Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	ACB143	5,386,517	G→C	intergenic (‑526/+1125)	CJ019_05070 ← / ← nptII	hypothetical protein/neomycin phosphotransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	ACB143	5,386,517	0	G	C	100.0%	130.5 / NA	34	intergenic (‑526/+1125)	CJ019_05070/nptII	hypothetical protein/neomycin phosphotransferase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (17/17);  total (17/17)

CCTTTTTTATTGATAACAAAAATTGATCGAATTCTTTCATTTAAGACCCTAATATGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCCGT‑‑CGT‑‑‑CA‑‑GGATC‑‑A‑‑TCCGGGCGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTGCGGGTGCCAGGGCGTGCCCTTGG  >  ACB143/5386410‑5386604                                                                                                                       |                                                                                        ccTTTTTTATTGATAACAAAAATTGATCGAATTCTTTCATTTAAGACCCTAATATGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCAGT‑‑GGTG‑‑TAC‑GGTACAAA‑‑CCCCGACGCTAGCAACGCATGAGAAAGc                                                                 <  5:114183/136‑1 (MQ=255)          ttGATAACAAAAATTGATCGAATTCTTTCATTTAAGACCCTAATATGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCAGT‑‑GGTG‑‑TAC‑GGTACAAA‑‑CCCCGACGCTAGCAACGCATGAGAAAGCCCCCGGaa                                                         <  5:106402/135‑1 (MQ=255)           tGATAACAAAAATTGATCGAATTCTTTCATTTAAGACCCTAATATGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCAGT‑‑GGTG‑‑TAC‑GGTACAAA‑‑CCCCGACGCTAGCAACGCATGAGAAAGCCCCCGGaa                                                         <  3:244703/134‑1 (MQ=255)             aTAACAAAAATTGATCGAATTCTTTCATTTAAGACCCTAATATGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCAGT‑‑GGTG‑‑TAC‑GGTACAAA‑‑CCCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGa                                                       <  8:386166/134‑1 (MQ=255)                aCAAAAATTGATCGAATTCTTTCATTTAAGACCCTAATATGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCAGT‑‑GGTG‑‑TAC‑GGTACAAA‑‑CCCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCAcc                                                  >  6:150611/1‑136 (MQ=255)                  aaaaaTTGATCGAATTCTTTCATTTAAGACCCTAATATGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCAGT‑‑GGTG‑‑TAC‑GGTACAAA‑‑CCCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCt                                                 >  3:381243/1‑135 (MQ=255)                     aaTTCAGCGAATTCATTCATTTACGACCCTAATATGCCATCGCGGCCGGCTGGTCGACGACGGCGGGCTCAGTGGGGT‑‑‑AC‑‑GGAAC‑‑AACCCCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTcc                                              <  7:165306/135‑1 (MQ=255)                       ttGATCGAATTCTTTCATTTAAGACCCTAATATGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCAGT‑‑GGTG‑‑TAC‑GGTACAAA‑‑CCCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCgg                                            >  3:255237/1‑135 (MQ=255)                              aaTTCTTTCATTTAAGACCCTAATATGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCAGT‑‑GGTG‑‑TAC‑GGTACAAA‑‑CCCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCttt                                     >  8:208738/1‑135 (MQ=255)                                      cATTTAAGACCCTAATATGACATCTCGGCCGGCTTGGCGACGACGGCGGTCTCAGT‑‑GGTG‑‑TAC‑GGTACAAA‑‑CCCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTAtt                               <  1:277751/133‑1 (MQ=255)                                        tttACGCCCCAAATATTCCATCTCGCCCGGCTTGTCCCCGACGGCGGGCTCAGT‑‑GTT‑‑‑GAACGGAAC‑‑AACCCCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTgcgc                           <  1:212383/135‑1 (MQ=255)                                        tttAAGACCCTAATATGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCAGT‑‑GGTG‑‑TAC‑GGTACAAA‑‑CCCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCt                          <  5:273103/136‑1 (MQ=255)                                        tttAAGACCCTAATATCACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCAGT‑‑GGTG‑‑TAC‑GGTACAAA‑‑CCCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTgcgc                           <  2:188076/135‑1 (MQ=255)                                             gcccatAATATGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCTGT‑‑GGTG‑‑TAC‑GGTACAAA‑‑CCCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTGCggg                     <  6:230161/131‑1 (MQ=255)                                               cccTAAAATCACAGCTCCGCCGGCGTTCCGACGACGGCGGGCTCAGT‑‑CGTG‑‑TAC‑GGTACAAA‑‑CCCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTGCGGGt                    <  7:168325/135‑1 (MQ=255)                                                    atatGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCAGT‑‑GGTG‑‑TAC‑GGTACAAA‑‑CCCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTGCGGGTGCCAgg              >  6:380789/1‑136 (MQ=255)                                                     tatGCCATCTCGGCGGGCTTGTCGACGACGGCGGTCTCAGTGGGGT‑‑‑CC‑‑GGTAC‑‑AACCCCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTGCGGGTGCCAgg              <  7:336467/135‑1 (MQ=255)                                                     tatGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCAGT‑‑GGTG‑‑TAC‑GGTACAAA‑‑CCCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTTCGCTGCGGGTGCCAggg             >  6:3042/1‑136 (MQ=255)                                                     tatGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCAGT‑‑GGTG‑‑TAC‑GGTACAAA‑‑CCCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTGCGGGTGCCAggg             >  6:348404/1‑136 (MQ=255)                                                     tatGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCAGT‑‑GGTG‑‑TAC‑GGTACAAA‑‑CCCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTGCGGGTGCCAgg              >  4:394029/1‑135 (MQ=255)                                                     tatGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCAGT‑‑GGTG‑‑TAC‑GGTACAAA‑‑CCCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTGCGGGTGCCAgg              <  8:116564/135‑1 (MQ=255)                                                     tatGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCAGT‑‑GGTG‑‑TAC‑GGTACAAA‑‑CCCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTGCGGGTGCCAgg              <  7:656/135‑1 (MQ=255)                                                     tatGACATCTCGGCCGGCTTGTCGACGACGGCGGGCTCAGT‑‑GGTG‑‑TAC‑GGTACAAA‑‑CCCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTGCGGGTGCCAgg              <  7:20849/135‑1 (MQ=255)                                                     tatGACATCTCGGCCGGCTTGGCGACGACGGCGGTCTCAGT‑‑GGTG‑‑TAC‑GGTACAAA‑‑CCCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTGCGGGTGCCAgg              <  2:374337/135‑1 (MQ=255)                                                      atGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCAGT‑‑GGTG‑‑TAC‑GGTACAAA‑‑CCCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCGTTTTTATTGCGCTGCGGGTGCCAGGGc            >  5:250902/1‑136 (MQ=255)                                                             ctcGGCCGGCTTGTCGACGACGGCGGTCTCAGT‑‑GGTGTACG‑‑GTACA‑‑A‑‑CCCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTGCGGGTGCCAGGGCGTGCCCtt    >  5:205721/1‑136 (MQ=255)                                                             ctcGGCCGGCTTGTCGACGACGGCGGTCTCAGT‑‑GGTG‑‑TAC‑GGTACAAA‑‑CCCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTGCGGGTGCCAGGGCGTGccc      >  2:226261/1‑135 (MQ=255)                                                             ctcGGCCGGCTTGTCGACGACGGCGGTCTCAGT‑‑GGTG‑‑TAC‑GGTACAAA‑‑CCCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTGCGGGTGCCAGGGCGTGccc      >  1:43200/1‑135 (MQ=255)                                                             ctcGGCCGGCTTGTCGACGACGGCGGTCTCAGT‑‑GGTG‑‑TAC‑GGTACAAA‑‑CCCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTGCGGGTGCCAGGGCGTGCCCt     >  6:163699/1‑136 (MQ=255)                                                                ggcgggcTTGTCGACGACGGCGGTCTCAGT‑‑GGTG‑‑TAC‑GGTACAAA‑‑CCCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTGCGGGTGCCAGGGCGTGCCCTTg   >  7:361002/1‑135 (MQ=255)                                                                ggccggcTTGTCGACGACGGCGGTCTCAGT‑‑GGTG‑‑TAC‑GGTACAAA‑‑CCCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTGCGGGTGCCAGGGCGTGCCCTTg   >  3:366226/1‑135 (MQ=255)                                                                ggccggcTTGTCGACGACGGCGGTCTCAGT‑‑GGTG‑‑TAC‑GGTACAAA‑‑CCCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGAACACCTTCCGGGGGCTTTTTTATTGCGCTGCGGGTGCCAGGGCGTGCCCTTg   >  7:144706/1‑135 (MQ=255)                                                                 gccggcTTGTCGACGACGGCGGTCTCAGT‑‑GGTG‑‑TAC‑GGTACAAA‑‑CCCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTGCGGGTGCCAGGGCGTGCCCTTgg  >  8:233242/1‑135 (MQ=255)                                                                 gccggcTTGTCGACGACGGCGGTCTCAGT‑‑GGTG‑‑TAC‑GGAACAAA‑‑CCCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTGCGGGTGCCAGGGCGTGCCCtt    <  4:118941/133‑1 (MQ=255)                                                                                                                       |                                                                                        CCTTTTTTATTGATAACAAAAATTGATCGAATTCTTTCATTTAAGACCCTAATATGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCCGT‑‑CGT‑‑‑CA‑‑GGATC‑‑A‑‑TCCGGGCGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTGCGGGTGCCAGGGCGTGCCCTTGG  >  ACB143/5386410‑5386604

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑