Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB143 4,672,065 C→T 8.9% E94K (GAG→AAG)  CJ019_04415 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1434,672,0650CT 8.9% 140.3 / 2.9 45E94K (GAG→AAG) CJ019_04415hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (23/18);  new base T (2/2);  total (25/20)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.66e-01

CTCCAGGGGTTGATCCCCACGATCAGCAGGTGCCCGCCCGGGCGTACGGCACTGGCGGCCTCGCGCAGCAGGCCATGGGGCGACAGGCAGAAGTCCAGGCCATGCTGCAGCACCACCACGTCGGCAGCGTGCTCGCTCAACGGCCAGGCTTGTTCTTCGCAGACAATCTCCACACCCGGCAAGGGCGCACCAAGGCGCACATTACGCTGCACCTGGGGGGCTCTGGGCGGTGGCTCGGCGCAGGGCCCGTAATGCACGAGATAGCC  >  ACB143/4671932‑4672197
                                                                                                                                     |                                                                                                                                    
ctCCAGGGGTTGATCCCCACGATCAGCAGGTGCCCGCCCGGGCGTACGGCACTGGCGGCCTCGCGCAGCAGGCCATGGGGCGACAGGCAGAAGTCCAGGCCATGCTGCAGCACCACCACGTCGGCAGCGTgctcg                                                                                                                                     <  7:271208/135‑1 (MQ=255)
ctCCAGGGGTTGATCCCCACGATCAGCAGGTGCCCGCCCGGGCGTACGGCACTGGCGGCCTCGCGCAGCAGGCCATGGGGCGACAGGCAGAAGTCCAGGCCATGCTGCAGCACCACCACGTCGGCAGCGCgctcgc                                                                                                                                    <  5:247194/136‑1 (MQ=255)
   cAGGGGTTGATCCCCACGATCAGCAGGTGCCCGCCCGGGCGTACGGCACTGGCGGCCTCGCGCAGCAGGCCATGGGGCGACAGGCAGAAGTCCAGGCCATGCTGCAGCACCACCACGTCGGCAGCGTgctcgctc                                                                                                                                  >  5:257089/1‑135 (MQ=255)
   cAGGGGTTGATCCCCACGATCAGCAGGTGCCCGCCCGGGCGTACGGCACTGGCGGCCTCGCGCAGCAGGCCATGGGGCGACAGGCAGAAGTCCAGGCCATGCTGCAGCACCACCACGTCGGCAGCGTgctcgctc                                                                                                                                  >  3:37815/1‑135 (MQ=255)
   cAGGGGTTGATCCCCACGATCAGCAGGTGCCCGCCCGGGCGTACGGCACTGGCGGCCTCGCGCAGCAGGCCATGGGGCGACAGGCAGAAGTCCAGGCCATGCTGCAGCACCACCACGTCGGCAGCGTgctcgctc                                                                                                                                  >  2:208417/1‑135 (MQ=255)
   cAGGGGTTGATCCCCACGATCAGCAGGTGCCCGCCCGGGCGTACGGCACTGGCGGCCTCGCGCAGCAGGCCAGCGGGCGACAGGCAGAAGTCCAGGCCATGCTGCAGCACCACCACGTCGGCCGCGTGCTCGCTCa                                                                                                                                 >  1:10342/1‑136 (MQ=255)
     ggggTTGATCCCCACGATCAGCAGGTGCCCGCCCGGGCGTACGGCACTGGCGGCCTCGCGCAGCAGGCCATGGGGCGACAGGCAGAAGTCCAGGCCATGCTGCAGCACCACCACGTCGGCAGCGTGCTCGCTCAAc                                                                                                                               <  5:104008/136‑1 (MQ=255)
     ggggTTGATCCCCACGATCAGCAGGTGCCCGCCCGGGCGTACGGCACTGGCGGCCTCGCGCAGCAGGCCATGGGGCGACAGGCAGAAGTCCAGGCCATGCTGCAGCACCACCACGTCGGCAGCGTGCTCGCTCAAc                                                                                                                               >  7:42943/1‑136 (MQ=255)
     ggggTTGATCCCCACGATCAGCAGGTGCCCGCCCGGGCGTACGGCACTGGCGGCCTCGCGCAGCAGGCCATGGGGCGACAGGCAGAAGTCCAGGCCATGCTGCAGCACCACCACGTCGGCAGCGTGCTCGCTCAAc                                                                                                                               <  7:195709/136‑1 (MQ=255)
     ggggGTGATCCCCACGATCAGCAGGTGCCCGCCCGGGCGTACGGCACTGGCGGCCTCGCGCAGCAGGCCATGGGGCGACAGGCAGAAGTCCAGGCCATGCTGCAGCACCACCACTTCGGCAGCGTGCTCGCTCAAc                                                                                                                               <  1:296552/136‑1 (MQ=255)
       ggTTGATCCCCACGATCAGCAGGTGCCCGCCCGGGCGTACGGCACTGGCGGCCTCGCGCAGCAGGCCATGGGGCGACAGGCAGAAGTCCAGGCCATGCTGCAGCACCACCACGTCGGCAGCGTGCTCGCTCACCgg                                                                                                                             >  1:142424/1‑136 (MQ=255)
        gTTGATCCCCACGATCAGCAGGTGCCCGCCCGGGCGTACGGCACTGGCGGCCTCGCGCAGCAGGCCATGGGGCGACAGGCAGAAGTCCAGGCCATGCTGCAGCACCACCACGTCGGCAGCGTGCTCGCTCAACGGc                                                                                                                            >  7:243171/1‑136 (MQ=255)
        gTTGATCCCCACGATCAGCAGGTGCCCGCCCGGGCGTACGGCACTGGCGGCCTCGCGCAGCAGGCCATGGGGCGACAGGCAGAAGTCCAGGCCATGCTGCAGCACCACCACGTCGGCAGCGTGCTCGCTCAACGGc                                                                                                                            >  7:225169/1‑136 (MQ=255)
              ccccACGATCAGCAGGTGCCCGCCCGGGCGTACGGCACTGGCGGCCTCGCGCAGCAGGCCATGGGGCGACAGGCAGAAGTCCAGGCCATGCTGCAGCACCACCACGTCGGCAGCGTGCTCGCTCAACGGCCAGGCt                                                                                                                      >  8:234796/1‑136 (MQ=255)
              ccccACGATCAGCAGGTGCCCGCCCGGGCGTACGGCACTGGCGGCCTCGCGCAGCAGGCCATGGGGCGACAGGCAGAAGTCCAGGCCATGCTGCAGCACCACCACGTCGGCAGCGTGCTCGCTCAACGGCCAGGCt                                                                                                                      >  8:98056/1‑136 (MQ=255)
               cccACGATCAGCAGGTGCCCGCCCGGGCGTACGGCACTGGCGGCCTCGCGCAGCAGGCCATGTTGCGACAGGCAGAAGGCCAGGCGATGCTGCAGCAACAAGACGTCGGCAGCGTGCTCGCTCAACGGCCAGGCtt                                                                                                                     <  2:209014/136‑1 (MQ=255)
               cccACGATCAGCAGGTGCCCGCCCGGGCGTACGGCACTGGCGGCCTCGCGCAGCAGGCCATGGGGCGACAGGCAGAAGTCCAGGCCATGCTGCAGCACCACCACGTCGGCAGCGTGCTCGCTCAACGGCCAGGCtt                                                                                                                     <  8:243171/136‑1 (MQ=255)
               cccACGATCAGCAGGTGCCCGCCCGGGCGTACGGCACTGGCGGCCTCGCGCAGCAGGCCATGGGGCGACAGGCAGAAGTCCAGGCCATGCTGCAGCACCACCACGTCGGCAGCGTGCTCGCTCAACGGCCAGGCtt                                                                                                                     <  8:64997/136‑1 (MQ=255)
                          cagGTGCCCGCCCGGGCGTACGGCACTGGCGGCCTCGCGCAGCAGGCCATGGGGCGACAGGCAGAAGTCCAGGCCATGCTGCAGCACCACCACGTCGGCAGCGTGCTCGCTCAACGGCCAGGCTTGTTCTTCGCAg                                                                                                          <  7:101811/136‑1 (MQ=255)
                          cagGTGCCCGCCCGGGCGTACGGCACTGGCGGCCTCGCGCAGCAGGCCATGGGGCGACAGGCAGAAGTCCAGGCCATGCTGCAGCACCACCACGTCGGCAGCGTGCTCGCTCAACGGCCAGGCTTGTTCTTCGCAg                                                                                                          <  5:93227/136‑1 (MQ=255)
                                   gcccgGGCGTACGGCACTGGCGGCCTCGCGCAGCAGGCCATGGGGCGACAGGCAGAAGTCCAGGCCATGCTGCAGCACCACCACGTCGGCAGCGTGCTCGCTCAACGGCCAGGCTTGTTCTTCGCAGACAAtctc                                                                                                  >  6:117229/1‑135 (MQ=255)
                                   gcccgGGCGTACGGCACTGGCGGCCTCGCGCAGCAGGCCATGGGGCGACAGGCAGAAGTCCAGGCCATGCTGCAGCACCACCACGTCGGCAGCGTGCTCGCTCAACGGCCAGGCTTGTTCTTCGCAGACAATCTcc                                                                                                 <  5:21952/136‑1 (MQ=255)
                                   gcccgGGCGTACGGCACTGGCGGCCTCGCGCAGCAGGCCATGGGGCGACAGGCAGAAGTCCAGGCCATGCTGCAGCACCACCACGTCGGCAGCGTGCTCGCTCAACGGCCAGGCTTGTTCTTCGCAGACAATCTcc                                                                                                 <  8:24185/136‑1 (MQ=255)
                                   gcccgGGCGTACGGCACTGGCGGCCTCGCGCAGCACGCCATGGGGCGACAGGCAGAAGTCCAGGCCCTGCTCCAGCACCACCACGTCGGCAGCGTGCTCGCTCAACGGCCAGGCTTGTTCTTCGCAGACAATCTcc                                                                                                 >  2:133740/1‑136 (MQ=255)
                                                 cACTGGCGGCCTCGCGCAGCAGGCCATGGGGCGACAGGCAGAAGTCCCGGCCATGCTGCAGCACCACCACGTCGGCAGCGTGCTCGCTCAACGGCCAGGCTTGGTCTTCGCAGACAATCTCCACCCCCGGCACggg                                                                                   >  5:161875/1‑136 (MQ=255)
                                                  aCTGGCGGCCTCGCGCAGCAGGCCATGGGGCGACAGGCAGAAGTCCAGGCCATGCTGCAGCACCACCACGTCGGCAGCGTGCTCGCTCAACGGCCAGGCTTGTTCTTCGCAGACAATCTCCACACCCGGCAAGGgc                                                                                  <  5:152093/136‑1 (MQ=255)
                                                      gcggcCTCGCGCAGCAGGCCATGGGGCGACAGGCAGAAGTCCAGGCCATGCTGCAGCACCACCACGTCGGCAGCGTGCTCGCTCAACGGCCAGGCTTGTTCTTCGCAGACAATCTCCACACCCGGCAAGGgcgccc                                                                              >  1:251599/1‑134 (MQ=255)
                                                      gcggcCTCGCGCAGCAGGCCATGGGGCGACAGGCAGAAGTCCAGGCCATGCTGCAGCACCACCACGTCGGCAGCGTGCTCGCTCAACGGCCAGGCTTGTTCTTCGCAGACAATCTCCACACCCGGCAAGGGCGCa                                                                               <  7:98056/135‑1 (MQ=255)
                                                                   gcagGCCATGGGGCGACAGGCAGAAGTCCAGGCCATGCTGCAGCACCACCACGTCGGCAGCGTGCTCGCTCAACGGCCAGGCTTGTTCTTCGCAGACAATCTCCACACCCGGCAAGGGCGCACCAAGGCGCACAt                                                                  >  3:111231/1‑135 (MQ=255)
                                                                              ggCGACAGGCAGAAGTCCAGGCCATGCTGCAGGACCACCACGTCGGCAGCGTGCTCGCTCAACGGCCAGGCTTGTTCTTCGGAGACAATCTCCACACCCGGCAAGGGCGCACCAAGGCGCACATTACGCTGCACCt                                                      <  3:294987/136‑1 (MQ=255)
                                                                               tcGACATGCAGAAGTCCATGCCATGCTGCAGCACCAACACGTAGGCATCGTTCTCGCTCAACGTCCAGTCTTGATCTTAGAATACAATCTCCACACCCGGCAATGGCGCACCAAGGCGCACATTAATCTGCACCTg                                                     <  5:255951/135‑1 (MQ=255)
                                                                                     ggCAGAAGTCCAGGCCATGCTGCAGCACCACCACGTCGGCAGCGTGCTCGCTCAACGGCCAGGCTTGTTATTCGCAGACAATCTCCACACCCGGCAAGGGCGCACCAAGGCGCACATTACGCTGCACCTGGGGGGc                                               >  7:182521/1‑136 (MQ=255)
                                                                                                aGGCCATGCTGCAGCACCACCACGTCGGCAGCGTGCTCGCGCAACGGCCAGGCTTGTTCTTCGCAGACAATCTCCACACCCGGCAAGGGCGCACCAAGGCGCACATTACGCTGCACCTGGGGGGCTCTGGGCGGTg                                    <  6:109776/136‑1 (MQ=255)
                                                                                                   ccATGCTGCAGCACCACCACGTCGGCAGCGTGCTCGCTCAACGGCCAGGCTTGTTCTTCGCAGACAATCTCCACACCCGGCAAGGGCGCACCAAGGCGCACATTACGCTGCACCTGGGGGGCTCTGGGCGGTGGCt                                 >  3:14896/1‑136 (MQ=255)
                                                                                                      tgctgcAGCACCACCACGTCGGCAGCGTGCTCGCTCAACGGCCAGGCTTGTTCTTCGCAGACAATCTCCACACCCGGCAAGGGCGCACCAAGGCGCACATTACGCTGCACCTGGGGGGCTCTGGGCGGTGGCTCgg                              <  7:234796/136‑1 (MQ=255)
                                                                                                      tgctgcAGCACCACCACGTCGGCAGCGTGCTCGCTCAACGGCCAGGCTTGTTCTTCGCAGACAATCTCCACACCCGGCAAGGGCGCACCAAGGCGCACATTACGCTGCACCTGGGGGGCGCTGGGCGGTGGCTCgg                              <  5:160685/136‑1 (MQ=255)
                                                                                                         tgcAGCACCACCACGTCGGCAGCGTGCTCGCTCAACGGCCAGGCTTGTTCTTCGCAGACAATCTCCACACCCGGCAAGGGCGCACCAAGGCGCACATTACGCTGCACCTGGGGGGCTCTGGGCGGTGGCTCGgcg                            >  4:134721/1‑135 (MQ=255)
                                                                                                              caccaccacGTCGGCAGCGTGCTCGCTCAACGGCCAGGCTTGTTCTTCGCAGACAATCTCCACACCCGGCAAGGGCGCACCAAGGCGCACATTACGCTGCACCTGGGGGGCTCTGGGCGGTGGCTCGGCGCAGGGc                      >  7:262052/1‑136 (MQ=255)
                                                                                                                   ccacGTCGGCAGCGTGCTCGCTCAACGGCCAGGCTTGTTCTTCGCAGACAATCTCCACACCCGGCAAGGGCGCACCAAGGCGCACATTACGCTGCACCTGGGGGGCTCTGGGCGGTGGCTCGGCGCAGGGCCCGt                  >  2:155276/1‑135 (MQ=255)
                                                                                                                   ccacGTCGGCAGCGTGCTCGCTCAACGGCCAGGCTTGTTCTTCGCAGACAATCTCCACACCCGGCAAGGGCGCACCAAGGCGCACATTACGCTGCACCTGGGGGGCTCTGGGCGGTGGCTCGGCGCAGGGCCCGt                  >  8:188005/1‑135 (MQ=255)
                                                                                                                   ccacGTCGGCAGCGTGCTCGCTCAACGGCCAGGCTTGTTCTTCGCAGACAATCTCCACACCCGGCAAGGGCGCACCAAGGCGCACATTACGCTGCACCTGGGGGGCTCTGGGCGGTGGCTCGGCGCAGGGCCCGt                  >  1:4409/1‑135 (MQ=255)
                                                                                                                      cGTCGGCAGCGTGCTTGCTCAACGGCCAGGCTTGTTCTTCGCAGACAATCTCCACACCCGGCAAGGGCGCACCAAGGCGCACATTACGCTGCACCTGGGGGGCTCTGGGCGGTgg                                   <  3:70890/115‑1 (MQ=255)
                                                                                                                      cGTCGGCAGCGTGCTTGCTCAACGGCCAGGCTTGTTCTTCGCAGACAATCTCCACACCCGGCAAGGGCGCACCAAGGCGCACATTACGCTGCACCTGGGGGGCTCTGGGCGGTgg                                   <  3:66750/115‑1 (MQ=255)
                                                                                                                      cGTCGGCAGCGTGCTTGCTCAACGGCCAGGCTTGTTCTTCGCAGACAATCTCCACACCCGGCAAGGGCGCACCAAGGCGCACATTACGCTGCACCTGGGGGGCTCTGGGCGGTgg                                   >  4:66750/1‑115 (MQ=255)
                                                                                                                      cGTCGGCAGCGTGCTTGCTCAACGGCCAGGCTTGTTCTTCGCAGACAATCTCCACACCCGGCAAGGGCGCACCAAGGCGCACATTACGCTGCACCTGGGGGGCTCTGGGCGGTgg                                   >  4:70890/1‑115 (MQ=255)
                                                                                                                      cGTCGGCAGCGTGCTCGCTCAACGGCCAGGCTTGTTCTTCGCAGACAATCTCCACACCCGGCAAGGGCGCACCAAGGCGCACATTACGCTGCACCTGGGGGGCTCTGGGCGGTGGCTCGGCGCAGGGCCCGTAATg              >  1:221497/1‑136 (MQ=255)
                                                                                                                      cGTCGGCAGCGTGCTCGCTCAACGGCCAGGCTTGTTCTTCGCAGACAATCTCCACACCCGGCAAGGGCGCACCAAGGCGCACATTACGCTGCACCTGGGGGGCTCTGGGCGGTGGCTCGGCGCAGGGCCCGTAATg              >  6:244136/1‑136 (MQ=255)
                                                                                                                           gCAGCGTGCTCGCTCAACGGCCAGGCTTGTTCTTCGCAGACAATCTCCACACCCGGCAAGGGCGCACCAAGGCGCACATTACGCTGCACCTGGGGGGCTCTGGGCGGTGGCTCGGCGCAGGGCCCGTAATGCAc           >  3:204489/1‑134 (MQ=255)
                                                                                                                             aGCGTGCTCGCTCAACGGCCAGGCTTGTTCTTCGCAGACAATCTcc                                                                                                 <  2:141912/46‑1 (MQ=255)
                                                                                                                             aGCGTGCTCGCTCAACGGCCAGGCTTGTTCTTCGCAGACAATCTcc                                                                                                 >  1:141912/1‑46 (MQ=255)
                                                                                                                                  gctcgctcAACGGCCAGGCTTGTTCTTCGCAGACAATCTCCACACCCGGCAAGGGCGCACCAAGGCGCACATTACGCTGCACCTGGGGGGCTCTGGGCGGTGGCTCGGCGCAGGGCCCGTAATGCACGAGATAgcc  >  6:167259/1‑136 (MQ=255)
                                                                                                                                     |                                                                                                                                    
CTCCAGGGGTTGATCCCCACGATCAGCAGGTGCCCGCCCGGGCGTACGGCACTGGCGGCCTCGCGCAGCAGGCCATGGGGCGACAGGCAGAAGTCCAGGCCATGCTGCAGCACCACCACGTCGGCAGCGTGCTCGCTCAACGGCCAGGCTTGTTCTTCGCAGACAATCTCCACACCCGGCAAGGGCGCACCAAGGCGCACATTACGCTGCACCTGGGGGGCTCTGGGCGGTGGCTCGGCGCAGGGCCCGTAATGCACGAGATAGCC  >  ACB143/4671932‑4672197

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑