Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	ACB143	3,785,684	0	C	G	33.3%	90.5 / 17.2	65	G225A (GGC→GCC)	dmdC_4	3‑methylmercaptopropionyl‑CoA dehydrogenase
Reads supporting (aligned to +/- strand):  ref base C (19/23);  new base G (21/0);  total (42/23)
Fisher's exact test for biased strand distribution p-value = 5.10e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

CCAGGCGTAGTGCACAGGTGGCGCTGCCCCGGATGCCCAGCTTATGTTCCAGGCCGTCGCAGTACAGTGTATTGCGCTGGCCATCCTCCAGCCATTTCGGCACCAGCAACAGCGAGAGCCC‑GCGGTTGCCGGGCGGCGCATCCGGCAACCGGGCCAACACCAGGTGGAGTATGTCGCGGGTCAGGTCGTGTTCGCCGCCAGAGATGAACAGCTTGTTGCCACTGACCGCGTAGCCACCGTCGCCACCGTCGCCCAGGG  >  ACB143/3785556‑3785813                                                                                                                                  |                                                                                                                                  ccAGGCGTAGTGCACAGGTGGCGCTGCCCCGGATGCCCAGCTTATGTTCCAGGCCGTCGCAGTACAGTGTATTGCGCAGGCCATCCTCCAGCCATTTCGGCACCAGCCACAGCGAGAGCCC‑GAGCGTGACGggcg                                                                                                                             >  8:283461/1‑135 (MQ=255)  cAGGCGTAGTGCACAGGTGGCGCTGCCCCGGATGCCCAGCTTATGTTCCAGGCCGTCGCAGTACAGTGTATTGCGCTGGCCATCCTCCAGCCATTTCGGCACCAGCAACAGCGAGCGCCCGGCGGGTGCGgggggg                                                                                                                            >  6:47085/1‑136 (MQ=255)  cAGGCGTAGTGCACAGGTGGCGCTGCCCCGGATGCCCAGCTTATGTTCCAGGCCGTCGCAGTACAGTGTATTGAGCTGGCCATCCTCCAGCCATTTCGGCACCAGCAACAGCGAGAGCCC‑GCGGTTGCCGggcgg                                                                                                                            >  4:296425/1‑135 (MQ=255)       gTAGTGCACAGGTGGCGCTGCCCCGGATGCCCAGCTTATGTTCCAGGCCGTCGCAGTACAGTGTATTGCGCTGGCCATCCTCCAGCCATTTCGGCACCAGCAACAGCGAGAGCCC‑GCGGTTGCCGGGCGGCGCAt                                                                                                                       <  1:110460/135‑1 (MQ=255)           tGCACAGGTGGCGCTGCCCCGGATGCCCAGCTTATGTTCCAGGCCGTCGCAGTACAGTGTATTGCGCTGGCCATCCTCCAGCCATTTCGGCACCAGCAACAGCGAGAGCCC‑GCGGTTGCCGGGCGGCGCATCCGGc                                                                                                                  <  6:283058/136‑1 (MQ=255)            gCACAGGTGGCGCTGCCCCGGATGCCCAGCTTATGTTCCAGGCCGTCGCAGTACAGTGTATTGCGCTGGCCATCCTCCAGCCATTTCGGCACCAGCAACAGCGAGAGCCC‑GCGGTTGCCGGGCGGCGCATCCGGc                                                                                                                  <  4:251177/135‑1 (MQ=255)             cacaGGTGGCGCTGCCCCGGATGCCCAGCTTATGTTCCAGGCCGTCGCAGTACAGTGTATTGCGCTGGCCATCCTCCAGCCATTTCGGCACCAGCAACAGCGAGAGCCC‑GCGGTTTACAGGCGGCGCATcacgc                                                                                                                  >  8:382348/1‑130 (MQ=255)             cacaGGTGGCGCTGCCCCGGATGCCCAGCTTATGTTCCAGGCCGTCGCAGTACAGTGTATTGCGCTGGCCATCCTCCAGCCATTTCGGCACCAGCAACAGCGAGAGCCC‑GCGGTTGGCCGGCGGCGCCTCCCGcca                                                                                                                >  5:362229/1‑134 (MQ=255)             cacaGGTGGCGCTGCCCCGGATGCCCAGCTTATGTTCCAGGCCGTCGCAGTACAGTGTATTGCGCTGGCCATCCTCCAGCCATTTCGGCACCAGCAACAGCGAGAGCCC‑GCGGTTGCCGGGCGGGGCATCCGGCa                                                                                                                 >  3:73055/1‑135 (MQ=255)               caGGTGGCGCTGCCCCGGATGCCCAGCTTATGTTCCAGGCCGTCGCAGTACAGTGTATTGCGCTGGCCATCCTCCAGCCATTTCGGCACCAGCAACAGCGAGAGCCC‑GCGGTTTGCGGGCGGCGCCTACGGCAAc                                                                                                               >  7:61779/1‑135 (MQ=255)                aGGTGGCGCCGCCCCGGATGCCCAGCTTATGTTCCAGGCCGTCGCAGTACAGTGTATTGCGCTGGCCATCCTCCAGCCATTTCGGCACCAGCAACAGCGAGAGCCC‑GCGGTTGCCGGGCGGCGCATCCGGCAAcc                                                                                                              <  8:347132/135‑1 (MQ=255)                 ggTGGCGCTGCCCCGGATGCCCAGCTTATGTTCCAGGCCGTCGCAGTACAGTGTATTGCGCTGGCCATCCTCCAGCCATTTCGGCACCAGCAACAGCGAGAGCCC‑GCGGTTGCCGGGCGGCGCATCCGGCAACCg                                                                                                             <  1:313240/135‑1 (MQ=255)                    ggCGCTGCCCCGGATGCCCAGCTTATGTTCCAGGCCGTCGCAGTACAGTGTATTGCGCTGGCCATCCTCCAGCCATTTCGGCACCAGCAACAGCGAGAGCCC‑GCGGGTGGCGGGCGGCGCCGCCGGCCCCCGGGc                                                                                                          >  1:30302/1‑135 (MQ=255)                     gcgcTGCCCCGGATGCCCAGCTTATGTTCCAGGCCGTCGCAGTACAGTGTATTGCGCTGGCCATCCTCCAGCCATTTCGGCACCAGCAACCGCGAGAGCCC‑GCGGGTGGCCGGCGGCGGCTCCGGCCACCCGGcc                                                                                                         >  2:131479/1‑135 (MQ=255)                     gcgcTGCCCCGGATGCCCAGCTTATGTTCCAGGCCGTCGCAGTACAGTGTATTGCGCTGGCCATCCTCCAGCCATTTCGGCACCAGCAACAGCGAGAGCCC‑GCGGTTGGCGGGCGGCGCATCCCGGCACCGGGc                                                                                                          >  4:305326/1‑134 (MQ=255)                      cgcTGCCCCGGATGCCCAGCTTATGTTCCAGGCCGTCGCAGTACAGTGTATTGCGCTGGCCATCCTCCAGCCATTTCGGCACCAGCAACAGCGAGAGCCC‑GCGGTTGCCGGGCGGCGCATCCGGCAACCGGGCCa                                                                                                        <  2:44267/135‑1 (MQ=255)                       gcTGCCCCGGATGCCCAGCTTATGTTCCAGGCCGTCGCAGTACAGTGTATTGCGCTGGCCATCCTCCAGCCATTTCGTCACCAGCAACAGCGAGAGCCC‑GCGGTTGCCGGGCGGCTCATCCGGCAACCTGGCCaa                                                                                                       <  7:276888/135‑1 (MQ=255)                       gcTGCCCCGGATGCCCAGCTTATGTTCCAGGCCGTCGCAGTACAGTGTATTGCGCTGGCCATCCTCCAGCCATTTCGGCACCAGCAACAGCGAGAGCCC‑GCGGTTGCCGGGCGGCGCATCCGGCAACCGGGCCAac                                                                                                      <  6:148733/136‑1 (MQ=255)                        cTGCCCCGGATGCCCAGCTTATGTTCCAGGCCGTCGCAGTACAGTGTATTGCGCTGGCCATCCTCCAGCCATTTCGGCACCAGCAACAGCGAGAGCCC‑GGGGTTGGCGGGCGGGGCCGCCGGCGCCCCGGccccc                                                                                                      >  3:183579/1‑132 (MQ=255)                        cTGCCCCGGATGCCCAGCTTATGTTCCAGGCCGTCGCAGTACAGTGTATTGCGCTGGCCATCCTCCAGCCATTTCGGCACCAGCAACAGCGAGAGCCC‑GCGGTTGCCGGGCGGCGCATCCGGCAACCGGGCCCac                                                                                                      >  7:308221/1‑135 (MQ=255)                        cTGCCCCGGATGCCCAGCTTATGTTCCAGGCCGTCGCAGTACAGTGTATTGCGCTGGCCATCCTCCAGCCATTTCGGCACCAGACACAGCGAGAGCCC‑GCGGGTTCCGGGCGGCGGAACCGCCCGCCGGGcgacc                                                                                                      >  2:384853/1‑131 (MQ=255)                        cTGCCCCGGATGCCCAGCTTATGTTCCAGGCCGTCGCAGTACAGTGTATTGCGCGGGCCATCCTCCAGCCATTTCGGCACCAGCAACAGCGAGAGCCC‑GCGGTTGCCGGGCGGCGCATCCGGCAACCGGGCCAac                                                                                                      <  7:357002/135‑1 (MQ=255)                            cccGGATGCCCAGCTTATGTTCCAGGCCGTCGCAGTACAGTGTATTGCGCTGGCCATCCTCCAGCCATTTCGGCACCAGCAACAGCGAGAGCCC‑GCGGTTGCCGGGCGGCGCATCCGGCAACCGGGCCAacac                                                                                                    <  8:115719/133‑1 (MQ=255)                            cccGGATGCCCAGCTTATGTTCCAGGCCGTCGCAGTACAGTGTATTGCGCTGGCCATCCTCCAGCCATTTCGGCACCAGCAACAGCGAGAGCCC‑GCGGTTGCCGGGCGGCGCATCCGGCAACCGGGCCAACAcc                                                                                                   <  1:195917/134‑1 (MQ=255)                             ccGGATGCCCAGCTTATGTTCCAGGCCGTCGCAGTACAGTGTATTGCGCTGGCCATCCTCCAGCCATTTCGGCACCAGCAACAGCGAGAGCCC‑GCGGTTGCCGGGCGGCGCATCCGGCAACCGGGCCAACACCAg                                                                                                 <  1:309744/135‑1 (MQ=255)                                gATGCCCAGCTTATGTTCCAGGCCGTCGCAGTACAGTGTATTGCGCTGGCCATCCTCCAGCCATTTCGGCACCAGCAACAGCGAGAGCCC‑GCGGTTGCCGGGCGGCGCATCCGGCAACCGGGCCAACACCAGGTg                                                                                              <  2:158042/135‑1 (MQ=255)                                       aGCTTCTGTTCCCGGCCGTCGCAGTACAGTGTCTTGCGCTGGCCATCCTCCAGCCATTTCGGCACCACCAACAGCGAGAGCCC‑GCGGTTGCCGGGCGGCGCATCCGGCAACCGGGCCAACACCAGGTGGAGTATg                                                                                       <  3:144995/135‑1 (MQ=255)                                       aGCTTATGTTCCAGGCCGTCGCAGTACAGTGTATTGCGCTGGCCATCCTCCAGCCATTTCGGCACCAGCAACAGCGAGAGCCC‑GCGGTTGCCGGGCGGCGCATCCGGCAACCGGGCCAACACCAGGTGGAGTATg                                                                                       <  2:339806/135‑1 (MQ=255)                                       aGCTTATGTTCCAGGCCGTCGCAGTACAGTGTATTGCGCTGGCCATCCTCCAGCCATTTCGGCACCAGCAACAGCGAGAGCCC‑GCGGTTGCCGGGCGGCGCATCCGGCAACCGGGCCAACACCAGGTGGAGTATg                                                                                       <  4:363801/135‑1 (MQ=255)                                       aGCTTATGTTCCAGGCCGTCGCAGTACAGTGTATTGCGCTGGCCATCCTCCAGCCATTTCGGCACCAGCAACAGCGAGAGCCC‑GCGGTCGCCGGGCGGCGCATCCGGCAACCGGGCCAACACCAGGTGGAGTATg                                                                                       <  2:90849/135‑1 (MQ=255)                                        gCTTATGTTCCAGGCCGTCGCAGTACAGTGTATTGCGCTGGCCATCCTCCAGCCATTTCGGCACCAGCAACAGCGAGAGCCC‑GCGGTTTGCGGGGGGCGCCTCCCGCAACCCGGCCCACAACACGGGGAGGATg                                                                                       >  6:346721/1‑134 (MQ=255)                                        gCTTATGTTCCAGGCCGTCGCAGTACAGTGTATTGCGCTGGCCATCCTCCAGCCATTTCGGCACCAGCAACAGCGAGAGCCC‑GCGGGTTCCCGGCGGCGCCTCCGGCCACCCGGCCAACACCACGTGGAGTATGt                                                                                      >  1:6074/1‑135 (MQ=255)                                          ttATGTTCCAGGCCGTCGCAGTACAGTGTATTGCGCTGGCCATCCTCCAGCCATTTCGGCACCAGCAACAGCGAGAGCCC‑GCGGTTGCCGGGCGGTGCATCCGGCAACCGGGCCAACACCAGGTGGAGTATGTcg                                                                                    <  4:243357/135‑1 (MQ=255)                                          ttATGTTCCAGGCCGTCGCAGTACAGTGTATTGCGCTGGCCATCCTCCAGCCATTTCGGCACCAGCAACAGCGAGAGCCC‑GCCGTTGCCGGGCGGCGGATCCCGCCACCGGGCCCACCCCACGGGGAGGATGtgg                                                                                    >  2:241090/1‑133 (MQ=255)                                                      ccGTCGCAGTACAGTGTATTGCGCTGGCCATCCTCCAGCCATTTCGGCACCAGCAACAGCGAGAGCCC‑GCGGTTGGCGGGCGGCGCCTCCCCCCACCCGGCCACAACCACGGGGAGTATTGAGCGGGGCACGTCg                                                                        >  3:338500/1‑135 (MQ=255)                                                      ccGTCGCAGTACAGTGTATTGCGCTGGCCATCCTCCAGCCATTTCGGCACCAGCAACAGCGAGAGCCC‑GCGGTTGCCGGGCGGCGCATCCGGCAACCGGGCCAACACCAGGTGGAGTATGTCGCGGGGGAGGTCg                                                                        >  2:72909/1‑135 (MQ=255)                                                            cAGTACAGTGTATTGCGCTGGCCATCCTCCAGCCATTTCGGCACCAGCAACAGCGAGAGCCC‑GCGGTTTGCGGGCGGCGCATACGGCAAACGGGCCAACCCCACGGGGTGTATGTAGCGGGTCAGGTGGTGttag                                                                  >  3:188298/1‑133 (MQ=255)                                                             aGTACAGTGTATTGCGCTGGCCATCCTCCAGCCATTTCGGCACCAGCAACAGCGAGAGCCC‑GCGGTTGCCGGGGGGCGGCGACGGCCACCCGGCCAACACCACGTGGAGTAAGTTGGGGGTCAGGGAGTGTTggc                                                                 >  8:144510/1‑135 (MQ=255)                                                             aGTACAGTGTATTGCGCTGGCCATCCTCCAGCCATTTCGGCACCAGCAACAGCGAGAGCCC‑GCGGGTGCCGGGCGGCGCATCCGGCAACCGGGCCAACACCAGGTGGAGTATGTCGCGGGTCAGGTCGTGTTcgc                                                                 <  2:414702/135‑1 (MQ=255)                                                               tACAGTGTATTGCGCTGGCCATCCTCCAGCCATTTCGGCACCAGCAACAGCGAGAGCCC‑GCGGTTGCCGGGCGGCGCATCCGGCAACCGGGCCAACACCAGGTGGAGTATGTCGCGGGTCAGGTCGTGTTcgccg                                                               <  3:215976/135‑1 (MQ=255)                                                                       aTTGCGCTGGCCATCCTCCAGCCATTTCGGCACCAGCAACAGCGAGAGCCC‑GCGGTTTGCGGGGGGCGCCTCCGGCCACCCGGGCCACACCACGTGGAGTATTTGGCGGGGGAGGTGGGGGTAGGCGCCCgaga                                                        >  3:79871/1‑134 (MQ=255)                                                                       aTTGCGCTGGCCATCCTCCAGCCATTTCGGCACCAGCAACAGCGAGAGCCC‑GCGGTTTGCCGGCGGCGCATCCCGCCACCGCGCCAACACCACGGGGAGTAATTAGCCGGGCAAGTCGTGTTAGCCCCCacc                                                          >  2:4337/1‑130 (MQ=255)                                                                       aTTGCGCTGGCCATCCTCCAGCCATTTCGGCACCAGCAACAGCGAGAGCCC‑GCGGTTTCCGGGCGGCGCATCCGGCAACCGGGCCCACACCAGGTGGAGTATGTCGGGGGGCAGGTGGTGGTCGCCGCCAGAGATg                                                      >  5:171642/1‑136 (MQ=255)                                                                       aTTGCGCTGGCCATCCTCCAGCCATTTCGGCACCAGCAACAGCGAGAGCCC‑GCGGTTGGCGGGCGGCGCCTCCGGCAACCGGGCCAACCCCAGGTGGTGTATGTCGCGGGTGAGGTGGTGTTCGCCGCCAgaga                                                        >  1:247502/1‑134 (MQ=255)                                                                       aTTGCGCTGGCCATCCTCCAGCCATTTCGGCACCAGCAACAGCGAGAGCCC‑GCGGTTGCCGGGCGGCGCATACGGCAACCCGGCCAACACCACGTGGTGTATGTCGCGGGGCAGGTGGTGTTCGCCGCCACAGAt                                                       >  6:304451/1‑135 (MQ=255)                                                                       aTTGCGCTGGCCATCCTCCAGCCATTTCGGCACCAGCAACAGCGAGAGCCC‑GCGGTTGCCCGGCGGCGCCACCGGCAACCGGGCCACCACCAGGTGGAGTATTTACCGGGTCAGGAAGTGTTAGCCCCCAgaga                                                        >  8:287797/1‑134 (MQ=255)                                                                         tGCGCTGGCCATCCTCCAGCCATTTCGGCACCAGCAACAGCGAGAGCCC‑GCGGTTGGCGGGCGGCGCCTCCGGCAACCGGGCCAACACCACGTGGAGTATGTCGCGGGTCAGGTCGTGTTAGCCGCCCGAGATg                                                      >  4:317220/1‑134 (MQ=255)                                                                         tGCGCTGGCCATCCTCCAGCCATTTCGGCACCAGCAACAGCGAGAGCCC‑GCGGTTGCCGGGCGGCGCATCCGGCAACCGGGCCAACACCAGGTGGAGTATGTCGCGGGTCAGGTCGTGTTCGCCGCCAGAGATg                                                      <  7:269958/134‑1 (MQ=255)                                                                            gcTGGCCATCCTCCAGCCATTTCGGCACCAGCAACAGCGAGAGCCC‑GCGGTTTCCGGGCGGCGCATCCGGCCACCGGGCCCACACCCCGGGGAGTATGTCGCGGGGGAGGTCGTGTTAGCCGCCAGAGATGAACAg                                                 >  6:141146/1‑136 (MQ=255)                                                                            gcTGGCCATCCTCCAGCCATTTCGGCACCAGCAACAGCGAGAGCCC‑GCGGTTGGCGGGCGGCGCCTCCGGCAACCGGGCCAACACCAGGTGGAGTATGTCGCGGGTCCGGTCGTGGTAGCCGCCAGAGATGAACa                                                  >  8:325763/1‑135 (MQ=255)                                                                            gcTGGCCATCCTCCAGCCATTTCGGCACCAGCAACAGCGAGAGCCC‑GCGGTTGGCGGGCGGCGCCGGCGGCCCCCGGGGCCACAACAGGTGGGGGATGTCGCGGGGCAGGTGGTGTTAGGCGGCCGAGATGCACAg                                                 >  6:403402/1‑136 (MQ=255)                                                                            gcTGGCCATCCTCCAGCCATTTCGGCACCAGCAACAGCGAGAGCCC‑GCGGTTGCCGGGGGGCGCATACGGCCAACGGGCCCACACCAGGTGGTGTTTGTGGCGGGTGAGGGCGTGTTGGCCGCCAGAGTTGTACAg                                                 >  6:196306/1‑136 (MQ=255)                                                                            gcTGGCCATCCTCCAGCCATTTCGGCACCAGCAACAGCGAGAGCCC‑GCGGTTGCCGGGCGGCGCATCCGGCAACCGGGGCAACACCAGGTGGAGTATGTCGCGGGTCAGGGCGTGTTCGCCGCCAGAGCTGAACa                                                  >  3:97611/1‑135 (MQ=255)                                                                            gcTGGCCATCCTCCAGCCATTTCGGCACCAGCAACAGCGAGAGCCC‑GCGGGTGGCGGGCGGCGCGGCCGGCCCCCCGGCCCACACCACGGGGGGTATGTGGCGGGGCCGGTGGGGGTCGCCGCCCGACATggcaa                                                  >  4:50100/1‑131 (MQ=255)                                                                              tGGCCATCCTCCAGCCATTTCGGCACCAGCAACAGCGAGAGCCC‑GCGGTTGGCCGGCGGCGCCTACGGCCACCCGGCCAACACCAGGTGGAGTATGTCGCGGGTCAGGTCGTGTTGGCCGCCCGAGCTGAACAGc                                                >  1:309042/1‑135 (MQ=255)                                                                              tGGCCATCCTCCAGCCATTTCGGCACCAGCAACAGCGAGAGCCC‑GCGGTTGCCGGGCGGCGCATCCGGCCACCGGGCCAACACCCGGGGGAGTATGTCGCGGGTCAGGGGGTGTTCGCCGCCAGAGATGAACAGcg                                               >  5:399126/1‑135 (MQ=255)                                                                                gCCATACTCCAGCCATTTCGGCACCAGCAACAGCGAGAGCCC‑GCGGTTGCCGGGCGGCGCATACGGCAAAAGGGCCAACACCAGGTGGAGGATTTAGCGGGGCCGGGAGGGTTACCCGCCAGCAATGCACAACta                                              >  4:109240/1‑134 (MQ=255)                                                                                 ccATCCTCCAGCCATTTCGGCACCAGCAACAGCGAGAGCCC‑GCGGTTGGCGGGGGGCGCATCCGGGAACCGGGGCCACACCAGGTGGGGTATTGGGCGGGGGAGGGGGGGTTAGCCGCCCGCGATGAACACCttgt                                            >  6:110275/1‑136 (MQ=255)                                                                                 ccATCCTCCAGCCATTTCGGCACCAGCAACAGCGAGAGCCC‑GCGGTTGGCGGGCGGCGCATCCCGCAACCGGGGCCACCCCAGGGGGTGTATGTAGCGGGGCAGGGGGTGTTGGCCGCCCGGGATGAACAGCgtg                                             >  1:274175/1‑135 (MQ=255)                                                                                 ccATCCTCCAGCCATTTCGGCACCAGCAACAGCGAGAGCCC‑GCGGTTGCCGGGCGGGGCCTCCGGCAACCCGGCCAACACCCGGGGGAGTATTTCGCGGGTCAGGGCGTGTTCTCCGCCAGAGCTGATCAGCgtg                                             >  7:192427/1‑135 (MQ=255)                                                                                   aTCCTCCAGCCATTTCGGCACCAGCAACAGCGAGAGCCC‑GCGGTTGGCGGGCGGGGCATCCGGCCACCGGGGCAACACCAGGTGGAGTATGTCGCGGGTGAGGTCGGGTTAGCCGCCAGAGATGAACAGCttgtt                                           >  7:119878/1‑135 (MQ=255)                                                                                   aTCCTCCAGCCATTTCGGCACCAGCAACAGCGAGAGCCC‑GCGGTTGGCGGGCGGCGCCTCCGGGCACCGGGGCACCCCCAGGTGGAGTATGTGGCGGGGGAGGTCGTGGTCGCCGCCCGAGAAGAACATCttggt                                           >  8:200436/1‑133 (MQ=255)                                                                                       tccAGCCATTTCGGCACCAGCAACAGCGAGAGCCC‑GCGGTTGCCGGGCGGCGCATCCGGCAACCGGGCCAACACCAGGTGGAGTATGTCGCGGGTCAGGTCGTGTTCGCCGCCAGAGATGAACAGCTTGTTGcc                                        <  8:171132/134‑1 (MQ=255)                                                                                                             aCAGCGAGAGCCC‑GCGGTTGCCGGGCGGCGCATCCGGCAACCGGGCCAACACCAGGTGGAGTATGTCGCGGGTCAGGGCGTGTTCGCCGCCAGAGATGAACAGCTTGTTGCCACTGACCGCGTAgccaccgtcgc                 <  4:348573/135‑1 (MQ=255)                                                                                                                             ggTTGCCGGGCGGCGCCTCCGGCCACCGGGCCCACACCAGGTGGAGTATGTCGCGGGTCAGGTCGGGTTCGCCGCCAGAGATGAACAGCTTGTTGCCACTGACCGCGTAGCCACCGGCGCCACCGTCGCCCAcgg  >  2:257456/1‑135 (MQ=255)                                                                                                                                  |                                                                                                                                  CCAGGCGTAGTGCACAGGTGGCGCTGCCCCGGATGCCCAGCTTATGTTCCAGGCCGTCGCAGTACAGTGTATTGCGCTGGCCATCCTCCAGCCATTTCGGCACCAGCAACAGCGAGAGCCC‑GCGGTTGCCGGGCGGCGCATCCGGCAACCGGGCCAACACCAGGTGGAGTATGTCGCGGGTCAGGTCGTGTTCGCCGCCAGAGATGAACAGCTTGTTGCCACTGACCGCGTAGCCACCGTCGCCACCGTCGCCCAGGG  >  ACB143/3785556‑3785813

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑