Predicted mutation | |||||||
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evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | ACB143 | 4,135,590 | A→C | 27.0% | Q32P (CAG→CCG) | tgnC → | (Z)‑2‑((N‑methylformamido)methylene)‑5‑hydroxybu tyrolactone dehydrogenase |
Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | ACB143 | 4,135,590 | 0 | A | C | 27.0% | 51.4 / 5.6 | 61 | Q32P (CAG→CCG) | tgnC | (Z)‑2‑((N‑methylformamido)methylene)‑5‑hydroxybu tyrolactone dehydrogenase |
Reads supporting (aligned to +/- strand): ref base A (21/21); new base C (14/4); total (35/26) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 5.25e-02 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
CAAGCCCGCACCCTCACCGAATCCGCCAAGGGGATTGTTGCATGACCCTCGTTCGTTTCCAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCAGGCCTGGGCGCAACTGCCCGACGCCGATGAAGCCGATGTGGAGCTGGCCGTTCAGGCCGCCCAGCGTGCCTTCGACAGCAAGGCGTGGCGCAGCATCACCGCTACCGCCCGCGGCAAGCTGCT > ACB143/4135455‑4135713 | cacgCCCGCACCCTCACCGATTCCGCCAAGGGGTTTGTTGCATGACCCTCGTTCGTTTCCAGATGTGCATCGACGGCCAATGGCGCGTTGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCa < 4:167990/133‑1 (MQ=255) cAAGCCCGCACCCTCACCGAATCCGCCAAGGGGATTGTTGCATGACCCTCGTTCGTTTCCAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCa < 3:336659/136‑1 (MQ=255) cAAGCCCGCACCCTCACCGAATCCGCCAAGGGGATTGTTGCATGACCCTCGTTCGTTTCCAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCa < 8:11053/136‑1 (MQ=255) ccccACCCTCACCTAATCCGCCATGGGGATTTTTGCTTGACCTTCGTGGTTTGCCAGATGTGCATCGACGGCCAATGGCGCTATGCCCAGAGCTCCAAGACCTTCGACAGCGTCAACCCGGCCACCTCCCAGGGCt < 6:34427/133‑1 (MQ=255) cGCACCCTCACCGAATCCGCCAAGGGGATTGTTGCATGACCCTCGTTCGTTTCCAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCACGccag > 6:299212/1‑134 (MQ=255) cGCACCCTCACCGAATCCGCCAAGGGGATTGTTGCATGACCCTCGTTCGTTTCCAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCACGccag > 7:126501/1‑134 (MQ=255) cGCACCCTCACCGAATCCGCCAAGGGGATTGTTGCATGACCCTCGTTCGTTTCCAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCCCCCCCGCCTg > 1:156599/1‑136 (MQ=255) cccTCACCGAATCCGCCAAGGGTTTTGTTGCATGACCCTCGTTCGTTTCCAGTTGTGCATCGACGGCCAATGGCGCTATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCAGGCCTGGgcg < 3:207714/136‑1 (MQ=255) ccTCACCGAATCCGCCAAGGGGATTGTTGCATGACCCTCGTTCGTTTCCAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCAGGCCTGGgcgc > 1:155813/1‑136 (MQ=255) ccTCACCGAATCCGCCAAGGGGATTGTTGCATGACCCTCGTTCGTTTCCAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCCCCCCCGCCAGGgcgc > 7:247496/1‑136 (MQ=255) cGAATCCGCCAAGGGGATTGTTGCATGACCCTCGTTCGTTTCCAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCAGGCCTGGGCGCACCTGc > 2:247952/1‑136 (MQ=255) cGAATCCGCCAAGGGGATTGTTGCATGACCCTCGTTCGTTTCCAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCAGGCCAGGGCGCAACTGc > 2:284888/1‑136 (MQ=255) gAATCCGCCAAGGGGATTGTTGCATGACCCTCGTTCGTTTCCAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCCGGCCTGGGCGCAACTGcc > 8:184475/1‑136 (MQ=255) gCCAAGGGGATTGTTGCATGACCCTCGTTCGTTTCCAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCCGGCCTGGGCGCAACAGcccccccc > 6:299455/1‑131 (MQ=255) gCCAAGGGGATTGTTGCATGACCCTCGTTCGTTTCCAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCAGGCCTGGGCGCAACTGCCCGACGc > 3:59288/1‑136 (MQ=255) aaGGGGTTTGTTGCATGACCCTCGTTCGTTTCCAATTGTCCAGCGCCGGCCAAGGGCGCGAGGCCCAGAGCGGAAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCCGGCCTGGGCGCAACTGCCCGACGCCGa < 3:188147/136‑1 (MQ=255) gggATTTTTGCATGACCCGCGTTCGTTTCCAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCAGGCCTGGGCGCAACTGCCCGACGCCGATGa < 5:385034/136‑1 (MQ=255) gggATTGTTGCATGACCCTCGTTCGTTTCCAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCAGGCCTGGGCGCAACTGCCCGACGCCGATGa < 7:94637/136‑1 (MQ=255) gACCCTCGTTCGTTTCCAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCAGGCCTGGGCGCAACTGCCCGCCGCCGCTGAAGCCGATGTGGAg > 4:132545/1‑136 (MQ=255) gACCCTCGTTCGTTTCCAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCAGGCCAGGGCGCAACTGCCCGACCCCGAAGAAGCCGAAGTGGAg > 8:351262/1‑136 (MQ=255) cccTCGTTCGTTTCCAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCCGGCCTGGGCGCCACTGCCCGCCGCCGCTGAAGCCGATGTGGAGCt > 5:378271/1‑136 (MQ=255) cccTCGTTCGTTTCCAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCCCGCCTGGGCGCCACAGCCCCCAGCCGATGAAGCCGATGTGGAg > 1:367163/1‑134 (MQ=255) cccTCGTTCGTTTCCAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCAGGCCAGGGCGCAACAGCCCGCCGCCGCTGAAGCCGAAGTGGAGCt > 2:320249/1‑136 (MQ=255) cccTCGTTCGTTTCCAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCCCCCACCCCTGGGGGCAACAGCCCGCCGCCCAACAACCCCATGTGGAGCt > 1:144641/1‑136 (MQ=255) tcgttcttTTCCAGTTGTGAATCGACGCCCAAGGGCGCGATGCCCATAGCGCAAACACATTCGACCGCCACACCCCGGCCACCGCCCCGGCCGGGGCGCAACTGCCCGACGCCGATGAAGCCGATGTGGAGCTGGc < 4:363135/136‑1 (MQ=255) tcgttcgtTTCCAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCCCCCCCGCCAGGGCGCCACTGCCCGCCGCCGCTGAAGCCCAGGGGGAGCTGGc > 7:135287/1‑136 (MQ=255) tcgttcgtTTCCAGATGAGCAACGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCACGCCAGGGCGCACCAGACCGCCCCCCATGAAGACGATGTGGAGCTGGc > 7:79077/1‑136 (MQ=255) gttcgttTCCAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCCCCCCCGCCAGGGCGCCACAGCCCCCCGCCGAAGAACCCGAAGTGGACCAGGccc > 5:127634/1‑135 (MQ=255) tcgttTCCAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCCCGCCAGGGCGCCACTGCCCCCCCCCCCTGAAGCCGATGTGGAGCTGGCCGtt > 1:228458/1‑136 (MQ=255) tcgttTCCAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCACGCCAGGGCGCCACAGCCCCCCGCCGATGAAGCCGATGTGGAGCTGGCCGt > 1:27743/1‑135 (MQ=255) tcgttTCCAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCCCCCACGCCGGGGCGCAACTGCCCGACCCCGATGAAGCCGAAGTGGAGCTGGccct > 6:127648/1‑133 (MQ=255) cttttCCAGATGTGCATCTACGGCCAATGGCGCGAGGCCCAGCTCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCAGGCGTGGGCGCAACTGCCCGACGCCGATGAAGCCGATGTGGAGCTGGCCGtt < 4:344312/133‑1 (MQ=255) cgttTCCAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCACGCCTGGGCGCCACTGCCCGACGCCGAAGAAGCCGATGTGGAGCTGGCCGtt > 3:70151/1‑135 (MQ=255) gttTCCAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCAGGCCTGGGCGCAACTGCCCGACGCCGATGAAGCCGATGTGGAGCTGGCCGTTc < 6:103628/135‑1 (MQ=255) tCCAGTTGTGCTGCTCCGGCCATTGGCGCGATGCCCAGAGCGGCATGACCTTCGACACCCTCAACCCGGCCCCCGCCCAGGCCTGGGCGCAACTGCCCGACGCCGATGAAGCCGATGTGGAGCTGGCCGTTCAgg < 2:367163/135‑1 (MQ=255) ccAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCCCGCCAGGGCGCCACTGCCCCCCGCCCCTGAAGCCCATGTGGAGCTGGCCGTTCAGgcc > 6:50383/1‑136 (MQ=255) aTGTGCATCGACGCCCAAGGGCGCGATGCCCAGAGCGCCAACCCCTTCGACAGCCTCACCCCGGCCACCGCCCAGGCCTGGGCGCAACTGCCCGACGCCGATGAAGCCGATGTGGAGCTGGCCGTTCAGGCCGccc < 4:352064/136‑1 (MQ=255) tgtgCAGCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCAGGCCGGGGCGCAACTGCCCGACGCCGATGAAGCCGATGTGGAGCTGGCCGTTCAGGCCGccc < 2:162562/135‑1 (MQ=255) gccATCCACGCCCAAGGGCGCGAGGCCCCGAGCGGCACCCCCTTCCCCCGCCTCACCCCGGCCCCCGCCCAGGCCGGGGCGCAACTGCCCGACGCCGATGAAGCCGATGTGGAGCTGGCCGTTCAGGCCGCCCAg < 4:238904/133‑1 (MQ=255) aTCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCAGGCCTGGGCGGCAATGCCCGCCGCCGCTGAAGCCGAGGTGGAGCTGGCCGTGCCGGCCCCCCcgcggg > 2:393763/1‑134 (MQ=255) cGACGGCCAATGGCGCAATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCAGGCCTGGGCGCAACTGCCCGCCGCCGATGAAGCCGATGTGGAGCTGGCCGTTCAGGCCGCCCCGCGTGcc > 3:90619/1‑136 (MQ=255) cAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCACCCCGGCCACCGCCCAGGCCGGGGCGCAACTGCCCGACGCCGATGAAGCCGATGTGGAGCTGGCCGTTCAGGCCGCCCAGCGTGCCTTCGACa < 3:30505/136‑1 (MQ=255) aaTGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCCCCGCCCAGGCCTGGGCGGACCTGCCCGACGCCGCTGAAGCCGCTGTGGAGCTGGCCGTTCCGGCCGCGCAGCGGGCCTTGGGCAg > 8:375304/1‑136 (MQ=255) tGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCAGGCCTGGGCGCCACTGCCCGCCGCCGCTGAAGCCGATGTGGAGCTGGCCGTTCAGGCCGCCCCGCGTGCCTTCGCCAGCAAGGCGTgg > 2:236114/1‑136 (MQ=255) ggCCCAGCGCGGCAAGACCTTCGACAGCCTCACCCCGGCCACCGCCCCGGCCTGGGCGCAACTGCCCGACGCCGATGAAGCCGATGTGGAGCTGGCCGTTCAGGCCGCCCAGCGTGCCTTCGACAGCAAGGCGTg < 6:127634/134‑1 (MQ=255) ccAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCAGGCCTGGGCGCAACTGCCCGACGCCGATGAAGCCGATGTGGAGCTGGCCGTTCAGGCCGCCCAGCGTGCCTTCGACAGCAAGGCGTGgcgc < 4:112552/136‑1 (MQ=255) cacgagCGCCACCACCTGCGACACCCGAAACCCGGCCACCGCCCCGGCCTGGGCGCAACTGCCCGACGCCGATGAAGCCGATGTGGAGCTGGCCGTTCAGGCCGCCCAGCGTGCCTTCGACAGCAAGGCGTGgcgc < 6:232523/133‑1 (MQ=255) agagCGGCAAGACCTTCGACCGCCTCAACCCGGCCACCGCCCAGGCCTGGGCGCAACTGCCCGACGCCGATGAAGCCGATGTGGAGCTGGCCGTTCAGGCCGCCCAGCGTGCCTTCGACAGCAAGGCGTGGCgcag < 2:259862/136‑1 (MQ=255) agagCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCAGGCCTGGGCGCAACTGCCCGACGCCGATGAAGCCGATGTGGAGCTGGCCGTTCAGGCCGCCCAGCGTGCCTTCGACAGCAAGGCGTGGCgcag < 2:155813/136‑1 (MQ=255) agacCGGCAAGACCTTCGACAGCCTCAACCCGGCCCCCGCCCAGGCCTGGGCGCAACTGCCCGACGCCGATGAAGCCGATGTGGAGCTGGCCGTTCAGGCCGCCCAGCGTGCCTTCGACAGCAAGGCGTGGCgcag < 2:209483/136‑1 (MQ=255) aaGACCTTCGACCGCCTCACCCCGCCCACCGCCCAGGCCTGGGCGCAACTGCCCGACGCCGATGAAGCCGATGTGGAGCTGGCCGTTCAGGCCGCCCAGCGTGCCTTCGACAGCAAGGCGTGGCGCAGCATCACCg < 1:256596/136‑1 (MQ=255) gACCTTCGACAGCCTCAACCCGGCCACCGCCCCGGCCTGGGCGCCACTGCCCGCCGCCGATGAAGCCGATGTGGTGCTGGCCGTTCAGGCCGCCCCGCGTGCCTTCGCCAGCAACGCGTGGCGCAGCATCACCGc > 8:250628/1‑135 (MQ=255) gACCTTCGACAGCCTCAACCCGGCCACCGCCCCGGCCGGGGCGCAACTGCCCGCCGCCGCTGAAGCCGATGTGGAGCTGGCCGTTCAGGCCGCCCAGCGTGCCGTCGACAGCACGGCGGGGCGCCGCATCAcccc > 6:16272/1‑133 (MQ=255) gACCTTCGACAGCCTCAACCCGGCCACCGCCCAGGCCTGGGCGCAACTGCCCGACGCCCATGAAGCCGATGTGGAGCTGGCCGTTAAGGCCGCCCAGCGTGCCTTCGCCAGCAAGGCGTGGCGCCGCATAAcccc > 8:338589/1‑133 (MQ=255) gACCTTCGACAGCCTCAACCCGGCCACCCCCCAAGCCTGGGCGCCACTGCCCCACGCCCATGAAGACGATGTGGAGCTAGCCCTTAAGGCCGCCCACCGTGCCATAGACAGCCAGGCGTTGCGCCGCATAACCGc > 6:336568/1‑135 (MQ=255) cgCCACACCCCGGCCCCCCCCCAGGCCTGGGCGCAACTGCCCGACGCCGATGAAGCCGATGTGGAGCTGGCCGTTCAGGCCGCCCAGCGTGCCTTCGACAGCAAGGCGTGGCGCAGCATCACCGCTACCGCCcgcg < 7:342060/135‑1 (MQ=255) aGCCTCAACCCGGCCACCGCCCAGGCCGGGGCGCAACTGCCCGACGCCGATGAAGCCGATGTGGAGCTGGCCGTTCAGGCCGCCCAGCGTGCCTTCGACAGCAAGGCGTGGCGCAGCATCACCGCTACCGCCcgcg < 8:190487/136‑1 (MQ=255) aGCCTCAACCCGCCCACCGCCATGGCCTGGGCGCAACTGCCCGACGCCGATGAAGCCGATGTGGAGCTGGCCGTTCAGGCCGCCCAGCGTGCCTTCGACAGCAAGGCGTGGCGCAGCATCACCGCTACCGCCcgcg < 1:320249/136‑1 (MQ=255) ccTCACCCCGGCCACCCCCCAGGCCTGGGCGCAACTGCCCGACGCCGATGAAGCCGATGTGGAGCTGGCCGTTCAGGCCGCCCAGCGTGCCTTCGACAGCAAGGCGTGGCGCAGCATCACCGCTACCGCCCGCGGc < 5:305208/136‑1 (MQ=255) cAACCCGGCCACCGCCCCGGCCTGGGCGCAACTGCCCGACGCCGATGAAGCCGATGTGGAGCTGGCCGTTCAGGCCGCCCAGCGTGCCTTCGACAGCAAGGCGTGGCGCCGCATCACCGCTACCCCCCCCGGCAAg > 6:313891/1‑136 (MQ=255) aCCCGGCCACCGCCCCGGCCGGGGCGCAACTGCCCGCCGCCGATGCAGCCGATGTGGAGCTGGCCGTTCAGGCCGCCCAGCGGGCCTTCGACAGCAAGGCGTGGCGCAGCATCCCCGCTACCGCCCGCGGCCAgct > 2:288477/1‑136 (MQ=255) aCCCGGCCACCGCCCAGGCCTGGGCGCAACTGCCCGACGCCGATGAAGCCGATGTGGAGCTGGCCGTTCAGGCCGCCCAGCGTTCCTTCGACAGCAAGGCGTGGCGCAGCATAACCGCTACCGCCCGCGGCAAg > 2:121366/1‑134 (MQ=255) cgcccACCGCCCAGGCCGGGGCGCAACTGCCCGACGCCGATGAAGCCGATGTGGAGCTGGCCGTTCAGGCCGCCCAGCGTGCCTTCGACAGCAAGGCGTGGCGCAGCATCACCGCTACCGCCCGCGGCAAgctgct < 6:250723/133‑1 (MQ=255) cGGCCACCGCCCAGGCCTGGGCGCAACTGCCCGACGCCGATGAAGCCGATGTGGAGCTGGCCGTTCAGGCCGCCCAGCGTGCCTTCGACAGCAAGGCGTGGCGCAGCATCACCGCTACCGCCCGCGGCAAgctgct < 2:148565/136‑1 (MQ=255) | CAAGCCCGCACCCTCACCGAATCCGCCAAGGGGATTGTTGCATGACCCTCGTTCGTTTCCAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCAGGCCTGGGCGCAACTGCCCGACGCCGATGAAGCCGATGTGGAGCTGGCCGTTCAGGCCGCCCAGCGTGCCTTCGACAGCAAGGCGTGGCGCAGCATCACCGCTACCGCCCGCGGCAAGCTGCT > ACB143/4135455‑4135713 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |