Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1434,028,4480GA25.9% 43.5 / 10.4 34intergenic (‑117/‑130)CJ019_03802/mcpU_3hypothetical protein/Methyl‑accepting chemotaxis protein McpU
Reads supporting (aligned to +/- strand):  ref base G (18/2);  new base A (0/7);  total (18/16)
Fisher's exact test for biased strand distribution p-value = 4.05e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

GCGGCGATCACTGCATTCATGGTTGGCTTACCTCATTACGACAGAAAAAAGCGCGAACTCTGAAGCAGTGGGCGGCAGATGTCAAAAGCGCCTTGATGCGGCGCGTTAACGGCGGACAAATTTAAATTG‑AATGTTTGAATAAAGAAAAAACGGCAGGGGCCGATATTGTGGGAGGCAGCGAGGTGCCTGGCCGGAGCGCTTTGGCACCTCTCGGCCCCACCGCAGATACAGCCATAACCCTTATTGCCAAAAAGGGATCCGCCC  >  ACB143/4028312‑4028575
                                                                                                                                         |                                                                                                                               
gcggcgATCACTGCATTCATGGTTGGCTTACCTCATTACGACAGAAAAACGCGCGAACTCTGAAGCAGTGGGCGGCAGATGTCAAAAGCGCCTTGATGCGGCGCGTTAACGGCGGACAAATTTAAATTG‑ATTGTTTg                                                                                                                                 >  7:89185/1‑137 (MQ=255)
gcggcgATCACTGCATTCATGGTTGGCTTACCTCATTACGACAGAAAAAAGCGCGAACTCTGAAGCAGTGGGCGGCAGATGTCAAAAGCGCCTTGATGCGGCTCGTTAACGGCGGACAAATTTAAATTG‑AATGTTTg                                                                                                                                 >  7:154233/1‑137 (MQ=255)
gcggcgATCACTGCATTCATGGTTGGCTTACCTCATTACGACAGAAAAAAGCGCGAACTCTGAAGCAGTGGGCGGCAGATGTCAAAAGCGCCTTGATGCGGCGCGTTAACGGCGGACAAATTTAAATTG‑AATGTTTg                                                                                                                                 >  5:59331/1‑137 (MQ=255)
                          cTTACCTCATTACGACAGAAAAAAGCGCGAACTCTGAAGCAGTGGGCGGCAGATGTCAAAAGCGCCTTGATGCGGCGCGTTAACGGCGGACAAATTTAAATTG‑AATGTTTGAATAAAGAAAAAACGGCAGGGGCCGa                                                                                                       >  1:270632/1‑137 (MQ=255)
                             aCCTCATTACGACAGAAAAAAGCGCGAACTCTGAAGCAGTGGGCGGCAGATGTCAAAAGCGCCTTGATGCGGCGCGTTAACGGCGGACAAATTTAAATTG‑AATGTTTGAATAAAGAAAAAACGGCAGGGGCCGatat                                                                                                    >  5:313341/1‑137 (MQ=255)
                                tCATTACGACAGAAAAAAGCGCGAACTCTGAAGCAGTGGGCGGCAGATGTCAAAAGCGCCTTGATGCGGCGCGTTAACGGCGGACAAATTTAAATTG‑AATGTTTGAATAAAGAAAAAACGGCAGGGGCCGATATtgt                                                                                                 >  4:30721/1‑137 (MQ=255)
                                       gACAGAAAAAAGCGCGAACTCTGAAGCAGTGGGCGGCAGATGTCAAAAGCGCCTTGATGCGGCGCGTTAACGGCGGACAAATTTAAATTG‑AATGTTTGAATAAAGAAAAAACGGCAGGGGCCGATATTGTGGGAGGc                                                                                          >  1:105405/1‑137 (MQ=255)
                                           gAAAAAAGCGCGAACTCTGAAGCAGTGGGCGGCAGATGTCAAAAGCGCCTTGATGCGGCGCGTTAACGGCGGACAAATTTAAATTG‑AATGTTTGAATAAAGAAAAAACGGCAGGGGCCGATATTGTGGGAGGCAGCg                                                                                      >  7:248117/1‑137 (MQ=255)
                                            aaaaaaGCGCGAACTCTGAAGCAGTGGGCGGCAGATGTCAAAAGCGCCTTGATGCGGCGCGTTAACGGCGGACAAATTTAAATTG‑AATGTTTGAATAAAGAAAAAACGGCAGGGGCCGATATTGTGGGAGGCAGCGa                                                                                     >  2:326483/1‑137 (MQ=255)
                                            aaaaaaGCGCGAACTCTGAAGCAGTGGGCGGCAGATGTCAAAAGCGCCTTGATGCGGCGCGTTAACGGCGGACAAATTTAAATTG‑AATGTTTGAATAAAGAAAAAACGGCAGGGGACGATATTGTGGGAGGCAGCgc                                                                                     >  8:71729/1‑136 (MQ=255)
                                               aaaGCGCGAACTCTGAAGCAGTGGGCGGCAGATGTCAAAAGCGCCTTGATGCGGCGCGTTAACGGCGGACAAATTTAAATTG‑AATGTTTGAATAAAGAAAAAACGGCAGGGGCCGATATTGTGGGAGGCAGCGAGGt                                                                                  >  2:64158/1‑137 (MQ=255)
                                               aaaGCGCGAACTCTGAAGCAGTGGGCGGCAGATGTCAAAAGCGCCTTGATGAGGCGCTTTAACGGCGGACAAATTTAAATTG‑AATGTTTGAATAAAGAAAAAACGGCAGGGGCCGATATTGTGTGAGCCAGCGAGGt                                                                                  >  5:278996/1‑137 (MQ=255)
                                                                gccgggggCGGCATTTGTCAAGCGGGCCTGGTTGCGGCGGGTAACCGGCGGATAAATTAAAATTG‑ATTGTTTAAATATCGAAAACACGGCAGGGGCCGATATTGTGGGAGGCAGCGAGGTGCCTGGCCGGAGCGCtt                                                                 <  8:270702/132‑1 (MQ=255)
                                                                gCAGTGGGCGGCATATGTCAAACGCGCCTTGAGGGGGCGCTTTACCGGCGCAAAAATTTAAATTG‑AATGTTTTAAAAAAAAAAACACGGCAGGGGCCGATATTGTGGGAGGCAGCGAGGTGCCTGGCCGGAGCGCtt                                                                 <  4:249608/137‑1 (MQ=255)
                                                                       gcggcAGATGTCAAAAGCGCCTTGATGCGGCGCGTTAACGGCGGACAAATTTAAATTG‑AATGTTTGAATAAAGAAAAAACGGCAGGGGCCGATATTGTGGGAGTCAGCGAGTTGCCTGGCCGGAGCGCTTTGGCAcg                                                          >  1:188055/1‑136 (MQ=255)
                                                                       gcggcAGATGTCAAAAGCGCCTTGATGCGGCGCGTTAACGGCGGACAAATTTAAATTG‑AATGTTTGAATAAAGAAAAAACGGCAGGGGCCGATATTGTGGGAGGCAGCGAGGTGCCTGGCCGGAGCGCTTTGGCAcc                                                          >  1:41127/1‑137 (MQ=255)
                                                                       gcggcAGATGTCAAAAGCGCCTTGATGCGGCGCGTTAACGGCGGACAAATTTAAATTG‑AATGTTTGAATAAAGAAAAAACGGCAGGGGCCGATATTGTGGGAGGCAGCGAGGTGCCTGGCCGGAGCGCTTTGGCAcc                                                          >  7:274005/1‑137 (MQ=255)
                                                                         ggcAGATGTCAAAAGCGCCTTGATGCGGCGCGTTAACGGCGGACAAATTTAAATTG‑AATGTTTGAATAAAGAAAAAACGGCAGGGGCCGATATTGTGGGAGGCAGCGAGGTGCCTGGCCGGAGCGCTTTGGCACctc                                                        >  5:100245/1‑137 (MQ=255)
                                                                                  aaaacGCGCCTTGATGCGGCGCTTTAACGGCGCAAAAATTTAAATTT‑AATGTTTAAAAAAACAAAAAACGGCAGGGGCCGATATTGTGGGAGGCAGCGAGGTGCCTGGCCGGAGCGCTTTGGCACCTCTCGGCCCCa                                               <  4:151798/136‑1 (MQ=255)
                                                                                   aaaaGCGCCTTGATGCGGCGCGTTAACGGCGGACAAATTTAAATTG‑AATGTTTGAATAAAGAAAAAACGGCAGGGGCCGATATTGTGGGAGGCAGCGAGGTGCCTGGCCGGAGCGCTTTGGCACCTCTCGGCCCCAc                                              >  6:36990/1‑137 (MQ=255)
                                                                                         gcCTTTATGCGGCTGTTTAACGCCGTAAAAATTTAAATTT‑AATGTTTAAAAAAACAAAACACGGCAGGGGCCGATATTGTGGGAGGCAGCGAGGTGCCTGGCCGGAGCGCTTTGGCACCTCTCGGCCCCACCGCAGa                                        <  5:249419/137‑1 (MQ=255)
                                                                                         gcCTTGATGGGGCGCGTTAACGGCGGACAAATTTAAATTG‑AATGTTTTAATAATGAAAACACGGCAGGGGCCGATATTGTGGGAGGCAGCGAGGTGCCTGGCCGGAGCGCTTTGGCACCTCTCGGCCCCACCGCAGa                                        <  8:237470/137‑1 (MQ=255)
                                                                                         gcCTTGATGCGGCGCGTTAACGGCGGAAAAATTTAAATTT‑AATGTTTGAATAAAGAAAACACGGCAGGGGCCGATATTGTGGGAGGCAGCGAGGTGCCTGGCCGGAGCGCTTTGGCACCTCTCGGCCCCACCGCAGa                                        <  8:237468/137‑1 (MQ=255)
                                                                                         cccTTGTGGCGGCGCTTTAACGGCGTAAAATTTAAAATTT‑ATTTTTTAAATAAAAAAAACACGGCAGGGGCCGATATTGTGGGAGGCAGCGAGGTGCCTGGCCGGAGCGCTTTGGCACCTCTCGGCCCCACCGCAGa                                        <  5:174938/136‑1 (MQ=255)
                                                                                         cccTTCATGCGGCGCTTAACCGCCGCAAAAATAAAAATTG‑AATGTTTAAAAAAACAAAAAACGGCAGGGGCCGATATTGTGGGAGGCAGCGAGGTGCCTGGCCGGAGCGCTTTGGCACCTCTCGGCCCCACCGCAGa                                        <  1:79823/136‑1 (MQ=255)
                                                                                              gATGCGGCGCGTTAACGGCGGACAAATTTAAATTG‑AATGTTTGAATAAAGAAAAAACGGCAGGGGCCGATATTGTGGGAGGCAGCGAGGTGCCTGGCCGGAGCGCTTTGGCACCTCTCGGCCCCACCGCAGATACAg                                   >  1:86384/1‑137 (MQ=255)
                                                                                                tGCGGCGGTTAAAGGGCGTAAAAATTTAAATTT‑ATTTTTTTAATAAACAAAAAACGGCAGGGGCCGATATTGTGGGAGGCAGCGAGGTGCCTGGCCGGAGCGCTTTGGCACCTCTCGGCCCCACCGCAGATACAGcc                                 <  1:25606/137‑1 (MQ=255)
                                                                                                tGCGGCGCTTTAACGGGGGAAAAATTTAATTTG‑AATGTTTGAAAAAATAAAACACGGCAGGGGCCGATATTGTGGGAGGCAGCGAGGTGCCTGGCCGGAGCGCTTTGGCACCTCTCGGCCCCACCGCAGATACAGcc                                 <  5:263532/137‑1 (MQ=255)
                                                                                                 gcggggCGTTACCGGCGCA‑AAATTTAAAATGCATTGTTTCAAAACAAAAACCACGGCAGGGGCCGATATTGTGGGAGGCAGCGAGGTGCCTGGCCGGAGCGCTTTGGCACCTCTCGGCCCCACCGCAGATACAGCCa                                <  4:123163/137‑1 (MQ=255)
                                                                                                       ggTTAACGGCTCACAAATTAAAATTT‑AATGTTTAAATAAATAAAACACGGCAGGGGCCGATATTGTGGGAGGCAGCGAGGTGCCTGGCCGGAGCGCTTTGGCACCTCTCGGCCCCACCGCAGATACAGCCATAAccc                          <  7:303738/136‑1 (MQ=255)
                                                                                                       cgTTAACGGCGGACAAATTTAAATTG‑AATGTTTGAATAAAGAAAAAACGGCAGGGGCCGATATTGTGGGAGGCAGCGAGGTGCCTGGCCGGAGCGCTTTGGCACCTCTCGGCCCCACCGCAGATACAGCCATAAccc                          >  5:132551/1‑137 (MQ=255)
                                                                                                        tttAACGGCGGAAAAATTTAAATTG‑AATGTTTTAATAAAGAAAAAACGGCAGGGGCCGATATTGTGGGAGGCAGCGAGGTGCCTGGCCGGAGCGCTTTGGCACCTCTCGGCCCCACCGCAGATACAGCCATAACCCt                         <  8:274005/136‑1 (MQ=255)
                                                                                                        gTTAACGGCGGACAAATTTAAATTG‑AATGTTTGAATAAAGAAAAAACGGCAGGGGCCGATATTGTGGGAGGCAGCGAGGTGCCTGGCCGGAGCGCTTTGGCACCTCTCGGCCCCACCGCAGATACAGACATAACCCt                         >  3:64873/1‑137 (MQ=255)
                                                                                                            aCGGCGTAAAAATTTAAATTT‑ATTTTTTTAAAAAATAAAACACGGCAGGGGCCGATATTGTGGGAGGCAGCGAGGTGCCTGGCCGGAGCGCTTTGGCACCTCTCGGCCCCACCGCAGATACAGCCATAACCCTTAtt                     <  3:270908/137‑1 (MQ=255)
                                                                                                               gcggACAAATTTAAATTT‑AATGTTTTAATAAAGAAAACACGGCAGGGGCCGATATTGTGGGAGGCAGCGAGGTGCCTGGCCGGAGCGCTTTGGCACCTCTCGGCCCCACCGCAGATACAGCCATAACCCTTATTGcc                  <  6:92478/137‑1 (MQ=255)
                                                                                                                    cAAATTTAAATTG‑AATGTTTGAATAAAGAAAAAACGGCAGGGGCCGATATTGTGGGAGGCAGCGAGGTGCCTGGCCGGAGCGCTTTGGCACCTCTCGGCCCCACCGCAGATACAGCCATAACCCTTATTGCCaaaaa             >  5:110258/1‑137 (MQ=255)
                                                                                                                                ttaaTTTTTAAATAAAAAAAACACGGCAGGGGCCGATATTGTGGGAGGCAGCGAGGTGCCTGGCCGGAGCGCTTTGGCACCTCTCGGCCCCACCGCAGATACAGCCATAACCCTTATTGCCAAAAAGGGATCCGccc  <  1:260889/135‑1 (MQ=255)
                                                                                                                                         |                                                                                                                               
GCGGCGATCACTGCATTCATGGTTGGCTTACCTCATTACGACAGAAAAAAGCGCGAACTCTGAAGCAGTGGGCGGCAGATGTCAAAAGCGCCTTGATGCGGCGCGTTAACGGCGGACAAATTTAAATTG‑AATGTTTGAATAAAGAAAAAACGGCAGGGGCCGATATTGTGGGAGGCAGCGAGGTGCCTGGCCGGAGCGCTTTGGCACCTCTCGGCCCCACCGCAGATACAGCCATAACCCTTATTGCCAAAAAGGGATCCGCCC  >  ACB143/4028312‑4028575

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑