| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | ACB143 | 5,222,515 | 0 | T | A | 41.9% | 24.9 / 11.9 | 37 | intergenic (‑138/+38) | cntO_7/CJ019_04917 | Metal‑pseudopaline receptor CntO/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base T (1/17); new base A (12/1); total (17/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.14e-06 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
ACGCAGACCTGTGAGGGGGTGATTATTGCGAATCAATCGCACTTTCACTCATTACACGGATGCCACGTGCTGTTACCTCACCTGTGCCTGGAAATATTTTTGC‑TGTGGCGGCCTCTTCG‑C‑GGG‑T‑A‑AA‑CCCGCCCCTA‑CGGGGGGT‑TTACCC‑GTGAA‑GAGGCTGGCACAGGCAACTACCGAACCAGGGTCAAACGCCCCAACACGGTCTGCCGTGAAAAACCCATCACCTTGCCCAGCGAATCCAGCGCCAGCAGCGGCTCGGCCACCGGG > ACB143/5222380‑5222649 | acgcttaCCTTTGAGGTTGTGAGTATTGCGAATCACTCGCACTTTCACTCATTAGACGGATGCCACGTGCTGTTCCCTCACCTGTGCCTCGAAATATTTTTGC‑TGTGGCGGCCTCTGCG‑C‑TTG‑T‑A‑AA‑CCCGCCCCt < 7:186578/130‑1 (MQ=255)aCGCAGACCTGTGAGGGTGTGATTATTGCGAATCAATCGCACTTTCACTCATTACACGGATGCCACGTGCTGTTACCTCACCTGTGCCTGGAAATATTTTTGC‑TGTGGCGGCCTCTTCG‑C‑GGG‑T‑A‑AA‑CCCGCCCCt < 6:31226/136‑1 (MQ=255)aCGCAGACCTGTGAGGGGGTGAGTATTGCGAATCAATCGCACTTTCACTCATTACACGGATGCCACGTGCTGTTACCTCACCTGTGCCTGGAAATATTTTTGC‑TGTGGCGGCCTCTTCG‑C‑GGG‑T‑A‑AA‑CCCGCCCCt < 6:40741/136‑1 (MQ=255) gCAGACCTGTTATGGGGTGATTATTGCGAATCAATCGAACTTTCCATCATTCCACTGATGCAAAGTGCTGTTACCTCACATGTGCCTGGAATTATTTTTGA‑TGTGGGGGCCTCTTAG‑C‑GTG‑T‑A‑AA‑CCCTCCCCTA‑c < 8:231935/136‑1 (MQ=255) gCAGACCTGTGAGGGGGTGATTATTGCGAATCAATCGCACTTTCACTCATTACACGGATGCCACGTGCTGTTACCTCACCTGTGCCTGGAAATATTTTTGC‑TGTGGCGGCCTCTTCG‑C‑GGG‑T‑A‑AA‑CCCGCCCCTA‑c < 6:125834/136‑1 (MQ=255) cAGACCTGGGAGGGGGTGATTATTGCGAATCAATCGCACTTTCACTCATTACACGGATGCCACGTGCTGTTACCTCACCTGTGCCTGGAAATATTTTTGC‑TGTGGCGGCCTCTTCG‑C‑GGG‑T‑A‑AA‑CCCGCCCCTA‑c < 3:361263/135‑1 (MQ=255) tgtgAGGGGGTGATTATTGCGAATCAATCGCACTTTCACTCATTACACGGATGCCACGTGCTGTTACCTCACCTGTGCCTGGAAATATTTTTGC‑TGTGGCGGCCTCTTAG‑C‑GGG‑T‑T‑AA‑AACCCCCCAA‑Aggggggg > 6:318687/1‑135 (MQ=255) gggggTGATTATTGCGAATCAATCGCACTTTCACTCATTACACGGATGCCACGTGCTGTTACCTCACCTGTGCCTGGAAATATTTTTGC‑TGTGGCGGCCTCTTCG‑C‑GGGTT‑A‑AA‑CCCCCCCCAC‑ACGGGGGT‑TTa > 1:340540/1‑135 (MQ=255) gggggTGATTATTGCGAATCAATCGCACTTTCACTCATTACACGGATGCCACGTGCTGTTACCTCACCTGTGCCTGGAAATATTTTTGC‑TGTGGCGGCCTATTCGCC‑GGG‑A‑A‑AA‑ACCCCCCCAC‑AGGGGGGT‑TTa > 7:349446/1‑135 (MQ=255) ggTGATTATTGCGAATCAATCGCACTTTCACTCATTACACGGATGCCACGTGCTGTTACCTCACCTGTGCCTGGAAATATTTTTGC‑TGTGGCGGCCTCTTCG‑C‑GGG‑T‑A‑AA‑CCCGCCCCTA‑CGGGGGGT‑TTACCC‑Gt < 2:302844/136‑1 (MQ=255) ggTGATTATTGCGAATCAAGCGCACTTTCACACATTACACGGATGCCACGGGCTGTTACCTCACCTGTGCCTGGAAATATTTTTGC‑TGTGGCGGCCTCTTCG‑C‑GGG‑T‑A‑AA‑CCCGCCCCTA‑CGGGGGGT‑TAACCC‑Gt < 8:115991/136‑1 (MQ=255) tGATTATTGCGAATCAATCGCACTTTCACTCATTACACGGATGCCACGTGCTGTTACCTCACCTGTGCCTGGAAATATTTTTGC‑TGTGGCGGCCTCTTCG‑C‑GGGTT‑A‑AA‑CCCCCCCCAC‑GGGGGGGT‑TTACCC‑gg > 1:35192/1‑134 (MQ=255) attattGCGAATCAATCGCACTTTCACTCATTACACGGATGCCACGTGCTGTTACCTCACCTGTGCCTGTAAATATTTTTGC‑TGTGGCGGCCTCTTCG‑C‑GGGTT‑A‑AA‑CCCCCCCGAA‑CGGGGGGT‑TTACCC‑gg > 7:67821/1‑132 (MQ=255) attattGCGAATCAATCGCACTTTCACTCATTACACGGATGCCACGTGCTGTTACCTCACCTGTGCCTGGAAATATTTTTGC‑TGTGGCGGCCTCTTCG‑CGGGG‑T‑T‑AA‑ACCCCCCCCA‑CGGGGGGG‑TTGccctgga > 4:375176/1‑131 (MQ=255) attattGCGAATCAATCGCACTTTCACTCATTACACGGATGCCACGTGCTGTTACCTCACCTGTGCCTGGAAATATTTTTGC‑TGTGGCGGCCTCTTCG‑CGGGG‑T‑A‑AA‑CCCCCCCCAC‑CGGGGGGT‑TTcccggg > 8:230633/1‑130 (MQ=255) attattGCGAATCAATCGCACTTTCACTCATTACACGGATGCCACGTGCTGTTACCTCACCTGTGCCTGGAAATATTTTTGC‑TGTGGCGGCCTCTTCG‑C‑GGG‑TAA‑AA‑CCCCCCCCAA‑CGGGGGGT‑TTACCC‑ggta > 7:110805/1‑132 (MQ=255) attattGCGAATCAATCGCACTTTCACTCATTACACGGATGCCACGTGCTGTTACCTCACCTGTGCCTGGAAATATTTTTGC‑TGTGGCGGCCTCTTCG‑C‑GGG‑G‑T‑TA‑CCCGCCCCAG‑CGGGGGGTGTTAccgggtgg > 6:271879/1‑130 (MQ=255) attattGCGAATCAATCGCACTTTCACTCATTACACGGATGCCACGTGCTGTTACCTCACCTGTGCCTGGAAATATTTTTGC‑TGTGGCGGCCTCTTCG‑C‑GGG‑G‑ATAA‑CCCCCCCCCA‑GGGGGGGT‑TGAccgggga > 4:27235/1‑130 (MQ=255) atcaatcGCACTTTCACTCATTACACGGATGCCACGTGCTGTTACCTCACCTGTACCTGGAAATATTTTTGC‑TGTGGCGGCCTCTTCG‑CGGGG‑T‑A‑AA‑CCC‑CCCCAA‑CCGGGGGG‑TTTCCC‑CTGTA‑GAGGCggggc > 7:283919/1‑133 (MQ=255) tcaatcGCACTTTCACTCATTACACGGATGCCACGTGCTGTTACCTCACCTGTGCCTGGAAATATTTTTGC‑TGTGGCGGCCTCTTCG‑C‑GGG‑T‑A‑AC‑CCCGCCCCTA‑CGGGGGGT‑TTACCC‑GTGAA‑GAGGCTGGcaca < 5:363811/136‑1 (MQ=255) aatcGCACTTTCACTCATTACACGGATGCCACGTGCTGTTACCTCACCTGTGCCTGGAAATATTTTTGC‑TGTGGCGGCCTCTTCG‑C‑GGG‑T‑A‑AA‑ACCCCCCCGACCGGGGGGT‑TTACCC‑‑TGAA‑CACGCAGGcacacc > 7:225137/1‑134 (MQ=255) ttACACGGATGCCACGTGGTGTTACGTCACCTGTGCCTGGAATTATTTTTGC‑TGTGGGGGCCTCTTCG‑C‑GGG‑T‑A‑AA‑CCCGCCCCTA‑CGGGGGGT‑TTACCG‑GTGAT‑GAGGCTGGCACAGGCAACTACCGAACCAggg < 5:155479/136‑1 (MQ=39) cacGGATGCCACGTGCTGTTACCTCACCTGTGCCTGGAAATATTTTTGC‑TGTGGCGGCCTCTTCG‑C‑GGG‑T‑A‑AA‑CCCGCCCCTA‑CGGGGGGT‑TTACCC‑GTGAA‑GTGGCTGGCACAGGCAACTACCGAACCAGGGTc < 1:259810/135‑1 (MQ=255) tGCCACGTGCTGTTACCTCACCTGTGCCTGGAAATATTTTTGC‑TGTGGCGGCCTCTTCG‑C‑GGGTT‑A‑AA‑CCCCCCCCAC‑CGGGGGGT‑TTCCCC‑G‑GGA‑GTGGGGGGCCCCGGCAACCACCCCACCACGGGCAAAcccc > 1:296253/1‑136 (MQ=255) ccACGTGCTGTTACCTCACCTGTGCCTGGAAATATTTTTGC‑TGTGGCGGCCTCTTCG‑C‑GGG‑T‑A‑AA‑CCCGCCCCTA‑CGGGGGGT‑TTACCC‑GTGAA‑GAGGCTGGCACAGGCAACTACCGAACCAGGGTCAAACGcccc < 7:196586/136‑1 (MQ=255) tCACCTGTGCCTGGAAATATTTTTGC‑TGTGGCAGCCTCTTCG‑C‑GGG‑T‑A‑AA‑ACCCCCCCAA‑CGGGGGGT‑TTTACC‑GTGAA‑GAGGCTTGGACAGGCAACTACCGCACCCCGGGCAAAAGCCCCCACACCGTCTGCCgg > 2:258950/1‑135 (MQ=255) cACCTGTGCCTGTAAATATTTTTGC‑TGGGGCGGCCTCTTCG‑G‑GGG‑A‑A‑AA‑CCCGCCCCTA‑CGGGGGTT‑TTACCC‑GTTAA‑GAGGCTGGCACAGGCAACTACCGAACCAGGGTCAAACGCCCCAACACGGTCTGCCGTg < 7:165518/136‑1 (MQ=255) cACCTGTGCCTGGAAATATTTTTGC‑CGTGGCGGCCTCTTCG‑C‑GGG‑T‑A‑AA‑CCCGCCCCTA‑CGGGGGGT‑TTACCC‑GTGAA‑GAGGCTGGCACAGGCAACTACCGAACCAGGGTCAAACGCCCCAACACGGTCTGCCGTg < 2:109034/136‑1 (MQ=255) gtgCCTGTAAATATTTTTGC‑TGTGGCGGCCTCTCCG‑C‑GGG‑T‑A‑AA‑CCCGCCCCAC‑CGGGGGGT‑TTACCG‑GTGAA‑GTGGCGGGCACAGGCAACTACCGAACCAGGGTCAAACGCCCCAACACGGTCTGCCGTGaaaa < 3:373066/135‑1 (MQ=255) gCCTGTAAATTTTTTTGT‑GGGGGGGGCCTCTGCG‑C‑GGG‑T‑A‑AC‑CCCGCCCATC‑CGGGGGTT‑TTACCG‑GTTAT‑TAGGCTGGCACAGGCAACTACCGAACCAGGGTCAAACGCCCCAACACGGTCTGCCGTGAAAAAc < 4:6851/135‑1 (MQ=255) cTGGAAATATTTTTGC‑TGTGGAGGCCTCTTCG‑C‑GGG‑T‑T‑AA‑CCCCCCCCAACCGGGGGGT‑TTA‑CC‑GTGAA‑GAGGCTGGCCAAGGCAAAAAACCAACCCCGGTCAAAACCCCCAACACCGTCTGCCGGGAAAAAccc > 2:65455/1‑135 (MQ=255) taaattttttGCGTGGGGGGGCCTCTCCG‑‑‑GGG‑T‑A‑AACCCCCCCCCTA‑GGGGGGTT‑TAACCG‑GTGAT‑GTGGCTGGCACAGGCAACTACCGAACCAGGGTCAAACGCCCCAACACGGTCTGCCGTGAAAAACCCATc < 7:324026/130‑1 (MQ=255) aaTATTTTTGC‑TGTGGCGGCCTCTTCG‑C‑GGG‑T‑T‑AA‑ACCGCCCCGA‑CGGGGGGG‑TTTCCC‑GGGAA‑GAGGGTGGGCCACGCCACTACCGCACCCGGGTCAAACGCCCCCACACGGGATGCCCTGAAAAACCCaaacc > 2:255433/1‑131 (MQ=255) ttttttttGC‑GGGGGGGCCCTC‑TCG‑G‑GGG‑G‑A‑AC‑CCCCCCCCCT‑CGGGGGGT‑TTACCCGGTGAT‑TCGGCGGGCAAAGGCAACTACCGAACCAGGGTCAAACGCCCCAACACGGTCTGCCGTGAAAAACCCATCACCt < 6:212681/133‑1 (MQ=255) tgggggggcgggCTCGTGG‑C‑GGG‑C‑A‑AA‑CCCGCCCCGC‑CGGGGGGT‑TTTCCC‑GTTAATTTGGCTGGAACAGGCAACTACCGAACCAGGGTCAAACGCCCCAACACGGTCTGCCGTGAAAAACCCATCACCTTGccc < 1:174358/129‑1 (MQ=255) gcggcCTCTTCG‑C‑GGG‑T‑A‑AC‑CCCCCCCCTC‑CGGGGGTT‑TTACCG‑TTGAA‑GAGGCTGGCACAGGCAACTACCGAACCAGGGTCAAACGCCCCAACACGGTCTGCCGTGAAAAACCCATCACCTTGCCCAGCGAATcc < 6:116634/136‑1 (MQ=255) gcCGCTTCG‑C‑GGG‑T‑A‑AA‑CCCGCCCCTA‑CGGGGGGT‑TTACCG‑GTGAA‑GAGGCTGGCACAGGCAACTACCGAACCAGGGTCAAACGCCCCAACACGGTCTGCCGTGAAAAACCCATCACCTTGCCCAGCGAATCCAgc < 1:317887/136‑1 (MQ=255) a‑aa‑CCCGCCCCTA‑CGGGGGGT‑TTACCC‑GTGAA‑GAGGCTGGCACAGGCAACTACCGAACCAGGGTCAAACGCCCCAACACGGTCTGCCGTGAAAAACCCATCACCTTGCCCAGCGAATCCAGCGCCAGCAGCGGCTc < 6:163594/136‑1 (MQ=255) aa‑CCCGCCCCTA‑CGGGGGGT‑TTACCC‑GTGAA‑GAGGCTGGCACAGGCAACTACCGAACCAGGGTCAAACGCCCCAACACGGTCTGCCGTGAAAAACCCATCACCTTGCCCAGCGAATCCAGCGCCAGCAGCGGCTc < 1:65455/135‑1 (MQ=255) cTA‑CGGGGGGT‑TTACCC‑GTGAA‑GAGGCTGGCACAGGCAACTACCGAACCAGGGTCAAACGCCCCAACACGGTCTGCCGTGAAAAACCCATCACCTTGCCCAGCGAATCCAGCGCCAGCAGCGGCTCGGCCACCgg > 6:28232/1‑135 (MQ=255) tA‑CGGGGGGT‑TTACCC‑GTGAA‑GAGGCTGGCACAGGCAACTACCGAACCAGGGTCAAACGCCCCAACACGGTCTGCCGTGAAAAACCCATCACCTTGCCCAGCGAATCCAGCGCCAGCAGCGGCTCGGCCACCggg > 2:316452/1‑135 (MQ=255) | ACGCAGACCTGTGAGGGGGTGATTATTGCGAATCAATCGCACTTTCACTCATTACACGGATGCCACGTGCTGTTACCTCACCTGTGCCTGGAAATATTTTTGC‑TGTGGCGGCCTCTTCG‑C‑GGG‑T‑A‑AA‑CCCGCCCCTA‑CGGGGGGT‑TTACCC‑GTGAA‑GAGGCTGGCACAGGCAACTACCGAACCAGGGTCAAACGCCCCAACACGGTCTGCCGTGAAAAACCCATCACCTTGCCCAGCGAATCCAGCGCCAGCAGCGGCTCGGCCACCGGG > ACB143/5222380‑5222649 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |