Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	ACB143	5,760,598	(A)6→5	coding (781/834 nt)	CJ019_05400 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	ACB143	5,760,593	0	A	.	98.0%	229.5 / ‑2.1	51	coding (776/834 nt)	CJ019_05400	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base . (37/12);  minor base C (0/1);  total (37/14)
Fisher's exact test for biased strand distribution p-value = 2.60e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AGAAGCTGCAAGCGCGCGAACAGGACCAGGCCGACCTGGCCAAGGTCTTGAAGACCATCGAGGAAACCCTCGCCCGCCAGGCCCGTG‑AAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGGAAAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAACCGCCGAAAAAGGCCCGTACCACCCTCGGGCCGATGGTTTCCAGCGAT  >  ACB143/5760458‑5760718                                                                                                                                         |                                                                                                                              aGAAGCTGCAAGCGCGCGAACAGGACCAGGCCGACCTGGCCAAGTTCTTGAAGACCATCGAGGAAACCCTCGCCCGCCTGGCCCGTG‑AAGCTGAAGAGGCACGCAAGAAGGCCGTGCTGGCGCAGCAGGAAGCGGa                                                                                                                               <  2:101947/136‑1 (MQ=255)         cAAGCGCGCGAACAGGACCAGGCCGACCTGGCCAAGGGCTTGAAGACCATCGAGGAAACCCTCGCCCGCCAGGCCCGTG‑AAGCTGAAGAGGCACGCAAGAAGGCCGTGCTGGCGCAGCAGGAAGCGGaaaaacgc                                                                                                                        <  4:233232/135‑4 (MQ=255)                  gAACAGGACCAGGCCGACCTGGCCAAGGTCTTGAAGACCATCGAGGAAACCCTCGCCCGCCAGGCCCGTG‑AAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGG‑AAAAACGCCGCcagcag                                                                                                              >  7:10883/1‑135 (MQ=255)                    aCAGGACCAGGCCGACCTGGCCAAGGTCTTGAAGACCATCGAGGAAACCCTCGCCCGCCAGGCCCGTG‑AAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAgg                                                                                                             >  7:139168/1‑134 (MQ=255)                     cAGGACCCGGACGAGCTGGCCAAGGTCTCGAAGACCATCGAGGAACCCCGCGCCCGCCCGGCCCGTG‑ACGCGGAACAGGCACGCAGGATGGCCCTGCTGGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAg                                                                                                          <  6:335006/136‑1 (MQ=255)                                cGACCTGGCCAAGGTCTTGAAGACCATCGAGGAAACCCTCGCCCGCCAGGCCCGTG‑AAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGgccgcc                                                                                               >  3:224638/1‑136 (MQ=255)                                 gACCTGGCCAAGGTCTTGAAGACCATCGAGGAAACCCTCGCCCGCCAGGCCCGTG‑AAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGgccgccg                                                                                              >  3:92954/1‑136 (MQ=255)                                     tGGCCAAGGTCTTGAAGACCATCGAGGAAACCCTCGCCCGCCAGGCCCGTG‑AAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGCAGCGCTGGCcgccgccgc                                                                                          >  1:148035/1‑136 (MQ=255)                                     tGGCCAAGGTCTTGAAGACCATCGAGGAAACCCTCGCCCGCCAGGCCCGTG‑AAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCcgccgccgc                                                                                          >  3:312055/1‑136 (MQ=255)                                       gCCATGGTCTTTAACACCATCGCGGAACCCCGCGCCCGCCA‑GCCCGTGCACGCTCAAGCGGCACCCAAGAAGGCCCGGCTGGCGCAGCAGGACGC‑GCAAAACCGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTg                                                                                        <  2:316342/136‑1 (MQ=255)                                       gCCAAGGTCTTGAAGACCATCGAGGAAACCCTCGCCCGCCAGGCCCGTG‑AAGCTGAAGCGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTg                                                                                        <  2:284786/136‑1 (MQ=255)                                          aaGGTCTTGAAGACCATCGAGGAAACCCTCGCCCGCCAGGCCCGTG‑AAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGccc                                                                                     >  5:159318/1‑136 (MQ=255)                                          aaGGTCTTGAAGACCATCGAGGAAACCCTCGCCCGCCAGGCCCGTG‑AAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGccc                                                                                     >  4:154497/1‑136 (MQ=255)                                           aGGTCTGGAAGCCCACCCCGGAACCCCTCCCCCGCCCGGCCCGGT‑ACGCTCACCAGGCACGCACCACGGCCCTGCGGGCGCAGCTGGACGCGG‑AAAACCGCCGCCAGCAGGAAGCGCGGGCCGCCGCCGCTGCCcg                                                                                    <  4:183695/136‑1 (MQ=255)                                            ggTCTTGAAGACCATCGAGGAAACCCTCGCCCGCCAGGCCCGTG‑AAGGTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCcgc                                                                                   <  8:35148/136‑1 (MQ=255)                                               cTTGAAGACCATCGAGGAAACCCTCGCCCGCCAGGCCCGTG‑AAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGAc                                                                                >  6:329721/1‑136 (MQ=255)                                               cTTGAAGACCATCGAGGAAACCCTCGCCCGCCAGGCCCGTG‑AAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGAc                                                                                >  8:99506/1‑136 (MQ=255)                                                    aggccATCGAGGAAACCCTCGCCCGCCAGGCCCGTG‑AAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGc                                                                           >  7:325105/4‑136 (MQ=255)                                                    aGACCATCGAGGAAACCCTCGCCCGCCAGGCCCGTG‑AAGCTGAAGAGGCACGCAAGCAGGCCCTGCTGGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGc                                                                           >  1:272185/1‑136 (MQ=255)                                                    aGACCATCGAGGAAACCCTCGCCCGCCAGGCCCGTG‑AAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGc                                                                           >  8:277438/1‑136 (MQ=255)                                                    aGACCATCGAGGAAACCCTCGCCCGCCAGGCCCGTG‑AAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGc                                                                           >  7:279160/1‑136 (MQ=255)                                                           cGAGGAAACCCTCGCCCGCCAGGCCCGTG‑AAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGG‑AAACACGCCGCCAGCAGGAAGCGCTGGCCCCCGCCGCTGCCCGCGACCGGGCCCGTgcg                                                                    >  8:257065/1‑134 (MQ=255)                                                           cGAGGAAACCCTCGCCCGCCAGGCCCGTG‑AAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTgcg                                                                    >  1:296374/1‑134 (MQ=255)                                                           cGAGGAAACCCTCGCCCGCCAGGCCCGTG‑AAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAg                                                                    >  4:116821/1‑136 (MQ=255)                                                           cGAGGAAACCCTCGCCCGCCAGGCCCGTG‑AAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCACCAGGAAGCGG‑AAAAACGCCGCCAGCAGGATGCGCTGGCCGCCGACGCTGCCCGCGACCGGGCCCGTGAg                                                                    >  7:17922/1‑136 (MQ=255)                                                           cGAGGAAACCCTCGCCCGCCAGGCCCGTG‑AAGCTGAAGAGGCACGCAAGAAGGACCTGCTGGCGCAGCAGGAAGCGG‑AAAAACGCCTCCAGCAGGAAGCGCTGGCCGACGCAGCTGCCAGCGACCGGGCCCGTGAg                                                                    >  6:49646/1‑136 (MQ=255)                                                            gAGGAACCCCTCGCCCGCCAGGCCCGTG‑AAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGc                                                                   <  5:269866/136‑1 (MQ=255)                                                            gAGCAAACCCTCGCCCGCCAGGCCCGGG‑AAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGTAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGc                                                                   <  5:99197/136‑1 (MQ=255)                                                             aGGAAACCCTCGCCCGCCAGGCCCGTG‑AAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGcc                                                                  >  4:311134/1‑136 (MQ=255)                                                             aGGAAACCCTCGCCCGCCAGGCCCGTG‑AAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGcc                                                                  >  6:131537/1‑136 (MQ=255)                                                                     cTCGCCCGCCAGGCCCGTG‑AAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGc                                                          >  6:115794/1‑136 (MQ=255)                                                                          cccccAGGCCCGTG‑AAGCTCAAGAGGCCCGCAAGACGGCCTTGCGGGCGCAGATGTACGC‑GTAAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTg                                                     <  4:327788/133‑1 (MQ=255)                                                                                   ccGTT‑AAGCTGAAGTGGCACGAAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAAccgccg                                            <  3:345761/136‑1 (MQ=255)                                                                                          aGCTGAAGAGGCACGCACGACGGCCCTGCTGGCGCAGCTGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAACCGCCGaaaaa                                       <  2:255616/135‑1 (MQ=255)                                                                                          aGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAACCGCCGAAAAAg                                      >  1:103535/1‑136 (MQ=255)                                                                                          aGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAACCGCCGAAAAAg                                      >  2:327634/1‑136 (MQ=255)                                                                                          aGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAACCGCCGAAAAAg                                      >  5:165666/1‑136 (MQ=255)                                                                                          aGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAACCGCCGAAAAAg                                      >  5:259665/1‑136 (MQ=255)                                                                                               aaGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGCGCCAGTGGAACCGCCGAAAAAGGCCCg                                 >  6:108068/1‑136 (MQ=255)                                                                                                     cACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAACCGCCGAAAAAGGCCCGTaccac                           <  8:279160/136‑1 (MQ=255)                                                                                                       cGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAACCGCCGAAAAAGGCCCGTACCAccc                         >  2:293923/1‑136 (MQ=255)                                                                                                        gCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAACCGCCGAAAAAGGCCCGTACCACCCt                        >  6:149099/1‑136 (MQ=255)                                                                                                         cAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAACCGCCGAAAAAGGCCCGTACCACCCTc                       >  5:207887/1‑136 (MQ=255)                                                                                                            gacggCCCTGCTGGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAACCGCCGAAAAAGGCCCGTACCACCCTCggg                    <  7:194789/133‑1 (MQ=255)                                                                                                             aagGCCCTGCTGGGGCAGCAGCAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAACCGCCGAAAAAGGCCCGTACCACCCTCGGGc                   <  5:197854/136‑1 (MQ=255)                                                                                                               ggCCCTGCTGGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAACCGCCGAAAAAGGCCCGTACCACCCTCGGGcc                  >  6:132544/1‑135 (MQ=255)                                                                                                                 cccTGCTGGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAACCGCCGCAACAGGCCCGTACCACCCTCGGGCCGAt               >  2:344097/1‑136 (MQ=255)                                                                                                                       tgGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGCCCGGGCCCGTGAGCCTGCAGAGCCAGTGGAACCGCCGAAAAAGGCCCGTACCACCCTCGGGCCGATGGGTTc         >  8:73996/1‑136 (MQ=255)                                                                                                                       tgGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAACCGCCGAAAAAGGc                                    <  6:165617/109‑1 (MQ=255)                                                                                                                       tgGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAACCGCCGAAAAAGGc                                    >  5:165617/1‑109 (MQ=255)                                                                                                                       tgGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAACCGCCGAAAAAGGCCCGTACCACCCTCGGGCCGATGGTTTc         >  7:78257/1‑136 (MQ=255)                                                                                                                       tgGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAACCGCCGAAAAAGGCCCGTACCACCCTCGGGCCGATGGTTTc         >  8:159000/1‑136 (MQ=255)                                                                                                                              gcagGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAACCGCCGAAAAAGGCCCGTACCACCCTCGGGCCGATGGGTTCCAGCGAt  >  6:116662/1‑136 (MQ=255)                                                                                                                                         |                                                                                                                              AGAAGCTGCAAGCGCGCGAACAGGACCAGGCCGACCTGGCCAAGGTCTTGAAGACCATCGAGGAAACCCTCGCCCGCCAGGCCCGTG‑AAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGGAAAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAACCGCCGAAAAAGGCCCGTACCACCCTCGGGCCGATGGTTTCCAGCGAT  >  ACB143/5760458‑5760718

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑