Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	ACB143	5,760,598	(A)6→5	coding (781/834 nt)	CJ019_05400 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	ACB143	5,760,593	0	A	.	97.9%	221.1 / ‑1.2	48	coding (776/834 nt)	CJ019_05400	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (1/0);  new base . (25/22);  total (26/22)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.45e-01

AGCTGCAAGCGCGCGAACAGGACCAGGCCGACCTGGCCAAGGTCTTGAAGACCATCGAGGAAACCCTCGCCCGCCAGGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGGAAAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAACCGCCGAAAAAGGCCCGTACCACCCTCGGGCCGATGGTTTCCAGCGATGGC  >  ACB143/5760461‑5760721                                                                                                                                     |                                                                                                                                 aGCTGCAAGCGCGCGAACAGGACCAGGCCGACCTGGCCAAGGTCTTGAAGACAATCGAGGAAACCATCGCCCGCCAGGCCCGTTAAGCTGAAGAGGCACGCAAGAAGTCCCTGCTGGCGCAGCAGGAAGCGGaaa                                                                                                                                >  1:31421/1‑135 (MQ=255)      cacgcgcgcGAACAGGCCCAGGCCGACCTGGCCAAGGTCTTTAAGACCATCGAGGAAACCCTCGCCCGCCAGGCCCGTGAAGCTCAAGAGGCACCCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGGaaaaacg                                                                                                                            <  4:258455/131‑3 (MQ=255)                 aCAGGACCAGGCCGCCCTGGCCAAGGTCTTGAAGACCATCGAGGAAACCCTCGCCCGCCAGGCCCGTGAAGCTGAATAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGaa                                                                                                              <  4:267992/136‑1 (MQ=255)                         cgcccGACCTGGCCAAGGTCTTGAAGACAATCGAGGACACCCTCGCCCTCCAGGCCCGTGAAGCTCAAGCGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGTAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGgc                                                                                                      <  4:133004/133‑1 (MQ=255)                             cGACCTGGCCAAGGTCTTGAAGACCATCGAGGAAACCCTCGCCCGCCAGGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGgccgcc                                                                                                  >  6:287456/1‑136 (MQ=255)                             cGACCTGGCCAAGGTCTTGAAGACCATCGAGGAAACCCTCGCCCGCCAGGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGgccgcc                                                                                                  >  5:7287/1‑136 (MQ=255)                             cGACCTGGCCAAGGTCTTGAAGAACATCGAGGAAACACTCGCCCGCCAGGCACGTGAAGCTGCAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCACGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGgccgcc                                                                                                  >  5:152909/1‑136 (MQ=255)                              gACCTGGCCAAGGTCTTGAAGACCATCGAGGAAACCCTCGCCCGCCAGGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGgccgccg                                                                                                 >  1:82690/1‑136 (MQ=255)                              gACCTGGCCAAGGTCTTGAAGACCATCGAGGAAACCCTCGCCCGCCAGGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGgccgccg                                                                                                 >  6:158521/1‑136 (MQ=255)                               aCCTGGCCAAGGTCTTGAAGACCATCGCGGAAACCCTCGCCCGCCAGGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGgccgccgc                                                                                                <  2:44971/136‑1 (MQ=255)                                  tGGCCAAGGTCTTGAAGACCATCGAGGAAACCCTCGCCCGCCAGGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCcgccgccgc                                                                                             >  8:26081/1‑136 (MQ=255)                                  tGGCCAAGGTCTTGAAGACCATCGAGGAAACCCTCGCCCGCCAGGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCcgccgccgc                                                                                             >  4:376356/1‑136 (MQ=255)                                           tCTTGAAGACCATCGCGGAACCCCTCGCCCGCCAGGCCCGTGAAGCTGAAGCGGCACGCAAGACGGCCCTGCTGGCGCCGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGa                                                                                    <  7:144271/136‑1 (MQ=255)                                            cTTGAAGACCATCGAGGAAACACTCGCCCGCAAGGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGAc                                                                                   >  3:160735/1‑136 (MQ=255)                                                 agcccATCGAGGAAACCCTCGCCCGCCAGGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGc                                                                              <  3:144930/133‑1 (MQ=255)                                                 aGACCATCGAGGAAACCCTCGCCCGCCAGGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGc                                                                              <  4:292221/136‑1 (MQ=255)                                                 aGACCATCGAGGAAACCCCCGCCCGCCAGGCCCGTCAAGCGTAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGc                                                                              <  3:86899/136‑1 (MQ=255)                                                 aGACCAGCGCGCAACCCCGCGCCCGCCCGGCCCGTGACGCTCACGCGCCCCCCACGACGGCCCTGCTGGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGc                                                                              <  1:362081/136‑1 (MQ=255)                                                   aCCATCGAGGAAACCCTCGCCCGCCAGGCCCGTGAAGCTGAAGAGCCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGccc                                                                            <  2:257888/136‑1 (MQ=255)                                                    ccATCGCGCAAACCCCCGCCCGCCAGGCCCGTGAAGCTGACGAGGCACGCAACAAGGCCCTGCTGGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCg                                                                           <  8:147960/136‑1 (MQ=255)                                                           ggAAACCCTCGCCCGCCAGGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCt                                                                    <  8:105458/136‑1 (MQ=255)                                                             aaccccTCGCCCGCCAGGCCCGTGAAGCGGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGc                                                                  <  8:340916/133‑1 (MQ=255)                                                             aaaCCCTCGCCCGCCAGGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGTAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGc                                                                  <  3:292675/136‑1 (MQ=255)                                                             aaaCCCTCGCCCGCCAGGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGc                                                                  <  3:36294/136‑1 (MQ=255)                                                             aaaCCCTCGCCCGCCAGGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGc                                                                  <  6:175254/136‑1 (MQ=255)                                                               aCCCTCGCCCGCCAGGCCCGTGAAGCTGAAGAGGCCCGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGCCCGGGCCCGTGAGCCTGCag                                                                >  6:181953/1‑136 (MQ=255)                                                                        cgccAGGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTgg                                                       >  4:302861/1‑136 (MQ=255)                                                                                     aaGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAACCGCCGaaaaa                                          <  2:221400/136‑1 (MQ=255)                                                                                      aGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGG‑AAAAACgccgcc                                                                                                                      >  1:123022/1‑59 (MQ=255)                                                                                      aGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGG‑AAAAACgccgcc                                                                                                                      <  2:123022/59‑1 (MQ=255)                                                                                      aGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAACCGCCGAAAAAg                                         >  6:275907/1‑136 (MQ=255)                                                                                      aGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAACCGCCGAAAAAg                                         >  1:191787/1‑136 (MQ=255)                                                                                      aGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAACCGCCGAAAAAg                                         >  8:192271/1‑136 (MQ=255)                                                                                      aGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAACCGCCGAAAAAg                                         >  3:114075/1‑136 (MQ=255)                                                                                      aGCTGAACAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAACCGCCGAAAAAg                                         >  3:14001/1‑136 (MQ=255)                                                                                           aaGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGCCCGGGCCCGTGAGCCTGCAGCGCCAGTGGAACCGCCGAAAAAGGCCCg                                    >  7:313318/1‑136 (MQ=255)                                                                                            agagGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAACCGCCGAAAAAGGCCCg                                    <  8:178521/135‑1 (MQ=255)                                                                                              agGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAACCGCCGAAAAAGGCCCGTac                                 >  8:33546/1‑136 (MQ=255)                                                                                                     cAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGTAAAAAAC‑ACGACAGCAGGAAGCGCTGGCAGCCGCCGCTGCCAGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAACCGCCGAAAAAGGCCCGTACCACCCTc                          >  2:249315/1‑136 (MQ=255)                                                                                                     cAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGG‑AAAACCGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAACCGCCGAAAAAGGCCCGTACCACCCTc                          >  4:187403/1‑136 (MQ=255)                                                                                                        gaagGCCCTGCTGGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAACCGCCGAAAAAGGCCCGTACCACCCTCggg                       >  8:287960/1‑136 (MQ=255)                                                                                                         aagGCCCTGCTGGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAACCGCCGAAAAAGGCCCGTACCACCCTCGGGc                      <  1:41501/136‑1 (MQ=255)                                                                                                                   tgGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGCGCCTGAAGAGCCAGTGGAACCGCCGAAAAAGGCCCGTACCACCCGCGGGCCGCGGGTTTc            >  2:189062/1‑136 (MQ=255)                                                                                                                     gcgcAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGCCCGGGCCCGTGAGCCTGCAGAGCCAGTGGAACCGCCGAAAAAGGCCCGTACCACCCTCGGGCCGATGGTTTc            >  3:353404/1‑134 (MQ=255)                                                                                                                     gcgcAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAACCGCCGAAAAAGGCCCGTACCACCCTCGGGCCGATGGTTTc            >  5:348337/1‑134 (MQ=255)                                                                                                                     gcgcAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAACCGCCGAAAAAGGCCCGTACCACCCTCGGGCCGATGGTTTc            >  1:48090/1‑134 (MQ=255)                                                                                                                           cagGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAACCGCCGAAAAAGGCCCGTACCACCCTCGGGCCGATGGTTTCCAGCGATg    <  1:69602/136‑1 (MQ=255)                                                                                                                            agGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAACCGCCGAAAAAGGCCCGTACCACCCTCGGGCCGATGGTTTCCAGCGATgg   >  5:5939/1‑136 (MQ=255)                                                                                                                             ggAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAACCGCCGAAAAAGGCCCGTACCACCCTCGGGCCGATGGTTTCCAGCGATGgc  <  7:42974/136‑1 (MQ=255)                                                                                                                             ggAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAACCGCCGAAAAAGGCCCGTACCACCCTCGGGCCGATGGTTTCCAGCGATGgc  <  7:197358/136‑1 (MQ=255)                                                                                                                                     |                                                                                                                                 AGCTGCAAGCGCGCGAACAGGACCAGGCCGACCTGGCCAAGGTCTTGAAGACCATCGAGGAAACCCTCGCCCGCCAGGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGGAAAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAACCGCCGAAAAAGGCCCGTACCACCCTCGGGCCGATGGTTTCCAGCGATGGC  >  ACB143/5760461‑5760721

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑