| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | ACB143 | 4,425,070 | 0 | G | C | 36.8% | 8.3 / 10.2 | 19 | intergenic (‑29/‑89) | CJ019_04200/CJ019_04201 | hypothetical protein/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base G (9/3); new base C (0/7); total (9/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.10e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GTCGAGCTGCGCAGTCGGCTTGCCGCCACGCGCCGTTTCCATGGTCGCAGAAGCACAACCGGCCAGCATCAGCACCGCAGCCAACGGCAGAATCGCAC‑GCATGTTCCATCCTCGGGGTCGCAAAAAAGAAGGCGACTCTAGCACTGGCAGGCATCCCGAGTGAATTGCCGCGCACTTGCCGCTGTCGCAGATAAAACATGCCATCACGAGGAACCCATCATGAAACTGCACTATCTGCTACTCGCCGCCCTGCCTTGC > ACB143/4424940‑4425197 | gTCGAGCTGCGCAGTCGGCTTGCCGCCACGCGCCGTTTCCATGGTCGCAGAAGCACAACCGGCCAGCATCAGCACCGCAGCCAACGGCAGAATCGCAC‑GCATGTTCCATCCTCGGGGTCGCAAAAAAGAAGGCGAct > 6:168073/1‑137 (MQ=255) ccgccACGCGCCGTTTCCATGGTCGCAGAAGCACAACCGGCCAGCATCAGCACCGCAGCCAACGGCAGAATCGCAC‑GCATGTTCCATCCTCGGGGTCGCAAAAAAGAAGGCGACTCTAGCACTGGCAGGCATCCCGa > 7:99790/1‑137 (MQ=255) gccACGGGCTGTTTCGTGGGTCGAACATTCAAACCCGGCCAGCATCCGCACCGCACCCCACGCCACAATCGCAC‑GTATGTTCCCCCGTGGGGGGCAAAAAAAACAAGGCGACTCTAGCACTGGCAGGCATCCCGAGt < 6:141956/137‑1 (MQ=255) gcgcCGTTTCCATGGTCGCAGAAGCACAACCGGCCAGCATCAGCACCGCAGCCAACGGCAGAATCGCAC‑GCATGTTCCATCCTCGGGGTCGCAAAAAAGAAGGCGACTCTAGCACTGGCAGGCATCCCGAGTGAAtt > 1:123669/1‑137 (MQ=255) gcCGTTTCCATGGTCGCAGAAGCACAACCGGCCAGCATCAGCACCGCAGCCAACGGCAGAATCGCAC‑GCATGTTCCATCCTCGGGGTCGCAAAAAAGAAGTCGACTCTAGCACTGGCAGGCATCCCGAGTGAATTGc > 8:235122/1‑137 (MQ=255) ccttttCCATGGCCGCAGAAGCACAACCGGCCTCCATCCGCACCGCCGCCAAGGGCATAAGCGCCC‑GCATGTTCCATCCCGGGGGTCCCAAAAAACACGGCGACTCTAGCACTGGCAGGCATCCCGAGTGAATTGcc < 5:146678/134‑1 (MQ=255) aaGCACAACCGGCCAGCATCAGAACCGCAGCCAACTGCAGAATCGCAC‑GCATGTTCCATCCTCGGGGTCGCAAAAAAGAAGGCGACTCTAGCACTGGCAGGCATCCCGAGTGAATTGCCGCGCACTTGCCGCTGTCg > 1:43037/1‑137 (MQ=255) ccGGCCAGCAACACCACCCCCCCCACCGCCAGAAAC‑CACGGAATTTTCAATCCGCGGGGCCCAAAAAAATACGGCGACTCTAGCACTGGCAGGCATCCCGAGTGAATTGCCGCGCACTTGCCGCTGTCGCAGATaaa < 4:249797/137‑1 (MQ=255) ggCCAGCATCACCACCGCAGCCAACGGCATAATCGCAC‑GCATTTTCCATCCTCGGGGTCGCAAAAAAGAACGCGACTCTAGCACTGGCAGGCATCCCGAGTGAATTGCCGCGCACTTGCCGCTGTCGCAGATAAAAc < 7:28309/137‑1 (MQ=255) aGCATCACCACCGCACCCACCGGCATAATCCCCG‑GAATGTTCCTGCCGCGGGGGCCCAAAAAACACCGCGACTCTAGCACTGGCAGGCATCCCGAGTGAATTGCCGCGCACTTGCCGCTGTCGCAGATAAAACATGc < 7:224726/137‑1 (MQ=255) aCCGAAGCCACCGGCATAATCGCAC‑GAATTTTCCATCCTGGGGTTCCAAAAAAACAACGCGACTCTAGCACTGGCAGGCATCCCGAGTGAATTGCCGCGCACTTGCCGCTGTCGCAGATAAAACATGCCATCACGAg < 8:236561/137‑1 (MQ=255) ccgccAGAAGCGCAC‑GCATGTACAATCCGCGGGGTCGAAAAAAACACCGCGACTCTAGCACTGGCAGGCATCCCGAGTGAATTGCCGCGCACTTGCCGCTGTCGCAGATAAAACATGCCATCACGAGGAACCcatca < 5:76564/133‑1 (MQ=255) aCGGCAGAATCGCAC‑GCATGTTCCATCCTCGGGGTCGCAAAAAAGAAGGCGACTCTAGCACTGGCAGGCATCCCGAGTGAATTGCCGCGCACTTGCCGCTGTCGCAGATAAAACATGCCATCACGAGGAACCcatca > 1:177943/1‑137 (MQ=255) aaTCCCAC‑GCATTTACCAGCCGGGGGGTCCAAAAAAAGACCGCGACTCTAGCACTGGCAGGCATCCCGAGTGAATTGCCGCGCACTTGCCGCTGTCGCAGATAAAACATGCCATCACGAGGAACCCATCATGAAACt < 3:115213/137‑1 (MQ=255) accccc‑gcaTGTACCATCCTCGGGGACGAAAAAAACAACGCGACTCTAGCACTGGCAGGCATCCCGAGTGAATTGCCGCGCACTTGCCGCTGTCGCAGATAAAACATGCCATCACGAGGAACCCATCATGAAACTGc < 3:156726/132‑1 (MQ=255) gcaTGTTCCATCCTCGGGGTCGCAAAAAAGAAGGCGACTCTAGCACTGGCAGGCATCCCGAGTGAATTGCCGCGCACTTGCCGCTGTCGCAGATAAAACATGCCATCACGAGGAACCCATCATGAAACTGCACTATc > 8:46217/1‑137 (MQ=255) tCCATCCGCGGGGTCCAAAAAAAGACCGCGACTCTAGCACTGGCAGGCATCCCGAGTGAATTGCCGCGCACTTGCCGCTGTCGCAGATAAAACATGCCATCACGAGGAACCCATCATGAAACTGCACTATCTGCTAc < 5:168073/137‑1 (MQ=255) ccTCGGGGTCGCAAAAAAGAAGGCGACTCTAGCACTGGCAGGCATCCCCAGTGAATTGCCGCGCACTTGCCGCTGTCGCAGATAAAACATGCCATCACGAGGACCCCATCATGAAACTTCACTATCTGCTACTcgcc > 5:173924/1‑137 (MQ=255) aaaaaaGAAGGCGACTCTAGCACTGGCAGGCATCCCGAGTGAATTGCCGCGCACTTGCCGCTGTCGCAGATAAAACATGCCATCACGAGGAACCCATCATGAAACTGCACTATCTGCTACTCGCCGCCCtgccttgc > 7:294931/1‑137 (MQ=255) | GTCGAGCTGCGCAGTCGGCTTGCCGCCACGCGCCGTTTCCATGGTCGCAGAAGCACAACCGGCCAGCATCAGCACCGCAGCCAACGGCAGAATCGCAC‑GCATGTTCCATCCTCGGGGTCGCAAAAAAGAAGGCGACTCTAGCACTGGCAGGCATCCCGAGTGAATTGCCGCGCACTTGCCGCTGTCGCAGATAAAACATGCCATCACGAGGAACCCATCATGAAACTGCACTATCTGCTACTCGCCGCCCTGCCTTGC > ACB143/4424940‑4425197 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |