Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	ACB143	690,150	T→G	100%	intergenic (+27/‑2432)	bauC → / → PP_RS08750	Putative 3‑oxopropanoate dehydrogenase/tRNA‑Val

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	ACB143	690,150	0	T	G	84.9%	20.2 / ‑4.8	17	intergenic (+27/‑2432)	bauC/PP_RS08750	Putative 3‑oxopropanoate dehydrogenase/tRNA‑Val
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base G (14/0);  minor base C (3/0);  total (17/0)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

GGTGTGCGCTTCTATACCCGTCGCAAGGCCATTACCCAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGC‑GGGGAGGC‑C‑GTCAGGCC‑TC‑CCCGCTTTGTTTTTGGGGGTTGGTTGTTGCTGTTGGGGAGGAACTGTTTTGA  >  ACB143/690028‑690210                                                                                                                              |                                                               ggTGTGCGCTTCTATACCCGTCGCAAGGCCATTACCCAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGC‑GGGGGGGG‑C‑GGGAGGGC‑Ac‑ccccc                                                 >  1:169816/1‑134 (MQ=255)                aCCCGTCGCAAGGCCATTACCCAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGCGGGGGGGGC‑C‑GGCCGCCC‑CC‑CCCCCGTTTTTTTTggggg                                   >  7:126281/1‑136 (MQ=255)                       gCAAGGCCATTACCCAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGCGGGGGGGGC‑G‑GGCGGGCC‑GC‑CCCCCTTTGTTTTTGGGggtgggttt                            >  2:22173/1‑135 (MQ=255)                       gCAAGGCCATTACCCAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGCGGGGGGGGC‑G‑GGCCGGCC‑GC‑CCCCGGTGTTTTTTGGGGgttgtttg                            >  4:199690/1‑136 (MQ=255)                       gCAAGGCCATTACCCAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGCGGGGGGGGC‑G‑GGCCGGCC‑CC‑CCCCGGTTGTTTTTTTGGgtgggttg                            >  5:79683/1‑136 (MQ=255)                       gCAAGGCCATTACCCAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGCGGGGGGGGC‑C‑GGC‑GGCC‑CC‑CCCCCGGTGTTTTTTGGGGGTGggtgt                           >  5:270959/1‑136 (MQ=255)                       gCAAGGCCATTACCCAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGCGGGGGGGGC‑C‑GCCGGGCG‑AC‑CCCCAGTTTTTTTTGGGGG‑TGgttgt                           >  1:85254/1‑136 (MQ=255)                       gCAAGGCCATTACCCAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGCGGGGGGGGC‑C‑GCAAGGCC‑CC‑CCCCCGTGGTTTTTGGgggtgggtgg                            >  2:259458/1‑134 (MQ=255)                       gCAAGGCCATTACCCAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGC‑GGGGGGGG‑C‑GGGAGGCC‑GC‑CCCCCGTTGTTTTTTGGGGGTGgttgt                           >  1:211297/1‑136 (MQ=255)                       gCAAGGCCATTACCCAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGC‑GGGGGGGG‑C‑GGGACGCCGAC‑CCCCGGTTTTTTTTGGGggtgggttt                            >  4:259972/1‑135 (MQ=255)                       gCAAGGCCATTACCCAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGC‑GGGGGGGG‑C‑GGCAGGGC‑GC‑CCCCCGGTGGTTTTTGGGGGGGgttgt                           >  8:278470/1‑136 (MQ=255)                       gCAAGGCCATTACCCAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGC‑GGGGGGGC‑CGGCCACGCC‑GC‑CCCCCGTTTGTTTTGGgggtgggtgt                            >  2:71161/1‑134 (MQ=255)                         aaGGCCATTACCCAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGCGGGGGGGGC‑G‑GGCCGGCC‑CC‑CCCCCTTGGTTTTTGGGGG‑GGgttgttt                         >  5:101476/1‑135 (MQ=255)                         aaGGCCATTACCCAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGC‑GGGGGGGG‑C‑GGCAGGCC‑TC‑CCCGCGTTGTTTTTTGGGGGTGgttgttt                         >  2:72196/1‑135 (MQ=255)                         aaGGCCATTACCCAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGC‑GGGGGGGCGC‑GGCGGGCC‑CC‑CCCCGTGTGTTTTTTTGGGTTGttggtt                          >  7:77523/1‑136 (MQ=255)                                           tGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGC‑GGGGGGGG‑C‑GGCAGGCC‑CCACCCCCGTGTTTTTTGGGGGTGGGTTGTGGGGGTGGGGGGGGaaggt        >  7:153864/1‑133 (MQ=255)                                                gCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGCGGGGGGGGC‑C‑GGCCGCCC‑CC‑CCCCGATGTTTTTTGGGGGTTGTGTG‑TGGGGTTGGGGCGGGACGGGTTTga  >  2:235529/1‑136 (MQ=255)                                                                                                                              |                                                               GGTGTGCGCTTCTATACCCGTCGCAAGGCCATTACCCAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGC‑GGGGAGGC‑C‑GTCAGGCC‑TC‑CCCGCTTTGTTTTTGGGGGTTGGTTGTTGCTGTTGGGGAGGAACTGTTTTGA  >  ACB143/690028‑690210

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑