| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | ACB143 | 4,425,070 | 0 | G | C | 42.3% | 26.3 / 14.6 | 26 | intergenic (‑29/‑89) | CJ019_04200/CJ019_04201 | hypothetical protein/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base G (14/1); new base C (0/11); total (14/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.55e-06 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GCGCAGTCGGCTTGCCGCCACGCGCCGTTTCCATGGTCGCAGAAGCACAACCGGCCAGCATCAGCACCGCAGCCAACGGCAGAATCGCACGCATGTTCCATCCTCGGGGTCGCAAAAAA‑GAAGGCGACTCTAGCACTGGCAGGCATCCCGAGTGAATTGCCGCGCACTTGCCGCTGTCGCAGATAAAACATGCCATCACGAGGAACCCATCATGAAACTGCACTATCTGCTACTCGCCGCCCTGC > ACB143/4424948‑4425192 | gcgcAGTCGGCTTGCCGCCACGCGCCGTTTCCATGGTCGCAGAAGCACAACCGGCCAGCATCAGCACCGCAGCCAACGGCAGAATCGCACGCATGTTCCATCCTCGGGGTCGCAAAAAA‑GAAGGCGACTCTAGCACt > 4:96184/1‑137 (MQ=255) aGTCGGCTTGCCGCCACGCGCCGTTTCCATGGTCGCAGAAGCACAACCGGCCAGCATCAGCACCGCAGCCAACGGCAGAATCGCACGCATGTTCCATCCTCGGGGTCGCAAAAAA‑GAAGGCGACTCTAGCACTggca > 5:44128/1‑137 (MQ=255) ccgccACGCGCCGTTTCCATGGTCGCAGAAGCACAACCGGCCAGCATCAGCACCGCAGCCAACGGCAGAATCGCCCGCATGTTCCATCCTCGGGGTCGCAAAAAA‑GAAGGCGACTCTAGCACTGGCAGGCATCCCGa > 5:205369/1‑137 (MQ=255) ccgccACGCGCCGTTTCCATGGTCGCAGAAGCACAACCGGCCAGCATCAGCACCGCAGCCAACGGCAGAATCGCACGCATGTTCCATCCTCGGGGTCGCAAAAAA‑GAAGGCGACTCTAGCACTGGCAGGCATCCCGa > 7:103105/1‑137 (MQ=255) gagctgTTTCCTGGGTCCAAAAACCACACCCGCCCAGCTTCCCCACCTCAACCAACGCTAGAATCTCCCGCATGTGCCATCTGCGGGG‑CTCAAAAAACCACGGCGACTCTAGCACTGGCAGGCATCCCGAGTGAAtt < 2:256462/132‑1 (MQ=255) cgcCGTTTCCATGGTCGCAGAAGCACAACCGGCCAGCATCAGCACCGCAGCCAACGGCAGAATCGCACGCATGTTCCATCCTCGGGGTCGCAAAAAA‑GAAGGCGACTCTAGCACTGGCAGGCATCCCGAGTGAATTg > 6:303112/1‑137 (MQ=255) gcCGTTTCCATGGTCGCAGAAGCACAACCGGCCAGCATCAGCACCGCAGCCAACGGCAGAATCGCACGCATGTTCCATCCTCGGGGTCGCAAAAAA‑GAAGGCGACTCTAGCACTGGCAGGCATCCCGAGTGAATTGc > 7:314666/1‑137 (MQ=255) gTTTCCATGGTCGCAGAAGCACACCCGGCCAGCATCACCACCGCAGCCACCGCCAGAACCCCCCGCATGTTCCATCCTCGGGGGCCCAAAAAC‑CACCGCGACTCTAGCACTGGCAGGCATCCCGAGTGAATTGCcgc < 7:50020/137‑1 (MQ=255) tGGTCGCACAACCACAACCGCCCAGCACCACCCCCGCACCCACCGCCATAAGCGCACGCATGTACCACCCTCGGGGGCGCAAAAAA‑CAACGCGACTCTAGCACTGGCAGGCATCCCGAGTGAATTGCCGCGCACTTg < 8:103105/137‑1 (MQ=255) gCAGAAGCACAACCGGCCAGCATCAGCACCGCAGCCAACGGCAGAATCGCACGCATGTTCCATCCTCGGGGTCGCAAAAAA‑GAAGGCGACTCTAGCACTGGCAGGCATCCCGAGTGAATTGCCGCGCACTTGCCGCt > 3:50584/1‑137 (MQ=255) aacaaccacaACCGGCCACCACCACCACCCCACCCAACGCCATAACCGCACGCTTTTACCTCCCGCGGGGCCCCAAAAAA‑CACCGCGACTCTAGCACTGGCAGGCATCCCGAGTGAATTGCCGCGCACTTGCCGCTg < 3:96184/131‑1 (MQ=255) aGAAGCACAACCGGCCAGCATCAGCACCGCAGCCAACGGCAGAATCGCACGCATGTTCCATCCTCGGGGTCGCAAAAAA‑GAAGGCGACTCTAGCACTGGCAGGCATCCCGAGTGAATTGCCGCGCACTTGCCGCTGt > 5:213614/1‑137 (MQ=255) acccGCCCAGCAGCCCCACCGCACACAACGGAATAATCGCACGCATGTACCAACCGCGGGGGCGAAAAAAA‑TAACGCGACTCTAGCACTGGCAGGCATCCCGAGTGAATTGCCGCGCACTTGCCGCTGTCGCAGATa < 6:174204/135‑1 (MQ=255) cccGGCCATCATCACCACCTCACCACAGGGCATACGCCCCCGCTTGTTCCTTCC‑GGGGGTACCAAAAAACAAACGCGACTCTAGCACTGGCAGGCATCCCGAGTGAATTGCCGCGCACTTGCCGCTGTCGCAGATaa < 8:203314/136‑1 (MQ=255) cgCCAGAAACACCCCCGCACCCAACGGCATAATCGCACGCTTGTTCCAACCTGGGTGTCGCAAAAAA‑CAACGCGACTCTAGCACTGGCAGGCATCCCGAGTGAATTGCCGCGCACTTGCCGCTGTCGCAGATAAAAc < 8:124577/136‑1 (MQ=255) cATCAGCACCGCAGCCAACGGCAGAATCGCACGCATGTTCCATCCTC‑GGGTCGCAAAAAA‑GAAGGCGACTCTAGCACTGGCAGGCATCCCGAGTGAATTGCCGCGCACTTGCCGCTGTCGCAGATAAAACATGCCAt > 1:161833/1‑137 (MQ=255) cGCAGCCAACGGCAGAATCGCACGCATGTTCCATCCTCGGGGTCGAAAAAAA‑GAACGCGACTCTAGCACTGGCAGGCATCCCGAGTGAATTGCCGCGCACTTGCCGCTGTCGCAGATAAAACATGCCATCACGAGGa < 7:301929/137‑1 (MQ=255) ccAACGGCAGAATCGCACGCATGTTCCATCGTCGGGGGCCAAAAAAA‑GAACGCGACTCTAGCACTGGCAGGCATCCCGAGTGAATTGCCGCGCACTTGCCGCTGTCGCAGATAAAACATGCCATCACGAGGAACCca < 5:321681/137‑1 (MQ=255) ccAACGGCAGAATCGCACGCATGTTCCATCCTCGGGGTCGCAAAAAA‑GAAGGCGCCTCTAGCACTGGCAGGCATCCCGAGTGAATTGCCGCGCACTTGCCGCTGTCGCAGATAAAACATGCCATCACGAGGAACCca > 1:156287/1‑137 (MQ=255) caaccGGCAGATTCCCACTCTTGTCCCACCCTCGGGGTCCCAAAAAA‑CACCGCGACTCTAGCACTGGCAGGCATCCCGAGTGAATTGCCGCGCACTTGCCGCTGTCGCAGATAAAACATGCCATCACGAGGAACCca < 1:88179/133‑1 (MQ=255) aGAATCGCACGCATGTTCCATCCTCGGGGTCGCAAAAAA‑GAAGGCGACTCTAGCACTGGCAGGCATCCCGAGTGAATTGCCGCGCACTTGCCGCTATCGCAGATAAAACATGCCATCACGAGGAACCCATCATGaaa > 2:12760/1‑137 (MQ=255) gcaTGTTCCATCCTCGGGGTCGCAAAAAA‑GAAGGCGACTCTAGCACTGGCAGGCATCCCGAGTGAATTGCCGCGCACTTGCCGCTGTCGCAGATAAAACATGCCATCACGAGGAACCCATCATGAAACTGCACTATc > 2:110607/1‑137 (MQ=255) cgtCGGGGCCGCAAAAAA‑GACCGCGACTCTAGCACTGGCAGGCATCCCGAGTGAATTGCCGCGCACTTGCCGCTGTCGCAGATAAAACATGCCATCACGAGGAACCCATCATGAAACTGCACTATCTGCTACTcgcc < 7:322924/135‑1 (MQ=255) gggtTCGAAAAAAA‑GAACGCGACTCTAGCACTGGCAGGCATCCCGAGTGAATTGCCGCGCACTTGCCGCTGTCGCAGATAAAACATGCCATCACGAGGAACCCATCATGAAACTGCACTATCTGCTACTCGCCGccc < 6:213614/137‑1 (MQ=255) ggggTCGCAAAAAA‑GAAGGCGACTCTAGCACTGGCAGGCATCCCGAGTGAATTGCCGCGCACTTGCCGCTGTCGCAGATAAAACATGCCATCACGAGGAACCCATCATGAAACTGCACTATCTGCTACTCGCCGccc > 2:294738/1‑137 (MQ=255) gTCGCAAAAAA‑GAAGGCGACTCTAGCACTGGCAGGCACCCCGAGTGAATTGCCGCGCACTTGCCGCTGTCGCAGATAAAACATGCCATCACGAGGAACCCATCATGAAACTGCACTATCTGCTACTCGCCGCCCtgc > 2:275460/1‑137 (MQ=255) | GCGCAGTCGGCTTGCCGCCACGCGCCGTTTCCATGGTCGCAGAAGCACAACCGGCCAGCATCAGCACCGCAGCCAACGGCAGAATCGCACGCATGTTCCATCCTCGGGGTCGCAAAAAA‑GAAGGCGACTCTAGCACTGGCAGGCATCCCGAGTGAATTGCCGCGCACTTGCCGCTGTCGCAGATAAAACATGCCATCACGAGGAACCCATCATGAAACTGCACTATCTGCTACTCGCCGCCCTGC > ACB143/4424948‑4425192 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |