Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB143 5,087,838 C→T 5.8% P229P (CCG→CCA)  occM_3 ← Octopine transport system permease protein OccM

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1435,087,8380CT 5.8% 228.9 / 2.2 70P229P (CCG→CCA) occM_3Octopine transport system permease protein OccM
Reads supporting (aligned to +/- strand):  ref base C (27/38);  new base T (2/2);  total (29/41)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.65e-01

TGCTGATGACGTCGCCGGCCTTGGCCGCCAGCGACACGCCCTTGAGCACTTCGTGGCTGCCGTAGCGCTTGTGCAGGTCTTGGACTTCGAGTTTGTACATGCTGTCGATTCTCACAAAGCGGTCAGTGCTTGCGCGGCGCCAGGTAGCCGAGCCAGCGGCGTTCGGCCATCTTGAACAGGCGCACCAGGATGAAGGTCAGGCACAGGTAGAACACGCCCGCCGTGATATAGGCCTCGAAAGGCAGGTAGTACTGGGCATTGACCGTGC  >  ACB143/5087704‑5087971
                                                                                                                                      |                                                                                                                                     
tgCTGATGACGTCGCCGGCCTTGGCCGCCAGCGACACGCCCTTGAGCACTTCGTGGCTGCCGTAGCGCTTGTGCAGGTCTTGGACTTCGAGTTTGTACATGCTGTCGATTCTCACAAAGCGGTCAGTGCTTGcgcg                                                                                                                                      >  2:165232/1‑136 (MQ=255)
        aCGTCGCCGGCCTTGGCCGCCAGCGACACGCCCTTGAGCACTTCGTGGCTGCCGTAGCGCTTGTGCAGGTCTTGGACTTCGAGTTTGTACATGCTGTCGATTCTCCCAAAGCGGTCAGTGCTTGCGCGGCGCCAgg                                                                                                                              >  4:153840/1‑136 (MQ=255)
        aCGTCGCCGGCCTTGGCCGCCAGCGACACGCCCTTGAGCACTTCGTGGCTGCCGTAGCGCTTGTGCAGGTCTTGGACTTCGAGTTTGTACATGCTGTCGATTCTCACAAAGCGGTCAGTGCTTGCGTGGCGCCAgg                                                                                                                              >  1:141919/1‑136 (MQ=255)
        aCGTCGCCGGCCTTGGCCGCCAGCGACACGCCCTTGAGCACTTCGTGGCTGCCGTAGCGCTTGTGCAGGTCTTGGACTTCGAGTTTGTACATGCTGTCGATTCTCACAAAGCGGTCAGTGCTTGCGTGGCGCCAgg                                                                                                                              >  1:137883/1‑136 (MQ=255)
         cGTCGCCGGCCTTGGCCGCCAGCGACACGCCCTTGAGCACTTCGTGGCTGCCGTAGCGGTTGTGCAGGTCTTGGACTTCGAGTTTGTTCATGCTGTCGATTCTCACAAAGCGGTCAGTGCTTGCGCGGCGCCAGGt                                                                                                                             <  1:97906/136‑1 (MQ=255)
         cGTCGCCGGCCTTGGCCGCCAGCGACACGCCCTTGAGCACTTCGTGGCTGCCGTAGCGCTTGTGCAGGTCTTGGACTTCGAGTTTGTACATGCTGTCGATTCTCACAAAGCGGTCAGTGCTTGCGCGGCGCCAGGt                                                                                                                             <  1:251508/136‑1 (MQ=255)
          ggcGCCGGCCTTGGCCGCCAGCGACACGCCCTTGAGCACTTCGTGGCTGCCGTAGCGCTTGTGCAGGTCTTGGACTTCGAGTTTGTACATGCTGTCGATTCTCACAAAGCGGTCAGTGCTTGCGCGGCGCCAGGTa                                                                                                                            <  3:209674/134‑1 (MQ=255)
             gCCGGCCTTGGCCGCCAGCGACACGCCCTTGAGCACTTCGTGGCTGCCGTAGCGCTTGTGCAGGTCTTGGACTTCGAGTTTGTACATGCTGTCGATTCTCACAAAGCGGTCAGTGCTTGCGCGGCGCCAGGTAGcc                                                                                                                         >  8:89272/1‑136 (MQ=255)
               cGGCCTTGGCCGCCAGCGACACGCCCTTGAGCACTTCGTGGCTGCCGTAGCGCTTGTGCAGGTCTTGGACTTCGAGTTTGTACATGCTGTCGATTCTCACAAAGCGGTCAGTGCTTGCGCGGCGCCAGGTAGcc                                                                                                                         >  4:86941/1‑134 (MQ=255)
               cGGCCTTGGCCGCCAGCGACACGCCCTTGAGCACTTCGTGGCTGCCGTAGCGCTTGTGCAGGTCTTGGACTTCGAGTTTGTACATGCTGTCGATTCTCACAAAGCGGTCAGTGCTTGCGCGGCGCCAGGTAGcc                                                                                                                         >  3:90825/1‑134 (MQ=255)
               cGGCCTTGGCCGCCAGCGACACGCCCTTGAGCACTTCGTGGCTGCCGTAGCGCTTGTGCAGGTCTTGGACTTCGAGTTTGTACATGCTGTCGATTCTCACAAAGCGGTCAGTGCTTGCGCGGCGCCAGGTAGcc                                                                                                                         >  8:206247/1‑134 (MQ=255)
                           ccAGCGACACGCCCTTGAGCACTTCGTGGCTGCCGTAGCGCTTGTGCAGGTCTTGGACTTCGAGTTTGTACATGCTGTCGATTCTCACAAAGCGGTCAGTGCTTGCGCGGCGCCAGGTAGCCGAGCCAGCGGCGtt                                                                                                           <  5:134001/136‑1 (MQ=255)
                                           gAGCACTTCGTGGCTGCCGTAGCGCTTGTGCAGGTCTTGGACTTCGAGTTTGTACATGCTGTCTATTCTCACAAAGCGGTCAGTGCTTGCGCGGCGCCAGGTAGCCGAGCCAGCGGCGTTCGGCCATCTTGAAc                                                                                             <  6:45141/134‑1 (MQ=255)
                                            aGCACTTCGTGGCTGCCGTAGCGCTTGTGCAGGTCTTGGACTTCGAGTTTGTACATGCTGTCGATTCTCACAAAGCGGTCAGTGCTTGCGCGGCGCCAGGTAGCCGAGCCAGCGGCGTTCGGCCATCTTGAACAg                                                                                           <  1:5988/135‑1 (MQ=255)
                                                    gTGGCTGCCGTAGCGCTTGTGCAGGTCTTGGACTTCGAGTTTGTACATGCTGTCGATTCTCACAAAGCGGTCAGTGCTTGCGCGGCGCCAGGTAGCCGAGCCAGCGGCGTTCGGCCATCTTGAACAGGCGCACCAg                                                                                  >  5:98448/1‑136 (MQ=255)
                                                    gTGGCTGCCGTAGCGCTTGTGCAGGTCTTGGACTTCGAGTTTGTACATGCTGTCGATTCTCACAAAGCGGTCAGTGCTTGCGCGGCGCCAGGTAGCCGAGCCAGCGGCGTTCGGCCATCTTGAACAGGCGCACCAg                                                                                  <  4:277330/136‑1 (MQ=255)
                                                    gTGGCTGCCGTAGCGCTTGTGCAGGTCTTGGACTTCGAGTTTGTACATGCTGTCGATTCTCACAAAGCGGTCAGTGCTTGCGCGGCGCCAGGTAGCCGAGCCAGCGGCGTTCGGCCATCTTGAACAGGCGCACCAg                                                                                  >  3:250651/1‑136 (MQ=255)
                                                    gTGGCTGCCGTAGCGCTTGTGCAGGTCTTGGACTTCGAGTTTGTACATGCTGTCGATTCTCACAAAGCGGTCAGTGCTTGCGCGGCGCCAGGTAGCCGAGCCAGCGGCGTTCGGCCATCTTGAACAGGCGCACCAg                                                                                  >  3:139769/1‑136 (MQ=255)
                                                      ggCTGCGGTAGCGCTTGTGCAGGTCTTGGACTTCGAGTTTGTACATGCTGTCGATTCTCACAAAGCGGTCAGTGCTTGCGCGGCGCCAGGTAGCCGAGCCAGCGGCGTTCGGCCATCTTGAACAGGCGCACCAGGa                                                                                <  4:222395/136‑1 (MQ=255)
                                                      ggCTGCCGTAGCGCTTGTGCAGGTCTTGGACTTGGAGTTTGTACATGCTGTCGATTCTCAGAAAGCGGTCAGTGCTTGCGGGGCGCGAGGTAGCCGAGCGAGCGGCGTTCGGCCATCTTGAACAGGCGCACCAGGa                                                                                <  2:297800/136‑1 (MQ=255)
                                                      ggCTGCCGTAGCGCTTGTGCAGGTCTTGGACTTCGAGTTTGTACATGCTGTCGATTCTCACAAAGCGGTCAGTGCTTGCGCGGCGCCAGGTAGCCGAGCCAGCGGCGTTCGGCCATCTTGAACAGGCGCACCAgg                                                                                 >  5:163748/1‑135 (MQ=255)
                                                      ggCTGCCGTAGCGCTTGTGCAGGTCTTGGACTTCGAGTTTGTACATGCTGTCGATTCTCACAAAGCGGTCAGTGCTTGCGCGGCGCCAGGTAGCCGAGCCAGCGGCGTTCGGCCATCTTGAACAGGCGCACCAGGa                                                                                >  5:85307/1‑136 (MQ=255)
                                                      ggCTGCCGTAGCGCTTGTGCAGGTCTTGGACTTCGAGTTTGTACATGCTGTCGATTCTCACAAAGCGGTCAGTGCTTGCGCGGCGCCAGGTAGCCGAGCCAGCGGCGTTCGGCCATCTTGAACAGGCGCACCAGGa                                                                                >  4:233641/1‑136 (MQ=255)
                                                      ggCTGCCGTAGCGCTTGTGCAGGTCTTGGACTTCGAGTTTGTACATGCTGTCGATTCTCACAAAGCGGTCAGTGCTTGCGCGGCGCCAGGTAGCCGAGCCAGCGGCGTTCGGCCATCTTGAACAGGCGCACCAGGa                                                                                >  7:240582/1‑136 (MQ=255)
                                                      ggCTGCCGTAGCGCTTGTGCAGGTCTTGGACTTCGAGTTTGTACATGCTGTCGATTCTCACAAAGCGGTCAGTGCTTGCGCGGCGCCAGGTAGCCGAGCCAGCGGCGTTCGGCCATCTTGAACAGGCGCACCAGGa                                                                                >  4:13517/1‑136 (MQ=255)
                                                         tGCCGTAGCGCTTGTGCAGGTCTTGGACTTCGAGTTTGTACATGCTGTCGATTCTCACAAAGCGGTCAGTGCTTGCGCGGCGCCAGGTAGCCGAGCCAGCGGCGTTCGGCCATCTTGAACAGGCGCACCAGGATg                                                                              >  2:218105/1‑135 (MQ=255)
                                                           ccGTAGCGCTTGTGCAGGTCTTGGACTTCGAGTTTGTACATGCTGTCGATTCTCACAAAGCGGTCAGTGCTTGCGCGGCGCCAGGTAGACGAGCCAGCGGCGTTCGGCCATCTTGAACAGGCGCACCAGGATCaa                                                                            <  7:270992/135‑1 (MQ=255)
                                                            cGTTGCGTTTGTGCAGGTGTTGGACTTCGAGTTTGTTCTTGCTGTGGTTTCTCACTATGCGGTCAGTGCTTGCGCGGCGCCAGGTAGCCGAGCCAGCGGCGTTCGTCCATCTTGAACAGGCGCACCAGGATGTAgg                                                                          <  2:162763/136‑1 (MQ=255)
                                                              tAGCGCTTGTGCAGGTCTTGGACTTCGAGTTTGTACATGCTGTCGATTCTCACAAAGCGGTCAGTGCTTGCGCGGCGCCAGGTAGCCGAGCCAGCGGCGTTCGGCCATCTTGAACAGGCGCACCAGGATGAAGGt                                                                         >  3:136232/1‑135 (MQ=255)
                                                              tAGCGCTTGTGCAGGTCTTGGACTTCGAGTTTGTACATGCTGTCGATTCTCACAAAGCGGTCAGTGCTTGCGCGGCGCCAGGTAGCCGAGCCAGCGGCGTTCGGCCATCTTGAACAGGCGCACCAGGATGAAGGt                                                                         >  4:291641/1‑135 (MQ=255)
                                                              tAGCGCTTGTGCAGGTCTTGGACTTCGAGTTTGTACATGCTGTCGATTCTCACAAAGCGGTCAGTGCTTGCGCGGCGCCAGGTAGCCGAGCCAGCGGCGTTCGGCCATCTTGAACAGGCGCACCAGGATGAAGGt                                                                         >  1:275926/1‑135 (MQ=255)
                                                              tAGCGCTTGTGCAGGTCTTGGACTTCGAGTTTGTACATGCTGTCGATTCTCACAAAGCGGTCAGTGCTTGCGCGGCGCCAGGTAGCCGAGCCAGCGGCGTTCGGCCATCTTGAACAGGCGCACCAGGATGAAGGt                                                                         >  3:70021/1‑135 (MQ=255)
                                                                gcgcTTGTGCAGGTCTTGGACTTCGAGTTTGTACATTCTGTCGATTCTCACAAAGCGGTCAGTGCTTGCGCGGCGCCAGGTAGCCGAGCCAGCGGCGTTCGGCCATCTTGAACAGGCGCACCAGGATGAAGGTCa                                                                       <  3:268445/135‑1 (MQ=255)
                                                                gcgcTTGTGCAGGTCTTGGACTTCGAGTTTGTACATGCTGTCGATTCTCACAAAGCGGTCAGTGCTTGCGCGGCGCCAGGTAGCCGAGCCAGCGGCGTTCGGCCATCTTGAACAGGCGCACCAGGATGAAGGTCAg                                                                      <  8:222273/136‑1 (MQ=255)
                                                                gcgcTTGTGCAGGTCTTGGACTTCGAGGTTGTACATGCTGTCGATTCTCACAAAGCGGTCAGGGATTGCGCGGCTCCAGGTATCCGAGCCAGCGGCGTTCGGCCATCTTGAACAGGCGCACCAGGATGAAGGTCAg                                                                      <  3:81108/136‑1 (MQ=255)
                                                                gcgcGTGTGCAGGTCTTGGACTTCGAGTTTGTACTTGCTGTCGATTCTCACAAAGCGGTCAGTGCTTGCGCGGCGCCAGGTAGCCGAGCCAGCGGCGTTCGGCCATCTTGAACAGGCGCACCAGGATGAAGGTCAg                                                                      <  6:229883/136‑1 (MQ=255)
                                                                      gtgCAGGTCTTGGACTTCGAGTTTGTACATGCTGTCGATTCTCACAAAGCGGTCAGTGCTTGCGCGGCGCCAGGTAGCCGAGCCAGCGGCGTTCGGCCATCTTGAACAGGCGCACCAGGATGAAGGTCAGGcac                                                                  <  6:146059/134‑1 (MQ=255)
                                                                         cAGGTCTTGGACTTCGAGTTTGTACATGCTGTCGATTCTCACAAAGCGGTCAGTGCTTGCGCGGCGCCAGGTAGCCGAGCCAGCGGCGTTCGGCCATCTTGAACAGGCGCACCAGGATGAAGGTCAGGCACAGGTa                                                             <  7:85791/136‑1 (MQ=255)
                                                                                 ggACTTCGAGTTTGTACATGCTGTCGATTCTCACAAAGCGGTCAGTGCTTGCGCGGCGCCAGGTAGCCGAGCCAGCGGCGTTCGGCCATCTTGAACAGGCGCACCAGGATGAAGGTCAGGCACAGGTAGAacac                                                       <  2:124084/134‑1 (MQ=255)
                                                                                  gACTTCGAGTTTGTACATGCTGTCGATTCTCACAACGCGGTCAGTGCTTGCGCGGCGCCAGGTAGCCGAGCCAGCGGCGTTCGGCCATCTTGAACAGGCGCACCAGGATGAAGGTCAGGCACAGGTAGAACAcgcc                                                    <  6:236905/136‑1 (MQ=255)
                                                                                  gACTTCGAGTTTGTACATGCTGTCGATTCTCACAAAGCGGTCAGTGCTTGCGTGGCGCCAGGTAGCCGAGCCAGCGGCGTTCGGCCATCTTGAACAGGCGCACCAGGATGAAGGTCAGGCACAGGTAGAACAcgc                                                     <  2:141919/135‑1 (MQ=255)
                                                                                  gACTTCGAGTTTGTACATGCTGTCGATTCTCACAAAGCGGTCAGTGCTTGCGTGGCGCCAGGTAGCCGAGCCAGCGGCGTTCGGCCATCTTGAACAGGCGCACCAGGATGAAGGTCAGGCACAGGTAGAACAcgc                                                     <  2:137883/135‑1 (MQ=255)
                                                                                  gACTTCGAGTTTGTACATGCTGTCGATTCTCACAAAGCGGTCAGTGCTTGCGCGGCGCCAGGTAGCCGAGCCAGCGGCGTTCGGCCATCTTGAACAGGCGCACCAGGATGAAGGTCAGGCACAGGTAGAACAcgc                                                     <  4:139769/135‑1 (MQ=255)
                                                                                  gACTTCGAGTTTGTACATGCTGTCGATTCTCACAAAGCGGTCAGTGCTTGCGCGGCGCCAGGTAGCCGAGCCAGCGGCGTTCGGCCATCTTGAACAGGCGCACCAGGATGAAGGTCAGGCACAGGTAGAACAcgc                                                     <  3:291641/135‑1 (MQ=255)
                                                                                  gACTTCGAGTTTGTACATGCTGTCGATTCTCACAAAGCGGTCAGTGCTTGCGCGGCGCCAGGTAGCCGAGCCAGCGGCGTTCGGCCATCTTGAACAGGCGCACCAGGATGAAGGTCAGGCACAGGTAGAACAcgc                                                     <  3:118722/135‑1 (MQ=255)
                                                                                    cTTCGAGTTTGTACATGCTGTCGATTCTCACAAAGCGGTCAGTGCTTGCGCGGCGCCAGGTAGCCGAGCCAGCGGCGTTCGGCCATCTTGAACAGGCGCACCAGGATGAAGGTCAGGCACAGGTAGAACAcgcccg                                                  >  4:252026/1‑136 (MQ=255)
                                                                                    cTTCGAGTTTGTACATGCTGTCGATTCTCACAAAGCGGGCAGTGCTTGCGCGGCGCCAGGTAGCCGAGCCAGCGGCGTTCGGCCATCTTGAACAGGCGCACCAGGATGAAGGTCAGGCACAGGTAGAACAcgcccg                                                  <  3:72283/136‑1 (MQ=255)
                                                                                          gTTTGTACATGCTGTCGATTCTCACAAAGCGGTCAGTGCTTGCGCGGCGCCAGGTAGCCGAGCCAGCGGCGTTCGGCCATCTTGAACAGGCGCACCAGGATGAAGGTCAGGCACAGGTAGAACACGCCCGCCGTGa                                            >  5:108100/1‑136 (MQ=255)
                                                                                          gTTTGTACATGCTGTCGATTCTCACAAAGCGGTCAGTGCTTGCGCGGCGCCAGGTAGCCGAGCCAGCGGCGTTCGGCCATCTTGAACAGGCGCACCAGGATGAAGGTCAGGCACAGGTAGAACACGCCCGCCGTGa                                            <  4:117098/136‑1 (MQ=255)
                                                                                          gTTTGTACATGCTGTCGATTCTCACAAAGCGGTCAGTGCTTGCGCGGCGCCAGGTAGCCGAGCCAGCGGCGTTCGGCCATCTTGAACAGGCGCACCAGGATGAAGGTCAGGCACAGGTAGAACACGCCCGCCGTGa                                            >  5:276762/1‑136 (MQ=255)
                                                                                          gTTTGTACATGCTGTCGATTCTCACAAAGCGGTCAGTGCTTGCGCGGCGCCAGGTAGCCGAGCCAGCGGCGTTCGGCCATCTTGAACAGGCGCACCAGGATGAAGGTCAGGCACAGGTAGAACACGCCCGCCGTGa                                            >  8:267504/1‑136 (MQ=255)
                                                                                                aCATGCTGTCGATTCTCACAAAGCGGTCAGTGCTTGCGCGGCGCCAGGTAGCCGAGCCAGCGGCGTTCGGCCATCTTGAACAGGCGCACCAGGATGAAGGTCAGGCACAGGTAGAACACGCCCGCCGTGATATAgg                                      >  4:44043/1‑136 (MQ=255)
                                                                                                   tGCTGTCGATTCTCACAAAGCGGTCAGTGCTTGCGCGGCGCCAGGTAGCCGAGCCAGCGGCGTTCGGCCATCTTGAACAGGCGCACCAGGATGAAGGTCAGGCACAGGTAGAACACGCCCGCCGTGATATAGGCCt                                   <  8:37614/136‑1 (MQ=255)
                                                                                                   tGCTGTAGATTCTCACAAAGCTGTTAGTGCTTGCGCGGCGCCAGGTAGCCTAGCCAGCGGCGTTCGGCCATCTTGAACAGGCGCACCAGGATGAAGGTCAGTCACAGGTAGAACACGCCCGCCGTGATATAGGCCt                                   <  2:260617/136‑1 (MQ=255)
                                                                                                      tGTCGATTCTCACAAAGGGGTCAGTGCTTGCGCGGCGCAAGGTAGCCGAGCCAGCGGCGTTCGGCCTTCTTGAACAGGCGCACCAGGATGAAGGTCAGGCACAGGTAGAACACGCCCGCCGTGTTATAGGCCTCGa                                <  2:270304/136‑1 (MQ=255)
                                                                                                       gTCGATTCTCACAAAGCGGTCAGTGCTTGCGCGGCGCCAGGTAGCCGAGCCAGCGGCGTTCGGCCATCTTGAACAGGCGCACCAGGATGAAGGTCAGGCACAGGTAGAACACGCCCGCCGTGATATAGGCCTCGa                                >  4:196343/1‑135 (MQ=255)
                                                                                                        tCGATTCTCACAAAGCGTTCAGTGCTTGCGCGGCGCCAGGTAGCCGAGCCAGCGGCGTTCGGCCATCTTGAACAGGCGCACCAGGATGAAGGTCAGGCACAGGTAGAACACGCCCGCCGTGATATAGGCCTCGaaa                              <  5:266340/136‑1 (MQ=255)
                                                                                                        tCGATTCTCACAAAGCGGTCAGTGCTTGCGCGGCGCCAGGTAGCCGAGCCAGCGGCGTTCGGCCATCTTGAACAGGCGCACCAGGATGAAGGTCAGGCACAGGTAGAACACGCCCGCCGTGATATAGGCCTCGa                                <  5:48656/134‑1 (MQ=255)
                                                                                                        tCGATTCTCACAAAGCGGTCAGTGCTTGCGCGGCGCCAGGTAGCCGAGCCAGCGGCGTTCGGCCATCTTGAACAGGCGCACCAGGATGAAGGTCAGGCACAGGTAGAACACGCCCGCCGTGATATAGGCCTCGa                                <  8:68923/134‑1 (MQ=255)
                                                                                                        tCGATTCTCACAAAGCGGTCAGTGCTTGCGCGGCGCCAGGTAGCCGAGCCAGCGGCGTTCGGCCATCTTGAACAGGCGCACCAGGATGAAGGTCAGGCACAGGTAGAACACGCCCGCCGTGATATAGGCCTCGa                                <  8:4234/134‑1 (MQ=255)
                                                                                                        tCGATTCTCACAAAGCGGTCAGTGCTTGCGCGGCGCCAGGTAGCCGAGCCAGCGGCGTTCGGCCATCTTGAACAGGCGCACCAGGATGAAGGTCAGGCACAGGTAGAACACGCCCGCCGTGATATAGGCCTCGa                                <  8:162983/134‑1 (MQ=255)
                                                                                                        tCGATTCTCACAAAGCGGTCAGTGCTTGCGCGGCGCCAGGTAGCCGAGCCAGCGGCGTTCGGCCATCTTGAACAGGCGAACCAGGATGAAGGTCAGGCACAGGTAGAACACGCCCGCCGTGATATAGGCCTCGa                                <  6:120937/134‑1 (MQ=255)
                                                                                                           aTTCTCACAAAGCGGTCAGTGCTTGCGCGGCGCCAGGTAGCCGAGCCAGCGGCGTTCGGCCATCTTGAACAGGCGCACCAGGATGAAGGTCAGGCACAGGTAGAACACGCCCGCCGTGATATAGGCCTCGAAAGGc                           <  7:267504/136‑1 (MQ=255)
                                                                                                            ttCTCACAAAGCGGTCAGTGCTTGCGCGGCGCCAGGTAGCCGAGCCAGCGGCGTTCGGCCATCTTGAACAGGCGCACCAGGATGAAGGTCAGGCAGAGGTAGAACACGCCCGCCGTGATATAGGCCTCGAAAgg                            >  1:200158/1‑134 (MQ=255)
                                                                                                            ttCTCACAAAGCGGTCAGTGCTTGCGCGGCGCCAGGTAGCCGAGCCAGCGGCGTTCGGCCATCTTGAACAGGCGCACCAGGATGAAGGTCAGGCACAGGTAGAACACGCCCGCCGTGATATAGGCCTCGAAAgg                            >  7:152499/1‑134 (MQ=255)
                                                                                                            ttCTCACAAAGCGGTCAGTGCTTGCGCGGCGCCAGGTAGCCGAGCCAGCGGCGTTCGGCCATCTTGAACAGGCGCACCAGGATGAAGGTCAGGCACAGGTAGAACACGCCCGCCGTGATATAGGCCTCGAAAGGc                           <  7:52345/135‑1 (MQ=255)
                                                                                                                 acaAAGCGGTCAGTGCTTGCGCGGCGCCAGGTAGCCGAGCCAGCGGCGTTCGGCCATCTTGAACAGGCGCACCAGGATGAAGGTCAGGCACAGGTAGAACACGCCCGCCGTGATATAGGCCTCGAAAGGCAGgtag                     <  2:60642/136‑1 (MQ=255)
                                                                                                                 acaAAGCGGTCAGTGCTTGCGCGGCGCCAGGTAGCCGAGCCAGCGGCGTTCGGCCATCTTGAACAGGCGCACCAGGATGAAGGTCAGGCACAGGTAGAACACGCCCGCCGTGATATAGGCCTCGAAAGGCAGgtag                     <  7:25407/136‑1 (MQ=255)
                                                                                                                 acaAAGCGGTCAGTGCTTGCGCGGCGCCAGGTAGCCGAGCCAGCGGCGTTCGGCCATCTTGAACAGGCGCACCAGGATGAAGGTCAGGCACAGGTAGAACACGCCCGCCGTGATATAGGCCTCGAAAGGCAGgtag                     <  5:145029/136‑1 (MQ=255)
                                                                                                                 acaAAGCGGTCAGTGCTTGCGCGGCGCCAGGTAGCCGAGCCAGCGGCGTTCGGCCATCTTGAACAGGCGCACCAGGATGAAGGTCAGGCACAGGTAGAACACGCCCGCCGTGATATAGGCCTCGAAAGGCAGgtag                     <  6:146545/136‑1 (MQ=255)
                                                                                                                            aGTGCTTGCGCGGCGCCAGGTAGCCGAGCCAGCGGCGTTCGGCCATCTTGAACAGGCGCACCAGGATGAAGGTCAGGCACAGGTAGAACACGCCCGCCGTGATATAGGCCTCGAAAGGCAGGTAGTACTGGGCAtt          >  3:251729/1‑136 (MQ=255)
                                                                                                                                   gagcggctCCAGGAAGCCGAGCAAGCGGCGTTCGGCCAGCTTGAACAGGCGCACCAGGATGAAGGTCAGGCACAGGTAGAACACGCCCGCCGTGATATAGGCCTCGAAAGGCAGGTAGTACTGGGCATTGACCGt    <  2:150193/133‑1 (MQ=255)
                                                                                                                                    cgcgGCGCCAGGTAGCCGAGCCAGCGGCGTTCGGCCATCTTCAACAGGCGCACCAGGATGAAGGTCAGGCACAGGTAGAACACGCCCGCCGTGATATAGGCCTCGAAAGGCAGGTAGTACTGGGCATTGACCGTgc  <  5:30341/136‑1 (MQ=255)
                                                                                                                                      |                                                                                                                                     
TGCTGATGACGTCGCCGGCCTTGGCCGCCAGCGACACGCCCTTGAGCACTTCGTGGCTGCCGTAGCGCTTGTGCAGGTCTTGGACTTCGAGTTTGTACATGCTGTCGATTCTCACAAAGCGGTCAGTGCTTGCGCGGCGCCAGGTAGCCGAGCCAGCGGCGTTCGGCCATCTTGAACAGGCGCACCAGGATGAAGGTCAGGCACAGGTAGAACACGCCCGCCGTGATATAGGCCTCGAAAGGCAGGTAGTACTGGGCATTGACCGTGC  >  ACB143/5087704‑5087971

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑