Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
MC JC	ACB143	5,390,395	(CGCAT)2→1	100%	coding (489‑493/1666 nt)	ligH →	10‑formyltetrahydrofolate synthetase

Missing coverage evidence...
			seq id	start	end	size	←reads	reads→	gene	description
*	*	÷	ACB143	5390395	5390399	5	51 [0]	[0] 51	ligH	10‑formyltetrahydrofolate synthetase

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	ACB143	= 5390394	0 (0.000)	45 (0.780)	35/232	NT	100%	coding (488/1666 nt)	ligH	10‑formyltetrahydrofolate synthetase
	?	ACB143	5390400 =	0 (0.000)					coding (494/1666 nt)	ligH	10‑formyltetrahydrofolate synthetase

GTCGTGCCGATGGAGGACATCAACCTTCACTTCACCGGTGACTTCCATGCCATCACCTCGGCGAACAACCTGCTCGCCGCGCTGATCGACAACCACATCTACTGGGGCAACAAGCTCGGCCTCGATCCGCGCCGCAT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  ACB143/5390258‑5390394 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑cgcatGGCGCCGCGTGCTCGACATGAACGACCGGGCGCTGCGCTCGATCGTCAATTCGCTGGGCGGCGTCTCGAATGGCTATCCGCGCGAGGACGGCTTCGACATCACCGTGGCTTCGGAAGTCATGGCGATCCTG  >  ACB143/5390400‑5390530                                                                                                                                                                                                                                                                              GTCGTGCCGATGGAGGACATCAACCTTCACTTCACCGGTGACTTCCATGCCATCACCTCGGCGAACAACCTGCTCGCCGCGCTGATCGACAACCACATCTACTGGGGCAACAAGCTCGGCCTCGATCCGCGCCGC                                                                                                                                       >  2:189523/1‑135  TCGTGCCGATGGAGGACATCAACCTTCACTTCACCGGTGACTTCCATGCCATCACCTCGGCGAACAACCTGCTCGCCGCGCTGATCGACAACCACATCTACTGGGGCAACAAGCTCGGCCTCGATCCGCGCCGCAT                                                                                                                                     >  4:359679/1‑136       CCGATGGAGGACATCAACCTTCACTTCACCGGTGACTTCCATGCCATCACCTCGGCGAACAACCTGCTCGCCGCGCTGATCGTCAACCACATCTACTGGGGCAACAAGCTCGGCCTCGATCCGCGCCGCATGGCGC                                                                                                                                >  8:345382/1‑136       CCGATGGAGGACATCAACCTTCACTTCACCGGTGACTTCCATGCCATCACCTCGGCGAACAACCTGCTCGCCGCGCTGATCGACAACCACATCTACTGGGGCAACAAGCTCGGCCTCGATCCGCGCCGCATGGCGC                                                                                                                                >  5:286688/1‑136         GATGGAGGACATCAACCTTCACTTCACCGGTGACTTCCATGCCATCACCTCGGCGAACAACCTGCTCGCCGCGCTGATCGACAACCACATCTACTGGGGCAACAAGCTCGGCCTCGATCCGCGCCGCATGGCGCCG                                                                                                                              >  3:31997/1‑136             GAGGACATCAACCTTCACTTCACCGGTGACTTCCATGCCATCACCTCGGCGAACAACCTGCTCGCCGCGCTGATCGACAACCACATCTACTGGGGCAACAAGCTCGGCCTCGATCCGCGCCGCATGGCGCCGCGT                                                                                                                           <  4:319688/135‑1              AGGACATCAACCTTCACTTCACCGGTGACTTCCATGCCATCACCTCGGCGAACAACCTGCTCGCCGCGCTGATCGACAACCACATCTACTGGGGCAACAAGCTCGGCCTCGATCCGCGCCGCATGGCGCCGCGTGC                                                                                                                         >  4:150278/1‑136              AGGACATCAACCTTCACTTCACCGGTGACTTCCATGCCATCACCTCGGCGAACAACCTGCTCGCCGCGCTGATCGACAACCACATCTACTGGGGCAACAAGCTCGGCCTCGATCCGCGCCGCATGGCGCCGCGTGC                                                                                                                         >  5:118483/1‑136                   ATCAACCTTCACTTCACCGGTGACTTCCATGCCATCACCTCGGCGAACAACCTGCTCGCCGCGCTGATCGACAACCACATCTACTGGGGCAACAAGCTCGGCCTCGATCCGCGCCGCATGGCGCCGCGTGCTCGA                                                                                                                     >  8:374785/1‑135                   ATCAACCTTCACTTCACCGGTGACTTCCATGCCATCACCTCGGCGAACAACCTGCTCGCCGCGCTGATCGACAACCACATCTACTGGGGCAACAAGCTCGGCCTCGATCCGCGCCGCATGGCGCCGCGTGCTCGA                                                                                                                     >  2:97402/1‑135                   ATCAACCTTCACTTCACCGGTGACTTCCATGCCATCACCTCGGCGAACAACCTGCTCGCCGCGCTGATCGACAACCACATCTACTGGGGAAACAAGCTCGGCCTCGATCCGCGCCGCATGGCGCCGCGTTCTCGA                                                                                                                     >  3:207723/1‑135                      AACCTTCACTTCACCGGTGACTTCCATGCCATCACCTCGGCGAACAACCTGCTCGCCGCGCTGATCGACAACCACATCTACTGGGGCAACAAGCTCGGCCTCGATCCGCGCCGCATGGCGCCGCGTGCTCGACAT                                                                                                                  >  7:188201/1‑135                        CCTTCACTTCACCGGTGACTTCCATGCCATCACCTCGGCGAACAACCTGCTCGCCGCGCTGATCGACAACCACATCTACTGGGGCAACAAGCTCGGCCTCGATCCGCGCCGCATGGCGCCGCGTGCTCGACATGAA                                                                                                               >  3:166857/1‑136                        CCTTCACTTCACCGGTGACTTCCATGCCATCACCTCGGCGAACAACCTGCTCGCCGCGCTGATCGACAACCACATCTACTGGGGCAACAAGCTCGGCCTCGATCCGCGCCGCATGGCGCCGCGTGCTCGACATGAA                                                                                                               >  8:310423/1‑136                              caTCACCGGTGACTTCCATGCCATCACCTCGGCGAACAACCTGCTCGCCGCGCTGATCGACAACCACATCTACTGGGGCAACAAGCTCGGCCTCGATCCGCGCCGCATGGCGCCGCGTGCTCGACATGAACGACCG                                                                                                         >  8:202328/3‑136                               TTCACCGGTGACTTCCATGCCATCACCTCGGCGAACAACCTGCTCGCCGCGCTGATCGACAACCACATCTACTGGGGCAACAAGCTCGGCCTCGATCCGCGCCGCATGGCGCCGCGTGCTCGACATGAACGACCGG                                                                                                        >  1:191551/1‑136                                TCACCGGTGACTTCCATGCCATCACCTCGGCGATCAACCTGCTCGCCGCGCTGATCGACAACCACATCTACTGGGGCAACAAGCTCGGCCTCGATCCGCGCCGCATGGCGCCGCGTGCTCGACATGAACGACCGGG                                                                                                       >  1:306176/1‑136                                TCACCGGTGACTTCCATGCCATCACCTCGGCGAACAACCTGCTCGCCGCGCTGATCGACAACCACATCTACTGGGGCAACAAGCTCGGCCTCGATCCGCGCCGCATGGCGCCGCGTGCTCGACATGAACGACCGGG                                                                                                       >  1:134142/1‑136                                TCACAGGTGACTTCCATGCCATAACCTCGGCGAACAACCTGCTCGCCGCGCTGATCGACAACAACATCTACTCGGGCAACAAGCTCGGCCTCGATCCGCGCCGCATGGCGCCGCGTGCTCGACATGAACGACCGGG                                                                                                       >  2:119892/1‑136                                  ACCGGTGACTTCCATGCCATCACCTCGGCGAACAACCTGCTCGCCGCGCTGATCGACAACCACATCTACTGGGGCAACAAGCTCGGCCTCGATCCGCGCCGCATGGCGCCGCGTGCTCGACATGAACGACCGGGC                                                                                                      <  8:11889/135‑1                                   CCGGTGACTTCCATGCCATCACCTCGGCGAACAACCTGCTCGCCGCGCTGATCGACAACCACATCTACTGGGGCAACAAGCTCGGCCTCGATCCGCGCCGCATGGCGCCGCGTGCTCGACATGAACGACCGGGCGC                                                                                                    >  2:290939/1‑136                                     GGTGACTTCCATGCCATCACCTCGGCGAACAACCTGCTCGCCGCGCTGATCGACAACCACATCTACTGGGGCAACAAGCTCGGCCTCGATCCGCGCCGCATGGCGCCGCGTGCTCGACATGAACGCCCGGGCGCTG                                                                                                  >  6:11342/1‑136                                       TGACTTCCATGCCATCACCTCGGCGAACAACCTGCTCGCCGCGCTGATCGACAACCACATCTACTGGGGCAACAAGCTCGGCCTCGATCCGCGCCGCATGGCGCCGCGTGCTCGACATGAACGACCGGGCGCTGCG                                                                                                >  4:379746/1‑136                                              CATGCCATCACCTCGGCGAACAACCTGCTCGCCGCGCTGATCGACAACCACATCTACTGGGGCAACAAGCTCGGCCTCGATCCGCGCCGCATGGCGCCGCGTGCTCGACATGAACGACCGGGCGCTGCGCTCGAac                                                                                         >  8:99468/1‑134                                                    agCACGTCGGCGAACAACCTCCTCGCCGCGCTGATCGACAACCCCATCTACTGGGGCAACACGCGCGGCCTCGAGCCGCGCCGCATGGCGCCGCGTGCTCGACATGAACGACCGGGCGCTGCGCTCGATCGTCAAT                                                                                   <  2:146786/134‑1                                                      CACCTCGGCGAACAACCTGCTCGCCGCGCTGATCGACAACCACATCTACTGGGGCAACAAGCTCGGCCTCGATCCGCGCCGCATGGCGCCGCGTGCTCGACATGAACGACCGGGCGCTGCGCTCGATCGTCAATTC                                                                                 >  8:155703/1‑136                                                            GGCGAACAACCTGCTCGCCGCGCTGATCGACAACCACATCTACTGGGGCAACAAGCTCGGCCTCGATCCGCGCCGCATGGCGCCGCGTGCTCGACATGAACGACCGGGCGCTGCGCTCGATCGTCAATTCGCTGGG                                                                           <  8:188201/136‑1                                                              CGAACAACCTGCTCGCCGCGCTGATCGACAACCACATCTACTGGGGCAACAAGCTCGGCCTCGATCCGCGCCGCATGGCGCCGCGTGCTCGACATGAACGACCGGGCGCTGCGCTCGATCGTCAATTCGCTGGGCG                                                                         >  8:64984/1‑136                                                                           CGCCGCGCTGATCGACAACCACATCTACTGGGGCAACAAGCTCGGCCTCGATCCGCGCCGCATGGCGCCGCGTGCTCGACATGAACGACCGGGCGCTGCGCTCGATCGTCAATTCGCTGGGCGGCGTCTCGAATGG                                                            >  1:10426/1‑136                                                                           CGCCGCGCTGATCGACAACCACATCTACTGGGGCAACAAGCTCGGCCTCGATCCGCGCCGCATGGCGCCGCGTGCTCGACATGAACGACCGGGCGCTGCGCTCGATCGTCAATTCGCTGGGCGGCGTCTCGAATGG                                                            >  2:354209/1‑136                                                                            GCCGCGCTGATCGACAACCACATCTACTGGGGCATCAAGCTCGGCCTCGATCCGCGCGGCATGGCGCCGCGTGCTCGACATGAACGACCGGGCGCTGCGCTCGATCGTCAATTCGCTGGGCGGCGTCTCGAATGGC                                                           <  3:256758/136‑1                                                                                             CCACATCTACTGGGGCAACAAGCTCGTCCTCGATCCGCGCCGCATGGCGCCGCGTGCTCGACATGAACGACCGGGCGCTGCGCTCGATCGTCAATTCGCTGGGCGGCGTCTCGAATGGCTATCCGCGCGAGGACGG                                          <  7:221214/136‑1                                                                                                CATCTACTGGGGCAACAAGCTCGGCCTCGATCCGCGCCGCATGGCGCCGCGTGCTCGACATGAACGACCGGGCGCTGAGCTCGATCGTCAATTCGCTGGGCGGCGTCTCGAATGGCTATCCGCGCGAGGACGGCTT                                       >  3:270866/1‑136                                                                                                      CTGGGGCAACAAGCTCGGCCTCGATCCGCGCCGCATGGCGCCGCGTGCTCGACATGAACGACCGGGCGCTGCGCTCGATCGTCAATTCGCTGGGCGGCGTCTCGAATGGCTATCCGCGCGAGGACGGCTTCGA                                    <  4:270866/133‑1                                                                                                      CTGGGGCAACAAGCTCGGCCTCGATCCGCGCCGCATGGCGCCGCGTGCTCGACATGAACGACCGGGCGCTGCGCTCGATCGTCAATTCGCTGGGCGGCGTCTCGAATGGCTATCCGCGCGAGGACGGCTTCG                                     <  1:189523/132‑1                                                                                                         GGGCAACAAGCTCGGCCTCGATCCGCGCCGCATGGCGCCGCGTGCTCGACATGAACGACCGGGCGCTGCGCTCGATCGTCAATTCGCTGGGCGGCGTCTCGAATGGCTATCCGCGCGAGGACGGCTTCGACATC                                <  2:10426/134‑1                                                                                                          GGCAACAAGCTCGGCCTCGATCCGCGCCGCATGGCGCCGCGTGCTCGACATGAACGACCGGGCGCTGCGCTCGATCGTCAATTCGCTGGGCGGCGTCTCGAATGGCTATCCGCGCGAGGACGGCTTCGACATCACC                             >  2:36657/1‑136                                                                                                           GCAACAAGCTCGGCCTCGATCCGCGCCGCATGGCGCCGCGTGCTCGACATGAACGACCGGGCGCTGCGCTCGATCGTCAATTCGCTGGGCGGCGTCTCGAATGGCTATCCGCGCGAGGACGGCTTCGACATCACCG                            >  1:385485/1‑136                                                                                                              ACAAGCTCGGCCTCGATCCGCGCCGCATGGCGCCGCGTGCTCGACATGAACGACCGGGCGCTGCGCTCGATCGTCAATTCGCTGGGCGGCGTCTCGAATGGCTATCCGCGCGAGGACGGCTTCGACATCAC                              >  4:108807/1‑131                                                                                                              ACAAGCTCGGCCTCGATCCGCGCCGCATGGCGCCGCGTGCTCGACATGAACGACCGGGCGCTGCGCTCGATCGTCAATTCGCTGGGCGGCGTCTCGAATGGCTATCCGCGCGAGGACGGCTTCGACATCAC                              <  3:108807/131‑1                                                                                                              ACAAGCTCGGCCTCGATCCGCGCCGCATGGCGCCGCGTGCTCGACATGAACGACCGGGCGCTGCGCTCGATCGTCAATTCGCTGGGCGGCGTCTCGAATGGCTATCCGCGCGAGGACGGCATCGACATCACCGTGG                         >  4:192728/1‑136                                                                                                                 AGCTCGGCCTCGATCCGCGCCGCATGGCGCCGCGTGCTCGACATGAACGACCGGGCGCTGCGCTCGATCGTCAATTCGCTGGGCGGCGTCTCGAATGGCTATCCGCGCGAGGACGGCTTCGACATCACCGTGGCTT                      >  8:247825/1‑136                                                                                                                       GCCTCGATCCGCGCCGCATGGCGCCGCGTGCTCGACATGAACGACCGGGCGCTGCGCTCGATCGTCAATTCGCTGGGCGGCGTCTCGAATGGCTATCCGCGCGAGGACGGCTTCGACATCACCGTGGCTTCGGAAG                <  6:195760/136‑1                                                                                                                        CCTCGATCCGCGCCGCATGGCGCCGCGTGCTCGACATGAACGACCGGGCGCTGCGCTCGATCGTCAATTCGCTGGGCGGCGTCTCGAATGGCTATCCGCGC                                                  <  7:303272/101‑1                                                                                                                        CCTCGATCCGCGCCGCATGGCGCCGCGTGCTCGACATGAACGACCGGGCGCTGCGCTCGATCGTCAATTCGCTGGGCGGCGTCTCGAATGGCTATCCGCGC                                                  >  8:303272/1‑101                                                                                                                         CTCGATCCGCGCCGCATGGCGCCGCGTGCTCGACATGAACGACCGGGCGCTGCGCTCGATCGTCAATTCGCTGGGCGGCGTCTCGAATGGCTATCCGCGCGAGGAGGGCTTCGACATCACCGTGGCTTCGGAAGgc              >  4:6485/1‑134                                                                                                                            GATCCGCGCCGCATGGCGCCGCGTGCTCGACATGAACGACCGGGCGCTGCGCTCGATCGTCAATTCGCTGGGCGGCGTCTCGAATGGCTATCCGCGCGAGGACGGCTTCGACATCACCGTGGCTTCGGAAGTCATG           <  3:204855/136‑1                                                                                                                            GATCCGCGCCGCATGGCGCCGCGTGCTCGACATGAACGACCGGGCGCTGCGCTCGATCGTCAATTCGCTGGGCGGCGTCTCGAATGGCTATCCGCGCGAGGACGGCTTCGACATCACCGTGGCTTCGGAAGTCA             <  7:247825/134‑1                                                                                                                            GATCCGCGCCGCATGGCGCCGCGTGCTCGACATGAACGACCGGGCGCTGCGCTCGATCGTCAATTCGCTGGGCGGCGTCTCGAATGGCTATCCGCGCGAGGACGGCTTCGACATCACCGTGGCTTCGGAAGTCA             <  3:375050/134‑1                                                                                                                                  CGCCGCATGGCGCCGCGTGCTCGACATGAACGACCGGGCGCTGCGCTCGATCGTCAATTCGCTGGGCGGCGTCTCGAATGGCTATCCGCGCGAGGACGGCTTCGACATCACCGTGGCTTCGGAAGTCATGGCGATC     <  2:359895/136‑1                                                                                                                                     CGCATGGCGCCGCGTGCTCGACATGAACGACCGGGCGCTGCGCTCGATCGTCAATTCGCTGGGCGGCGTCTCGAATGGCTATCCGCGCGAGGACGGCTTCGACATCACCGTGGCTTCGGAAGTCATGGCGATCCTG  >  7:186056/1‑136                                                                                                                                     CGCATGGCGCCGCGTGCTCGACATGAACGACCGGGCGCTGCGCTCGATCGTCAATTCGCTGGGCGGCGTCTCGAATGGCTATCCGCGCGAGGACGGCTTCGACATCACCGTGGCTTCGGAAGTCATGGCGATCCTG  >  1:250411/1‑136                                                                                                                                                                                                                                                                              GTCGTGCCGATGGAGGACATCAACCTTCACTTCACCGGTGACTTCCATGCCATCACCTCGGCGAACAACCTGCTCGCCGCGCTGATCGACAACCACATCTACTGGGGCAACAAGCTCGGCCTCGATCCGCGCCGCAT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  ACB143/5390258‑5390394 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑cgcatGGCGCCGCGTGCTCGACATGAACGACCGGGCGCTGCGCTCGATCGTCAATTCGCTGGGCGGCGTCTCGAATGGCTATCCGCGCGAGGACGGCTTCGACATCACCGTGGCTTCGGAAGTCATGGCGATCCTG  >  ACB143/5390400‑5390530

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.