| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | ACB143 | 690,162 | G→C | 100% | intergenic (+39/‑2420) | bauC → / → PP_RS08750 | Putative 3‑oxopropanoate dehydrogenase/tRNA‑Val |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | ACB143 | 690,162 | 0 | G | C | 95.1% | 16.4 / ‑6.0 | 16 | intergenic (+39/‑2420) | bauC/PP_RS08750 | Putative 3‑oxopropanoate dehydrogenase/tRNA‑Val |
| Reads supporting (aligned to +/- strand): ref base G (1/0); new base C (15/0); total (16/0) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
| Rejected as polymorphism: Polymorphic base substitution creates a homopolymer stretch. | |||||||||||
ACCCGTCGCAAGGCCATTACCCAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGC‑GGGGAGGC‑C‑GTCAGGCC‑TC‑CCCGCTTTGTTTTTGGGGGTTGGTTGTTGCTGTTGGGGAGGAACTGTTTTGA > ACB143/690043‑690210 | aCCCGTCGCAAGGCCATTACCCAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGCGGGGGGGGC‑C‑GGCCGCCC‑CC‑CCCCCGTTTTTTTTggggg > 7:126281/1‑136 (MQ=255) gCAAGGCCATTACCCAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGCGGGGGGGGC‑G‑GGCGGGCC‑GC‑CCCCCTTTGTTTTTGGGggtgggttt > 2:22173/1‑135 (MQ=255) gCAAGGCCATTACCCAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGCGGGGGGGGC‑G‑GGCCGGCC‑GC‑CCCCGGTGTTTTTTGGGGgttgtttg > 4:199690/1‑136 (MQ=255) gCAAGGCCATTACCCAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGCGGGGGGGGC‑G‑GGCCGGCC‑CC‑CCCCGGTTGTTTTTTTGGgtgggttg > 5:79683/1‑136 (MQ=255) gCAAGGCCATTACCCAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGCGGGGGGGGC‑C‑GGC‑GGCC‑CC‑CCCCCGGTGTTTTTTGGGGGTGggtgt > 5:270959/1‑136 (MQ=255) gCAAGGCCATTACCCAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGCGGGGGGGGC‑C‑GCCGGGCG‑AC‑CCCCAGTTTTTTTTGGGGG‑TGgttgt > 1:85254/1‑136 (MQ=255) gCAAGGCCATTACCCAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGCGGGGGGGGC‑C‑GCAAGGCC‑CC‑CCCCCGTGGTTTTTGGgggtgggtgg > 2:259458/1‑134 (MQ=255) gCAAGGCCATTACCCAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGC‑GGGGGGGG‑C‑GGGAGGCC‑GC‑CCCCCGTTGTTTTTTGGGGGTGgttgt > 1:211297/1‑136 (MQ=255) gCAAGGCCATTACCCAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGC‑GGGGGGGG‑C‑GGGACGCCGAC‑CCCCGGTTTTTTTTGGGggtgggttt > 4:259972/1‑135 (MQ=255) gCAAGGCCATTACCCAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGC‑GGGGGGGG‑C‑GGCAGGGC‑GC‑CCCCCGGTGGTTTTTGGGGGGGgttgt > 8:278470/1‑136 (MQ=255) gCAAGGCCATTACCCAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGC‑GGGGGGGC‑CGGCCACGCC‑GC‑CCCCCGTTTGTTTTGGgggtgggtgt > 2:71161/1‑134 (MQ=255) aaGGCCATTACCCAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGCGGGGGGGGC‑G‑GGCCGGCC‑CC‑CCCCCTTGGTTTTTGGGGG‑GGgttgttt > 5:101476/1‑135 (MQ=255) aaGGCCATTACCCAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGC‑GGGGGGGG‑C‑GGCAGGCC‑TC‑CCCGCGTTGTTTTTTGGGGGTGgttgttt > 2:72196/1‑135 (MQ=255) aaGGCCATTACCCAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGC‑GGGGGGGCGC‑GGCGGGCC‑CC‑CCCCGTGTGTTTTTTTGGGTTGttggtt > 7:77523/1‑136 (MQ=255) tGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGC‑GGGGGGGG‑C‑GGCAGGCC‑CCACCCCCGTGTTTTTTGGGGGTGGGTTGTGGGGGTGGGGGGGGaaggt > 7:153864/1‑133 (MQ=255) gCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGCGGGGGGGGC‑C‑GGCCGCCC‑CC‑CCCCGATGTTTTTTGGGGGTTGTGTG‑TGGGGTTGGGGCGGGACGGGTTTga > 2:235529/1‑136 (MQ=255) | ACCCGTCGCAAGGCCATTACCCAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGC‑GGGGAGGC‑C‑GTCAGGCC‑TC‑CCCGCTTTGTTTTTGGGGGTTGGTTGTTGCTGTTGGGGAGGAACTGTTTTGA > ACB143/690043‑690210 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |