| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | ACB143 | 6,087,126 | 0 | A | C | 53.3% | 1.4 / 11.8 | 15 | intergenic (+7/+25) | spuD_6/gabR_2 | Putrescine‑binding periplasmic protein SpuD/HTH‑type transcriptional regulatory proteinGabR |
| Reads supporting (aligned to +/- strand): ref base A (3/4); new base C (0/8); total (3/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.69e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.48e-02 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TTCGCAACAACCCCAACCTGTATCCGACGGCAGAGGCGATGGCCAAGCTGTATACCCTCAAGCCTTTGACGCGAAATGCCGAACGGGCCAGGACCCGCGCCTGGACGCGGATAAAGTCCGGTACGTGATGTTGAACCGGGGGCTGCTTTGCAGCCCCCTTCATTTTCTCCAGACTTTGCGTAATTTCATCAAGGCTTCGGCTATCTCGCCCTCCGGCACCGCCGCGAAGCCCAGCACCAGCCCC > ACB143/6086992‑6087235 | ttCGCAACAACCCCAACCTGTATCCGACGGCAGAGGCGATGGCCAAGCTGTATACCCTCAAGCCTTTGACGCGAAATGCCGAACGGGCCAGGACCCGCGCCTGGACGCGGATAAAGTCCGGTACGTGATGTTGAAcc > 8:24847/1‑137 (MQ=255) cGCAACAACCCCAACCTGTATCCGACGGCAGAGGCGATGGCCAAGCTGTATACCCTCAAGCCTTTGACGCGAAATGCCGAACGGGCCAGGACCCGCGCCTGGACGCGGATAAAGTCCGGTACGTGATGTTGAACCgg < 1:200615/137‑1 (MQ=255) cGCAACAACCCCAACCTGTATCCGACGGCAGAGGCGATGGCCAAGCTGTATACCCTCAAGCCTTTGACGCGAAATGCCGAACGGGCCAGGACCCGCGCCTGGACGCGGATAAAGTCCGGTACGTGATGTTGAACCgg < 3:54198/137‑1 (MQ=255) agGCGATGGCCAAGCTGTATACCCTCAAGCCTTTGACGCGAAATGCCGAACGGGCCAGGACCCGCGCCTGGACGCGGATAAAGTCCGGTACGTGATGTTGAACCGGGGGCGGCGTTGGCGCCCCCCTCCttttttgc > 8:69334/1‑133 (MQ=255) aCGCTAAATGCTGAAGGGGCCCGCCCCCGCGGCTGGACGCTTTAAACGCCCGGTCCGTGAGGTTTACGCGGGGGCTGCTTTGCAGCCCCCTTCATTTTCTCCTGACTTTGCGTAATTTCATCAAGGCTTCGGCTAtc < 2:181393/137‑1 (MQ=255) ctaccGGGCCAGCCCCCGCGCCGGGACGCGTATAACGACGGGAACGTGATGTTCACCGGGGGGCGGCTTGCCAGCCCCCTTCATTTTCTCCAGACTTTGCGTAATTTCATCAAGGCTTCGGCTATCTCGCCCTCCgg < 5:168101/133‑1 (MQ=255) gcccccgACCCGCGCCGGGCCGCGTAAACAGGCCGTGTCGGCATTTTCACCCGGGGGCGGCTTTCCACCCCCCTTCATTTTCTCCAGACTTTGCGTAATTTCATCAAGGCTTCGGCTATCTCGCCCTCCGGCAccgc < 2:174761/131‑1 (MQ=255) gCCAGCACCCGCGCCGGGTCGCGTATAAAGGCCGTAACGTTATGTTCACCCGGGGGTTGCTTGCCACCCCCCTTCATTTTCTCCAGACTTTGCGTAATTTCATCAAGGCTTCGGCTATCTCGCCCTCCGGCAccgcc < 5:112608/137‑1 (MQ=255) ccaaGGCCCCGCGCCTCGCCTCGTATAACGCCAGGTACTTAATGTGCCACCGGGGGCTGCTTTCACCCCCCCTTCATTTTCTCCAGACTTTGCGTAATTTCATCAAGGCGTCGGCTATCTCGCCCTCCGGCAccgcc < 4:202739/134‑1 (MQ=255) cccccGCGCCGGCACGCGTATAAAGCGCGTTCGGTGATTTTGACCGGGGGGCTGCTTTGAAGCCCCCTTCATTTTCTCCAGACTTTGCGTAATTTCATCAAGGCTTCGGCTATCTCGCCCTCCGGCACCGCCGCGaa < 8:160892/135‑1 (MQ=255) ccccGCGCGCGGCCGCGTAAAAAGGCCGTTAGGTGTTGTTCACCGGGGGGCTGCTTTCCCCCCCCCTTCATTTTCTCCAGACTTTGCGTAATTTCATCAAGGCTTCGGCTATCTCGCCCTCCGGCACCGCCGCGAAg < 8:10880/136‑1 (MQ=255) gcgcCTGGCCGCGTAAAAACTCCGGTACGTCTTGTTGAACCGGGGGCTGCTTTCCAGCCCCCTTCATTTTCTCCAGACTTTGCGTAATTTCATCAAGGCTTCGGCTATCTCGCCCTCCGGCACCGCCGCGAAGCCCa < 2:265350/137‑1 (MQ=255) ccTGGACGCGGATAAAGTCCGGTACGTGATGTTGAACCGGGGGCTGCTTTGCAACCCCCTTCATTTTCTCCAGACTTTGCGTAATTTCATCAAGGCTTCGGCTATCTCGCCCTCCGGCACCGCCGCGACGCCCAGcc > 5:335293/1‑136 (MQ=255) gggATAAAGTCGGGTAGGTGATTTTGACCCGGGGGCTGCTTTGCAGCCCCCTTCATTTTCTCCAGACTTTGCGTAATTTCATCAAGGCTTCGGCTATCTCGCCCTCCGGCACCGCCGCGAAGCCCAGCACCAGcccc < 8:123595/136‑1 (MQ=255) cgcaTAACGGCCGTTAGGTTATGTTCACCCGGGGGCGGGTTGCCCGCCCCCTTCATTTTCTCCAGACTTTGCGTAATTTCATCAAGGCTTCGGCTATCTCGCCCTCCGGCACCGCCGCGAAGCCCAGCACCAGcccc < 7:24847/134‑1 (MQ=255) | TTCGCAACAACCCCAACCTGTATCCGACGGCAGAGGCGATGGCCAAGCTGTATACCCTCAAGCCTTTGACGCGAAATGCCGAACGGGCCAGGACCCGCGCCTGGACGCGGATAAAGTCCGGTACGTGATGTTGAACCGGGGGCTGCTTTGCAGCCCCCTTCATTTTCTCCAGACTTTGCGTAATTTCATCAAGGCTTCGGCTATCTCGCCCTCCGGCACCGCCGCGAAGCCCAGCACCAGCCCC > ACB143/6086992‑6087235 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |