| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | ACB143 | 1,542,102 | 0 | T | G | 53.8% | 1.2 / 11.8 | 13 | intergenic (+31/‑214) | CJ019_01454/groS | hypothetical protein/10 kDa chaperonin |
| Reads supporting (aligned to +/- strand): ref base T (1/5); new base G (7/0); total (8/5) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.66e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.13e-02 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GCCAGCGTGCATTCCAGGATGACCCCTTCCAGGCGCAGCCGCGTGATGGCGGCCACCGCCCGAACGTGATCGAAGGCGAGTACGAGCGCCGCGACAAGTAAGAAGGACC‑GGGGCCGCGCAA‑GC‑GG‑CCCCGGTTTTTTTTTGGCCTGATGAAAAATTTTCTATCTCGAGCCTTGTAATCGGATGTGGCGGCCTCATGTATGTGGTCACCGCAAGGTTTCTGGTGGCAACACCAGACATTACACAGGTGGTTCGCTCACAGCGGGCC > ACB143/1541971‑1542235 | gcCAGCGTGCATTCCAGGATGACCCCTTCCAGGCGCAGCCGCGTGATGGCGGCCACCGCCCGAACGTGATCGAAGGCGAGTACGAGCGCCGCGACAAGTAAGCAGGACC‑GGGGGCGCGCCA‑GCGGG‑CCCCGGgtt > 4:370952/1‑135 (MQ=255)gcCAGCGTGCATTCCAGGATGACCCCTTCCAGGCGCAGCCGCGTGATGGCGGCCACCGCCCGAACGTGATCGAAGGCGAGTACGAGCGCCGCGACAAGTAAGAAGGACC‑GGGGCCGCGCAA‑GC‑GG‑CCCCGGtttt > 8:320144/1‑135 (MQ=255) ccAGCGTGCATTCCAGGATGACCCCTTCCAGGCGCAGCCGCGTGATGGCGGCCACCGCCCGAACGTGATCGAAGGCGAGTACGAGCGCCGCGACAAGTAAGAAGGACC‑GGGGCCGCGCAA‑GC‑GG‑CCCCGGgtttt > 8:228411/1‑135 (MQ=255) gTGCATTCCAGGATGACTCCTTCCAGGCGCAGCCGCGTGATGGCGGCCACCGCCCGAACGTGATCGAAGGCGAGTACGAGCGCCGCGACAAGTAAGAAGGACC‑GGGGCCGCGCAA‑GC‑GG‑CCCCGGGGTTTTTTTg > 7:265303/1‑135 (MQ=255) gTGCATTCCAGGATGACCCCTTCCAGGCGCAGCCGCGTGATGGCGGCCACCGCCCGAACGTGATCGAAGGCGAGTACGAGCGCCGCGACAAGTAAGAAGGACCGGGGGCCCCCCAC‑CG‑GG‑CCCCGGGTTTTTTTTg > 6:123514/1‑136 (MQ=255) gTGCATTCCAGGATGACCCCTTCCAGGCGCAGCCGCGTGATGGCGGCCACCGCCCGAACGTGATCGAAGGCGAGTACGAGCGCCGCGACAAGTAAGAAGGACC‑GGGGGCGCGCCC‑GCGGG‑CCCCGGttttttttt > 3:204013/1‑135 (MQ=255) tCCAGGATGACCCCTTCCAGGCGCAGCCGCGTGATGGCGGCCACCGCCCGAACGTGATCGAAGGCGAGTACGAGCGCCGCGACAAGTAAGAAGGACC‑GGGGCCGCCCCA‑CCGGG‑CCCCGGGTTATTTTgggaggg > 1:285344/1‑131 (MQ=255) cccTTCCAGGCGCAGCCGCGTGATGGCGGCCACCGCCCGAACGTGATCGAAGGCGAGTACGAGCGCCGCGACAAGTAAGAAGGACC‑GGGGCCCCGCAA‑GC‑GG‑CCCCGGGTTTTTTTTTGGCGGATGAAAAAtttt > 7:264668/1‑135 (MQ=255) cTTCCAGGCGCAGCCGCGTGATGGCGGCCACCGCCCGAACGTGATCGAAGGCGAGTACGAGCGCCGCGACAAGTAAGAAGGACC‑GGGGCCGCGCAA‑GC‑GG‑CCCCGGGTTTTTTTTTGGCGGTTGAAAAAttttat > 8:41911/1‑133 (MQ=255) tCCAGGCGCAGCCGCGTGATGGCGGCCACCGCCCGAACGTGATCGAAGGCGAGTACGAGCGCCGCGACAAGTAAGAAGGACC‑GGGGCCCCGCCA‑GC‑GGCCCCCGGGTTTTTTTTGGCGGGGTGGAAAAttttttt > 7:166473/1‑132 (MQ=255) cAGCCGCGTGATGGCGGCCACCGCCCGAACGTGATCGAAGGCGAGTACGAGCGCCGCGACAAGTAAGAAGGACC‑GGGGCCGCGCAA‑GC‑GG‑CCCCGGTTTTTTTTTGGCCTGATGTAAAATTTTATATCTCGAGcc > 7:218221/1‑135 (MQ=255) gcgccCCCCGCCCGACCGTTCTCCACGGCGTGCCCGCGCGCCGCTAAAATTACAACGCCCG‑GGGGCCGCGAAC‑GG‑GG‑CCCCGGTTTTTTTTTGGCCTGATGAAAAATTTTCTATCTCGAGCCTTGTAATCGGAt < 1:73517/134‑1 (MQ=255) ccGAACGTGATCGAAGGCGAGTACGAGCGCCGCGACAAGTAAGAAGGACC‑GGGGCCGCGCAA‑GC‑GG‑CCCCGGGGTTTTTTTGGCCTGATGCAAAATTTTAGATGGCGGGCCGTGTAATGGGATGTGGGGGccgaa > 8:86647/1‑132 (MQ=255) gccgcGAAAATTAA‑AACGCCC‑GGGGCCCCCCAACGG‑GG‑CCCCGGTTTTTTTTTGGCCTGATGAAAAATTTTCTATCTCGAGCCTTGTAATCGGATGTGGCGGCCTCATGTATGTGGTCACCGCAAGGTTTCtggt < 4:291794/135‑1 (MQ=255) gggCCGCCCCC‑GG‑GG‑CCCCGGTTTTTTTTTGGCCTGATGAAAAATTTTCTATCTCGAGCCTTGTAATCGGATGTGGCGGCCTCATGTATGTGGTCACCGCAAGGTTTCTGGTGGCAACACCAGACATTACACAggt < 5:123514/136‑1 (MQ=255) gcccccgc‑gc‑CCCCGGTTTTTTTTTGGCCTGATGAAAAATTTTCTATCTCGAGCCTTGTAATCGGATGTGGCGGCCTCATGTATGTGGTCACCGCAAGGTTTCTGGTGGCAACACCAGACATTACACAGGTGGTTc < 5:143132/130‑1 (MQ=255) gTTTTTTTTTGGCCTGATGAAAAATTTTCTATCTCGAGCCTTGTAATCGGATGTGGCGGCCTCATGTATGTGGTCACCGCAAGGTTTCTGGTGGCAACACCAGACATTACACAGGTGGTTCGCTCACAGCGGGcc < 2:289606/135‑1 (MQ=255) | GCCAGCGTGCATTCCAGGATGACCCCTTCCAGGCGCAGCCGCGTGATGGCGGCCACCGCCCGAACGTGATCGAAGGCGAGTACGAGCGCCGCGACAAGTAAGAAGGACC‑GGGGCCGCGCAA‑GC‑GG‑CCCCGGTTTTTTTTTGGCCTGATGAAAAATTTTCTATCTCGAGCCTTGTAATCGGATGTGGCGGCCTCATGTATGTGGTCACCGCAAGGTTTCTGGTGGCAACACCAGACATTACACAGGTGGTTCGCTCACAGCGGGCC > ACB143/1541971‑1542235 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |