Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1433,440,3310TG54.5% ‑2.7 / 10.9 24A19A (GCT→GCG) CJ019_03266hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (9/1);  new base G (8/4);  total (19/5)
Fisher's exact test for biased strand distribution p-value = 3.23e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.46e-01
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

AAACGTATCAATGGTGGTAGCAACGGTCTGGAGGATCGCAAGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCAGCGCTGGCGGCGCCCGGGCAGCCTGGGTGTGGCAGGCCAACGCCTATAAGGCGCAGCTCGCTGATCAGGCCGATGACTTCGGCAAGCAGTTGGCGGAGAAGGATCGCGCGCACGGTCTGGAGCGGGAGAAGGCTGCA  >  ACB143/3440201‑3440463
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aaaCGTATCAATGGTGGTAGCAACGGTCTGGAGGATCGCAAGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCAGCGCTggcggc                                                                                                                                >  3:92195/1‑137 (MQ=255)
                gtAGCAACGGTCTGGAGGATCGCAAGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCGCGCAGGCGGCGCCCGCGCGGCCTcgg                                                                                                                >  1:268225/1‑137 (MQ=255)
                   gCAACGGTCTGGAGGATCGCAAGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCAGCGCTGGCGGCGCCCCGGCAGCCGGGgtgt                                                                                                             >  5:73552/1‑137 (MQ=255)
                          gcTGGAGCACCGCAAGGCCCTCAACCCGCCCGCGGGCGGGGTGCGGCAGGGCGCGCTGGGCCCCCCCGCCGGGGCGGGGTGGCGCGGCGGCGGGCCCCCCGCGCGGGCGGCGCCCGGGCAGCCTGGGTGTGGCAGGc                                                                                                      <  8:191742/136‑1 (MQ=255)
                                           cccTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCGCGCGGGCGGCGCCCGGGCAGCCTGGGGGTGGCAGGCCCACCCCGATAAGgcgc                                                                                     >  8:109586/1‑137 (MQ=255)
                                                 aCAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCGCGCTGGCGGCGCCCGGGCCGCCGGGGGGTGGCAGGCCCACGCCAATAAGGCGCAGCTCg                                                                               >  3:85535/1‑137 (MQ=255)
                                                        agcgCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCGCGCGGGCGGCGCCCCGGCCGCCTGGGGGTGGCCGGCCACCGCCGATAAAGCGCAACGCGCGGagcc                                                                        >  4:127709/1‑134 (MQ=255)
                                                           gcTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCCCGCGGGCGGCGCCCCGGCCACCTGGGTGTGGGACGCCAACCCCTATAAGTCGCAGCTAGCAGAtaaaga                                                                     >  6:307388/1‑132 (MQ=255)
                                                           gcTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCAGCGCGGGCGGCGCCCGGGCAGCCTGGGTGTGGCAGGCCAACGCCTATAAGGCGCAGCTCGCGGATCAggg                                                                     >  8:44423/1‑136 (MQ=255)
                                                             tCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCGCGCTGGCGGCGCCCGGGCCGCCGGGGTGTGGCAGGCCCACGCCTATAAGGCGCCGCTCGCTGATCAGGCCg                                                                   >  5:221879/1‑137 (MQ=255)
                                                             tCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCGCGCTGGCGGCGCCCGGGCAGCCGGGGGGTGGCAGGCCAACGCCTATAAGGCGCAGCTCGCTGCTCAGGCCg                                                                   >  5:249287/1‑137 (MQ=255)
                                                             tCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCCCCAGCGCTGGCGGCGCCCCGGGCGCCAGGGTGTGGCAGGCCAAAGCCTATAAGGCGCAGCTCGCTGATCAGGccc                                                                   >  4:217358/1‑136 (MQ=255)
                                                                                gcTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGGCGCCCGCCCGGGCGGGGCCCGGGCCGCCGGGGGGTGGCAGGCCCACCCCGATAAGGGGCAGCGCGCGGATCAGGCCGCTGACTTCGGCAAGCAggt                                                >  2:239651/1‑135 (MQ=255)
                                                                                gcTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCGCGCGGGCGGCGCCCGGGCCGCCGGGGTGTGGGAGGCCACCCCCGATAAGGCGCAGCTCGCTGATCAGGCCGATGACGTCGGCAAGCAGtt                                                >  3:214550/1‑137 (MQ=255)
                                                                                gcTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCCCGCGGGCGGCGCCCGGGCCGCCGGGGGGTGGGCGGCCAACGCCAATAATGCGCAGCTCGCGGCTCAGGCCGCGGACGTACGCCAGCAGtt                                                >  6:19001/1‑137 (MQ=255)
                                                                                gcTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCAGCGCTGGCGGCGCCCGGGCCGCCGGGGGGTGGCAGGCCAACGCCGATAAGGCGCAGCTCGCTGATCAGGCCGATGTCTTCGGCAAGCAGtt                                                >  7:64482/1‑137 (MQ=255)
                                                                                 ttggggCCCCCCGCGTGGGCGCGGGGGGGCGGCGGGGGCCCGCCCGGGGGGGGGGCGCCCGGGCAGCCTGGGTGTGGCAGGCCAACGCCTATAAGGCGCAGCTCGCTGATCAGGCCGATGACTTCGGCAAGCAGTTg                                               <  8:165654/134‑1 (MQ=255)
                                                                                      gCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCAGCGCTGGCGGCGCCCCGGCCGCCTGGGTGTGGCAGGCCAACCCCGATAAGGCGCCGCTCGCTGGTCAGGCCGATGACTTAGGGAAGCACTTGGCggg                                          >  5:323094/1‑136 (MQ=255)
                                                                                            cGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCGCGCGGGCGGCGCCCGGGCAGCCGGGGGGGGGGCGGCCCACGCCGATAAGGCGCAGCGCGCGGTTAAGGCCGATGACTTCGGCCAGCAGGTGGCGGCGAAggc                                    >  8:17582/1‑136 (MQ=255)
                                                                                                       gggggCGCTGCGGCGGGCCGCGAGCGCGGCCGGCGCCCGGGCAGCCTGGGTGTGGCAGGCCAACGCCTATAAGGCGCAGCTCGCTGATCAGGCCGATGACTTCGGCAAGCAGTTGGCGGAGAAGGATCGCGCGCACg                         <  5:131876/134‑1 (MQ=255)
                                                                                                        gTGGCGCTGCTGCTGGCCGCCAGCGCTGGTGGCGCCCGGGCAGCCTGGgtgt                                                                                                             <  7:299336/52‑1 (MQ=255)
                                                                                                        gTGGCGCTGCTGCTGGCCGCCAGCGCTGGTGGCGCCCGGGCAGCCTGGgtgt                                                                                                             >  8:299336/1‑52 (MQ=255)
                                                                                                                       gccgccGGCGCGGGCGGCGCCCGGGCAGCCTGGGTGTGGCAGGCCAACGCCTATAAGGCGCAGCTCGCTGATCAGGCCGATGACTTCGGCAAGCAGTTGGCGGAGAAGGATCGCGCGCACGGTCTGGAGCGGGAGaa         <  1:186175/137‑1 (MQ=255)
                                                                                                                         cgcccccgcgGGGGGCGCCCCGGCCCCCTGGGTGTGGGAGGCCAACCCCGATAAGGCGCAGCTCGCTGATCAGGCCGAAGACTGCGGCAAGCAGTTGGCGGAGAACGATAGCGCCCCCGGTCTGGTGGGGGAGAAgg       >  4:282503/7‑137 (MQ=255)
                                                                                                                              ccgcAGGCGGCGCCCGGGCCGCCAGGGTGTGGCAGGCCAAACCCAATAAGTCGCAGCTCGCTGATCAGGCCCAGGACTTAGGCAAGCAATTGGCGGCGAAGGATCGCGCGCACGGGCTGGAGCGGGGGAAGGGTgcc  >  6:47696/2‑136 (MQ=255)
                                                                                                                                  |                                                                                                                                    
AAACGTATCAATGGTGGTAGCAACGGTCTGGAGGATCGCAAGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCAGCGCTGGCGGCGCCCGGGCAGCCTGGGTGTGGCAGGCCAACGCCTATAAGGCGCAGCTCGCTGATCAGGCCGATGACTTCGGCAAGCAGTTGGCGGAGAAGGATCGCGCGCACGGTCTGGAGCGGGAGAAGGCTGCA  >  ACB143/3440201‑3440463

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑