| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | ACB143 | 1,835,796 | 1 bp→TT | 100% | coding (2054/2754 nt) | barA ← | Signal transduction histidine‑protein kinaseBarA |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | ACB143 | 1,835,795 | 1 | . | T | 78.9% | 41.9 / 9.9 | 19 | coding (2055/2754 nt) | barA | Signal transduction histidine‑protein kinaseBarA |
| Reads supporting (aligned to +/- strand): ref base . (0/4); new base T (10/5); total (10/9) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.25e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.02e-01 | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
| * | ACB143 | 1,835,796 | 0 | G | T | 94.7% | 48.9 / ‑3.0 | 19 | T685N (ACC→AAC) | barA | Signal transduction histidine‑protein kinaseBarA |
| Reads supporting (aligned to +/- strand): ref base G (0/1); new base T (10/8); total (10/9) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.74e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.88e-01 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
AGGCGCGGCCGTCCATGCCGGGCATCTGCACATCCATCAGCACCAGGTCGAACAATTCTTCCTGCACGGCCTGTACCGCCGCATAACCATTGTTGACGGCCAGTACCTCGGCACCCAGGTCTTCGAGCAGG‑GT‑TTTTACCAGCAGCAGGTTGGCGGCATTGTCGTCGACGCAGAGTATTTTCGGTCGTCGCTGGCCGTTGGGGTTGCTCCCCTCGGCCAGCGGAC > ACB143/1835665‑1835889 || aGGCGCGGCCGTCCATGCCGGGCATCTGCACATCCATCAGCACCAGGTCGAACAATTCTTCCTGCACGGCCTGTACCGCCGCATAACCATTGTTGACGGCCAGTACCTCGGCACCCAGGTCTTCGAGCAGG‑Tt‑ttt < 1:24335/136‑1 (MQ=255) cgGCCGTCCATGCCGGGCATCTGCACATCCATCAGCACCAGGTCGAACAATTCTTCCTGCACGGCCTGTACCGCCGCATAACCATTGTTGACGGCCAGTACCTCGGCACCCAGGTCTTCGAGCAGG‑tt‑tttttac > 1:233525/1‑132 (MQ=255) cgGCCGTCCATGCCGGGCATCTGCACATCCATCAGCACCAGGTCGAACAATTCTTCCTGCACGGCCTGTACCGCCGCATAACCATTGTTGACGGCCAGTACCTCGGCACCCAGGTCTTCGAGCAGG‑tt‑tttttac > 3:89154/1‑132 (MQ=255) gCCGTCCATGCCGGGCATCTGCACATCCATCAGCACCAGGTCGAACAATACTTCCTGCACGGCCTGTACCGCCGCATAACCATTGTTGACGGCCAGTACCTCGGCACCCAGGTCTTCGAGCAGGTTT‑TTTTACcag < 4:22727/136‑1 (MQ=255) aTGCCGGGCATCTGCACATCCATCAGCACCAGGTCGAACAATTCTTCCTGCACGGCCTGTACCGCCGCATAACCATTGTTGACGGCCAGTACCTCGGCACCCAGGTCTTCGAGCAGGTTT‑TTTTACCAGCAGCAgg > 5:252881/1‑136 (MQ=255) tGCCGGGCATCTGCACATCCATCAGCACCAGGTCGAACAATTCTTCCTGCACGGCCTGTACCGCCGCATAACCATTGTTGACGGCCAGTACCTCGGCACCCAGGTCTTCGAGCAGGTTT‑TTTTACCAGCAGCAGGt > 5:240082/1‑136 (MQ=255) gggCATCTGCACATCCATCAGCACCAGGTCGAACAATTCTTCCTGCACGGCCTGTACCGCCGCATAACCATTGTTGACGGCCAGTACCTCGGCACCCAGGTCTTCGAGCAGGTTT‑TTTTACCAGCAGCAGGTTggc > 1:39371/1‑136 (MQ=255) cacaTCCATCAGCACCAGGTCGAACAATTCTTCCTGCACGGCCTGTACCGCCGCATAACCATTGTTGACGGCCAGTACCTCGGCACCCAGGTCTTCGAGCAGG‑GT‑TTTTACCAGCAGCAGGTTGGCGGCATTgt < 4:131065/134‑1 (MQ=255) tccatcAGCACCAGGTCGATCAATTCTTCCTGCACGGCCTGTACCGCCGCATAACCATTGTTGACGGCCAGTACCTCGGCACCCAGGTCTTCGAGCAGG‑TTCTTTTACCAGCAGCAGGTTGGCGGCATTGtcgtcg < 1:64530/136‑1 (MQ=255) gCACCAGGTCGAACAATTCTTCCTGCACGGCCTGTACCGCCGCATAACCATTGTTGACGGCCAGTACCTCGGCACCCAGGTCTTCGAGCAGGTTT‑TTTTACCAGCAGCAGGTTGGCGGCATTGTCGTCGACGCa > 8:108959/1‑134 (MQ=255) gCACCAGGTCGAACAATTCTTCCTGCACGGCCTGTACCGCCGCATAACCATTGTTGACGGCCAGTACCTCGGCACCCAGGTCTTCGAGCAGGTTT‑TTTTACCAGCAGCAGGTTGGCGGCATTGTCGTCGACGCa > 2:141250/1‑134 (MQ=255) gCACCAGGTCGAACAATTCTTCCTGCACGGCCTGTACCGCCGCATAACCATTGTTGACGGCCAGTACCTCGGCACCCAGGTCTTCGAGCAGGTTT‑TTTTACCAGCAGCAGGTTGGCGGCATTGTCGTCGACGCa > 5:154006/1‑134 (MQ=255) cAGGTCGAACAATTCTTCCTGCACGGCCTGTACCGCCGCATAACCATTGTTGACGGCCAGTACCTCGGCACCCAGGTCTTCGAGCAGGTTT‑TTTTACCAGCAGCAGGTTGGCGGCATTGTCGTCGACGCAGAGTAt < 6:154006/136‑1 (MQ=255) gTCGAACAATTCTTCCTGCACGGCCTGTACCGCCGCATAACCATTGTTGACGGCCAGTACCTCGGCACCCAGGTCTTCGAGCAGGTTT‑TTTTACCAGCAGCAGGTTGGCGGCATTGTCGTCGACGCAGAGTAttt > 1:189005/1‑135 (MQ=255) gTCGAACAATTCTTCCTGCACGGCCTGTACCGCCGCATAACCATTGTTGACGGCCAGTACCTCGGCACCCAGGTCTTCGAGCAGGTTT‑TTTTACCAGCAGCAGGTTGGCGGCATTGTCGTCGACGCAGAGTAttt > 7:82162/1‑135 (MQ=255) cGAACAATTCTTCCTGCACGGCCTGTACCGCCGCATAACCATTGTTGACGGCCAGTACCTCGGCACCCAGGTCTTCGAGCAGGTTT‑TTTTACCAGCAGCAGGTTGGCGGCATTGTCGTCGACGCAGAGTATTTTCg > 1:254815/1‑136 (MQ=255) aaTTCTTCCTGCACGGCCTGTACCGCCGCATAACCATTGTTGACGGCCAGTACCTCGGCCCCCAGGTCTTCGAGCAGGTTT‑TTTTACCAGCAGCAGGTTGGCGGCATTGTCGTCGACGAAGAGTATTTTCGgtcgt > 5:263509/1‑136 (MQ=255) cTGGACGGCCTGTACAGCCGCATTACCATTGTTGACGGCCAGTACCTCGGCACCCAGGTCTTCGAGCAGGTTT‑TTTTACCAGCAGCAGGTTGGCGGCATTGTCGTCGACGCAGAGTATTTTCGGTCGTCGCTGGc < 1:46920/135‑1 (MQ=255) cTGCACGGCCTGTACCGCCGCATAACCATTGTTGACGGCCAGTACCTCGGCACCCAGGTCTTCGAGCAGGTTT‑TTTTACCAGCAGCAGGTTGGCGGCATTGTCGTCGACGCAGAGTATTTTCGGTCGTCGCTGGc < 8:91224/135‑1 (MQ=255) gCCTGTACCGCCGCATAACCATTGTTGACCGCCAGTACCTCGGCACCCAGGTCTTCGAGCAGGTTT‑TTTTACCAGCAGCAGGTTGGCGGCATTGTCGTCGACGCAGAGTATTTTCGGTCGTCGCTGGCCGTTgggg < 1:143294/136‑1 (MQ=255) tgtggccGGCCAGTACCTCGGCACCCAGTGCTGGGAGCTTG‑TT‑TTTTACCACCAGCGGGTTGGCGGCTTGGGCGGCGACGCCTTTTATTTTCGGGCGTCGCGGGCCGTGGGGGTTGCCCCCCGCGGCCAGCGGAc < 2:202637/129‑1 (MQ=255) || AGGCGCGGCCGTCCATGCCGGGCATCTGCACATCCATCAGCACCAGGTCGAACAATTCTTCCTGCACGGCCTGTACCGCCGCATAACCATTGTTGACGGCCAGTACCTCGGCACCCAGGTCTTCGAGCAGG‑GT‑TTTTACCAGCAGCAGGTTGGCGGCATTGTCGTCGACGCAGAGTATTTTCGGTCGTCGCTGGCCGTTGGGGTTGCTCCCCTCGGCCAGCGGAC > ACB143/1835665‑1835889 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |