Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	ACB143	3,541,597	(G)6→7	coding (80/195 nt)	CJ019_03358 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	ACB143	3,541,591	1	.	G	98.4%	233.9 / ‑3.6	63	coding (74/195 nt)	CJ019_03358	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base . (0/1);  new base G (40/22);  total (40/23)
Fisher's exact test for biased strand distribution p-value = 3.65e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TTCAACTGGCTGTAGCGCAGTTTGCCGGGTGTAACTTTCATGGCTACAGGTCACGGGAACATGGGGAAGCTCACTTTTTGTACTGCGCAGTTGGGTCGTAGAGGCTTTCTCCAGAGCTGTGTCTTGCTCGGCGC‑GGG‑GGGCGCGGTGTTCGGCGCCTCGGCGGCCGTTGCGGGCAAGACGGTCGAAAAGCCCGGGTTCGTGGTAATCAACGGTTGGGTATTGCCGTCCCGTTACTTTCGGAACGCGTCAGCATGATCAAGGACT  >  ACB143/3541458‑3541721                                                                                                                                       |                                                                                                                                    ttCAACTGGCTGTAGCGCAGTTTGCCGGGTGTAACTTTCATGGCTACAGGTCACGGGAACATGGGGAAGCTCACTTTTTGTACTGCGCAGTTTGGTCGTAGAGGCTTTCTCCAGAGCTGTGTCTTGCTCGGcgc‑g                                                                                                                                    <  7:334079/135‑1 (MQ=255)  tCAACTGGCTGTAGCGCAGTTTGCCGGGTGTAACTTTCATGGCTACAGGTCACGGGAACATGGGGAAGCTCACTTTTTGTACTGCGCAGTTGGGTCGTAGAGGCTTTCTCCAGAGCTGTGTCTTGCTCGGCGC‑ggg                                                                                                                                  >  6:190502/1‑136 (MQ=255)  tCAACTGGCTGTAGCGCAGTTTGCCGGGTGTAACTTTCATGGCTACAGGTCACGGGAACATGGGGAAGCTCACTTTTTGTACTGCGCAGTTGGGTCGTAGAGGCTTTCTCCAGAGCTGTGTCTTGCTCGGCGC‑ggg                                                                                                                                  >  2:93996/1‑136 (MQ=255)  tCAACTGGCTGTAGCGCAGTTTGCCGGGTGTAACTTTCATGGCTACAGGTCACGGGAACATGGGGAAGCTCACTTTTTGTACTGCGCAGTTGGGTCGTAGAGGCTTTCTCCAGAGCTGTGTCTTGCTCGGCGC‑ggg                                                                                                                                  >  4:133447/1‑136 (MQ=255)            gTAGCGCAGTTTGCCGGGTGTAACTTTCATGGCTACAGGTCACGGGAACATGGGGAAGCTCACTTTTTGTACTGCGCAGTTGGGTCGTAGAGGCTTTCTCCAGAGCTGTGTCTTGCTAGGCGCGGGG‑GGGCGCGgt                                                                                                                        >  2:177998/1‑136 (MQ=255)                        gCCGGGTGTAACTTTCATGGCTACAGGTCACGGGAACATGGGGAAGCTCACTTTTTGTACTGCGCAGTTGGGTCGTAGAGGCTTTCTCCAGAGCTGTGTCTTGCTCGGCGCGGGG‑GGGCGCGGTGTTCGGCGCCTc                                                                                                            >  5:209043/1‑136 (MQ=255)                        gCCGGGTGTAACTTTCATGGCTACAGGTCACGGGAACATGGGGAAGCTCACTTTTTGTACTGCGCAGTTGGGTCGTAGAGGCTTTCTCCAGAGCTGTGTCTTGCTCGGCGCGGGG‑GGGCGCGGTGTTCGGCGCCTc                                                                                                            >  3:184776/1‑136 (MQ=255)                               gtAACTTTCATGGCTACAGGTCACGGGAACATGGGGAAGCTCACTTTTTGTACTGCGCAGTTGGGTCGTAGAGGCTTTCTCCAGAGCTGTGTCTTGCTCGGCGCGGGG‑GGGCGCGGTGTTCGGCGCCTCGGCGGcc                                                                                                     <  8:212381/136‑1 (MQ=255)                                tAACTTTCATGGCTACAGGTCACGGGAACATGGGGAAGCTCACTTTTTGTACTGCGCAGTTGGGTCGTAGAGGCTTTCTCCAGAGCTGTGTCTTGCTCGGCGCGGGG‑GGGCGCGGTGTTCGGCGCCTCGGCGGcc                                                                                                     <  1:145487/135‑1 (MQ=255)                                tAACTTTCATGGCTACAGGTCACAGGAACATGGGGAAGCTCACTTTTTGTACTGCGCAGTTGGGTCGTAGAGGCTTTCTCCAGAGCTGTGTCTTGCTCGGCGCGGGG‑GGGCGCGGTGTTCGGCGCCTCGGCGGcc                                                                                                     <  7:269357/135‑1 (MQ=255)                                  aCTTTCATGGCTACAGGTCACGGGAACATGGGGAAGCTCACTTTTTGTACTGCGCAGTTGGGTCGTAGAGGCTTTCTCCAGAGCTGTGTCTTGCTCGGCGCGGGG‑GGGCGCGGTGTTCGGCGCCTCGGCGGCCGtt                                                                                                  >  4:141486/1‑136 (MQ=255)                                                     aCGGGAACATGGGGAAGCTCACTTTTTGTACTGCGCAGTTGGGTCGTAGAGGCTTTCTCCAGAGCTGTGTCTTGCTCGGCGCGGGG‑GGGCGCGGTGTTCGGCGCCTCGGCGGCCGTTGCGGGCAAGACGGTCGaa                                                                                >  8:97796/1‑135 (MQ=255)                                                      cGGGAACATGGGGAAGCTCACTTTTTGTACTGCGCAGTTGGGTCGTAGAGGCTTTCTCCAGAGCTGTGTCTTGCTCGGCGCGGGG‑GGGCGCGGTGTTCGGCGCCTCGGCGGCCGTTGCGGGCAAGACGGTCGaaaa                                                                              <  1:93996/136‑1 (MQ=255)                                                         gAACATGGGGAAGCTCACTTTTTGTACTGCGCAGTTGGGTCGTAGAGGCTTTCTCCAGAGCTGTGTCTTGCTCGGCGCGGGG‑GGGCGCGGTGTTCGGCGCCTCGGCGGCCGTTGCGGGCAAGACGGTCGAAAAGcc                                                                           >  4:116955/1‑136 (MQ=255)                                                          aaCATGGGGAAGCTCACTTTTTGTACTGCGCAGTTGGGTCGTAGAGGCTTTCTCCAGAGCTGTGTCTTGCTCGGCGCGGGG‑GGGCGCGGTGTTCGGCGCCTCGGCGGCCGTTGCGGGCAAGACGGTCGAAAAGccc                                                                          >  1:10095/1‑136 (MQ=255)                                                          aaCATGGGGAAGCTCACTTTTTGTACTGCGCAGTTGGGTCGTAGAGGCTTTCTCCAGAGCTGTGTCTTGCTCGGCGCGGGG‑GGGCGCGGTGTTCGGCGCCTCGGCGGCCGTTGCGGGCAAGACGGTCGAAAAGccc                                                                          >  6:55742/1‑136 (MQ=255)                                                            cATGGGGAAGCTCACTTTTTGTACTGCGCAGTTGGGTCGTAGAGGCTTTCTCCAGAGCTGTGTCTTGCTCGGCGCGGGG‑GGGCGCGGTGTTCGGCGCCTCGGCGGCCGTTGCGGGCAAGACGGTCGAAAAGCCCgg                                                                        <  6:349962/136‑1 (MQ=255)                                                                      gtCACATTTTGTACTGCTCAGTTGGGTCGTAGAGGCTTTCTCCAGAGCTGTGTCTTGCTCGGCGCGGGG‑GGGCGCGGTGTTCGGAGGGTCGGCGGGCGTTGCGGGCAAGTCGGTCTAAAAGCCCGGGTTCGTGGTa                                                              <  8:144574/135‑1 (MQ=255)                                                                      cTCACTTTTTGTACTGCGCAGTTGGGTCGTAGAGGCTTTCTCCAGAGCTGTGTCTTGCTCGGCGCGGGG‑GGGCGCGGTGTTCGGCGCCTCGGCGGCCGTTGCGGGCAAGACGGTCGAAAAGCCCGGGTTCGTGGt                                                               >  7:106963/1‑135 (MQ=255)                                                                      cTCACTTTTTGTACTGCGCAGTTGGGTCGTAGAGGCTTTCTCCAGAGCTGTGTCTTGCTCGGCGCGGGG‑GGGCGCGGTGTTCGGCGCCTCGGCGGCCGTTGCGGGCAAGACGGTCGAAAAGCCCGGGTTCGTGGTa                                                              <  3:141486/136‑1 (MQ=255)                                                                       tCACTTTTTGTACTGCGCAGTTGGGTCGTAGAGGCTTTCTCCAGAGCTGTGTCTTGCTCGGCGCGGGG‑GGGCGCGGTGTTCGGCGCCTCGGCGGCCGTTGCGGGCAAGCCGGTCGAAAAGCCCGGGTTCGTGGt                                                               >  5:6648/1‑134 (MQ=255)                                                                         aCTTTTTGTACTGCGCAGTTGGGTCGTAGAGGCTTTCTCCAGAGCTGTGTCTTGCTCGGCGCGGGG‑GGGCGCGGTGTTCGGCGCCTCGGCGGCCGTTGCGGGCAAGACGGTCGAAAAGCCCGGGTTCGTGGTAAt                                                            >  5:89949/1‑135 (MQ=255)                                                                         aCTTTTTGTACTGCGCAGTTGGGTCGTAGAGGCTTTCTCCAGAGCTGTGTCTTGCTCGGCGCGGGG‑GGGCGCGGTGTTCGGCGCCTCGGCGGCCGTTGCGGGCAAGACGGTCGAAAAGCCCGGGTTCGTGGTAAt                                                            <  7:86027/135‑1 (MQ=255)                                                                         aCTTTTTGTACTGCGCAGTTGGGTCGTAGAGGCTTTCTCCAGAGCTGTGTCTTGCTCGGCGCGGGG‑GGGCGCGGTGTTCGGCGCCTCGGCGGCCGTTGCGGGCAAGACGGTCGAAAAGCCCGGGTTCGTGGTAAt                                                            >  8:98225/1‑135 (MQ=255)                                                                         aCTTTTTGTACTGCGCAGTTGGGTCGTAGAGGCTTTCTCCAGAGCTGTGTCTTGCTCGGCGCGGGG‑GGGCGCGGTGTTCGGCGCCTCGGCGGCCGTCGCGGGCAAGACGGTCGAAAAGCCCGGGTTCGTGGTAAt                                                            <  8:83377/135‑1 (MQ=255)                                                                              ttGTACTGCGCAGTTGGGTCGTAGAGGCTTTCTCCAGAGCTGTGTCTTGCTCGGCGCGGGG‑GGGCGCGGTGTTCGGCGCCTCGGCGGCCGTTGCGGGCAAGACGGTCGAAAAGCCCGGGTTCGTGGTAATCAACgg                                                      >  7:152529/1‑136 (MQ=255)                                                                              ttGTACTGCGCAGTTGGGGCGTAGAGGCTTTCTCCAGAGCTGTGTCTTGCTCGGCGCGGGG‑GGGCGCGGGGTTCGGCGCCTCGGCGGCCGTTGCGGGCAAGACGGTCGAAAAGCCCGGGTTCGTGGTAATCAACg                                                       <  5:227817/135‑1 (MQ=255)                                                                                        cAGTTGGGTCGTAGAGGCTTTCTCCAGAGCTGTGTCTTGCTCGGCGCGGGG‑GGGCGCGGTGTTCGGCGCCTCGGCGGCCGTTGCGGGCa                                                                                           <  4:204808/89‑1 (MQ=255)                                                                                        cAGTTGGGTCGTAGAGGCTTTCTCCAGAGCTGTGTCTTGCTCGGCGCGGGG‑GGGCGCGGTGTTCGGCGCCTCGGCGGCCGTTGCGGGCa                                                                                           >  3:204808/1‑89 (MQ=255)                                                                                        cAGTTGGGTCGTAGAGGCTTTCTCCAGAGCTGTGTCTTGCTCGGCGCGGGG‑GGGCGCGGTGTTCGGCGCCTCGGCGGCCGTTGCGGGCAAGACGGTCGAAAAGCCCGGGTTCGTGGTAATCAACGGTTGGGTATTg                                            >  3:367622/1‑136 (MQ=255)                                                                                        cAGTTGGGTCGTAGAGGCTTTCTCCAGAGCTGTGTCTTGCTCGGCGCGGGG‑GGGCGCGGTGTTCGGCGCCTCGGCGGCCGTTGCGGGCAAGACGGTCGAAAAGCCCGGGTTCGTGGTAATCAACGGTTGGGTATTg                                            >  7:321495/1‑136 (MQ=255)                                                                                        cAGTTGGGTCGTAGAGGCTTTCTCCAGAGCTGTGTCTTGCTCGGCGCGGGG‑GGGCGCGGTGTTCGGCGCCTCGGCGGCCGTTGCGGGCAAGACGGTCGAAAAGCCCGGGTTCGTGGTAATCAACGGTTGGGTATTg                                            >  1:346301/1‑136 (MQ=255)                                                                                        cAGTTGGGTCGTAGAGGCTTTCTCCAGAGCTGTGTCTTGCTCGGCGCGGGG‑GGGCGCGGTGTTCGGCGCCTCGGCGGCCGTTGCGGGCAAGACGGTCGAAAAGCCCGGGTTCGTGGTAATCAACGGTTGGGTATTg                                            >  1:192639/1‑136 (MQ=255)                                                                                        cAGTTGGGTCGTAGAGGCTTTCTCCAGAGCTGTGTCTTGCTCGGCGCGGGG‑GGGCGCGGTGTTCGGCGCCTCGGCGGCCGTTGCGGGCAAGACGGTCGAAAAGCCCGGGTTCGTGGTAATCAACGGTTGGGTATTg                                            >  1:166638/1‑136 (MQ=255)                                                                                        cAGTTGGGTCGTAGAGGCTTTCTCCAGAGCTGTGTCTTGCTCGGCGCGGGG‑GGGCGCGGTGTTCGGCGCCTCGGCGGCCGTTGCGGGCAAGACGGTCGAAAAGCCCGGGTTCGTGGTAATCAACGGTTGGGTATTg                                            >  1:108040/1‑136 (MQ=255)                                                                                        cAGTTGGGTCGTAGAGGCTTTCTCCAGAGCTGTGTCTTGCTCGGCGCGGGG‑GGGCGCGGTGTTCGGCGCCTCGGCGGCCGTTGCGGGCAAGACGGTCGAAAAGCCCGGGTTCGTGGTAATCAACGGTTGGGTATTg                                            >  3:26170/1‑136 (MQ=255)                                                                                        cAGTTGGGTCGTAGAGGCTTTCTCCAGAGCTGTGTCTTGCTCGGCGCGGGG‑GGGCGCGGTGTTAGGCGCCTCGGCGGCCGTTGCGGGCAAGACGGTAGAAAAGCCCGGGTTCGTGGTAATCAACGGTTGGGTATTg                                            >  1:216817/1‑136 (MQ=255)                                                                                              ggTCGTAGAGGCTTTCTCCAGAGCTGTGTCTTGCTCGGCGCGGGG‑GGGCGCGGTGTTCGGCGCCTCGGCGGCCGTTGCGGGCAAGACGGTCGAAAAGCCCGGGTTCGTGGTAATCAACGGTTGGGTATTGCCGTcc                                      >  7:112474/1‑136 (MQ=255)                                                                                              ggTCGTAGAGGCTTTCTCCAGAGCTGTGTCTTGCTCGGCGCGGGG‑GGGCGCGGTGTTCGGCGCCTCGGCGGCCGTTGCGGGCAAGACGGTCGAAAAGCCCGGGTTCGTGGTAATCAACGGTTGGGTATTGCCGTcc                                      >  1:201735/1‑136 (MQ=255)                                                                                                   tAGAGGCTTTCTCCAGAGCTGTGTCTTGCTCGGCGCGGGG‑GGGCGCGGTGTTCGGCGCCTCGGCGGCCGTTGCGGGCAAGACGGTCGAAAAGCCCGGGTTCGTGGTAATCAACGGTTGGGTATTGCCGTCCCGTTa                                 >  5:278800/1‑136 (MQ=255)                                                                                                         cTTTCTCCAGAGCTGTGTCTTGCTCGGCGCGGGG‑GGGCGCGGTGTTCGGCGCCTCGGCGGCCGTTGCGTGCAAGACGGTCGAAAAGCCCGGGTTCGTGGTAATCAACGGTTGGGTATTGCCGTCCCGTTACTTTCg                           <  5:255582/136‑1 (MQ=255)                                                                                                         cTTTCTCCAGAGCTGTGTCTTGCTCGGCGC‑GGGTGGGCGCGGTGTTCGGCGCCGCGGCGGCCGTTGCGGGCAAGACGGTCGAAAAGCCCGGGTTCGTGGTAATCAACGGTTGGGTATTGCCGTCCCGTTACTTTCg                           <  8:256508/136‑1 (MQ=255)                                                                                                           ttCTCCAGAGCTGTGTCTTGCTCGGCGCGGGG‑GGGCGCGGTGTTCGGCGCCTCGGCGGCCGTTGCGGGCAAGACGGTCGAAAAGCCCGGGTTCGTGGTAATCAACGGTTGGGTATTGCCGTCCCGTTACTTTCgg                          >  3:232566/1‑135 (MQ=255)                                                                                                             ctcCAGAGCTGTGTCTTGCTCGGCGCGGGG‑GGGCGCGGTGTTCGGCGCCTCGGCGGCCGTTGCGGGCAAGACGGTCGAAAAGCCCGGGTTCGTGGTAATCAACGGTTGGGTATTGCCGTCCCGTTACTTTCGGAAc                       >  8:14166/1‑136 (MQ=255)                                                                                                             ctcCAGAGCTGTGTCTTGCTCGGCGCGGGG‑GGGCGCGGTGTTCGGCGCCTCGGCGGCCGTTGCGGGCAAGACGGTCGAAAAGCCCGGGTTCGTGGTAATCAACGGTTGGGTATTGCCGTCCCGTTACTTTCGGAAc                       >  2:11270/1‑136 (MQ=255)                                                                                                             ctcCAGAGCTGTGTCTTGCTCGGCGCGGGG‑GGGCGCGGTGTTCGACGCCTCGGCGGCCGTTGCGGGCAAGACGGTCGAAAAGCCCGGGTTCGTGGTAATCAACGGTTGGGTATTGCCGTCCCGTTACTTTCGGAAc                       >  8:145476/1‑136 (MQ=255)                                                                                                                cAGAGCTGTGTCTTGCTCGGCGCGGGG‑GGGCGCGGTGTTCGGCGCCTCGGCGGCCGTTGCGGGCAAGACGGTCGAAAAGCCCGGGTTCGTGGTAATCAACGGATGGGTATTGCCGTCCCGTTACTTTCGGAAcg                      <  1:157849/134‑1 (MQ=255)                                                                                                                   agCTGTGTCTTGCTCGGCGCGGGG‑GGGCGCGGTGTTCGGCGCCTCGGCGGCCGTTGCGGTCAAGACGGTCGAAAAGCCCGGGTTCGTGGTAATCAACGGTTGGGTATTGCCGTCCCGTTACTTTCGGAACGCGTCa                 <  8:120203/136‑1 (MQ=255)                                                                                                                   agCTGTGTCTTGCTCGGCGCGGGG‑GGGCGCGGTGTTCGGCGCCTCGGCGGCCGTTGCGGGCAAGACGGTCGAAAAGCCCGGGTTCGTGGTAATCAACGGTTGGGTATTGCCGTCCCGTTACTTTCGGAACGCGTCa                 <  1:239999/136‑1 (MQ=255)                                                                                                                   agCTGTGTCTTGCTCGGCGCGGGG‑GGGCGCGGTGTTCGGCGCCTCGGCGGCCGTTGCGGGCAAGACGGTCGAAAAGCCCGGGTTCGTGGTAATCAACGGTTGGGTATTGCCGTCCCGTTACTTTCGGAACGCGTCa                 <  5:190502/136‑1 (MQ=255)                                                                                                                   agCTGTGTCTTGCTCGGCGCGGGG‑GGGCGCGGGGTTCGGCGCCGCGGCGGCCGTTGCGGGCAAGACGGTCGAAAAGCCCGGGTTCGTGGTAATCAACGGTTGGGTATTGCCGTCCCGTTACTTTCGGAACGCGTCa                 <  5:39488/136‑1 (MQ=255)                                                                                                                      tgtgTCTTGCTCGGCGCGGGG‑GGGCGCGGTGTTCGGCGCCTCGGCGGCCGTTGCGGGCAAGACGGTCGAAAAGCCCGGGTTCGTGGTAATCAACGGTTGGGTATTGCCGTCCCGTTACTTTCGGAACGCGTCAGCa              >  3:163703/1‑136 (MQ=255)                                                                                                                      tgtgTCTTGCTCGGCGCGGGG‑GGGCGCGGTGTTCGGCGCCTCGGCGGCCGTTGCGGGCAAGACGGTCGAAAAGCCCGGGTTCGTGGTAATCAACGGTTGGGTATTGCCGTCCCGTTACTTTCGGAACGCGTCAGCa              >  2:122137/1‑136 (MQ=255)                                                                                                                          tCTTGCTCGGCGCGGGG‑GGGCGCGGTGTTCGGCGCCTCGGCGGCCGTTGCGGGCAAGACGGTCGAAAAGCCCGGGTTCGTGGTAATCAACGGTTGGGTATTGCCGTCCCGTTACTTTCGGAACGCGTCAGCATGAt          <  3:354972/136‑1 (MQ=255)                                                                                                                          tCTTGCTCGGCGCGGGG‑GGGCGCGGTGTTCGGCGCCTCGGCGGCCGTTGCGGGCAAGACGGTCGAAAAGCCCGGGTTCGTGGTAATCAACGGTTGGGTATTGCCGTCCCGTTACTTTCGGAACGCGTCAGCATGAt          <  2:201735/136‑1 (MQ=255)                                                                                                                          tCTTGCTCGGCGCGGGG‑GGGCGCGGTGTTCGGCGCCTCGGCGGCCGTTGCGGGCAAGACGGTCGAAAAGCCCGGGTTCGTGGTAATCAACGGTTGGGTATTGCCGTCCCGTTACTTTCGGAACGCGTCAGCATGAt          <  5:343678/136‑1 (MQ=255)                                                                                                                          tCTTGCTCGGCGCGGGG‑GGGCGCGGTGTTCGGCGCCTCGGCGGCCGTTGCGGGCAAGACGGTCGAAAAGCCCGGGTTCGTGGTAATCAACGGTTGGGTATTGCCGTCCCGTTACTTTCGGAACGCGTCAGCATGAt          <  4:184776/136‑1 (MQ=255)                                                                                                                           cTTGCTCGGCGCGGGG‑GGGCGCGGTGTTCGGCGCCTCGGCGGCCGTTGCGGGCAAGACGGTc                                                                                   >  4:265221/1‑62 (MQ=255)                                                                                                                           cTTGCTCGGCGCGGGG‑GGGCGCGGTGTTCGGCGCCTCGGCGGCCGTTGCGGGCAAGACGGTc                                                                                   <  3:265221/62‑1 (MQ=255)                                                                                                                           cTTGCTCGGCGCGGGG‑GGGCGCGGTGTTCGGCGCCTCGGCGGCCGTTGCGGGCAAGACGGTCGAAAAGCCCGGGTTCGTGGTAATCAACGGTTGGGTATTGCCGTCCCGTTACTTTCGGAACGCGTCAGCATGAt          >  3:214675/1‑135 (MQ=255)                                                                                                                           cTTGCTCGGCGCGGGG‑GGGCGCGGTGTTCGGCGCCTCGGCGGCCGTTGCGGGCAAGACGGTCGAAAAGCCCGGGTTCGTGGTAATCAACGGTTGGGTATTGCCGTCCCGTTACTTTCGGAACGCGTCAGCATGAt          >  8:194215/1‑135 (MQ=255)                                                                                                                           cTTGCTCGGCGCGGGG‑GGGCGCGGTGTTCGGCGCCTCGGCGGCCGTTGCGGGCAAGACGGTCGAAAAGCCCGGGTTCGTGGTAATCAACGGTTGGGTATTGCCGTCCCGTTACTTTCGGAACGCGTCAGCATGAt          >  1:106511/1‑135 (MQ=255)                                                                                                                           cTTGCTCGGCGCGGGG‑GGGCGCGGTGTTCGGCGCCTCGGCGGCCGTTGCGGGCAAGACGGTCGAAAAGCCCGGGTTCGTGGTAATCAACGGTTGGGTATTGCCGTCCCGTTACTTTCGGAACCCGTCAGCATGATc         >  8:304499/1‑136 (MQ=255)                                                                                                                           cTTGCTCGGCGCGGGG‑GGGCGCGGTGTTCGGCGCCTCGGCGGCCGTTGCGGGCAAGACGGTCGAAAAGCCCGGGTTCGTGGTAATCAACGGGTGGGTATTGCCGTCCGGTTACGTTCGGCACGCGTCCGCCTGAt          >  3:192497/1‑135 (MQ=255)                                                                                                                                tCGGCGCGGGG‑GGGCGCGGTGTTCGGCGCCTCGGCGGCCGTTGCGGGCAAGACGGTCGAAAAGCCCGGGTTCGTGGTAATCAACGGTTGGGTATTGCCGTCCCGTTACTTTCGGAACGCGTCAGCATGATCAAGGa    >  8:120202/1‑136 (MQ=255)                                                                                                                                tCGGCGCGGGG‑GGGCGCGGTGTTCGGCGCCTCGGCGGCCGTTGCGGGCAAGACGGTCGAAAAGCCCGGGTTCGTGGTAATCAACGGTTGGGTATTGCCGTCCCGTTACTTTCGGAACGCGTCAGCATGATCAAGGa    >  4:87687/1‑136 (MQ=255)                                                                                                                                  ggCGCGGGG‑GGGCGCGGTGTTCGGCGCCTCGGCGGCCGTTGCGGGCAAGACGGTCGAAAAGCCCGGGTTCGTGGTAATCAACGGTTGGGTATTGCCGTCCCGTGACTTTCGGAACGCGTCAGCATGATCAAGGACt  >  1:36789/1‑136 (MQ=255)                                                                                                                                       |                                                                                                                                    TTCAACTGGCTGTAGCGCAGTTTGCCGGGTGTAACTTTCATGGCTACAGGTCACGGGAACATGGGGAAGCTCACTTTTTGTACTGCGCAGTTGGGTCGTAGAGGCTTTCTCCAGAGCTGTGTCTTGCTCGGCGC‑GGG‑GGGCGCGGTGTTCGGCGCCTCGGCGGCCGTTGCGGGCAAGACGGTCGAAAAGCCCGGGTTCGTGGTAATCAACGGTTGGGTATTGCCGTCCCGTTACTTTCGGAACGCGTCAGCATGATCAAGGACT  >  ACB143/3541458‑3541721

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑