Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB143 1,943,813 C→T 9.7% L230L (CTG→CTA)  asnB ← Asparagine synthetase [glutamine‑hydrolyzing] 1

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1431,943,8130CT 9.7% 122.8 / 4.4 41L230L (CTG→CTA) asnBAsparagine synthetase [glutamine‑hydrolyzing] 1
Reads supporting (aligned to +/- strand):  ref base C (16/21);  new base T (2/2);  total (18/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.29e-01

GGACAGCAACACACCCACATCGACGGCTGCCCGTTGGCGGATGGCTACGGCGTCGCGGGTGGCGTCGAGCACGCGGGTGGTCCAGCCTTCCAGGTCCAGTTCACGCTCGTCGGGGTGGGGGCCGTAGTGCAGTTGCCACCAGACCTGGCGTTCCACTTCGCCATGGCGGTCGATGCGCATCCAGGTGCCGGGCTCCAGCTTCTGCACGTTGGCCAGCAGGGTGCGCGGTGCCGGTACCACGGCGTGGAAATTCAGGTAGTGGTT  >  ACB143/1943684‑1943947
                                                                                                                                 |                                                                                                                                      
ggACAGCAACACACCCACATCGACGGCTGCCCGTTGGCGGATGGCTACGGCGTCGCGGGTGGCGTCGAGCACGCGGGTGGTCCAGCCTTCCAGGTCCAGTTCACGCTCGTCGGGGTGGGGGCCGTAGTGCAGTTGc                                                                                                                                  <  8:187359/136‑1 (MQ=255)
ggACAGCAACACACCCACATCGACGGCTGCCCGTTGGCGGATGGCTACGGCGTCGCGGGTGGCGTCGAGCACGCGGGTGGTCCAGCCTTCCAGGTCCAGTTCACGCTCGTCGGGGTGGGGGCCGTAGTGCAGTTGc                                                                                                                                  <  5:122163/136‑1 (MQ=255)
      cAACACACCCACATCGACGGCTGCCCGTTGGCGGATGGCTACGGCGTCGCGGGTGGCGTCGAGCACGCTGGTGGTCCAGCCTTCCAGGTCCAGTTCACGCTCGTCGGGGTGGGGGCCGTAGTGCAGTTGCCACCAg                                                                                                                            <  1:174807/136‑1 (MQ=255)
        acacacCCACATCGACGGCTGCCCGTTGGCGGATGGCTACGGCGTCGCGGGTGGCGTCGAGCACGCGGGTGGTCCAGCCTTCCAGGTCCAGTTCACGCTCGTCGGGGTGGGGGCCGTAGTGCAGTTGCCACCAGAc                                                                                                                          <  8:189340/136‑1 (MQ=255)
         cacacCCACATCGACGGCTGCCCGTTGGCGGATGGCTACGGCGTCGCGGGTGGCGTCGAGCACGCGGGTGGTCCAGCCTTCCAGGTCCAGTTCACGCTCGTCGGGGTGGGGGCCGTAGTGCAGTTGCCACCATAcc                                                                                                                         <  4:63927/136‑1 (MQ=255)
           cacCCACATCGACGGCTGCCCGTTGGCGGATGGCTACGGCGTCGCGGGGGGCGTCGAGCACGCGGGTGGTCCAGCCTTCCAGGTCCAGTTCACGCTCGTCGGGGTGGGGGCCGTAGTGCAGTTGCCACCAGACCTg                                                                                                                       <  1:72386/136‑1 (MQ=255)
               cacaTCGACGGCTGCCCGTTGGCGGATGGCTACGGCGTCGCGGGTGGCGTCGAGCACGCGGGTGGTCCAGCCTTCCAGGTCCAGTTCACGCTCGTCGGGGTGGGGGCCGTAGTGCAGTTGCCACCAGACCTGGCGt                                                                                                                   >  2:150964/1‑136 (MQ=255)
                 caTCGACGGCTGCCCGTTGGCGGATGGCTACGGCGTCGCGGGTGGCGTCGAGCACGCGGGTGGTCCAGCCTTCCAGGTCCAGTTCACGCTCGTCGGGGTGGGGGCCGTAGTGCAGTTGCCACCAGACCTGGCGTTc                                                                                                                 >  2:121533/1‑136 (MQ=255)
                   tCGACGGCTGCCCGTTGGCGGATGGCTACGGCGTCGCGGGTGGCGTCGAGCACGCGGGTGGTCCAGCCTTCCAGGTCCAGTTCACGCTCGTCGGGGTGGGGGCCGTAGTGCAGTTGCCACCAGACCTGGCGTTCCa                                                                                                               >  6:289757/1‑136 (MQ=255)
                         gCTGCCCGTTGGCGGATGGCTACGGCGTCGCGGGTGGCGTCGAGCACGCGGGTGGTCCAGCCTTCCAGGTCCAGTTCACGCTCGTCGGGGTGGGGGCCGTAGTGCAGTTGCCACCAGACCTGGGGTTCCACTTCGc                                                                                                         >  3:172109/1‑136 (MQ=255)
                           tGCCCGTTGGCGGATGGCTACGGCGTCGCGGGTGGCGTCGAGCACGCGGGTGGTCCAGCCTTCCAGGTCCAGTTCACGCTCGTCGGGGTGGGGGCCGTAGTGTAGTTGCCACCAGACCTGGCGTTCCACTTCGCCa                                                                                                       >  6:126905/1‑136 (MQ=255)
                           tGCCCGTTGGCGGATGGCTACGGCGTCGCGGGTGGCGTCGAGCACGCGGGTGGTCCAGCCTTCCAGGTCCAGTTCACGCTCGTCGGGGTGGGGGCCGTAGTGTAGTTGCCACCAGACCTGGCGTTCCACTTCGCCa                                                                                                       >  6:122201/1‑136 (MQ=255)
                             cccGTTGGCGGATGGCTACGGCGTCGCGGGTGGCGTCGAGCACGCGGGTGGTCCAGCCTTCCAGGTCCAGTTCACGCTCGTCGGGGTGGGGGCCGTAGTGCAGTTGCCACCAGACCTGGCGTTCCACTTCGCCa                                                                                                       <  4:267391/134‑1 (MQ=255)
                                gTTGGCGGATGGCTACGGCGTCGCGGGTGGCGTCGAGCACGCGGGTGGTCCAGCCTTCCAGGTCCAGTTCACGCTCGTCGGGGTGGGGGCCGTAGTGCAGTTGCCACCAGACCTGGCGTTCCACTTCGCCATGGCg                                                                                                  >  4:65753/1‑136 (MQ=255)
                                    gCGGATGGCTACGGCGTCGCGGGGGGCGTCGAGCACGCGGGTGGTCCAGCCTTCCAGGTCCAGTTCACGCTCGTCGGGGTGGGGGCCGTAGTGCAGTTGCCACCAGACCTGGCGTTCCACTTCGCCATGGCGGTCg                                                                                              <  4:159717/136‑1 (MQ=255)
                                          ggCTACGGCGTCGCGGGTGGCGTCGAGCACGCGGGTGGTCCAGCCTTCCAGGTCCAGTTCACGCTCGTCGGGGTGGGGGCCGTAGTGCAGTTGCCACCAGACCTGGCGTTCCACTTCGCCATGGCGGTCGATgcg                                                                                         >  2:273244/1‑135 (MQ=255)
                                           gCTACGGCGTCGCGGGTGGCGTCGAGCACGCGGGTGGTCCAGCCTTCCAGGTCCAGTTCACGCTCGTCGGGGTGGGGGCCGTAGTGCAGTTGCCACCAGACCTGGCGTTCCACTTCGCCATGGCGGTCGATGCGCa                                                                                       <  5:184869/136‑1 (MQ=255)
                                                  cGTCGCGGGTGGCGTCGAGCTCGGGGGTGGTCCAGCCTTCCAGGTCCAGTTCACGCTCGTCGGGGTGGGGGCCGTAGTGCAGTTGCCACCAGACCTGGCGTTCCACTTCGCCATGGCGGTCGATGCGCATCCAGGt                                                                                <  3:203426/136‑1 (MQ=255)
                                                       cgGGTGGCGTCGAGCACGCGGGTGGTCCAGCCTTCCAGGTCCAGTTCACGCTCGTCGGGGTGGGGGCCGTAGTGCAGTTGCCACCAGACCTGGCGTTCCACTTCGCCATGGCGGTCGATGCGCATCCAGGTGCCgg                                                                           >  6:108939/1‑136 (MQ=255)
                                                       cgGGTGGCGTCGAGCACGCGGGTGGTCCAGCCTTCCAGGTCCAGTTCACGCTCGTCGGGGTGGGGGCCGTAGTGCAGTTGCCACCAGACCTGGCGTTCCACTTCGCCATGGCGGTCGATGCGCATCCAGGTGCCgg                                                                           >  8:35804/1‑136 (MQ=255)
                                                        gggTGGCGTCGAGCACGCGGGTGGTCCAGCCTTCCAGGTCCAGTTCACGCTCGTCGGGGTGGGGGCCGTAGTGCAGTTGCCACCAGACCTGGCGTTCCACTTCGCCATGGCGGTCGATGCGCATCCAGGTGCCggg                                                                          <  8:147636/136‑1 (MQ=255)
                                                                 cGAGCACGCGGGTGGTCCAGCCTTCCAGGTCCAGTTCACGCTCGTCGGGGTGGGGGCCGTAGTGCAGTTGCCACCAGACCTGGCGTTCCACTTCGCCATGGCGGTCGATGCGCATCCAGGTGCCGGGCTCCAGCtt                                                                 >  1:209856/1‑136 (MQ=255)
                                                                     cACGCGGGTGGTCCAGCCTTCCAGGTCCAGTTCACGCTCGTCGGGGTGGGGGCCGTAGTGCAGTTGCCACCAGACCTGGCGTTCCACTTCGCCATGGCGGTCGATGCGCATCCAGGTGCCGGGCTCCAGCTTCTGc                                                             <  4:121495/136‑1 (MQ=255)
                                                                      aCGCGGGTGGTCCAGCCTTCCAGGTCCAGTTCACGCTCGTCGGGGTGGGGGCCGTAGTGCAGTTGCCACCAGACCTGGCGTTCCACTTCGCCATGGCGGTCGATGCGCATCCAGGTGCCGGGCTCCAGCTTCTGCa                                                            <  7:242658/136‑1 (MQ=255)
                                                                       cgcgGGTGGTCCAGCCTTCCAGGTCCAGTTCACGCTCGTCGGGGTGGGGGCCGTAGTGTAGTTGCCACCAGACCTGGCGTTCCACTTCGCCATGGCGGTCGATGCGCATCCAGGTGCCGGGCTCCAGCTTCTGCAc                                                           <  5:126905/136‑1 (MQ=255)
                                                                       cgcgGGGGGTCCAGCCTTCCAGGTCCAGTTCACGCTCGTCGGGGTGGGGGCCGTAGTGTAGTTGCCACCAGACCTGGCGTTCCACTTCGCCATGGCGGTCGATGCGCATCCAGGTGCCGGGCTCCAGCTTCTGCAc                                                           <  5:122201/136‑1 (MQ=255)
                                                                                     ccTTCCAGGTCCAGTTCACGCTCGTCGGGGTGGGGGCCGTAGTGCAGTTGCCACCAGACCTGGCGTTCCACTTCGCCATGGCGGTCGATGCGCATCCAGGTGCCGGGCTCCAGCTTCTGCACGTTGGCCAGCAggg                                             >  2:174430/1‑136 (MQ=255)
                                                                                         ccAGGTCCAGTTCACGCTCGTCGGGGTGGGGGCCGTAGTGCAGTTGCCACCAGACCTGGCGTTCCACTTCGCCATGGCGGTCGATGCGCATCCAGGTGCCGGGCTCCAGCTTCTGCACGTTGGCCAGCAGGGTg                                           <  8:101651/134‑1 (MQ=255)
                                                                                         ccAGGTCCAGTTCACGCTCGTCGGGGTGGGGGCCGTAGTGCAGTAGCCACCAGACCTGGCGTTCCACTTCGCCATGGCGGTCGATGCGCATCCAGGTGCCGGGCTCCAGCTTCTGCACGTTGGCCAGCAGGGGgc                                          <  6:225452/135‑1 (MQ=255)
                                                                                         ccAGGTCCAGTTCACGCTCGGCGGGGTGGGGGCCGTAGTGCAGTTGCCACCAGACCTGGCGTTCCACTTCGCCATGGCGGTCGATGCGCATCCAGGTGCCGGGCTCCAGCTTCTGCACGTTGGCCAGCAGGGTg                                           <  7:113717/134‑1 (MQ=255)
                                                                                           aGGTCCAGTTCACGCTCGGCGGGGTGGGGGCCGTAGTGCAGTTGCCACCAGACCTGGCGTTCCACTTCGCCATGGCGGTCGATGCGCATCCAGGTGCCGGGCTCCAGCTTCTGCACGTTGGCCAGCAGGGTGcgcg                                       <  3:128306/136‑1 (MQ=255)
                                                                                                           cgtcgGGGTGGGGGCCGTAGTGCAGTTGCCACCAGACCTGGCGTTCCACTTCGCCATGGCGGTCGATGCGCATCCAGGTGCCGGGCTCCAGCTTCTGCACGTTGGCCAGCAGGGTGCGCGGTGCCGGTACCACGGc                       >  3:125993/1‑136 (MQ=255)
                                                                                                                gggTGGGGGCCGTAGTGCAGTTGCCACCAGACCTGGCGTTCCACTTCGCCATGGCGGTCGATGCGCATCCAGGTGCCGGGCTCCAGCTTCTGCACGTTGGCCAGCAGGGTGCGCGGTGCCGGTACCACGGCGTgg                   >  2:162616/1‑135 (MQ=255)
                                                                                                                 ggTGGGGGCCGTAGTGCAGTTGCCACCAGACCTGGCGTTCCACTTCGCCATGGCGGTCGATGCGCATCCAGGTGCCGGGCTCCAGCTTCTGCACGTTGGCCAGCAGGGTGCGCGGTGCCGGTACCACGGCGTGGaa                 <  7:79799/136‑1 (MQ=255)
                                                                                                                   tGGGGGCCGTAGTGCAGTTGCCACCAGACCTGGCGTTCCACTTCGCCATGGCGGTCGATGCGCATCCAGGTGCCGGGCTCCAGCTTCTGCACGTTGGCCAGCAGGGTGCGCGGTGCCGGTACCACGGCGTGGAAAt               <  4:64939/136‑1 (MQ=255)
                                                                                                                    gtgtgCCGTAGTGCAGTTGCCACCAGACCTGGCGTT‑CACTTCGCCATGGCGGTCGATGCGCATCCAGGTGCCGGGCTCCAGCTTCTGCACGTTGGCCAGCAGGGTGCGCGGTGCAGGTACCACGGCGTGGAAATTc             <  2:154540/132‑1 (MQ=255)
                                                                                                                        gCCGTAGTGCAGTTGCCACCAGACCTGGCGTTCCACTTCGCCATGGCGGTCGATGCGCATCCAGGTGCCGGGCTCCAGCTTCTGCACGTTGGCCAGCAGGGTGCGCGGTGCCGGTACCACGGCGTGGAAATTCAgg          <  2:184914/136‑1 (MQ=255)
                                                                                                                         ccGTAGTGCAGTTGCCACCAGACCTGGCGTTCCACTTCGCCATGGCGGTCGAAGCGCATCCAGGTGCCGGGCTCCAGCTTCTGCACGTTGGCCAGCAGGGTGCGCGGTGCCGGTACCACGGCGTGGAAATTCAGGt         >  6:164595/1‑136 (MQ=255)
                                                                                                                              gTGCAGTTGCCACCAGACCTGGCGTTCCACTTCGCCATGGCGGTCGATGCGCATCCAGGTGCCGGGCTCCAGCTTCTGCACGTTGGCCAGCAGGGCGCGCGGTGCCGGTACCACGGCGTGGAAATTCAGGTAGTgg    >  6:122563/1‑136 (MQ=255)
                                                                                                                               tGCAGTTGCCACCAGACCTGGCGTTCCACTTAGACATGTCGGTCGATGCGCATCCAGGTGCCGGGCTCCAGCTTCTGCACGTTGGCCAGCAGGGTGCGCGGTGCCGGTACCAGGGCGTGGAAAATCAGGGAGTggg   >  2:127893/1‑135 (MQ=255)
                                                                                                                                gCAGTTGCCACCAGACCTGGCGTTCCACTTCGCCATGGCGGTCGATGCGCATCCAGGTGCCGGGCTCCAGCTTCTGCACGTTGGCCAGCAGGGTGCGCGGTGCCGGTACCACGGCGTGGAAATTCAGGTAGTGGtt  >  8:11994/1‑136 (MQ=255)
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GGACAGCAACACACCCACATCGACGGCTGCCCGTTGGCGGATGGCTACGGCGTCGCGGGTGGCGTCGAGCACGCGGGTGGTCCAGCCTTCCAGGTCCAGTTCACGCTCGTCGGGGTGGGGGCCGTAGTGCAGTTGCCACCAGACCTGGCGTTCCACTTCGCCATGGCGGTCGATGCGCATCCAGGTGCCGGGCTCCAGCTTCTGCACGTTGGCCAGCAGGGTGCGCGGTGCCGGTACCACGGCGTGGAAATTCAGGTAGTGGTT  >  ACB143/1943684‑1943947

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑