Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1435,829,0750AC32.3% 94.3 / 12.7 62V88G (GTA→GGA) ftsYSignal recognition particle receptor FtsY
Reads supporting (aligned to +/- strand):  ref base A (35/7);  new base C (0/20);  total (35/27)
Fisher's exact test for biased strand distribution p-value = 9.65e-11
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

GCCTGCAAGGGCATGGACGCAACCGGCTGCGGGGCAGGTGCCACCAGCGGCTGGGATGCGACCGGCTCTGGCTCAGGCGCCTGCAACGGCTGCGACGCAACCGGTTCAGGGACGGGGGCCTCGACTACCGGAGGCCGGGGGGGTGCGGCGACCGGCTGCGGCTCAGCCAGCTGTGGGGCCGCAACGGCCTGAACGGGTTCGGGGGCCTGTGGCGCTTCGGCGGCAGATCCCTCGACCGGAGCGGCTGCAGACTGCTCGACA  >  ACB143/5828949‑5829209
                                                                                                                              |                                                                                                                                      
gccTGCAAGGGCATGGACGCAACCGGCTGCGGGGCAGGTGCCACCAGCGGCTGGGATGCGACCGGCTCTGGCTCAGGCGCCTGCAACGGCTGCGACGCAACCGGTTCAGGGACGGGGGCCTCGACTACCGGAGGCCg                                                                                                                              >  1:61681/1‑137 (MQ=255)
gccTGCAAGGGCATGGACGCAACCGGCTGCGGGGCAGGTGCCACCAGCGGCTGGGATGCGACCGGCTCTGGCTCAGGCGCCTGCAACGGCTGCGACGCAACCGGTTCAGGGACGGGGGCCTCGACTACCGGAGGCCg                                                                                                                              >  4:265335/1‑137 (MQ=255)
 ccTGCAAGGGCATGGACGCAACCGGCTGCGGGGCAGGTGCCACCAGCGGCTGGGATGCGACCGGCTCTGGCTCAGGCGCCTGCAACGGCTGCGACGCAACCGGTTCAGGGACGGGGGCCTCGACTACCGGAGGCCgg                                                                                                                             >  5:148224/1‑137 (MQ=255)
 ccTGCAAGGGCATGGACGCAACCGGCTGCGGGGCAGGTGCCACCAGCGGCTGGGATGCGACCGGCTCTGGCTCAGGCGCCTGCAACGGCTGCGACGCAACCGGTTCAGGGACGGGGGCCTCGACTACCGGAGGCCgg                                                                                                                             >  2:97250/1‑137 (MQ=255)
   tGCAAGGGCATGGACGCAACCGGCTGCGGGGCAGGTGCCACCAGCGGCTGGGATGCGACCGGCTCTGGCTCAGGCTCCTGCAACGGCTGCGACGCAACCGGTTCAGGGACGGGGGCCTCGACTACCGGAGGCCgggg                                                                                                                           >  2:20858/1‑137 (MQ=255)
   tGCAAGGGCATGGACGCAACCGGCTGCGGGGCAGGTGCCACCAGCGGCTGGGATGCGACCGGCTCTGGCTCAGGCGCCTGCAACGGCTGCGACGCAACCGGTTCAGGGACGGGGGCCTCGACTACCGGAGGCCgggg                                                                                                                           >  4:154232/1‑137 (MQ=255)
        gggCATGGACGCAACCGGCTGCGGGGAAGGTGCCACCAGCGGCTGGGATGCGACCGGCTCTGGCTCAGGCGCCTGCAACGGCTGCGACGCAACCGGTTCAGGGACTGTGGCCTCGACTACCGGAGGCCGGGGGGGTg                                                                                                                      >  7:258423/1‑137 (MQ=255)
                aCGCAACCGGCTGCGGGGCAGGTGCCACCAGCGGCTGGGATGCGACCGGCTCTGGCTCAGGCGCCTGCAACGGCTGCGACGCAACCGGTTCAGGGACGGGGGCCTCGACTACCGGAGGCCGGGGGGGTGCGGCGAcc                                                                                                              >  5:129789/1‑137 (MQ=255)
                aCGCAACCGGCTGCGGGGCAGGTGCCACCAGCGGCTGGGATGCGACCGGCTCTGGCTCAGGCGCCTGCAACGGCTGCGACGCAACCGGTTCAGGGACGGGGGCCTCGACTACCGGAGGCCGGGGGGGTGCGGCGAcc                                                                                                              >  6:230047/1‑137 (MQ=255)
                aCGCAACCGGCTGCGGGGCAGGTGCCACCAGCGGCTGGGATGCGACAGGCTATGTCTCAGGAGCCTGCAACGGCTGCGACGCAACAGGATCAGTGAAGGGTGCCTCGACTACCGGAGGCCGGGGGGGTGCGGCGAcc                                                                                                              >  3:295718/1‑137 (MQ=255)
                  gCAACCGGCTGCGGGGCAGGTGCCACCAGCGGCTGGGATGCGACCGGCTCTGGCTCAGGCGCCTGCAACGGCTGCGACGCAACCGGTTCAGGGACGGGGGCCTCGACTACCGGAGGCCGCGGGGGTGCGGCGACCgg                                                                                                            >  7:157916/1‑137 (MQ=255)
                      ccGGCTGGGGGGCAGGTGCCACCAGCGGCTGGGCTGCGCCCGGCTCGGGCACAGGCGCCGGCACCGGCGGCCACGCAACCGGTTCCGGGCCGGGGGCCTCGACTCCCGGAGGCCGGGGGGGTGCGGCGACCGGCTGc                                                                                                        <  3:195465/137‑1 (MQ=255)
                       cGGCTGCGGGGCAGGTGCCACCAGCGGCTGGGATGCGACCGGCTCTGGCTCAGGCGCCTGCAACGGCTGCGACGCAACCGGTTCAGGGACGGGGGCCTCGACTACCGGAGGCCGGGGGGGTGCGGCGACCGGCTGCg                                                                                                       >  7:158440/1‑137 (MQ=255)
                       cGGCTGCGGGGCAGGTGCCACCAGCGGCTGGGATGCGACCGGCTCTGGCTCAGGCGCCTGCAACGGCTGCGACGCAACCGGTTCAGGGACGGGGGCCTCGACTACCGGAGGCCGGGGGGGTGCGGCGACCGGCTGCg                                                                                                       >  7:54423/1‑137 (MQ=255)
                       cGGCTGCGGGGCAGGTGCCACCAGCGGCTGGGATGCGACCGGCTCTGGCTCAGGCGCCTGCAACGGCTGCGACGCAACCGGTTCAGGGACGGGGGCCTCGACTACCGGAGGCCGGGGGGGTGCGGCGACCGGCTGCg                                                                                                       >  3:34026/1‑137 (MQ=255)
                       cGGCTGCGGGGCAGGTGCCACCAGCGGCTGGGATGCGACCGGCTCTGGCTCAGGCGCCTGCAACGGCTGCGACGCAACCGGTTCAGGGACGGGGGCCTCGACTACCGGAGGCCGGGGGGGTGCGGCGACCGGCTGCg                                                                                                       >  6:249771/1‑137 (MQ=255)
                           tgatgggCAGGTGACACCAGGGGCTGGGATGCTACCGTCTCTGGCTCCCGCTCCTGCAACGGCTGCGACGCCACCGGTTAAGGGACGGGGGCCTCGACTACCTGAGTCCTGTGGGGTGCGGCTACCGGCTGCGGCTc                                                                                                   <  6:114550/133‑1 (MQ=255)
                            gCGGGGCTGGTGCCACCCGCGGCTGGGAGGCGCCCGGCGCTGGCCCAGGCGCCGGCAACGGCGGCGACGCACCCGGGTCCGGGCCGGGGGCCTCGACTCCCGGCGGCCGGGGGGGGGCGGCGACCGGCTGCGGCTca                                                                                                  <  4:164413/137‑1 (MQ=255)
                            gCGGGGCAGGTGCCACCAGCGGCGGGGTGGCGACCGGCTCTGGCTCAGGCGCCTCCCCGGGCTGCGACGCAAACGGTTGAGCGCCGGGGGCCTCCACTACCGGAGGCCGGGGGGGTGCGGCGACCGGCTGCGGCTca                                                                                                  <  8:315512/137‑1 (MQ=255)
                             cGGGGCAGGTGCCACCAGCGGCTGGGATGCGACCGGCTCTGGCTCAGGCGCCTGCAACGGCTGCGACGCAACCGGTTCAGGGACGGGGGCCTCGACTACCGGAGGCCGGGGGGGTGCGGCGACCGGCTGCGGCTcag                                                                                                 >  4:76355/1‑137 (MQ=255)
                               gggCAGGTGCCACCAGCGGCTGGGATGCGACCGGCTCTGGCTCAGGCGCCTGCAACGGCTGCGACGCACCCGGTGCAGGGACGGGGGCCTCGACTACCGGAGGCCGGGGGGGTGCGGCGCCCGGCTGCGGCTcagcc                                                                                               >  8:287149/1‑137 (MQ=255)
                                      ggCCCCCAGCGGCGGGGATGCGCCCGGCGCGGGCGCCGGCGCCGGCACCGGCGGCGACGCACCCGGGGCCGGGCCGGGGGCCTCGCCTCCCGGCGGCCGGGGGGGGGCGGCGACCGGCTGCGGCTCAGCCAGCtgtg                                                                                        <  2:55825/136‑1 (MQ=255)
                                      ggCCACCCGCGGCTGGGCTGCGACGGGCTCGGGCTCCGGCGCCTGCACCGGCGGCGACGCACCGGGGGCAGGGCCGGGGGCCGCGCCTCCCGGCGGCCGGGGGGGGGCGGCGACCGGCTGCGGCTCAGCCAGCtgtg                                                                                        <  2:274580/136‑1 (MQ=255)
                                      ggCCACCCGCGGCTGGGCCGCGCCCGGCTCGGGCGCCGGCGCCTGCAACGGCTCCGACGCAACCGGGCCAGGGCCGGGGGCCCCGCCTCCCGGCGGCCGGGGGGGGGCGGCGACCGGCTGCGGCTCAGCCAGCtgtg                                                                                        <  4:78868/136‑1 (MQ=255)
                                           ccaGCGGCTGGGCGGCGACCGGCTCTGGCACTGGCGCCTGCAACGCCTGCGACGCCACCGGCTCCGGGACGGGGGCCGCGACTCCCGGAGGCCGGGGGGGTGCGGCGACCGGCTGCGGCTCAGCCAGCTGTGGGGcc                                                                                   <  4:317228/137‑1 (MQ=255)
                                               cGGCTGGGATGCGACCGGCTCTGGCTCAGGCGCCTGCAACGGCTGCGACGCAACCGGTTCAGGGACGGGGGCCTCGACTACAGGAGGCCGGGGGGGTGCGGCGACCGGCTGCGGCTCAGCCAGCTGTGCGGCCGCac                                                                               >  2:318714/1‑136 (MQ=255)
                                                  cTGGGATGCGACCGGCTCTGGCTCAGGCGCCTGCAACGGCTGCGACGCAACCGGTTCAGGGACGGGGGCCTCGACTACCGGAGGCCGGGGGGGTGCGGCGACCGGCTGCGGCTCAGCCAGCTGTGGGGCCGCAACgg                                                                            >  2:222446/1‑137 (MQ=255)
                                                             ccGGCTCTGGCGCCGGCGCCGGCACCGGCTGCGCCGCAACGGGTTCAGGGACGGGGGCCTCGACTCCCGGCGGCCGGGGGGGTGCGGCGACCGGCTGCGGCTCAGCCAGCTGTGGGGCCGCAACGGCCTGAACGGGt                                                                 <  3:162065/137‑1 (MQ=255)
                                                               ggCGCTGGCTCAGCCGCCTGCCACGGCTGCGACGCAACCGGTGCAGGGACGGGGGCCGCGACTCCCGGAGGCCGGGGGGGGGCGGCGACCGGCTGCGGCTCAGCCAGCTGTGGGGCCGCAACGGCCTGAACGGGTTc                                                               <  2:178621/137‑1 (MQ=255)
                                                                         cAGGCCCCGGCACGGGCGGCGACGCCACCGGTTCCGGGCCGGGCGCCGCGACTCCCGGAGGCCGGGGGGGGGCGGCGACCGGCTGCGGCTCAGCCAGCTGTGGGGCCGCAACGGCCTGAACGGGTTCGGGGGCCtgt                                                     <  5:201302/137‑1 (MQ=255)
                                                                             cgcCGGCCACGGCGGCGACGCCCCCGGGTCCGGGCCGGGGGCCGCGACTCCCGGCGGCCGGGGGGGGGCGGCGACCGGCTGCGGCTCAGCCAGCTGTGGGGCCGCAACGGCCTGAACGGGTTCGGGGGCCTGTGgcg                                                 <  6:3399/137‑1 (MQ=255)
                                                                             cgcCGGCAACGGCGGCCCCCCCACCGGTGCCGGGACGGGGGCCTCGACTCCCGGCGGCCGGGGGGGGGCGGCGACCGGCTGCGGCTCAGCCAGCTGTGGGGCCGCAACGGCCTGAACGGGTTCGGGGGCCTGTGgcg                                                 <  6:95820/137‑1 (MQ=255)
                                                                              gcCTGCAACGGCTGCGACGCAACCGGTTCAGGGACGGGGGCCTCGACTACCGGAGGCCGGGGGGGTGCGGCGACCGGCTGCGGCTCAGCCAGCTGTGGGGCCGCAACGGCCTGAACGGGTTCGGGGGCCTGTGgcgc                                                >  8:189209/1‑137 (MQ=255)
                                                                              gcCTGCAACGGCTGCGACGCAACCGGTTCAGGGACGGGGGCCTCGACTACCGGAGGCCGGGGGGGTGCGGCGACCGGCTGCGGCTCAGCCAGCTGTGGGGCCGCAACGGCCTGAACGGGTTCGGGGGCCTGTGgcgc                                                >  7:199940/1‑137 (MQ=255)
                                                                              gcCTGCAACGGCTGCGACGCAACCGGTTCAGGGACGGGGGCCTCGACTACCGGAGGCCGGGGGGGTGCGGCGACCGGCTGCGGCTCAGCCAGCTGTGGGGCCGCAACGGCCTGAACGGGTTCGGGGGCCTGTGgcgc                                                >  6:168297/1‑137 (MQ=255)
                                                                              gcCTGCAACGGCTGCGACGCAACCGGTTCAGGGACGGGGGCCTCGACTACCGGAGGCCGGGGGGGTGCGGCGACCGGCTGCGGCTCAGCCAGCTGTGGGGCCGCAACGGCCTGAACGGGTTCGGGGGCCTGTGgcgc                                                >  7:104938/1‑137 (MQ=255)
                                                                                   cAACGGCTGCGACGCAACCGGTTCAGGGACGGGGGCCTCGACTACCGGAGGCCGGGGGGGTGCGGCGACCGGCTGCGGCTCAGCCAGCTGTGGGGCCGCAACGGCCTGAACGGGTTCGGGGGCCTGTGGCGCTTcgg                                           >  6:47492/1‑137 (MQ=255)
                                                                                        gCTGCGACGCACCCGGGTCCGGGCCGGGGGCCGCGACACCCGGCGGCCGGGGGGGGGCGGCGACCGGCTGCGGCTCAGCCAGCTGTGGGGCCGCAACGGCCTGAACGGGTTCGGGGGCCTGTGGCGCTTCGGCGGCa                                      <  3:97355/137‑1 (MQ=255)
                                                                                         cTGCGACGCAACCGGTTCAGGGACGGGGGCCTCGACTACCGGAGGCCGGGGGGGTGCAGCGACCGGCTGCGGCTCAGCCAGCTGTGGGGCCGCAACGGCCTGAACGGGTTCGGGGGCCTGTGGCGCTTCGGCGGCAg                                     >  1:159289/1‑137 (MQ=255)
                                                                                                     gggTGCAGGGACGGGGGCCTCGCCTCCCGGAGGCCGGGGGGGGGCGGCGACCGGCTGCGGCTCAGCCAGCTGTGGGGCCGCAACGGCCTGAACGGGTTCGGGGGCCTGTGGCTCTTCTTCTTCAGATCCCTCGACCg                         <  1:24981/136‑1 (MQ=255)
                                                                                                     cGGTTCCGGGACGGGCGCCGCGACTACCGGAGGCCGGGGGGGTGCGGCGACCGGCTGCGGCTCAGCCAGCTGTGGGGCCGCAACGGCCTGAACGGGTTCGGGGGCCTGTGGCGCTTCGGCGGCAGATCCCTCGACCg                         <  7:68570/137‑1 (MQ=255)
                                                                                                     cGGGTCAGGGCCGGGGGCCGCGACTACCGGAGGCCGGGGGGGGGCGGCGACCGGCTGCGGCTCAGCCAGCTGTGGGGCCGCAACGGCCTGAACGGGTTCGGGGGCCTGTGGCGCTTCGGCGGCAGATCCCTCGACCg                         <  6:308462/137‑1 (MQ=255)
                                                                                                     cGGGTCAGGGACGGGGGCCTCGACACCCGGAGGCCGGGGGGGCGCGGCGACCGGCTGCGGCTCAGCCAGCTGTGGGGCCGCAACGGCCTGAACGGGTTCGGGGGCCTGTGGCGCTTCGGCGGCAGATCCCTCGACCg                         <  6:95773/137‑1 (MQ=255)
                                                                                                      ggTTCAGGGACGGGGGCCTCGACAACCGGAGGCCGGGGGGGTGCGGCGACCGGCTGCGGCTCAGCCAGCTGTGGGGCCGCAACGGCCTGAACGGGTTCGGGGGCCTGTGGCGCTTCGGCGGCAGATCCCTCGACCgg                        <  6:313689/137‑1 (MQ=255)
                                                                                                       gTTCAGGGACGGGGGCCTCGACTACCGGAGGCCGGGGGGGTGCGGCGACCGGCTGCGGCTCAGCCAGCTGTGGGGCCGCAACGGCCTGAACGGGTTCGGGGGCCTGTGGCGCTTCGGCGGCAGATCCCTCGCCCggc                       >  2:63452/1‑136 (MQ=255)
                                                                                                       gTTCAGGGACGGGGGCCTCGACTACCGGAGGCCGGGGGGGTGCGGCGACCGGCTGCGGCTCAGCCAGCTGTGGGGCCGCAACGGCCTGAACGGGTTCGGGGGCCTGTGGCGCTTCGGCGGCAGATCCCTAGACCGGa                       >  1:249910/1‑137 (MQ=255)
                                                                                                           cgggACGGGGGCCGCGACTCCCGGAGGCCGGGGGGGTGCGGCGACCGGCTGCGGCTCAGCCAGCTGTGGGGCCGCAACGGCCTGAACGGGTTCGGGGGCCTGTGGCGCTTCGGCGGCAGATCCCTCGACCGGAGCgg                   <  2:31801/136‑1 (MQ=255)
                                                                                                           aGGGACGGGGGCCTCGACTACCGGAGGCCGGGGGGGTGCGGCGACCGGCTGCGGCTCAGCCAGCTGTGGGGCCGCAACGGCCTGAACGGGTTCGGGGGCCTGTGGCGCTTCGGCGGCAGATCCCTCGACCGGAGCgg                   >  4:102860/1‑137 (MQ=255)
                                                                                                             ggccGGGGCCCCCGCCTCCCCGCGGCCGGGGGGGGGCGGCGACCGGCTGCGGCTCAGCCAGCTGTGGGGCCGCAACGGCCTGAACGGGTTCGGGGGCCTGTGGCGCTTCGGCGGCAGATCCCTCGACCGGAGCGGCt                 <  5:322733/134‑1 (MQ=255)
                                                                                                             ggccGGGCGCCTCGACTCCCGGCGGCCGGGGGGGGGCGGCGACCGGCTGCGGCGCCGCCAGCTGTGGGGCCGCAACGGCCTGAACGGGTTCGGGGGCCTGTGGCGCTTCGGCGGCAGATCCCTCGACCGGAGCGGCt                 <  6:273996/134‑1 (MQ=255)
                                                                                                             gccgggggcCCGCGACTCCCGGAGGCCGGGGGGGGGCGGCGACCGGCTGCGGCTCAGCCAGCTGGGGGGCCGCAACGGCCTGAACGGGTGCGGGGGCCTGTGGCGCTTCGGCGGCAGATCCCTCGACCGGAGCGGCt                 <  7:76688/133‑1 (MQ=255)
                                                                                                               ccGGGGGCCTCGACTACCGGAGGCCGGGGGGGTGCGGCGACCGGCTGCGGCTCAGCCAGCTGTGGGGCCGCAACGGCCTGAACGGGTTCGGGGGCCTGTGGCGCTTCGGCGGCAGATCCCTCGACCGGAGCGGCTGc               <  7:299888/136‑1 (MQ=255)
                                                                                                               ccGGGGGCCTCCACGCCCGGAGGCCGGGGGGGTGCGGCGACCGGCTGCGGCTCAGCCAGCTGTGGGGCCGCAACGGCCTGAACGGGTTCGGGGGCCTGTGGCGCTTCGGCGGCAGATCCCTCGACCGGAGCGGCTGc               <  2:175846/136‑1 (MQ=255)
                                                                                                               ccGGGGGCCCCCCCTCCCGCCGGCCGGGGGGGGGCGGCGACCGGCTGCGGCTCAGCCAGCTGTGGGGCCGCAACGGCCTGAACGGGTTCGGGGGCCTGTGGCGCTTCGGCGGCAGATCCCTCGACCGGAGCGGCTGc               <  4:209993/136‑1 (MQ=255)
                                                                                                               ccGGGCGCCTCGCCTACCGGCGGCCGGGGGGGGGCGGCGACCGGCTGCGGCTCAGCCAGCTGTGGGGCCGCAACGGCCTGAACGGGTTCGGGGGCCTGTGGCGCTTCGGCGGCAGATCCCTCGACCGGAGCGGCTGc               <  8:11753/136‑1 (MQ=255)
                                                                                                               aCGGGGGCCTCGACTACCGGAGGCCGGGGGGGTGCGGCGACCGGCTGCGGCTCAGCCAGCTGTGGGGCCGCAACGGCCTGAACGGGTTCGGGGGCCTGTGGCGCTTCGGCGGCAGATCCCTCGACCGGAGCGGCTGc               >  7:153572/1‑137 (MQ=255)
                                                                                                                     gCCTCGACTACCGGAGGCCGGGGGGGTGCGGCGACCGGCTGCGGCTCAGCCAGCTGTGGGGCCGCAACGGCCTGAACGGGTTCGGGGGCCTGTGGCGCTTCGGCGGCAGATCCCTCGACCGGAGCGGCTGCAGACTg         >  4:308833/1‑137 (MQ=255)
                                                                                                                     gCCTCGACTACCGGAGGCCGGGGGGGTGCGGCGACCGGCTGCGGCTCAGCCAGCTGTGGGGCCGCAACGGCCTGAACGGGTTCGGGGGCCTGTGGCGCTTCGGCGGCAGATCCCTCGACCGGAGCGGCTGCAGACTg         >  7:155989/1‑137 (MQ=255)
                                                                                                                     gCCTCGACTACCGGAGGCCGGGGGGGTGCGGCGACCGGCTGCGGCTCAGCCAGCTGTGGGGCCGCAACGGCCTGAACGGGTTCGGGGGCCTGTGGCGCTTCGGCGGCAGATCCCTCGACCGGAGCGGCTGCAGACTg         >  5:135836/1‑137 (MQ=255)
                                                                                                                          gACTACCGGAGGCCGGGGGGGTGCGGCGACCGGCTGCGGCTCAGCCAGCTGTGGGGCCGCAACGGCCTGAACGGGTTCGGGGGCCTGTGGCGCTTCGGCGGCAGATCCCTCGACCGGAGCGGCTGCAGACTGCTCgc    >  6:270810/1‑136 (MQ=255)
                                                                                                                           aCTACCGGAGGCCGGGGGGGTGCGGCGACCGGCTGCGGCTCAGCCAGCTGTGGGGCCGCAACGGCCTGAACGGGTTCGGGGGCCTGTGGCGCTTCGGCGGCAGATCCCTCGACCGGAGCGGCTGCAGACTGCTCGAc   >  6:186608/1‑137 (MQ=255)
                                                                                                                            cTACCGGAGGCCGGGGGGGTGCGGCGACCGGCTGCGGCTCAGCCAGCTGTGGGGCCGCAACGGCCTGAACGGGTTCGGGGGCCTGTGGCGCTTCGGCGGCAGATCCCTCGACCGGAGCGGCTGCAGACTGCTCGACa  >  6:226278/1‑137 (MQ=255)
                                                                                                                              |                                                                                                                                      
GCCTGCAAGGGCATGGACGCAACCGGCTGCGGGGCAGGTGCCACCAGCGGCTGGGATGCGACCGGCTCTGGCTCAGGCGCCTGCAACGGCTGCGACGCAACCGGTTCAGGGACGGGGGCCTCGACTACCGGAGGCCGGGGGGGTGCGGCGACCGGCTGCGGCTCAGCCAGCTGTGGGGCCGCAACGGCCTGAACGGGTTCGGGGGCCTGTGGCGCTTCGGCGGCAGATCCCTCGACCGGAGCGGCTGCAGACTGCTCGACA  >  ACB143/5828949‑5829209

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑