| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | ACB143 | 5,386,506 | 1 bp→GT | 100% | intergenic (‑515/+1136) | CJ019_05070 ← / ← nptII | hypothetical protein/neomycin phosphotransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | ACB143 | 5,386,505 | 1 | . | G | 86.4% | 70.0 / 4.7 | 22 | intergenic (‑514/+1137) | CJ019_05070/nptII | hypothetical protein/neomycin phosphotransferase |
| Reads supporting (aligned to +/- strand): ref base . (0/3); new base G (12/7); total (12/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.79e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
| * | ACB143 | 5,386,506 | 0 | C | T | 85.0% | 48.0 / ‑0.0 | 22 | intergenic (‑515/+1136) | CJ019_05070/nptII | hypothetical protein/neomycin phosphotransferase |
| Reads supporting (aligned to +/- strand): ref base C (1/2); new base T (11/7); total (12/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.53e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
CCTTTTTTATTGATAACAAAAATTGATCGAATTCTTTCATTTAAGACCCTAATATGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCCGT‑‑CGT‑‑‑C‑A‑GGATC‑‑ATCC‑GGGCGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTGCGGGTGCCAGGGCGTGCCCTTGGGCT > ACB143/5386410‑5386607 ||| ccTTTTTTATTGATAACAAAAATTGATCGAATTCTTTCATTTAAGACCCTAATATGACATCTCGGCCGGCTGGTCGACGACGGCGGTCTCAGT‑‑GGTG‑‑T‑ACGGTACAAACCC‑CGACGCTAGCAACGCATGAGAAAGc < 5:121379/136‑1 (MQ=255) ttGATAACAAAAATTGATCGAATTCTTTCATTTAAGACCCTAATATGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCAGT‑‑GGTG‑‑T‑ACGGTACAAACCC‑CGACGCTAGCAACGCATGAGAAAGCCCCCGGaa < 4:33657/135‑1 (MQ=255) aaaTTGATCGAATTCTTTCATTTAAGACCCTAATATGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCAGTGGGGT‑‑‑A‑C‑GGTACAAACCC‑CGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTcc < 1:143191/136‑1 (MQ=255) aaaTTGATCGAATTCTTTCATTTAACACCCTAATATGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCAGTGGTGT‑‑‑C‑C‑GGTACAAACCC‑CGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTcc < 7:362509/136‑1 (MQ=255) aTCGAATTCTTTCATTTAAGACCCTAATATGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCAGT‑‑GGTG‑‑T‑ACGGTACAAACCC‑CGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCggggg < 7:212525/135‑1 (MQ=255) aTTAAAGCCCCTAATATGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCAGT‑‑GGT‑‑‑CTACGGTACAAACCC‑CGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTgcgc < 3:286513/136‑1 (MQ=255) aCCCTAATATGACATCTCGGCCGGCTTCGCGACGACGGCGGTCTCAGT‑‑GGTG‑‑T‑ACGGAACAAACCC‑CGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTGCGGGt < 4:288234/136‑1 (MQ=255) ccTAATATGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCAGT‑‑GGTGTAC‑G‑GTACA‑‑AACCACGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTGCGGGTg > 8:31322/1‑135 (MQ=255) atatGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCAGT‑‑GGTG‑‑T‑ACGGTACAAACCC‑CGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTGCGGGTGCCAgg < 4:17800/136‑1 (MQ=255) atatGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCAGT‑‑GGTG‑‑T‑ACGGTACAAACCC‑CGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTGCGGGTGCCAgg < 5:299482/136‑1 (MQ=255) tatGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCAGT‑‑GGTG‑‑T‑ACGGTACAAACCC‑CGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTGCGGGTGCCAggg > 7:249686/1‑136 (MQ=255) tatGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCAGT‑‑GGTG‑‑T‑ACGGTACAAACCC‑CGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTGCGGGTGCCAggg > 6:90161/1‑136 (MQ=255) atGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCAGT‑‑GGTG‑‑T‑ACGGTACAAACCC‑CGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATAACCTTACGGGGGCTTTTTTATTGCGCTGCGGGTGCCAGGGc > 3:210869/1‑136 (MQ=255) ctcGGCCGGCTTGTCGACGACGGCGGTCTCAGT‑‑GGTG‑‑T‑ACGGTACAAACCC‑CGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTTCGCTGCGGGTGCCAGGGCGTGCCCt > 6:82164/1‑136 (MQ=255) ctcGGCCGGCTTGTCGACGACGGCGGTCTCAGT‑‑GGTG‑‑T‑ACGGTACAAACCC‑CGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTGCGGGTGCCAGGGCGTGCCCt > 1:75963/1‑136 (MQ=255) ctcGGCCGGCTTGTCGACGACGGCGGTCTCAGT‑‑GGTG‑‑T‑ACGGTACAAACCC‑CGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTGCGGGTGCCAGGGCGTGCCCt > 7:171506/1‑136 (MQ=255) ctcGGCCGGCTTGTCGACGACGGCGGTCTCAGT‑‑GGTG‑‑T‑ACGGTACAAACCC‑CGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTGCGGGTGCCAGGGCGTGCCCt > 4:124443/1‑136 (MQ=255) ctcGGCCGGCTTGTCGACGACGGCGGTCTCAGT‑‑GGTG‑‑T‑ACGGTACAAACCC‑CGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTGCGGGTGCCAGGGCGTGCCCt > 3:266685/1‑136 (MQ=255) cggccggcTTGTCGACGACGGCGGTCTCAGT‑‑GGTG‑‑T‑ACGGTACAAACCC‑CGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTGCGGGTGCCAGGGCGTGCCCTTg > 7:363491/1‑136 (MQ=255) ggccggcTTGTCGACGACGGCGGTCTCAGT‑‑GGTG‑‑T‑ACGGTACAAACCC‑CGACGCTAGCAACGCATGAGAAAGc > 4:354843/1‑73 (MQ=255) ggccggcTTGTCGACGACGGCGGTCTCAGT‑‑GGTG‑‑T‑ACGGTACAAACCC‑CGACGCTAGCAACGCATGAGAAAGc < 3:354843/73‑1 (MQ=255) cggcTTGTCGACGACGGCGGTCTCAGT‑‑GGTG‑‑T‑ACGGTACAAACCC‑CGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCATTTTTATTGCGCTGCGGGTGCCAGGGCGTGCCCTTGGGCt > 2:145798/1‑136 (MQ=255) ||| CCTTTTTTATTGATAACAAAAATTGATCGAATTCTTTCATTTAAGACCCTAATATGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCCGT‑‑CGT‑‑‑C‑A‑GGATC‑‑ATCC‑GGGCGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTGCGGGTGCCAGGGCGTGCCCTTGGGCT > ACB143/5386410‑5386607 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |