Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1435,383,7580CG46.7% 31.5 / 16.7 31intergenic (+528/‑330)CJ019_05064/CJ019_05067putative protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (0/16);  new base G (14/0);  total (15/16)
Fisher's exact test for biased strand distribution p-value = 6.88e-09
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

TCGGGCGGCTGCGGATTAAATCAGTAGACACGGCTAAGCATGTAGTACCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGT‑CTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAGTCT  >  ACB143/5383631‑5383888
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tCGGGCTGCTGCGGATTAAATCAGTAGACACGGCTAAGCATGTAGTACCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCTCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGT‑CTTTTTTtgt                                                                                                                           <  8:87238/137‑1 (MQ=255)
                     cAGTAGACACGGCTAAGCATGTAGTACCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGT‑GTTTTTTTTTGGGGGGGATTTTGGCGGTGCg                                                                                                      >  4:207541/1‑137 (MQ=255)
                        gaGACACGGCTAAGCATGTAGTACCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGT‑CTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGt                                                                                                   <  5:85280/136‑1 (MQ=255)
                           acacGGCTAAGCATGTAGTACCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGACGACGCCCTCGGGCGG‑GGTTTTTTTTGGCTGGAATTTTGGGGGTGCGGGTAGa                                                                                                >  2:245319/1‑137 (MQ=255)
                                  tAAGCATGTAGTACCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGT‑GTTTTTTTTTGGCGGGAATTTGGGCGCGGCGGGGCGAAAAAtag                                                                                         >  5:269114/1‑135 (MQ=255)
                                  tAAGCATGTAGTACCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGT‑GGGTTTTTTTGGCGGGAATTTTGGGGCGGCGGGTCGAAAAAtat                                                                                         >  2:323183/1‑135 (MQ=255)
                                       aTGTAGTACCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGT‑GGTTTTTTGTGGCTGGAATTTTGGCGCTGGGGGTCGAAAAATCGGAttt                                                                                    >  6:85280/1‑137 (MQ=255)
                                         ttagtaCCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGT‑CTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTaa                                                                                  <  6:197686/136‑1 (MQ=255)
                                           agtaCCGACAGCGGAGTACTGGCGGACGGGGGATCAAATCCCCCCGGCTCCACCAAATCCCACGAAGAAGACGCCCTCGGGCGT‑CTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAAcc                                                                                <  8:136284/137‑1 (MQ=255)
                                            gtaCCTACAGCGGAGTACTGGCGGACGGGGGATCAAATCCCACCGGCTCCACCAAATCCAAAGAAGAAGACGACCTCGGGCGT‑GGTTTTTTTTGCCTGGAAATTTGGGGCTTCGGGTCGAAAAAAACGATTTAAcac                                                                               >  8:311045/1‑135 (MQ=255)
                                            gtaCCGACAGCTGAGTACTGGCGGACGGCGGTTCAAATCCAACCGGCTCCACCAAAAACAAAGAAGAAGACGCCCTCGGGCGTGATTTTTTTTTGCGGGGAGTTT‑GGAGCTGGGGGTGCAAAAAACAGAATTAAcca                                                                               >  6:62718/1‑136 (MQ=255)
                                            gtaCCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGT‑GGTTTTTTTTTGGGGGGATTTGGGGGGGGCGGGGGGAAAAAACGGtttttaacc                                                                               >  4:170527/1‑132 (MQ=255)
                                            gtaCCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGT‑CTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAAccc                                                                               <  2:254190/137‑1 (MQ=255)
                                              aCCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGT‑GGTTTTTTGTGCCTGGAATTTTGGCGGGGGGGGTGGAAAAATAGGTTTTaaaacca                                                                             >  7:105067/1‑132 (MQ=255)
                                                     gCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGT‑GTTTTTTTTTTCGTGGAATTTTGTGGCGGCGGGGGGAAAAAAACGATTTTAACCCTTTGtttt                                                                      >  6:127192/1‑135 (MQ=255)
                                                        gAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGT‑CTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAgg                                                                   <  4:295224/137‑1 (MQ=255)
                                                            aCTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGT‑GGTTTTTTTTGGCGGGGTTTTTGTGGCGTGGGGGCGAAAAAACGGCTTTATCCCCATTCTTTTattgggg                                                               >  8:31121/1‑131 (MQ=255)
                                                               ggCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGT‑CTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTc                                                            <  3:26707/137‑1 (MQ=255)
                                                               ggCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGT‑CTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTc                                                            <  1:297823/137‑1 (MQ=255)
                                                               ggCGGACGGGGGCTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGT‑CTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTc                                                            <  3:252877/137‑1 (MQ=255)
                                                                     cGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCACAGAAGAAGACGCCCTCGGGCGT‑CTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAg                                                      <  6:258197/137‑1 (MQ=255)
                                                                               aaTCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGT‑CTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATActgcctg                                            <  6:151856/137‑1 (MQ=255)
                                                                                                       aaaGAAGAAGACGCCCTCGGGCGT‑GGTTTTTTTTGGGTGGTATTTGGGGGCGGCGGGGGGGAAAAACGGGTTTTAACCCGTTGTTGTTGGGGGGTTGCCAAAGGTACTGCCTGCGCCGGTTTTAAGTTggggggatt                    >  4:115568/1‑132 (MQ=255)
                                                                                                       aaaGAAGAAGACGCCCTCGGGCGG‑GGTTTTTTTTTGCTGGAATTTTGGGGCGGCGGGGGGAAAAAACGGGTTTAACCCCGGTGTTGGTGGGGGGGTCCCAAACATACTGCCTGCGCCGGGTTTGCGGTGGGGGCAtt                    >  8:202513/1‑137 (MQ=255)
                                                                                                          caagaagaCGCCCTCGGGCGT‑CTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTGTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCAttttt                 <  4:186485/136‑1 (MQ=255)
                                                                                                               agaCGCCCTCGGGCGT‑CTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAgg            <  2:24917/137‑1 (MQ=255)
                                                                                                                 aCGCCCTCGGGCGT‑GTTTTTTTTTGGCTGGAATTTGGGCGCTGCGGGTCGAAAAATAGGATTTTACCCCTTTGTTGTAGGGCGTTTGCCCAAGATAATGCCAGCGCCTGTTTTACGTTGGGGTCATTTTTTGAGGGt          >  6:157535/1‑137 (MQ=255)
                                                                                                                   gCCCTCGGGCGT‑GGTTTTTTGTGGCGGGAATTTTGGCGCTGCGGGGGGAAAAATCGGATTTAACCCCTTTATTTTAGGGGGTTTCCCAAAGATAATGCCTGCGCCTGTTTTCAGTTGCGGTCATTTTTTAAGGGttt        >  4:237276/1‑137 (MQ=255)
                                                                                                                        cGGGCGT‑CTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAGtc   <  3:207541/137‑1 (MQ=255)
                                                                                                                         gggCGT‑CTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAGtct  <  2:260299/137‑1 (MQ=255)
                                                                                                                         gggCGT‑CTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAGtct  <  6:37514/137‑1 (MQ=255)
                                                                                                                                |                                                                                                                                  
TCGGGCGGCTGCGGATTAAATCAGTAGACACGGCTAAGCATGTAGTACCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGT‑CTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAGTCT  >  ACB143/5383631‑5383888

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑