTCGGCAGACCGGGCAATGCCGGAgg    >  4:4662/1‑136 (MQ=255)
                                                                                                                         agcaAGTTCAAA‑GCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGATTCGGCAGACCGGGCAATGCCGGAgg    >  3:80225/1‑136 (MQ=255)
                                                                                                                           aaaGTTCAAA‑GCAGATAACAACCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGATTCGGCAGACCGGGCAATGCCGGAGGTg  <  2:259126/135‑1 (MQ=255)
                                                                                                                                      |                                                                                                                             
AAAACCATCGCCGACTTTCGCAAGGACAACGGTAAAGCCATTCGTAGCGTCTGCCGTCAGTTCGTGATCCTCTGCCGTAACCTCAACCTTTTCTCTGAGTCGATCATTG‑CCATCGACGGCAGCAAGTTCAAA‑GCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGATTCGGCAGACCGGGCAATGCCGGAGGTG  >  ACB143/2171134‑2171391

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑