Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB143 3,317,589 C→T 8.1% G185G (GGG→GGA)  CJ019_03118 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1433,317,5890CT 8.1% 150.4 / 4.6 51G185G (GGG→GGA) CJ019_03118hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (21/25);  new base T (2/2);  total (23/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.06e-01

CGTCGGTGCTGTCGGCGCCGACGATGACTTGGGTGCTCTGCCCGGCCGGGGTAAAGCGCGGCGCCCTGGGCTCGTTGGCCACGGCCTGCTGGCCCTGATGAATCACGCCCATGGCCTGGCGAATGGTGT‑GCCCTTGCTTTTCTGGCGCCAGGCGCTTGAGGCTGGCGTCGGTGGGCAGTTCGATGTTGACGCTGAGGCGGTCGGCCAGGCGCCCGGCCTCTTCTATCAGCAGCGGGTCGGCATCGGGGATGGCCTTGAGGTG  >  ACB143/3317459‑3317720
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cgtcgGTGCTGTCGGCGCCGACGATGACTTGGGTGCTCTGCCCGGCCGGGGTAAAGCGCGGCGCCCTGGGCTCGTTGGCCACGGCCTGCTGGCCCTGATGAATCCCGCCCATGGCCTGGCGAATGGTGT‑GCCCTTg                                                                                                                                >  1:180485/1‑136 (MQ=255)
  tcgGTGCTGTCGGCGCCGACGATGACTTGGGTGCTCTGCCCGGCCGGGGTAAAGCGCGGCGCCCTGGGCTCGTTGGCCACGGCCTGCTGGCCCTGATGAATCACGCCCATGGCCTGGCGAATGGTGT‑GCCCTTGCt                                                                                                                              >  8:126384/1‑136 (MQ=255)
  tcgGTGCTGTCGGCGCCGACGATGACTTGGGTGCTCTGCCCGGCCGGGGTAAAGCGCGGCGCCCTGGGCTCGTTGGCCACGGCCTGCTGGCCCTGATGAATCACGCCCATGGCCTGGCGAATGGTGT‑GCCCTTGCt                                                                                                                              >  4:145643/1‑136 (MQ=255)
  tcgGTGCTGTCGGCGCCGACGATGACTTGGGTGCTCTGCCCGGCCGGGGTAAAGCGCGGCGCCCTGGGCTCGTTGGCCACGGCCTGCTGGCCCGGATGAATCACGCCCATGGCCTGGCGAATGGGGT‑GCCCGTGCt                                                                                                                              >  1:118684/1‑136 (MQ=255)
       gCTGTCGGCGCCGACGATGCCTTGGGTGCTCTGCCCGGCCGGGGTAAAGCGCGGCGCCCTGGGCTCGTTGGCCCCGGCCTGCTGGCCCTGATGAATCACGCCCATGGCCTGGCGAATGGTGT‑GCCCTTGCTTTTCt                                                                                                                         >  2:254735/1‑136 (MQ=255)
       gCTGTCGGCGCCGACGATGACTTGGGTGCTCTGCCCGGCCGGGGTAAAGCGCGGCGCCCTGGGCTCGTTGGCCCCGGCCTGCTGGCCCTGATGAATAACTCCCATGGCCTGGCGAATGGTGT‑GCCCTGGCTTTTCt                                                                                                                         >  8:108723/1‑136 (MQ=255)
       gCTGTCGGCGCCGACGATGACTTGGGTGCTCTGCCCGGCCGGGGTAAAGCGCGGCGCCCTGGGCTCGTTGGCCACGGCCTGCTGGCCCTGATGAATCACGCCCATGGCCTGGCGAATGGTGT‑GCCCTTGCTTTTCt                                                                                                                         >  5:83773/1‑136 (MQ=255)
       gCTGTCGGCGCCGACGATGACTTGGGTGCTCTGCCCGGCCGGGGTAAAGCGCGGCGCCCTGGGCTCGTTGGCCACGGCCTGCTGGCCCTGATGAATCACGCCCATGGCCTGGCGAATGGTGT‑GCCCTTGCTTTTCt                                                                                                                         >  5:287647/1‑136 (MQ=255)
       gCTGTCGGCGCCGACGATGACTTGGGTGCTCTGCCCGGCCGGGGTAAAGCGCGGCCCCCTGGGCTCGTTGGCCACGGCCTGCTGGCCCTGATGAATCACGCCCATGGCCTGGCGAATGGTGT‑GTCCTTGCTTTTCt                                                                                                                         >  4:227321/1‑136 (MQ=255)
       gCTGTCGGCGCCGACGATGACTTGGGTGCTCTGCCCGGCCGGGGTAAAGCGCGGCCCCCTGGGCTCGTTGGCCACGGCCTGCTGGCCCTGATGAATCACGCCCATGGCCTGGCGAATGGTGT‑GTCCTTGCTTTTCt                                                                                                                         >  4:223298/1‑136 (MQ=255)
       gCTGTCGGCGCCCACGATGACTTTGGTGCTCTTCCCGGCCGGGGTAAAGCGCCGCCCCCTGGGCTCGATGGCCACCGCCAGCTGGCCCTGATTAAACACCCCCAAGGGCGGGGGAAATGTGTGGCCCTTGttttaa                                                                                                                          >  2:152547/1‑134 (MQ=255)
            cGGCGCCGACGATGACTTGGGTGCTCTGCCCGGCCGGGGTAAAGCGCGGCGCCCTGGGCTCGTTGGCCACGGCCTGCTGGCCCTGATGAATCACGCCCATTGCCTGGCGAATGGTGT‑GCCCTTGCTTTTCTGgcgc                                                                                                                    >  7:171905/1‑136 (MQ=255)
              gcgcCGACGATGACTTGGGTGCTCTGCCCGGCCGGGGTAAAGCGCGGCGCCCTGGGCTCGTTGGCCACGGCCTGCTGGCCCTGATGCATCACGCACATGGCCTGGCGAATGGTGT‑GCCAGTGCTTTTCTGgcgc                                                                                                                    >  4:230112/1‑134 (MQ=255)
                gcCGACGATGACTTGGGTGCTCTGCCCGGCCGGGGTAAAGCGCGGCGCCCTGGGCTCGTTGGCCACGGCCTGCTGGCCCTGATGAATCACGCCCATGGCCTGGCGAATGGTGT‑GCCCTTGCTTTTCTGGCGCCAgg                                                                                                                >  3:206621/1‑136 (MQ=255)
                   gacgaTGACTTGGGTGCTCTGCCCGGCCGGGGTAAAGCGCGGCGCCCTGGGCTCGTTGGCCACGGCCTGCTCGCCCTGATGAATCACGCCCATGGCCTGGCGAATGGTGT‑GCCCTTGCTTTTCTGGCGCCAGgcgc                                                                                                             >  2:175823/1‑136 (MQ=255)
                      gaTGACTTGGGTGCTCTGCCAGGCCGGGGTAAAGCGCGGCGCACTGGGCTCGTTGGCCACGTCCTGCTGGCCCTGATGAATCACGCCCATGGCCTTGCGAATGGATG‑GCCCTTGCTTTGCTGGCGCCAGGCGCtt                                                                                                           >  8:135789/1‑135 (MQ=255)
                          aCTTGGGTGCTCTGCCCGGCCGGGGTAAAGCGCGGCGCCCTGGGCTCGTTGGCCACGGCCTGCTGGCCCTGATGAATCACGCCCATGGCCTGGCGAATGGTGT‑GCCCTTGCTTTTCTGGCGCCAGGCGCTTGAGGc                                                                                                      <  3:39207/136‑1 (MQ=255)
                                    gctGCCCGGGCGGGGGAAAGCGCGGCGCCCTGGGCTCGGTGGCCACGGCCTGCTGGCCCTGATGAATCACGCCCATGGCCTGGCGAATGGTGG‑GCCCTTGCTTTTCTGGCGCCAGGCGCTTGAGGCTGGCGTCGGt                                                                                            <  6:225045/135‑1 (MQ=255)
                                    gctGCCCGGCCGGGGTAAAGCGCGGCGCCCTGGGCTCGTTGGCCACGGCCTGCTGGCCCTGATGAATCACGCCCATGGCCTGGCGAATGGTGT‑GCCCTTGCTTTTCTGGCGCCAGGCGCTTGAGGCTGGCGTCGGt                                                                                            <  1:152547/135‑1 (MQ=255)
                                      tGCCCGGCCGGGGTAAAGCGCGGCGCCCTGGGCTCGTTGGCCACGGCCTGCTGGCCCTGATGAATCACGCCCATGGCCTGGCGAATGGTGT‑GCCCTTGCTTTTCTGGCGCCAGGCGCTTGAGGCTGGCGTCGGt                                                                                            <  3:20049/134‑1 (MQ=255)
                                      tGCCCGGCCGGGGTAAAGCGCGGCCCCCTGGGCTCGTTGGCCACGGCCTGCTGGCCCTGATGAATCACGCCCATGGCCTGGCGAATGGTGT‑GTCCTTGCTTTTCTGGCGCCAGGCGCTTGAGGCTGGCGTCGGt                                                                                            <  3:223298/134‑1 (MQ=255)
                                      tGCCCGGCCGGGGTAAAGCGCGGCCCCCTGGGCTCGTTGGCCACGGCCTGCTGGCCCTGATGAATCACGCCCATGGCCTGGCGAATGGTGT‑GTCCTTGCTTTTCTGGCGCCAGGCGCTTGAGGCTGGCGTCGGt                                                                                            <  3:227321/134‑1 (MQ=255)
                                                gggTAAAGCGCGGCGCCCTGGGCTCGTTGGCCACGGCCTGCTGGCCCTGATGAATCACGCCCATGGCCTGGCGAATGGTGT‑GCCCTTGCTTTTCTGGCGCCAGGCGCTTGAGGCTGGCGTCGGTGGGCAGTTCGAt                                                                                <  3:78153/136‑1 (MQ=255)
                                                                           tGGCCACGGCCTGCTGGCCCTGATGAATCACGCCCATGGCCTGGCGAATGGTGT‑GCCCTTGCTTTTCTGGCGCCAGGCGCTTGAGGCGGGCGTCGGTGGGCAGTTCGATGTTGACGCTGAGGCGGTCGGCCAGgcg                                                     <  6:137965/136‑1 (MQ=255)
                                                                             gcgtcGGCCTGCTGGCCCTGATGAATCACGCCCATGGCCTGGCGAATGGTGT‑GCCCTTGCTTTTCTGGCGCCAGGCGCTTGAGGCTGGCGTCGGTGGGCAGTTCGATGTTGACGCTGAGGCGGTCGGCCAGGCGcc                                                   <  4:37239/132‑1 (MQ=255)
                                                                             gCCACGGCCTGCTGGCCCTGATGAATCCCGCCCATGGCCTGGCGAATGGTGT‑GCCCTTGCTTTTCTGGCGCCAGGCGCTTGAGGCTGGCGTCGGTGGGCAGTTCGATGTTGACGCTGAGGCGGTCGGCCAGGCGcc                                                   <  2:248323/136‑1 (MQ=255)
                                                                             gCCACGGCCTGCTGGCCCTGATGAATCACGCCCATGGCCTGGCGAATGGTGT‑GCCCTTGCTTTTCTGGCGCCAGGCGCTTGAGGCTGGCGTCGGTGGGCAGTTCGATGTTGACGCTGAGGCGGTCGGCCAGGCGcc                                                   <  3:72251/136‑1 (MQ=255)
                                                                              ccACGGCCTGCTGGCCCTGATGAATCACGCCCATGGCCTGGCGAATGGTGT‑GCCCTTGCTTTTCTGGCGCCAGGCGCTTGAGGCTGGCGTCGGTGGGCAGTTCGATGTTGACGCTGAGGCGGTCGGCCAGGCGccc                                                  <  6:64903/136‑1 (MQ=255)
                                                                                  ggCCTGCTGGCCCTGATGAATCACGCCCATGGCCTGGCGAATGGTGT‑GCCCTTGCTTTTCTGGCGCCAGGCGCTTGAGGCTGGCGTCGGTGGGCAGTTCGATGTTGACGCTGAGGCGGTCGGCCAGGCGCCCGGcc                                              <  2:23799/136‑1 (MQ=255)
                                                                                           gCCCTGATGAATCACGCCCATGGCCTGGCGAATGGTGT‑GCCCTTGCTTTTCTGGCGCCAGGCGCTTGAGGCTGGCGTCGGTGGGCAGTTCGATGTTGACGCTGAGGCGGTCGGCCAGGCGCCCGGCCTCTTCTAt                                      >  5:247199/1‑135 (MQ=255)
                                                                                                  ggaATCACGCCCATGGCCTGGCGAATGGTGT‑GCCCTTGCTTTTCTGGCGCCAGGCGCTTGAGGCTGGCGTCGGTGGGCAGTTCGATGTTGACGCTGAGGCGGTCGGCCAGGCGCCCGGCCTCTTCTATCATCAGCg                              <  1:295116/135‑1 (MQ=255)
                                                                                                        aCGCCCATGGCCTGGCGAATGGTGT‑GCCCTTGCTTTTCTGGCGCCAGGCGCTTGAGGCTGGCGTCGGTGGGCAGTTCGATGTTGACGCTGAGGCGGTCGGCCAGGCGCCCGGCCTCGTCTATCAGCAGCGGGTCgg                        >  8:207578/1‑136 (MQ=255)
                                                                                                            ccATGGCCTGGCGAAGGGTGT‑GCCCGTGCTTTTCTGGCGCCAGGCGCTTGAGGCTGGCGTCGGTGGGCAGTTCGATGTTGACGCTGAGGCGGTCGGCCAGGCGCCCGGCCTCTTCTATCAGCAGCGGGTCGGCATc                    <  6:71591/136‑1 (MQ=255)
                                                                                                              aTGGGCTGGCGAATGGTGT‑GCCCTTGCTTTTCTGGCGCCAGGCGCTTGAGGCTGGCGTCGGTGGGCAGTTCGATGTTGACGCTGAGGCGGTCGGCCAGGCGCCCGGCCTCTTCTATCAGCAGCGGGTCGGCATCgg                  <  6:250478/136‑1 (MQ=255)
                                                                                                              aTGGCCTGGCGAATGTTGT‑GCCCTTGCTTTTCTGGCGCCAGGCGCTTGAGGCTGGCGTCGGTGGGCAGTTCGATGTTGACGCTGAGGCGGTCGGCCAGGCGCCCGGCCTCTTCTATCAGCAGCGGGTCGGCATCgg                  <  1:98871/136‑1 (MQ=255)
                                                                                                              aTGGCCTGGCGAATGGTGT‑GCCCTTGCTTTTCTTGCGCCAGGCGCTTGAGGCTGGCGTCGGTGGGCAGTTCGATGTTGACGCTGAGGCGGTCGGCCAGGCGCCCGGCCTCTTCTATCAGCAGCGGGTCGGCATCgg                  <  3:104403/136‑1 (MQ=255)
                                                                                                              aTGGCCTGGCGAATGGTGT‑GCCCTTGCTTTTCTGGCGCCAGGCGCTTGAGGCTGGCGTCGGTGGGCAGTTCGATGTTGACGGTGAGGCGGTCGGCCAGGCGCCCGGCCTCTTCTATCAGCAGCGGGTCGGCATCgg                  <  8:130568/136‑1 (MQ=255)
                                                                                                              aTGGCCTGGCGAATGGTGT‑GCCCTTGCTTTTCTGGCGCCAGGCGCTTGAGGCTGGCGTCGGTGGGCAGTTCGATGTTGACGCTGAGGCGGTCGGCCAGGCGCCCGGCCTCTTCTATCAGCAGCGGGTCGGCATCgg                  <  2:33130/136‑1 (MQ=255)
                                                                                                              aTGGCCTGGCGAATGGTGT‑GCCCTTGCTTTTCTGGCGCCAGGCGCTTGAGGCTGGCGTCGGTGGGCAGTTCGATGTTGACGCTGAGGCGGTCGGCCAGGCGCCCGGCCTCTTCTATCAGCAGCGGGTCGGCATCgg                  <  7:179916/136‑1 (MQ=255)
                                                                                                              aTGGCCTGGCGAATGGTGT‑GCCCTTGCTTTTCTGGCGCCAGGCGCTTGAGGCTGGCGTCGGTGGGCAGTTCGATGTTGACGCTGAGGCGGTCGGCCAGGCGCCCGGCCTCTTCTATCAGCAGCGGGTCGGCATCgg                  <  3:120402/136‑1 (MQ=255)
                                                                                                                 gCCTGGCGAATGGTGT‑GCCCTTGCTTTTCTGGCGCCAGGCGCTTGAGGCTGGCGTCGGTGGGCAGTTCGATGTTGACGCTGAGGCGGTCGGCCAGGCGCCCgg                                                >  1:78118/1‑103 (MQ=255)
                                                                                                                 gCCTGGCGAATGGTGT‑GCCCTTGCTTTTCTGGCGCCAGGCGCTTGAGGCTGGCGTCGGTGGGCAGTTCGATGTTGACGCTGAGGCGGTCGGCCAGGCGCCCgg                                                <  2:78118/103‑1 (MQ=255)
                                                                                                                      gCGAATGGTGT‑GGCCTTGCTTTTCTGGCGCCTGGCGCTTGAGGCTTGCGTCGGTGGGCAGTTCGATGTTGACGCTGAGGCGGTCGGCCAGGCGCCCGGCCTCTTCTATCAGCAGCGGGTCGGCATCGGGGATGGc           <  2:128301/135‑1 (MQ=255)
                                                                                                                      gCGAATGGTGT‑GCCCTTGCTTTTCTGGCGCCAGGCGCTTGAGGCTGGCGTCGGTGGGCAGTTCGATGTTGACGCTGAGGCGGTCGGCCAGGCGCCCGGCCTCTTCTATCAGCAGCGGGTCGGCATCGGGGATgg            <  5:283648/134‑1 (MQ=255)
                                                                                                                      gCGAATGGTGT‑GCCCTTGCTTTTCTGGCGCCAGGCGCTTGAGGCTGGCGTCGGTGGGCAGTTCGATGTTGACGCTGAGGCGGTCGGCCAGGCGCCCGGCCTCTTCTATCAGCAGCGGGTCGGCATCGGGGATgg            <  5:120350/134‑1 (MQ=255)
                                                                                                                      gCGAATGGTGT‑GCCCTTGCTTTTCTGGCGCCAGGCGCTTGAGGCTGGCGTCGGTGGGCAGTTCGATGTTGACGCTGAGGCGGTCGGCCAGGCGCCCGGCCTCTTCTATCAGCAGCGGGTCGGCATCGGGGATGGc           <  3:142405/135‑1 (MQ=255)
                                                                                                                      gCGAATGGTGT‑GCCCTTGCTTTTCTGGCGCCAGGCGCTTGAGGCTGGCGTCGGTGGGCAGTTCGATGTTGACGCTGAGGCGGTCGGCCAGGCGCCCGGCCTCTTCTATCAGCAGCGGGTCGGCATCGGGGATGGc           <  3:223499/135‑1 (MQ=255)
                                                                                                                         aaTGGTGT‑GCCCTTGCTTTTCTGGCGCCAGGCGCTTGAGGCTGGCGTCGGTGGGCAGTTCGATGTTGACGCTGAGGCGGTCGGCCAGGCGCCCGGCCTCTTCTATCAGCAGCGGGTCGGCATCGGGGATGGCCTTg       >  5:211776/1‑136 (MQ=255)
                                                                                                                         aaTGGTGT‑GCCCTTGCTTTTCTGGCGCCAGGCGCTTGAGGCTGGCGGCGGTGGGCAGTTCGATGTTGACGCTGAGGCGGTCGGCCCGGCGCCCGGCCTCGTCTATCAGCAGCGGGTCGGCCTCGGGGATGGCCtgg       >  4:102055/1‑134 (MQ=255)
                                                                                                                              tgt‑gCCCTTGCTTTTCTGGCGCCAGGCGCTTGAGGCTGGCGTCGGTGGGCAGTTCGATGTTGACGCTGAGGCGGTCGGCCAGGCGCCCGGCCTCTTCTATCAGCAGCGGGTCGGCATCGGGGATGGCCTTGAGGTg  >  4:180807/1‑136 (MQ=255)
                                                                                                                              tgt‑gCCCTTGCTTTTCTGGCGCCAGGCGCTTGAGGCTGGCGTCGGTGGGCAGTTCGATGTTGACGCTGAGGCGGTCGGCCAGGCGCCCGGCCTCTTCTATCAGCAGCGGGTCGGCATCGGGGATGGCCGTGAGGTg  >  4:177317/1‑136 (MQ=255)
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CGTCGGTGCTGTCGGCGCCGACGATGACTTGGGTGCTCTGCCCGGCCGGGGTAAAGCGCGGCGCCCTGGGCTCGTTGGCCACGGCCTGCTGGCCCTGATGAATCACGCCCATGGCCTGGCGAATGGTGT‑GCCCTTGCTTTTCTGGCGCCAGGCGCTTGAGGCTGGCGTCGGTGGGCAGTTCGATGTTGACGCTGAGGCGGTCGGCCAGGCGCCCGGCCTCTTCTATCAGCAGCGGGTCGGCATCGGGGATGGCCTTGAGGTG  >  ACB143/3317459‑3317720

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑