Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB143 5,693,153 T→G 33.6% R228R (CGT→CGG)  pyrC' → Dihydroorotase‑like protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1435,693,1530TG33.6% 26.7 / 2.7 39R228R (CGT→CGG) pyrC'Dihydroorotase‑like protein
Reads supporting (aligned to +/- strand):  ref base T (9/16);  new base G (4/10);  total (13/26)
Fisher's exact test for biased strand distribution p-value = 7.33e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCGCGACCTGGCCCAGGGCGGCCTGGCCCACGAAGGTGCCATGGCCAGCTTCCTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGC‑TGCTGGTGGAACAGAGCGGCGTGCGTGCGCACTTCAGCCAGATCACCAGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGTTCTCC  >  ACB143/5693018‑5693288
                                                                                                                                        |                                                                                                                                       
ccGCGACCTGGCCCAGGGCGGCCTGGCCCACGAAGGTGCCATGGCCAGCTTCCTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGC‑TGCTGGTGGAACAGAGCGgcgtgcgt                                                                                                                                         <  6:175872/136‑1 (MQ=255)
     aCCTGGCCCAGGGCGGCCTGGCCCACGAAGGTGCCATGGCCAGCTTCCTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGC‑TGCTGGTGGAACAGAGCGGCGtgggggcggc                                                                                                                                    >  5:203227/1‑134 (MQ=255)
      ccTGGCCCAGGGCGGCCTGGCCCACGAAGGTGCCATGGCCAGCTTCCTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGC‑TGCTGGTGGAACAGAGCGGCGTGCGTgcgccc                                                                                                                                   >  1:16931/1‑134 (MQ=255)
      ccTGGCCCAGGGCGGCCTGGCCCACGAAGGTGCCATGGCCAGCTTCCTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCCTGGCCCTGGCGCGCAACCTGC‑TGCTGGTGGAACAGAGCGGCGtgcgtgcg                                                                                                                                      >  5:253854/1‑133 (MQ=255)
          gCCCAGGGCGGCCTGGCCCACGAAGGTGCCATGGCCAGCTTCCTCGGCCTGCCGGGCATCCCGGAGAGCGCCGATACCGTGGCCCTGGCGCGCAACCTGC‑TGCTGGTGGAACAGAGCGGCGTGCGTGCGCACTTc                                                                                                                                <  1:3480/135‑1 (MQ=255)
          gCCCAGGGCGGCCTGGCCCACGAAGGTGCCATGGCCAGCTTCCTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGC‑TGCTGGTGGAACAGAGCGGCGTGCGTGCGCACTTc                                                                                                                                <  2:65331/135‑1 (MQ=255)
            ccAGGGCGGCCTGGCCCACGAAGGTGCCATGGCCAGCTTCCTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGC‑TGCTGGTGGAACAGAGCGGCGTGCGGGCGCCCTTGAGc                                                                                                                             >  4:101217/1‑136 (MQ=255)
            ccAGGGCGGCCTGGCCCACGAAGGTGCCATGGCCAGCTTCCTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGC‑TGCTGGTGGAACAGAGCGGCGTGCGGGCGCCCGTCAGc                                                                                                                             >  4:201062/1‑136 (MQ=255)
                  cggcCTGGCCCACGAAGGTGCCATGGCCAGCTTCCTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGC‑TGCTGGTGGAACAGAGCGGCGTGCGTGCGCACTTCCGCCAGAt                                                                                                                        >  4:232226/1‑135 (MQ=255)
                   ggcCTGGCCCACGAAGGTGCCATGGCCAGCTTCCTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGC‑TGCTGGTGGAACAGAGCGGCGTGCGTGCGCCCGTCCGCCCGATCa                                                                                                                      >  7:202394/1‑136 (MQ=255)
                       tGGCCCACGAAGGTGCCATGGCCAGCTTCCTCGGCCTGCCGGGCATCCCGGAGCGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGC‑TGCTGGTGGAACAGAGCGGCGTGCGTGCGCACGTCCGCCAGCTAACCAg                                                                                                                  >  5:57455/1‑136 (MQ=255)
                       tGGCCCACGAAGGTGCCATGGCCAGCTTCCTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGC‑TGCTGGTGGAACAGAGCGGCGTGCGTGCGCACTTCAGCCAGATCAcccg                                                                                                                  >  6:214168/1‑134 (MQ=255)
                          cccACGATGGTGCCATGGCCAGCTTCCTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGC‑TGCTGGTGGAACAGAGCGGCGTGCGTGCGCACTTCAGCCAGATCACCAgcgc                                                                                                               <  3:101028/136‑1 (MQ=255)
                                       cATGGCCAGCTTCCTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGC‑TGCTGGTGGAACAGAGCGGCGTGCGGGCGCACTTCCGCCCGATCACCCGCCCCCGTGGCGCGCgg                                                                                                  >  2:5143/1‑136 (MQ=255)
                                             cAGCTTCCTGGGCCTGCCGGGCATCGCGGAGAGCGCCGAACCCGGGGCCCTGGCGCGCAACCTGC‑TGCTGGTGGAACAGAGCGGGGGGCGTGCGCACTTCAGCCAGACCACCAGCGCCCGTGGCGCGCGGCTGATc                                                                                            <  1:298654/136‑1 (MQ=255)
                                                 ttCCTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGC‑TGCTGGTGGAACAGAGCGGCGTGCGTGCGCCCTTCAGCCCGATCACCCGCGCCCGGGGCGCGCGGCGGCTCgccc                                                                                        >  3:37995/1‑136 (MQ=255)
                                                   ccTCGGCGTGCCGGCCCTCCGGGAGCGCCCCGAACCCGGGGCCCGGGCGCGCAACCTGG‑TGCTGGTGGAACTCTGCGGGGTGCTGGCGCACTTCAGCCAGACCCCCCGCGCCCGTGGCGCGCGGCTGATCgcccag                                                                                      <  4:229993/136‑1 (MQ=255)
                                                   ccTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGC‑TGCTGGTGGAACAGAGCGGCGTGCGGGCGCCCATAAGCCCACTAACCACCGCCCCGGGCGCCCGGCAGCTAgcccac                                                                                      >  2:155245/1‑135 (MQ=255)
                                                     gcgccgtGCGGGGCATCCCGGAGAGCGCCTAAACCGTGGCCCGGGCGCGAAACCTGC‑TGCTGGGGCAACTGAGCGGCGTGCGTGCGCACTTCAGCCACACCCCCAGCGCCCGTGGCGCGCGGCTGATCgcccaggc                                                                                    <  2:56411/130‑1 (MQ=255)
                                                     gcGGCCTGCCGGGCATCCCGGAGAGCGCCGAACCGGGGGCCCGGGCGCGCAACCTGC‑TGCTGGGGGAACACCGCGGCGGGGGGGCGCACTACAGCCAGAACCCCCGCGCCCGGGGCGCGCGGCTGATCgcccaggc                                                                                    <  8:31371/135‑1 (MQ=255)
                                                       ggccgcccGGGCATCCCGGAGAGCGCCTAAACCGGGGCCCGGGCGCGCACCCGGC‑TGCGGGTGGAACCGCGCGGCGGGGGTGCGCAATCCCGCCAGAACACCCGCGCCCGGGGCGCGCGGCTGATCgcccaggc                                                                                    <  4:82272/128‑1 (MQ=255)
                                                       ggCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGGGGCCCTGGCGCGCAACGTGC‑GGCTGGTGGAACAGAGCGGGGTGCGTGCGCACTTCAGCCAGATCACCAGCGCCCGTGGCGCGCGGCTGATCgcccaggc                                                                                    <  5:195098/134‑1 (MQ=255)
                                                         ccggCCGGCCATCCCGGACCGCCCCGAACCGGGGGCCCTGGCGCGCAACCTGC‑TGCTGGGGTAACAGCGCGGGGGGCGGGCCCACTGCCGCCCGAGCCCCCGCGCCCGGGGCGCGCGGCTGATCgcccaggcccag                                                                                <  6:199519/133‑1 (MQ=255)
                                                               gggctgcccGGAGAGCGCCTAAACGGGGGCGGGGGCGCGCACCGTGG‑TGCTGGGGGAACCGCGGGGCGGGCGGGCGCAATCCCCCCCGACCACCCGGGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTg                                                                          <  2:107956/130‑1 (MQ=255)
                                                                       cGGTGAGCGCCTAAACCGGGGCCCTGGCGCGCACCCTGC‑GGCGGGTGGAACAGAGGGGCGTGCGGGCGCACTTCAGCCCGAGCCCCCGCGCCCGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGcc                                                                  <  5:69698/136‑1 (MQ=255)
                                                                        ggAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGC‑TGCTGGTGGAACAGAGCGGCGTGCGTGCGCACTTCAGCCAGATCACCAGCGCCCGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCg                                                                 <  7:255648/136‑1 (MQ=255)
                                                                             ggccgtaaaCGGGGGCCCTGGCGCG‑AAACTGCGTGGTGGTGCACCCGCGGGGCGGGCGGGGGCACTACAGCCAGACCACCCGCGCCGTGGGGGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAAc                                                            <  2:266736/130‑1 (MQ=255)
                                                                             gcgcCGAAACCGTGGCCCTGGCGCGCAACCTGC‑TGCTGGTGGAACAGAGCGGCGTGCGTGCGCACTTCAGCCAGATCACCAGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAAc                                                            <  5:262354/136‑1 (MQ=255)
                                                                             gcgcCGAAACCGTGGCCCTGGCGCGCAACCTGC‑TGCGGGTGGAACAGAGCGGCGGGCGGGCGCACTTCAGCCAGATCACCCGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAAc                                                            <  7:52109/136‑1 (MQ=255)
                                                                             gcgcCGAAACCGGGGCCCTGGCGCGCAACCTGC‑TGCTGGGGGACCAGAGCGGCGTGCGTGCGCACTTCAGCCAGAGCACCAGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAAc                                                            <  4:223893/136‑1 (MQ=255)
                                                                                       gggggCCGTGGCGCGCAACCTGC‑GGGTGGGGCAACCGCGGGGGGGGGGGGCGCACTGCAGCCAGATCCCCCGCGCCGGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTa                                                  <  3:201062/133‑1 (MQ=255)
                                                                                       ggTGGCCCTGGCGCGAAACGTGC‑TGCTGGTGTAACAGAGCGGCGTGCGTGCGAACTACCGCCATATCACCCGCGCCGGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTa                                                  <  5:251366/135‑1 (MQ=255)
                                                                                       cggggCCGTGGCGCGCAACCTGG‑TGCTGGTGGAACCGCGCGGCGGGCGGGCGCAATTCACCCAGACCCCCCGCGCGCGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTa                                                  <  4:18293/133‑1 (MQ=255)
                                                                                       cggggCCCTGGCGCGCAACCTGC‑TGCTGGTGGAACAGAGCGGCGTGCGTGCGCACTTCAGCCAGATCACCAGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTa                                                  <  6:274609/133‑1 (MQ=255)
                                                                                       cGTGGCCCTGGCGCGCACCCTGC‑TGCGGGGTGAACAGTGCGGCGGGGGTGCGAACTTCAGCCAGATCACCCGCGCCGGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTa                                                  <  2:90441/136‑1 (MQ=255)
                                                                                            cccTGGCGCGCAACCTGC‑TGCTGGGGTAACAGAGCGGCGGGCGTGCGCACTTCAGCCAGATCACCAGCGCCCGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCt                                             <  6:62708/136‑1 (MQ=255)
                                                                                                   gcgcAACCTGC‑TGCTGGTGGAACAGAGCGGCGTGCGTGCGCCCTTCAGCCAGATCACCCGCGCCCGTGGCGCGCGGCTGCTCGCCCCGGCCCCGGCCGTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTAcccg                                      >  1:176015/1‑134 (MQ=255)
                                                                                                    cgcAACCTGC‑TGCTGGTGGAACAGAGCGGCGTGCGTGCGCCCATAAGCCCGATCACCAGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCCGGCCGTGGGCCTGCCGGTAACCGCCGCTGTAGCGCGGTTCCAGc                                     >  7:21306/1‑136 (MQ=255)
                                                                                                     gcAACCTGC‑GGCTGGGGGAACAGAGGGGCGTGGGTGCGCACTTCAGCCAGATCACCCGCGCCGGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCt                                    <  7:78405/136‑1 (MQ=255)
                                                                                                                            gagCGGCGTGCGTGCGCACTTCAGCCACACCACCCGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTg              <  1:37752/136‑1 (MQ=255)
                                                                                                                            gagCGGCGTGCGGGCGAACGGCACCCACATCACCCCCGCCCGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCGGTACCAGCTGATCCTCACGGACGAGTCGCTg              <  6:84586/136‑1 (MQ=255)
                                                                                                                              gcggcgTGCGTGCGCACTTCAGCCAGATCACCAGCGCCCGTGGCGCGCGGCTGCTCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACCGACGAGTCGCTgcg            >  7:78720/1‑136 (MQ=255)
                                                                                                                                   gtgcgtgcGCACTTCAGCCAGATCACCAGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGt       <  8:215020/136‑1 (MQ=255)
                                                                                                                                   gggggtgcgAACTTCAGCCATAGCACCAGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGt       <  2:22748/132‑1 (MQ=255)
                                                                                                                                   gggcgtgcgAAGTTCGCCCAGATCCCCCGCGCCGGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGt       <  4:294955/134‑1 (MQ=255)
                                                                                                                                   gggcgggcgCACTCCAGCCAGATCACCCGCGCCCGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGt       <  2:70034/134‑1 (MQ=255)
                                                                                                                                   gggcgggccCACTTCCCCCCGACCACCCGCGCCCGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGt       <  1:139922/134‑1 (MQ=255)
                                                                                                                                     gcgtgcgCACTTCAGCCAGATAACCAGCGCCCGTGGCGCGCGGCTGATAGCCCAGGCCCCGGCCTTGGGCCTGCCGGTAACCGCCGAGGTATCGCTGTACCAGCTGATCCTCACGGACTAGTCGCTGCGCGAGtta     >  2:81688/1‑135 (MQ=255)
                                                                                                                                     gcgtgcgCACTTCAGCCAGACCACCCGCGCCCGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGTtc     <  6:203227/136‑1 (MQ=255)
                                                                                                                                     gcgtgcgCACATCCGCCAGATCACCAGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCGTGGGCCTTCCGGGAACCGCCGCTGTAGCGCTGTACCCGCTGATCCTCACGGACGCGTCGCTGCGCGAGTtc     >  7:150129/1‑136 (MQ=255)
                                                                                                                                        tgcgCACTTCAGCCAGATCACCAGCGCCCGTGGGGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGTTCTcc  <  8:25409/136‑1 (MQ=255)
                                                                                                                                        |                                                                                                                                       
CCGCGACCTGGCCCAGGGCGGCCTGGCCCACGAAGGTGCCATGGCCAGCTTCCTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGC‑TGCTGGTGGAACAGAGCGGCGTGCGTGCGCACTTCAGCCAGATCACCAGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGTTCTCC  >  ACB143/5693018‑5693288

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑