Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1435,530,5250AT21.4% 112.9 / 11.2 56*379K (TAA→AAA) braC_2Leucine‑, isoleucine‑, valine‑, threonine‑, and alanine‑binding protein
Reads supporting (aligned to +/- strand):  ref base A (11/33);  new base T (11/1);  total (22/34)
Fisher's exact test for biased strand distribution p-value = 4.41e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

CACGAGCGCCGACCACGGCGCGGGCCACCTGTGCGGGGCCCTCGCAGCGCAGAACTGTGCAGATCTACGAATAAAGACAGGTACCGCGGGGCTTGGGCCCGAGCAACCGCCCGGGCCGCGGGCCGT‑TGTTATTTTTGTTTTTCCAGCTGGTGGTATTTACCGGTCTTGTCCCACTGGTAGACCACGTAGTCGGAGACGGTCAGGTCGCCCTTGCTGTCCCACTTCTTCTCACCCATGACGGTCTGCACCGGGTTGGCCTT  >  ACB143/5530395‑5530654
                                                                                                                                   |                                                                                                                                 
cACGAGCGCCGACCACGGCGCGGGCCACCTGTGGCGGGCCCTCGGCGCGCAGAACTGTGCAGATGTAAGAATAAAGACAGGTACCGCGGGGCTTGGGCCCGAGCAAACCACCGGGCCGCGGGGCGTGTGtttttttt                                                                                                                              >  6:238607/1‑137 (MQ=255)
  cGAGCGCCGACCACGGCGCGGGCCACCTGTGCGGGGCCCTCGCAGCGCAGAACTGTGCAGATCTACGAATAAAGACAGGTACCGCGGGGCTTGGGCCCGAGCAACCGCCCGGGCCGCGGGCCGT‑TGTTATTTTTGtt                                                                                                                           <  6:356568/137‑1 (MQ=255)
   gAGCGCCGACCACGGCGCGGGCCACCTGTGCGGGGCCCTCGCAGCGCAGGACTTTGGAGATCTACGAATAAAGACAGGTACCCCGGGGCTTGGGCCCGCGCAACCGCCCGGGCCGCGGGCCGG‑TGGGTTTTTTGttt                                                                                                                          >  5:115892/1‑137 (MQ=255)
         cGACCCCGGCGCGGGCCACCTGTGCGGGGCCCTCGCAGCGCAGAACTGTGCAGATCTACGAATAAAGACAGGTACCGCGGGGCTTGGGCCCGAGCAACCGCCCGGGCCGCGGGCCGT‑TGTTATTTTTGTTTTTCCAg                                                                                                                    <  5:92860/137‑1 (MQ=255)
         cGACCACGGCGCGGGCCACCTGTGCGGGGCCCTCGCAGCGCCGAACTGTGCAGATCTACGAATAAAGACAGGTACCGCGGGGCTTGGGCCCGAGCAACCGCCCGGGCCGCGGGCCCT‑GGGTATTTTTGtttttggag                                                                                                                    >  7:180981/1‑133 (MQ=255)
         cGACCACGGCGCGGGCCACCTGTGCGGGGCCCTCGCAGCGCAGAACTGTGCAGATCTACGAATAAAGACAGGTACCGCGGGGCTTGGGCCCGAGCAACCGCCCGGGCCGCGGGCCGT‑TGTTATTTTTGTTTTTCCAg                                                                                                                    <  7:241530/137‑1 (MQ=255)
          gACCCCGGCGCGGGCCACCTGGGCGGGGCCCTCGCAGCTCAGAACTGTGCAGATCTACGAATAAAGACAGGTACCGCGGGGCTTGGGCCCGAGCAACCGCCCGGGCCGCGGGCCGT‑TGTTATTTTTGTTTTTCCAGc                                                                                                                   <  8:350718/137‑1 (MQ=255)
              ccGGCGCGGGCCACCTGTGCGGGGCCCTCGCAGCGCAGAACTGTTCAGATCTACGAATAAAGACAGGTACCGCGGGGCTTGGGCCCGAGCAACCGCCCGGGCCGCGGGCCGT‑TGTTATTTTTGTTTTTCCAGCtggt                                                                                                               <  6:13635/136‑1 (MQ=255)
                 gcgcGGGCCACCTGTGCGGGGCCCTCGCAGCGCAGAACTGTGCAGATCTACGAATAAAGACAGGTACCGCGGGGCTTGGGCCCGAGCAACCGCCCGGGCCGCGGGCCGT‑TGTTATTTTTGTTTTTCCAGCTggtggt                                                                                                            <  3:349922/137‑1 (MQ=255)
                    cgGGCCACCTGTGCGGGGCCCTCGCAGCGCAGAACTGTGCAGATCTACGAATAAAGACAGGTACCGCGGGGCTTGGGCCCGAGCAACCGCCCGGGCCGCGGGCCGT‑TGTTATTTTTGTTTTTCCAGCTGGTGGTAtt                                                                                                         <  7:98682/137‑1 (MQ=255)
                      ggCCACCTGTGCGGGGCCCTCGCAGCGCAGAACTGTGCAGATCTACGAATAAAGACAGGTACCGCGGGGCTTGGGCCCGAGCAACCGCCCGGGCCGCGGGCCGT‑TGTTATTTTTGTTTTTCCAGCTGGTGGTATTTa                                                                                                       <  4:30171/137‑1 (MQ=255)
                           ccTGTGCGGGGCCCTCGCAGCGCAGAACTGTGCAGATCTACGAATAAAGACAGGTACCGCGGGGCTTGGGCCCGAGCAACCGCCCGGGCCGCGGGCCGT‑TGTTATTTTTGTTTTTCCAGCTGGTGGTATTTACCGGt                                                                                                  <  4:174836/137‑1 (MQ=255)
                               tgCGGGGCCCTCGCAGCGCAGAACTGTGCAGATCTACGAATAAAGACAGGTACCGCGGGGCTTGGGCCCGAGCAACCGCCCGGGCCGCGGGCCGT‑TGTTATTTTTGTTTTTCCAGCTGGTGGTATTTACCGGTCTTg                                                                                              <  6:135458/137‑1 (MQ=255)
                               ggCGGGGCCCTCGCAGCGCAGAACTGTGCAGATCTACGAATAAAGACAGGTACCGCGGGGCTTGGGCCCGAGCAACCGCCCGGGCCGCGGGCCGT‑TGTTATTTTTGTTTTTCCAGCTGGTGGTATTTACCGGTCTTg                                                                                              <  7:193674/136‑1 (MQ=255)
                                  ggggCCCTCGCAGCGCAGAACTGTGCAGATCGACGAATAAAGACAGGTACCGCGGGGCTTGGGCCCGAGCAACCGCCCGGGCCGCGGGCCGT‑TGTTTTTTTTTTTTTTCCAGCTGGTGGTATTTACCGGTCTTGTcc                                                                                           >  8:55981/1‑137 (MQ=255)
                                    ggCGCTCGCAGCGCATAACTGTGCAGATCTACGAATAAAGACAGGTACCGCGGGGCTTGGGCCCGAGCAACCGCCCGGGCCGCGGGCCGT‑TGTTATTTTTGTTTTTCCAGCTGGTGGTATTTACCGGTCTTGTCCCa                                                                                         <  1:372112/137‑1 (MQ=255)
                                    ggCCCTCGCAGCGCAGAACTGTGCAGATCTACGAATAAAGACAGGTACCGCGGGGCTTGGGCCCGAGCAACCGCCCGGGCCGCGGGCCGT‑GGGTATTTTTGTTTTTTCAGCTGGTGGTATTTACAGGTCTTGTgccc                                                                                         >  5:99789/1‑136 (MQ=255)
                                      cccTCGCAGCGCAGAACTGTGCAGATCTACGAATAAAGACAGGTACCGCGGGGCTTGGGCCCGAGCAACCGCCCGGGCCGCGGGCCGT‑TGTTATTTTTGTTTTTCCAGCTGGTGGTATTTACCGGTCTTGTCCCACt                                                                                       <  3:13300/137‑1 (MQ=255)
                                         tCGCAGCGCAGAACTGTGCAGATCTACGAATAAAGACAGGTACCGCGGGGCTTGGGCCCGAGCAACCGCCCGGGCCGCGGGCCGT‑TGTTATTTTTGTTTTTACAGCTGGTGGTATTTAACGGTCTTGTCCCACTGGt                                                                                    >  5:170398/1‑137 (MQ=255)
                                          cgcagcgcagAACTGTGCAGATCTACGAATAAAGACAGGTACCGCGGGGCTTGGGCCCGAGCAACCGCCCGGGCCGCGGGCCGT‑TGTTATTTTTGTTTTTCCAGCTGGTGGTATTTACCGGTCTTGTCCCACTGGTa                                                                                   <  1:224300/137‑1 (MQ=255)
                                              gcgcagAACTGTGCAGATCTACGAATAAAGACAGGTACCGCGGGGCTTGGGCCCGAGCAACCGCCCGGGCCGCGGGCCGT‑TGTTATTTTTGTTTTTCCAGCTGGTGGTATTTACCGGTCTTGTCCCACTGGTAGAcc                                                                               <  7:349938/137‑1 (MQ=255)
                                              gcgcagAACTGTGCAGATCTACGAATAAAGACAGGTACCGCGGGGCTTGGGCCCGAGCAACCGCCCGGGCCGCGGGCCGT‑TGTTATTTTTGTTTTTCCAGCTGGTGGTATTTACCGGTCTTGGCCCACTGGTAGAcc                                                                               <  8:113840/137‑1 (MQ=255)
                                                 cagAACTGTGCAGATCTACGAATAAAGACAGGTACCGCGGGGCTTGGGCCCGAGCAACCGCCCGGGCCGCGGGCCGT‑TGTTATTTTTGTTTTTCCAGCTGGTGGTATTTACCGGTCTTGTCCCACTGGTAGACCACg                                                                            >  5:159234/1‑137 (MQ=255)
                                                  agAACTGTGCAGATCTACGAATAAAGACAGGTACCGCGGGGCTGGGGCCCGAGCAACCGCCCGGGCCGCGGGCCGT‑TGTTATTTTTGTTTTTCCAGCTGGTGGTATTTACCGGTCTTGTCCCACTGGTAGACCACGt                                                                           <  5:62009/137‑1 (MQ=255)
                                                       tgtgCAGATCTACGAATATAGACCGGGACAGCGGGGCTTGGTCCCGAGCAACCGCCCGGGCCGCGGGCCGT‑TGTTATTTTTGTTTTTCCAGCTGGTGGTATTTACCGGTGTTGTCCCACTGGTAGACCACGTAGTCg                                                                      <  5:234755/137‑1 (MQ=255)
                                                       tgtgCAGATCTACGAATAAAGACAGGTACCGCGGGGCTTGGGCCCGAGCAACCGCCGGGGCCGCGGGCCGT‑TGTTATTTTTGTTTTTCCAGCTGGTGGTATTTACCGGTCTTGTCCCACTGGTAGACCACGTAGTCg                                                                      >  5:167066/1‑137 (MQ=255)
                                                       tgtgCAGATCTACGAATAAAGACAGGTACCGCGGGGCTTGGGCCCGAGCAACCGCCCGGGCCGCGGGCCGT‑TGTTTTTTTTGTTTTTCCAGCTGGTGGTATTTACCGGTCTTGTCCCACTGGTTGACCCCGGAGTCg                                                                      >  8:163828/1‑137 (MQ=255)
                                                       tgtgCAGATCTACGAATAAAGACAGGTACCGCGGGGCTTGGGCCCGAGCAACCGCCCGGGCCGCGGGCCGT‑TGTTTTTTTTGTTTTTCCAGCTGGTGGTATTTACCGGTCTTGTCCCACTGGTAGACCACGTAGTCg                                                                      <  5:349138/137‑1 (MQ=255)
                                                            aGATCTACGAATAAAGACAGGTACCGCGGGGCTTGGGCCCGAGCAACCGCCCGGGCCGCGGGCCGT‑TGTTTTTTTTGTTTTTGCAGCTGGTGGTATTTACCGGTCTTTTGCCCCTGGTAGACCACGTAGTGGGAGAc                                                                 >  3:180381/1‑137 (MQ=255)
                                                            aGATCTACGAATAAAGACAGGTACCGCGGGGCTTGGGCCCGAGCAACCGCCCGGGCCGCGGGCCGT‑TGTTATTTTTTTTTTTCCAGCTGGTGGTATTTACCGGTCTTGTCCCACTGGTAGAACACGTAGTAGGAGAc                                                                 >  3:125031/1‑137 (MQ=255)
                                                            aGATCTACGAATAAAGACAGGTACCGCGGGGCTTGGGCCCGAGCAACCGCCCGGGCCGCGGGCCGT‑GGTTTTTTTTGTTTTTACAGCTGGTGGTATTTACCGGTCTTGTCCCCCTGGTAGCCCACGTAGTCGGCGAc                                                                 >  5:100892/1‑137 (MQ=255)
                                                            aGATCTACGAATAAAGACAGGTACCGCGGGGCTTGGGCCCGAGCAACCGCCCGGGCCGCGGGCCGT‑GGGTTTTTTTTTTTTTGCAGCTGGGGGTTTTTAACGGGGTTGTGCCCCTGGTATCCCCCGTTGTCGGAGAc                                                                 >  5:236640/1‑137 (MQ=255)
                                                              aTCTACGAATAAAGACAGGTACAGCGGGGCTTGGGCCCGAGCAACCGCCCGGGCCGCGGGCCGT‑GGTTTTTTTTTGTTTTACCCCTGGTGGTAATTTCCCGTCTTGTCCCACTGGTAGACCACGTATACGGAGAAgg                                                               >  7:101772/1‑137 (MQ=255)
                                                                 aaCGAATAAAGACAGGTACCGCGGGGCTTGGGCCCGAGCAACCGCCCGGGCCGCGGGCCGT‑TGTTATTTTTGTTTTTCCAGCTGGTGGTATTTACCGGTCTTGTCCCACTGGTAGACCACGTAGTCGGAGACGGTCa                                                            <  1:149390/136‑1 (MQ=255)
                                                                   cGAATAAAGACAGGTACCGCGGGGCTTGGGCCCGAGCAACCGCCCGGGCCGCGGGCCGT‑TGTTATTTTTGTTTTTCCAg                                                                                                                    >  7:92482/1‑79 (MQ=255)
                                                                   cGAATAAAGACAGGTACCGCGGGGCTTGGGCCCGAGCAACCGCCCGGGCCGCGGGCCGT‑TGTTATTTTTGTTTTTCCAg                                                                                                                    <  8:92482/79‑1 (MQ=255)
                                                                     aaTAAAGACAGGTACCGCGGGGCTTGGGCCCGAGCAACCGCCCGGGCCGCGGGCCGT‑TGTTATTTTTGTTTTTCCAGCTGGTGGTATTTACCGGTCTTGTCCCACTGGTAGACCACGTAGTCGGAGACGGTCAGGTc                                                        <  6:234511/137‑1 (MQ=255)
                                                                      aTAAAGACAGGTACCGCGGGGCTTGGGCCCGAGCAACCGCCCGGGCCGCGGGCCGT‑TGTTATTTTTGTTTTTGGAGCTGGTGGTATTTACCGGTCTTGTCCCACTGGTAGACCAAGTAGTCGGAGACGGTCAGGTCg                                                       >  7:76869/1‑137 (MQ=255)
                                                                      aTAAAGACAGGTACCGCGGGGCTTGGGCCCGAGCAACCGCCCGGGCCGCGGGCCGT‑TGTTATTTTTGTTTTTCCAGCTGGTGGTATTTACCGGTCTTGTCCCACTGGTAGACCACGTAGTCGGAGACGGTCAGGTCg                                                       >  7:341172/1‑137 (MQ=255)
                                                                           gccAGGTACCGCGGGGCTTGGGCCCGAGCAACCGCCCGGGCCGCGGGCCGT‑TGGTTTTTTTTTTTGTCCAGCTTGTGGTTTTTTACGGTGGTGTCGCCCAGGTTGTACACGTAGGAGGGGCCGGTCAGGGCGACCtt                                                  >  7:336091/3‑137 (MQ=255)
                                                                           gACAGGTACCGCGGGGCTTGGGCCCGAGCAACCGCCCGGGCCGCGGGCCGT‑TGGTTTTTTTTTTTTTTCAGCTGGTGGTGTTTAACGGGGATTTGCCACAGGTATACCACGTATTGGGAGACGGTGCGGTGGcccgt                                                  >  1:78440/1‑135 (MQ=255)
                                                                               ggTACCGCGGGGCTTGGGCCCGAGCAACCGCCCGGGCCGCGGGTCGT‑TGTTATTTTTGTTTTTCCAGCTGGTGGTATTTACCGGTCTTGTCCCACTGGTAGACCACGTAGTCGGAGACGGTCAGGTCGCCCTTGCTg                                              <  5:282447/137‑1 (MQ=255)
                                                                                  aCCGCGGGGCTTGGGCCCGAGCAACCGCCCGGGCCGCGGGCCGT‑TGTTATTTTTGTTTTTCCAGCTGGTGGTATTTACCGGTCTTGTCCCACTGGTAGACCACGTAGTCGGAGACGGTCAGGTCGCCCTTGCTGTcc                                           <  1:17704/137‑1 (MQ=255)
                                                                                  aCCGCGGGGCTTGGGCCCGAGCAACCGCCCGGGCCGCGGGCCGT‑TGTTAATTTTGTTTTTCCAGCTGGTGGTATTTACCGGTCTTGTCCCACTTGTAGACCTCGTATTCTGAGACGGTCAGGTCGCCCTTTCtttta                                           <  7:341989/137‑5 (MQ=255)
                                                                                   ccGCGGGGCTTGGGCCCGAGCAACCGCCCGGGCCGCGGGCCGT‑TGTTTTTTTTGTTTTTGCCGCTGGTGGTATTTTCCGGGCTTGTCCCACTGGTAGACCACGGATTCGGAGACGGTCAGGTCGCCCTTGCTGTccc                                          >  3:179431/1‑137 (MQ=255)
                                                                                   ccGCGGGGCTTGGGCCCGAGCAACCGCCCGGGCCGCGGGCCGT‑TGTTATTTTTGTTTTTCCAGc                                                                                                                   >  2:71854/1‑64 (MQ=255)
                                                                                   ccGCGGGGCTTGGGCCCGAGCAACCGCCCGGGCCGCGGGCCGT‑TGTTATTTTTGTTTTTCCAGc                                                                                                                   <  1:71854/64‑1 (MQ=255)
                                                                                   ccGCGGGGCTTGGGCCCGAGCAACCGCCCGGGCCGCGGGCCGG‑GGTTTTTTTTTTTTTTGGAGCTGGTGGTTTTTTACGGGGTTTTGCCACGGGTAGTCCCCGGTGTCGGGGACGGTCCGGTCGGCCGTGgtgtggc                                          >  2:140777/1‑134 (MQ=255)
                                                                                       gggtCTTGGTCCCGAGCAACCGCCCGGGCCGCGGGCCGT‑TGTTATTTTTGTTTTTCCAGCTGGTGGTATTTACCGGTCTTGTCCCACTGGTAGACCACGTAGTCGGAGACGGTCAGGTCGCCCTTGCTGTCCCActt                                      <  6:260138/137‑1 (MQ=255)
                                                                                          gCTTGGGCCCGAGCAACCGCCCGGGCCGCGGGCCGT‑TGTTATTTTTGTTTTTCCAGCTGGTGGTATTTACCGGTCTTGTCCCACTGGTAGACCACGTAGTCGGAGACGGTCAGGTCGCCCTTGCTGTCCCActtctt                                   <  8:61392/137‑1 (MQ=255)
                                                                                            ttGGGCCCGAGCAACCGCCCGGGCCGCGGGCCGT‑TGTTATTTTTGTTTTTCCAGCTGGTGGTATTTACCGGTCTTGTCCCACTGGTAGACCACGTAGTCGGAGACGGTCAGGTCGCCCTTGCTGTCCCACTtcttct                                 <  8:180981/137‑1 (MQ=255)
                                                                                                 cccGAGCAACCGCCCGGGCCGCGGGCCGT‑TGTTATTTTTGTTTTTCCAGCTGGTGGTATTTACCGGTCTTGTCCCACTGGTAGACCACGTAGTCGGAGACGGTCAGGTCGCCCTTGCTGTCCCACTTCTTCTCAccc                            <  7:248046/137‑1 (MQ=255)
                                                                                                 cccGAGCAACCGCCCGGGCCGCGGGCCGT‑TGTTATTTTTGTTTTTCCAGCTGGTGGTATTTACCGGTCTTGTCCCACTGGTAGACCACGTAGTCGGAGACGGTCAGGTCGCCCTTGCTGTCCCACTTCTTCTCAccc                            <  7:124125/137‑1 (MQ=255)
                                                                                                      gCAACCGCCCGGGCCGCGGGCCGT‑TGTTATTTTTGTTTTTCCAGCTGGTGGTATTTACCGGTCTTGTCCCACTGGTAGACCACGTAGTCGGAGACGGTCAGGTCGCCCTTGCTGTCCCACTTCTTCTCACCCATGat                       <  8:304370/137‑2 (MQ=255)
                                                                                                                 ggccgcgggccgT‑TGTTATTTTTGTTTTTCCAGCTGGTGGTATTTACCGGTCTTGTCCCACTGGTAGACCACGTAGTCGGAGACGGTCAGGTCGCCCTTGCTGTCCCACTTCTTCTCACCCATGACGGTCTGCACCg            <  6:167066/137‑1 (MQ=255)
                                                                                                                  gccgcgggccgT‑TGTTATTTTTGTTTTTCCAGCTGGTGGTATTTTCCGGTCTTGTCCCACTGGTAGACCACGTAGTCGGAGACGGTCAGGTCGCCCTTGCTGTCCCACTTCTTCTCACCCATGACGGTCTGCACCgg           >  6:153368/1‑137 (MQ=255)
                                                                                                                           ggt‑tgttATTTTTGTTTTTCCAGCTGGTGGTATTTACCGGTCTTGTCCCACTGGTAGACCACGTAGTCGGAGACGGTCAGGTCGCCCTTGCTGTCCCACTTCTTCTCACCCATGACGGTCTGCACCGGGTTGGCCtt  <  6:236640/136‑1 (MQ=255)
                                                                                                                                   |                                                                                                                                 
CACGAGCGCCGACCACGGCGCGGGCCACCTGTGCGGGGCCCTCGCAGCGCAGAACTGTGCAGATCTACGAATAAAGACAGGTACCGCGGGGCTTGGGCCCGAGCAACCGCCCGGGCCGCGGGCCGT‑TGTTATTTTTGTTTTTCCAGCTGGTGGTATTTACCGGTCTTGTCCCACTGGTAGACCACGTAGTCGGAGACGGTCAGGTCGCCCTTGCTGTCCCACTTCTTCTCACCCATGACGGTCTGCACCGGGTTGGCCTT  >  ACB143/5530395‑5530654

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑