Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1432,171,2710GC35.6% 78.5 / 15.4 55V150V (GTG→GTC) CJ019_02060IS1182 family transposase ISPpu16
Reads supporting (aligned to +/- strand):  ref base G (24/5);  new base C (0/16);  total (26/29)
Fisher's exact test for biased strand distribution p-value = 3.15e-08
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

ACGGTAAAGCCATTCGTAGCGTCTGCCGTCAGTTCGTGATCCTCTGCCGTAA‑CCTCAACCTTTTCTCTGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAAGCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGATTCGGCAGACCGGGCAATGCCGGAGGTGGCCATG  >  ACB143/2171162‑2171397
                                                                                                              |                                                                                                                              
actgTAAAGCCATTCGTAGCGTCTGCCGTCAGTTCGTGATCCTCTGCCGTAA‑CCTCACCCTTTTCTCTGAGTCTATCATTGCCATCGACGGCAGCAAGTTCAAAGCAGTAAACAATCGCGACCGTAACTTCACCCa                                                                                                      <  6:200202/133‑1 (MQ=255)
  ggTAAACCAATTCGAAGCTTCTCCCTTCTGTGCGTGACCCTATGCCTTAA‑CATCAACCTTTTATATTAGTCGATAATTCCAATCGACGGCAGCACTTTCAAAGCAGTCAACAAACGCGACCGTAACTTCACCCAgg                                                                                                    <  2:296508/136‑1 (MQ=255)
         ccATTCGTAGCGTCTGCCGTCAGTTCGTGATCCTCTGCCGTAA‑CCTCAACCTTTTCTCTGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAAGCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGt                                                                                             >  5:217840/1‑136 (MQ=255)
          cATTCGTAGCGTCTGCCGTCAGTTCGTGATCCTCTGCCGTAA‑CCTCAACCTTTTCTCTGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAAGCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGt                                                                                             >  3:27231/1‑135 (MQ=255)
               gTAGCGTCTGCCGTCAGTTCGTGATCCTCTGCCGTAA‑CCTCAACCTTTTCTCTGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAAGCAGTGAACAATCGCGACCGTAACTTCCCCCAGGCCAAGGTGAAGGc                                                                                       >  8:81398/1‑136 (MQ=255)
               gTAGCGTCTGCCGTCAGTTCGTGATCCTCTGCCGTAA‑CCTCAACCTTTTCTCTGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAAGCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGc                                                                                       >  1:282236/1‑136 (MQ=255)
               gTAGCGTCTGCCGTCAGTTCGTGATCCTCTGCCGTAA‑CCTCAACCTTTTCTCTGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAAGCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGc                                                                                       >  3:200252/1‑136 (MQ=255)
               gTAGCGTCTGCCGTCAGTTCGTGATCCTCTGCCGTAA‑CAGCAACCTTTTCTCTGAGTCGTTCATTGCCATAGACGGCAGCAAGTTCAAAGCAGTGACCCATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGc                                                                                       >  8:306832/1‑136 (MQ=255)
                     tctcCCGTCAGTTCGGCAGCCGCTGCCGTAA‑CCTCAACCTTTTCTCTTAGTCGATCATTCCCAGCCACGGCAGAAAGTTCAACGCCGTCAACAAACGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCAt                                                                                 <  6:50341/136‑1 (MQ=255)
                      cTGCCGTCAGTTCGTGATCCTCTGCCGTAA‑ACTCAACCTTTTCTCTGAGTCGATCATTGCCATAGACGGCATCAACTTCAAAGCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGCTGAAGGCCCGCATg                                                                                >  5:162077/1‑136 (MQ=255)
                        gCCGTCAGTTCGTGATCCTCTGCCGTAA‑CCTCAACCTTTTCTCTGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAAGCAGTGAACAATAGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATg                                                                                >  1:261304/1‑134 (MQ=255)
                        gCCGTCAGTTCGTGATCCTATGCCGTAA‑CCTCAACCTTTTCTCTGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAAGCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATg                                                                                >  8:159249/1‑134 (MQ=255)
                            gatgTTCGTGTTCCTCTCCTGTAA‑CATCAACGTTTTATATGTTTCTTTCATTGCAATCGAGGGACTCATTTTCAACGCTGTTAACAATCGCGACCGTAACTTCACCCAGGCCAAGTTGAAGGCCCGCATGCAGCaa                                                                          <  4:245189/133‑1 (MQ=255)
                                ttCGTGATCCTCTGCCGAAA‑CCTCAAACTTTTCTCTGCGTCGATCATTGCCAGCGACGGCAGAAAGTTCAAAGCCGTCAACAAACGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCg                                                                      <  3:196325/136‑1 (MQ=255)
                                 tCGTTACCCTCTG‑CGTAACCCACAAACTTTTATCTTAGGCGATCATTGCCATCGAGGGCAGAAAGTAAAAACCATTTAAAAAGCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCg                                                                      <  2:118729/135‑1 (MQ=255)
                                 tCGTGATCCTCTGCCGTAA‑CCTCAACCTTTTCTCTGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAAGCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATc                                                                       >  5:36484/1‑134 (MQ=255)
                                  gtttaTCGTCTGCCTAAC‑CCTCAACCTTTTCTTTCAGGCCATAATTCCACTCGACGGCAGAATGTTCAAACCAGTCAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCga                                                                     <  7:294835/131‑1 (MQ=255)
                                  cGTGATCCTCTGCCTTAA‑CCTCAACATTTTATCTGAGTCGATATTTGCCATCGACGGCAGCAAGTTCAAAGCAGTTAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCga                                                                     <  4:210950/135‑1 (MQ=255)
                                  cGTGATCCTCTGCCGTAA‑CCTCAACCTTTTCTCTGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAAGCAGTGAACAATCGCGACCGTAACTTCACACAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCgag                                                                    >  7:191209/1‑136 (MQ=255)
                                       tcgcctGCCTAAA‑CCACAACTTTTTCTCTGAGTCGATATTGGCCATCCAGGGCATCAAGTTAAAACCAGTCAAAAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCgagcaga                                                                <  6:100131/131‑1 (MQ=255)
                                       tCCTCTGCCGTAA‑CCTCAACCTTTTCTCTGAGTCTATCATTGCCATCGAGGGCAGCAAGTTCAAAGCAGTCAACAACCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCgagcaga                                                                <  6:154870/135‑1 (MQ=255)
                                       tCCGCTGCCGTAC‑CATCAACTTTTTCTGTTCGTATTTCATTCCCAGCGCGGGCTGCAAGTTCAAAGCAGTTAACAACCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCgagcaga                                                                <  7:95546/135‑1 (MQ=255)
                                         ctgtGCCGAAA‑CCTAAACCTTTTCTCTGTGTCTATCATTGCCATCGACGGCAGCAAGTTCAAGGCAGTCAAAAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCgagcagagc                                                              <  7:164632/132‑1 (MQ=255)
                                           ctGCCGTAA‑CCTCAACCTTTTCTCTGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAAGCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCAtt                                                           >  3:254068/1‑136 (MQ=255)
                                             gcggTAC‑CCTCAACCTTTTCTCTGAGTCCAGCTTTCCCAGCGACGCCTCCAAGTTAAAAGCCGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGa                                                         <  6:336123/133‑1 (MQ=255)
                                             gcctaaa‑CCTCAACCTTTTCTGTGAGTCGAACATTCCCACCGACGGCTGCAAGTGCAAAGCTGTCAACAAGCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGa                                                         <  3:326261/131‑1 (MQ=255)
                                             gCCGTAA‑CCTCAACCTTTTCTCTGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAAGCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCAtt                                                           >  1:58261/1‑134 (MQ=255)
                                             gCCCTAA‑CCTCAACCTTTTCTCTGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAAGCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGa                                                         <  8:47555/136‑1 (MQ=255)
                                             cccGAAA‑CCACACCATTTTCTCGGAGTCTATCATTCCCATCCACGGCACCAAGATAAAACCCGCAAAAAAACGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGa                                                         <  7:145752/135‑1 (MQ=255)
                                              cgttaa‑cctaaccTTTTTCTCTGAGGCTATCTTTGCAATCGCCGGCATAATTTACAAAGCAGTCAAAAAGCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGa                                                         <  2:282236/132‑1 (MQ=255)
                                               cGTAA‑CCTAAACCTTTACTCTGAGTCTATCATTGCCATCGACGGCAGCAATTTCAAACAAGTCAACAAACGCGACCATAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGa                                                         <  2:134170/134‑1 (MQ=255)
                                                 tAA‑CCTCAACCTTTTCTCTGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAAGCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAgcg                                                      >  5:91317/1‑135 (MQ=255)
                                                 tAA‑CCTCAACCTTTGCTCTGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAAGCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAgcg                                                      >  1:180118/1‑135 (MQ=255)
                                                   acccTCAACCTTTTCTGTGCGTCGATCATGGCACTGGAGGGCAGCAAGTTCACAGCTGTCACCAAACGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCt                                                    <  7:145148/134‑1 (MQ=255)
                                                       tCAACCTTTTCTCTGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAAGCAGTGAACAATCGCGACAGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCt                                                >  5:183544/1‑136 (MQ=255)
                                                        aaaCCTTTTATCTGCGTCTATCTTTCCCAGCCCCGGCACACAGTTAAACGCTCTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCtt                                               <  8:61791/135‑1 (MQ=255)
                                                          aCCTTTTCTCTGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAAGCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTa                                              >  2:36342/1‑135 (MQ=255)
                                                            cTTTTCTCTGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAAGCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAg                                           >  6:291823/1‑136 (MQ=255)
                                                            cTTTTCTCTGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAAGCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAg                                           >  1:323721/1‑136 (MQ=255)
                                                                         ctaTCATTCCCAGCGAGGGAAGCAAGTACAAACCATTCAAAAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGATTCGGc                              <  5:134305/134‑1 (MQ=255)
                                                                         cGATCATTGCCATCGACGGCAGCAAGTTCAAAGCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGATTCgg                               >  1:323694/1‑135 (MQ=255)
                                                                              aTTGCCATCGACGGCAGCAAGTTCAAAGCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGATTCGGCAGAcc                         >  4:215637/1‑136 (MQ=255)
                                                                              aTTGCCATCGACGGCAGCAAGTTCAAAGCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGATTCGGCAGAcc                         >  7:26797/1‑136 (MQ=255)
                                                                               ttcccATCGACGGCAGAAAGTTCAAAGCAGTTAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGATTCGGCAGACCg                        <  2:284403/133‑1 (MQ=255)
                                                                                   cATCGACGGCAACAAGTTCAAAGCAGATAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAAACGACCAGAGCATAGAGCGCTACCTTACAGCGATGGATGCGGCAGACCGGGc                     >  4:242882/1‑135 (MQ=255)
                                                                                    aTCCACGGCAGCAAGTCCAAAGCATTCAAAAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGATTCGGCAGACCGGGCaa                   <  1:75121/136‑1 (MQ=255)
                                                                                        aCGGCAGCAAGTTCAAAGCAGTTAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAAACGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGATTCGGCAGACCGGGCAATGcc               >  5:200058/1‑136 (MQ=255)
                                                                                         cGGCAGCAAGTTCAAAGCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGATTCGGc                              <  7:49962/120‑1 (MQ=255)
                                                                                         cGGCAGCAAGTTCAAAGCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGATTCGGc                              >  8:49962/1‑120 (MQ=255)
                                                                                          ggCACCAAGTACAAACCAGTAACAAACCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGATTCGGCAGACCGGGCAATGCCgg             <  2:186492/136‑1 (MQ=255)
                                                                                           gcagcaAGTGCAAAGCTGCGAACATCCGCGACAGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGATTCGGCAGACCGGGCAATGCCGGa            <  2:74734/136‑1 (MQ=255)
                                                                                               aaaGTTCAACGCAGTCAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGATTCGGCAGACCGGGCAATGCCGGAGGTg        <  7:81398/135‑1 (MQ=255)
                                                                                                 ttttCACAGCCGTCAACCACCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGATTCGGCAGACCGGGCAATGCCGGAGGTg        <  1:122386/132‑1 (MQ=255)
                                                                                                  gTTCAAAGCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGATTCGGCAGACCGGGCAATGCCGGAGGTGGc      >  8:34157/1‑135 (MQ=255)
                                                                                                     caacccAGTCAACAACCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGATTCGGCAGACCGGGCAATGCCGGAGGTGGCCATg  <  7:263310/131‑1 (MQ=255)
                                                                                                              |                                                                                                                              
ACGGTAAAGCCATTCGTAGCGTCTGCCGTCAGTTCGTGATCCTCTGCCGTAA‑CCTCAACCTTTTCTCTGAGTCGATCATTGCCATCGACGGCAGCAAGTTCAAAGCAGTGAACAATCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGATTCGGCAGACCGGGCAATGCCGGAGGTGGCCATG  >  ACB143/2171162‑2171397

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑