| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | ACB143 | 6,043,196 | 0 | C | A | 37.0% | 101.0 / 48.2 | 100 | A37S (GCC→TCC) | puuD_3 | Gamma‑glutamyl‑gamma‑aminobutyrate hydrolasePuuD |
| Reads supporting (aligned to +/- strand): ref base C (38/25); new base A (0/37); total (38/62) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.04e-11 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CTGGGGGCGCCATTGTAATGATGCGGCTCGATATTTGAAGGCGAGCCGGTAAAAAC‑AATGCCGTGCAGGCGAGCCAGCAGCGCTTGCGTGTCGCTGCCGCCATCACGGGCCGGC‑AAGATCAGTGGCAAGCCGGCAAA‑GCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTGCGCTCATGGGCTTACACCGTGTGCAGGTAC > ACB143/6043063‑6043327 | cTGGGGGCGCCATTGTAATGATGCGGCTCGATATTTGAAGGCGAGCCGGTAAAAAC‑AATGCCGTGCAGGCGAGCCAGCAGCGCTTGCGTGTCGCTGCCGCCATCACGGGCCGGC‑AAGATCAGTGGCAAGCCGGCa > 2:290036/1‑135 (MQ=255) cgcCATTGTAATGATGCGGCTCTATATTTGAAGGCGAGCCGGTAAAAAC‑AATGCCGTGCAGGCGAGCCAGCAGCGCTTGCGTGTCGCTGCCGCCATCACGGGCCGGC‑AAGATCAGTGGCAAGCCGGAAAA‑gccggc < 3:281012/136‑1 (MQ=255) gcCATTGTAATGATGCGGCTCGATATTTGAAGGCGAGCCGGTAAAAAC‑AATGCCGTGCAGGCGAGCCAGCAGCGCTTGCGTGTCGCTGCCGCCATCACGGGCCGGC‑AAGATCAGTGGCAAGCCGGCAAA‑gccggcc > 7:268811/1‑136 (MQ=255) aTTGTAATGATGCGGCTCGATATTTGAAGGCGAGCCGGTAAAAAC‑AATGCCGTGCAGGCGAGCCAGCAGCGCTTGCGTGTCGCTGCCGCCATCACGGGCCGGC‑AAGATCAGTGGCAAGCCGGCAAA‑GCCGgccgc > 4:310717/1‑135 (MQ=255) tgatgCGGCTCGATATTTGAAGGCGAGCCGGTAAAAAC‑AATGCCGTGCAGGCGAGCCAGCAGCGCTTGCGTGTCGCTGCCGCCATCACGGGCCGGC‑AAGATCAGTGGCAAGCCGGCAAA‑GCCGGCCGCCTCGACa > 2:222042/1‑135 (MQ=255) ttggggCTCGATTTTTCAAGGC‑TTCCGGTAAAAACAAATGCCGTGCTGGCGAGCCAGCCGCGCTTGCGTGTCGCTGCCGCCATCCCGGGCCGCC‑ATCAACTGGGCCAAGCGGGAAAC‑GCCGGCCGCCTCGACATa < 4:332717/131‑1 (MQ=255) atgCGGCTCGATATTTGAAGGCGAGCCGGTAAAAAC‑AATGCCGTGCAGGCGAGCCAGCAGCGCTTGCGTGTCGCTGCCGCCATCACGGGCCGGC‑AAGATCAGTGGCAAGCCGGCAAA‑GCCGGCCGCCTCGACATAc > 1:78928/1‑136 (MQ=255) cGGCTCGATATTTGAAGGCGAGCCGGTAAAAAC‑AATGCCGTGCAGGCGAGCCAGCAGCGCTTGCGTGTCGCTGCCGCCATCACGGGCCGGC‑AAGATCAGTGGCAAGCCGGCAAA‑GCCGGCCGCCTCGACATACTTg > 6:48269/1‑136 (MQ=255) cGGCTCGATATTTGAAGGCGAGCCGGTAAAAAC‑AATGCCGTGCAGGCGAGCCAGCAGCGCTTGCGTGTCGCTGCCGCCATCACGGGCCGGC‑AAGATCAGTGGCAAGCCGGCAAA‑GCCGGCCGCCTCGACATACTTg > 5:3107/1‑136 (MQ=255) cGGCTCGATATTTGAAGGCGAGCCGGTAAAAAC‑AATGCCGTGCAGGCGAGCCAGCAGCGCTTGCGTGTCGCTGCCGCCATCACGGGCCGGC‑AAGATCAGTGGCAAGCCGGCAAA‑GCCGGCCGCCTCGACATACTTg > 2:352281/1‑136 (MQ=255) cGGCTCGATATTTGAAGGCGAGCCGGTAAAAAC‑AATGCCGTGCAGGCGAGCCAGCAGCGCTTGCGTGTCGCTGCCGCCATCACGGGCCGGC‑AAGATCAGTGGCAAGCCGGCAAA‑GCCGGCCGCCTCGACATACTTg > 6:177433/1‑136 (MQ=255) cGGCTCGATATTTGAAGGCGAGCCGGTAAAAAC‑AATGCCGTGCAGGCGAGCCAGCAGCGCTTGCGTGTCGCTGCCGCCATCACGGGCCGGC‑AAGATCAGTGGCAAGCCGGCAAA‑GCCGGCCGCCTCGACATACTTg > 4:44178/1‑136 (MQ=255) cGGCTCGATATTTGAAGGCGAGCCGGTAAAAAC‑AATGCCGTGCAGGCGAGCCAGCAGCGCTTGCGTGTCGCTGCCGCCATCACGGGCCGGC‑AAGATCAGTGGCAAGCCGGCAAA‑GCCGGCCGCCTCGACATACTTg > 8:225802/1‑136 (MQ=255) cGGCTCGATATTTGAAGGCGAGCCGGTAAAAAC‑AATGCCGTGCAGGCGAGCCAGCAGCGCTTGCGTGTCGCTGCCGCCATCACGGGCCGGC‑AAGATCAGTGGCAAGCCGGCAAA‑GCAGGCCGCCTCGACATACTTg > 1:232241/1‑136 (MQ=255) cTCGATATTTGAAGGCGAGCCGGTAAAAAC‑AATGCCGTGCAGGCGAGCCAGCAGCGCTTGCGTGTCGCTGCCGCCATCACGGGCCGGC‑AAGATCAGTGGCAAGCCGGCAAA‑GCCGGCCGCCTCGACATACTTGTct > 1:98314/1‑135 (MQ=255) cTCGATATTTGAAGGCGAGCCGGTAAAAAC‑AATGCCGTGCAGGCGAGCCAGCAGCGCTTGCGTGTCGCTGCCGCCATCACGGGCCGGC‑AAGATCAGTGGCAAGCCGGCAAA‑GCCGGCCGCCTCGACATACTTGTCg > 5:281907/1‑136 (MQ=255) cTCGATATTTGAAGGCGAGCCGGTAAAAAC‑AATGCCGTGCAGGCGAGCCAGCAGCGCTTGCGTGTCGCTGCCGCCATCACGGGCCGGC‑AAGATCAGTGGCAAGCCGGCAAA‑GCCGGCCGCCTCGACATACTTGTCg > 8:326121/1‑136 (MQ=255) cGATATTTGAAGGCGAGCCGGTAAAAAC‑AATGCCGTGCAGGCGAGCCAGCAGCGCTTGCGTGTCGCTGCCGCCATCACGGGCCGGC‑AAGATCAGTGGCAAGCCGGCAAA‑GCCGGCCGCCTCGACATACTTGTCGcc > 6:327733/1‑136 (MQ=255) cGATATTTGAAGGCGAGCCGGTAAAAAC‑AATGCCGTGCAGGCGAGCCAGCAGCGCTTGCGTGTCGCTGCCGCCATCACGGGCCGGC‑AAGATCAGTGGCAAGCCGGCAAA‑GCCGGCCGCCTCGACATACTTGTCGcc > 1:70551/1‑136 (MQ=255) cGATATTTGAAGGCGAGCCGGTAAAAAC‑AATGCCGTGCAGGCGAGCCAGCAGCGCTTGCGTGTCGCTGCCGCCATCACGGGCCGGC‑AAGATCAGTGGCAAGCCGGCAAA‑GCCGGCCGCCTCGACATACTTGTCGcc > 5:178483/1‑136 (MQ=255) agggCGCGCCGGTAAAAAC‑AATGCGGTGCCGGCGACCCAGCAGCGCTGGGGTGGCGCGGCCGCAATCACGGGCCGGA‑AAGATCAGTGCCACGCCGCAAAA‑GCCGGCCGCCTCGACATACTTGTCGCCGACCgtgt < 4:152820/133‑1 (MQ=255) acggCGAGCCGGTAAAAAC‑AATGCCGTGCAGGCGAGCCAGCAGCGCTTGCGTGTCGCTGCCGCCATCACGGGCCGGC‑AAGATCAGTGGCAAGCCGGCAAA‑GCCGGCCGCCTCGACATACTTGTCGCCGACCgtgt < 1:60796/133‑1 (MQ=255) aaGGCGAGCCGGTAAAAAC‑AATGCCGTGCAGGCGAGCCAGCAGCGCTTGCGTGTCGCTGCCGCCATCACGGGCCGGC‑AAGATCAGTGGCAAGCCGGCAAA‑GCCGGCCGCCTCGACATACTTGTCGCCGACCgtgt < 7:225802/135‑1 (MQ=255) aaGGCGAGCCGGTAAAAAC‑AATGCCGTGCAGGCGAGCCAGCAGCGCTTGCGTGTCGCTGCCGCCATCACGGGCCGGC‑AAGATCAGTGGCAAGCCGGCAAA‑GCCGGCCGCCTCGACATACTTGTCGCCGACCGtgtg > 4:38064/1‑136 (MQ=255) aGGCGAGCGGGTAAAAAC‑AATGCCGTGCAGGCGAGCCAGCAGCGCTTGCGTGTCGCTGCCGCCATCACGGGCGGGA‑AAGATCAGTGGCAAGCCGGCAAA‑GCGGGCCGCCTCGACATACTTGTCGCCGACCGTGTGc < 5:135343/136‑1 (MQ=255) aGGCGAGCCGGTAAAAAC‑AATGCCGTGCAGGCGAGCCTGCAGCGCTTGCGTGTCGCTGCCGCCATCACGGGCCGGC‑AAGATCAGTGGCAAGCCGGAAAA‑GCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGc < 3:250704/136‑1 (MQ=255) ggCGAGCCGGTAAAAAC‑AATGCCGTGCAGGCGAGCCAGCAGCGCTTGCGTGTCGCTGCCGCCATCACGGGCCGGC‑AAGATCAGTGGCAAGCCGGCAAA‑GCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGc > 7:208405/1‑135 (MQ=255) gAGCCGGTAAAAAC‑ACTGCCGTGCAGGCGAGCCAGCCGCGCTTGGGTGGCGCTGCCGCCATCACGGGCCGCC‑AAGATCAGTGGCAAGCCGGCAAA‑GCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCgagg < 6:110966/136‑1 (MQ=255) gCCGGTAAAAAC‑AATGCCGTGCAGGCGAGCCAGCAGCGCTTGCGTGTCGCTGCCGCCATCACGGGCCGGC‑AAGATCAGTGGCACGCCGGCAAA‑GCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCgaggag > 7:314356/1‑136 (MQ=255) cgtaaaaaaC‑ATTGCCGTGCAGGCGAGCCAGCAGCGCTGGCGTGTCGCTGCCGCCATCACGGGCCGGC‑AAGATCAGTGGAAAGCCGGAAAA‑GCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGtt < 2:19839/132‑1 (MQ=255) cGGTAAAAAC‑AATGCCGTGCAGGCGAGCCAGCAGCGCTTGCGTGTCGCTGCCGCCATCACGGGCCGGC‑AAGATCAGTGGCAAGCCGGCAAA‑GCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGt > 7:162685/1‑135 (MQ=255) gtaaaaaaC‑AAGGCCGTGCAGGCGAGCCAGCAGCGCTTGCGGGTCGCTGCCGCCATCAGGGGCCGGC‑AAGATCAGTGCCAAGCCGGAAAA‑GCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGttc < 6:175527/133‑1 (MQ=255) cGTGCAGGCGAGCCAGCAGCGCTTGCGTGTCGCTGCCGCCATCACGGGCCGGC‑AAGATCAGTGGCAAGCCGGCAAA‑GCCGGCCGCCTCGACATACTTGTTGCCGACCGGGGGCGAGGAGTTCTTCCCTACctgctg > 5:21209/1‑136 (MQ=255) cGTGCAGGCGAGCCAGCAGCGCTTGCGTGTCGCTGCCGCCATCACGGGCCGGC‑AAGATCAGTGGCAAGCCGGCAAA‑GCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACctgct > 6:323503/1‑135 (MQ=255) gctggCTAGCCAGCCGCGCTGGCGGGGCGCGGCCGCCATCACGGGCCGGA‑AAGATCCGGGGCACGCCGGAAAA‑GCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACg < 6:325548/133‑1 (MQ=255) gccggCTAGCCAGCCGCGCTTGGGTGGCGCTGCCGCCAGCACGGGCGGGC‑AAGATCCGTGGCAAGCCGGAAAA‑GCGGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACg < 8:267251/133‑1 (MQ=255) gCAGGCTAGCCAGCAGCGCTTGCGTGTCGCTGCCGCCTACAGGGGCCGGA‑AATATCAGTGGCAAGCCGTAAAA‑GCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACg < 2:285039/136‑1 (MQ=255) gCAGGCGAGCCCGCCGCGCTTGCGTGGCGCTGCCGCCACCACGGGCGGGA‑AAGATCAGTGCCAAGCCGCAAAA‑GCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACg < 5:172182/136‑1 (MQ=255) gCAGGCGAGCCAGCAGCGCTTGCGTGTCGCTGCCGCCATCACGGGCCGGC‑AAGATCAGGGGCAAGCCGGCAAA‑GCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACg < 8:3592/136‑1 (MQ=255) gCAGGCGAGCAAGCTGCGGTTGTGGTTGTGTGCGGCCATAAGGGGCCGTC‑AATATCTGTGGCACGCGGGCATT‑GCCGCCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACg < 2:279684/136‑1 (MQ=255) gCAGGCGAGACAGCCGCGGTTGCGTGTCGCTGCCGCCATCCGGGGCCGGC‑AAGATCAGTGGCAAGCCGGAAAA‑GCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACg < 3:97162/136‑1 (MQ=255) cccggCGAGCCAGCCGCGATGGGTGGTCGGGGCCGCCATCCCGGGCGGGC‑ATGATCAGTGCCACGCCGGAAAA‑GCGGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACg < 8:350249/133‑1 (MQ=255) aGGCGAGCCAGCAGCGCTTGCGTGTCGCTGCCGCCATCACGGGCCGGC‑AAGATCAGTGGCAAGCCGGCAAA‑GCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACgg > 4:26755/1‑135 (MQ=255) gcagcGCTTGCGTGTCGCTGCCGCCATCACGGGCCGGC‑AAGATCAGTGGCAAGCCGGCAAA‑GCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTg > 2:242886/1‑134 (MQ=255) gcagcGCTTGCGTGTCGCTGCCGCCATCACGGGCCGGC‑AAGATCAGTGGCAAGCCGGCAAA‑GCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTg < 1:242886/134‑1 (MQ=255) gcagcGCTTGCGTGTCGCTGCCGCCATCACGGGCCGGC‑AAGATCAGTGGCAAGCCGGCAAA‑GCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGac > 7:103295/1‑136 (MQ=255) gcagcGCTTGCGTGTCGCTGCCGCCATCACGGGCCGGC‑AAGATCAGTGGCAAGCCGGCAAA‑GCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGa > 2:37143/1‑135 (MQ=255) gcagcGCTTGCGAGTCGCTGCCGCCATCACGGGCCGGC‑AAGATCAGTGGCAAGCCGGCAAA‑GCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGa > 3:294151/1‑135 (MQ=255) gCGTGTCGCTGCCGCCATCACGGGCCGGC‑AATATCAGTGGAAAGCCGGAAAA‑GCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCaa < 8:32868/136‑1 (MQ=255) ggtgtCGGTGCCGCAATCTGGGGCGGGA‑ATTATCTGTGGCACGCGGCAAAA‑GCCGCCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCaa < 1:161303/134‑1 (MQ=255) tgtCGCTGCCGCCATCACGGGCCGGC‑AAGATCAGTGGCAAGCCGGCAAA‑GCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAgg < 6:178483/136‑1 (MQ=255) tgccGCTGCCGCCATCACGGGCCGGC‑AAGATCAGTGGCAAGCCGGCAAA‑GCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAgg < 2:240591/133‑1 (MQ=255) gggcccTGCCGCCATCACGGGCGGCC‑AAGATCTGTGGCAAGCGGGCAAA‑GCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAgg < 4:104545/131‑1 (MQ=255) cTGCCGCCATCACGGGCCGGC‑AAGATCAGTGGCAAGCCGGCAAA‑GCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCg > 4:126618/1‑136 (MQ=255) ccgccATCACGGGCCGGC‑AAGATCAGTGGCAAGCCGGCAAA‑GCCGGCCGCCTCGCCATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCAt > 4:168441/1‑136 (MQ=255) ccATCACGGGCCGGC‑AAGATCAGTGGCAAGCCGGCAAA‑GCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGAAGGCAGGCGCTGCCACCGATCAAAGGGACCGCATTTg > 1:298380/1‑136 (MQ=255) tccctggCCGGC‑AATATCCTGGGCACCCCGCCAAA‑GCCGGCCGCCGCTACATACTTGTCGCCGACCGTGTGAGAGTAGTTCTTCCCTACCTGCTGACGGCAGGCGATGACACCGATCAAATGGACCTCATTTgcgc < 2:314238/131‑1 (MQ=255) tcccGGGCCGGC‑AAGATCAGTGGCAAGCCGGAAAA‑GCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTgcgc < 2:85337/133‑1 (MQ=255) tCAGGGGCGGGA‑AAGATCAGTGGCAAGCCGTAAAA‑GCCGCCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGTGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTgcgc < 1:119669/136‑1 (MQ=255) tCAGGGGCCGGC‑AATATCATGGGCAACCGGGCAAC‑GCCGTCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTgcgc < 7:342986/136‑1 (MQ=255) tCAGGGGCCGGC‑AAGATCAGTGGCAAGCCGGCAAA‑GCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTgcgc < 6:188650/136‑1 (MQ=255) tCAGGGGCCGGC‑AAGATCAGTGGCAAGCCGGAAAA‑GCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTgcgc < 4:131025/136‑1 (MQ=255) tCAGGGGCCGGA‑AATATCCGGGGCAAGCCGGAAAC‑GCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTgcgc < 4:7930/136‑1 (MQ=255) tCAGGGGCCGCC‑AAGATCAGTGGAACGCCGGCAAA‑CCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTgcgc < 3:74427/136‑1 (MQ=255) tCACGGGCCGGC‑AATATCTGGGCCACCCCGGAAAC‑GCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTgcgc < 6:191672/136‑1 (MQ=255) tCACGGGCCGGC‑AAGATCCGGGGCAAGCCGGCAAA‑GCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTgcgc < 1:81673/136‑1 (MQ=255) tCACGGGCCGGC‑AAGATCAGTGGCACGCCGGCAAA‑GCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTgcgc < 6:81738/136‑1 (MQ=255) tCACGGGCCGGC‑AAGATCAGTGGCAAGCCGGCAAA‑GCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTgcgc < 6:365721/136‑1 (MQ=255) tCACGGGCCGGC‑AAGATCAGTGGCAAGCCGGCAAA‑GCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTgcgc < 6:173508/136‑1 (MQ=255) tCACGGGCCGGC‑AAGATCAGTGGCAAGCCGGAAAA‑GCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTgcgc < 8:16362/136‑1 (MQ=255) tCACGGGCCGGC‑AAGATCAGTGGCAAGCCGGAAAA‑GCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTgcgc < 3:126618/136‑1 (MQ=255) tCACGGGCCGGC‑AAGATCAGTGGCAAGCCGGAAAA‑GCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTgcgc < 3:208513/136‑1 (MQ=255) tCACGGGCCGGC‑AAGATCAGTGGCAAGCCGGAAAA‑GCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTgcgc < 4:135737/136‑1 (MQ=255) tCACGGGCCGGC‑AAGATCAGTGCCAAGCCGGAAAA‑GCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTgcgc < 2:20326/136‑1 (MQ=255) tCACGGGCCGGC‑AAGAGCCGTGCCACGCCGGAAAA‑GCGGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTgcgc < 6:78936/136‑1 (MQ=255) tCACGGGCCGGA‑AAGATCTGGGGCAAGCCGGCAAA‑GCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGCTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTgcgc < 6:323036/136‑1 (MQ=255) gcaggggCGGGA‑AAGATCTGTGGCAAGCCGCAAAA‑GCGGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTgcgc < 5:314668/132‑1 (MQ=255) acaggggCCGGA‑AAGATCTGGGGCACG‑CGGCAAACGCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTgcgc < 3:38064/132‑1 (MQ=255) acACGGGCGGCC‑AAGATCTGTGGAACGCCTCAAAC‑GCCGCCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTgcgc < 1:153993/135‑1 (MQ=255) taCGGGGCGGCAAAGATCTGGGGCACGCCGGAAAC‑GCGGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTgcgc < 7:321417/135‑1 (MQ=255) cACGGGCCGGC‑AAGATCAGTGGCAAGCGGGCAAA‑GCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTgcgc < 6:83106/135‑1 (MQ=255) aCGGGCCGGA‑AAGATCCGGGGCAAGCCGGAAAA‑GCGGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTGCGCTc < 4:274168/136‑1 (MQ=255) cGGGCCGGC‑AAGATCAGTGGCAAGCCGGCAAA‑GCCGGCCGCCTCGACATACTTGTCGCCGCCCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGCCACCGATCAAAGGGACCGCCTTTGCGCTCa > 6:27922/1‑136 (MQ=255) ccGGC‑AAGATCAGTGGCAAGCCGGCAAA‑GCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCAGCTGACGGCAGGCGCTGCCACCGATCAAAGGGACCGCATTTGCGCTCATggg > 4:299482/1‑136 (MQ=255) cc‑AACATCAGTGCCAACCCGGAAAC‑GCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTGCGCTCATGGGCtt < 4:258925/135‑1 (MQ=255) atgATCCGTGCCACGCCGGAAAC‑GCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTGCGCTCATGGGCTTac < 6:3107/134‑1 (MQ=255) ataTCAGTGGCACGCCGGAAAT‑GCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTGCGCTCATGGGCTTaca < 1:230182/134‑1 (MQ=255) aTCAGTGCCATGCCGGAAAA‑GCCGCCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTGCGCTCATGGGCTTACAcc < 2:328156/136‑1 (MQ=255) cattgtcAAGCCGGCAAA‑GCCGGCCGCCTCGAGATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTGCGCTCATGGGCTTACACCgt < 4:1312/130‑1 (MQ=255) aggggCAAGCCGGCAAA‑GCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTGCGCTCATGGGCTTACACCgt < 8:41785/132‑1 (MQ=255) aggggCAAGCCGGCAAA‑GCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTGCGCTCATGGGCTTACACCgt < 8:41787/132‑1 (MQ=255) aGTGGCAAGCCGGCAAA‑GCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTGCGCTCATGGGCTTACACCgt < 8:86220/135‑1 (MQ=255) tGGCAAGCCGGAAAA‑GCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTGCGCTCATGGGCTTACACCgtgt < 7:207734/135‑1 (MQ=255) tGGCAAGCCGGAAAA‑GCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTGCGCTCATGGGCTTACACCgt < 2:93100/133‑1 (MQ=255) gggCAAGCCGGAAAA‑GCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTGCGCTCATGGGCTTACACCgtgt < 3:277747/134‑1 (MQ=255) cAAGCCGGCAAA‑GCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTGCACTCATGGGCTTACACCGTGTGCAg > 3:349074/1‑136 (MQ=255) aaGCCGGAAAT‑GCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTGCGCTCATGGGCTTACACCGTGTGCAgg < 8:243736/136‑1 (MQ=255) cGGCAAA‑GCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTGCGCTCATGGGCTTACACCGTGTGCAGGTAc > 5:170795/1‑135 (MQ=255) cGGCAAA‑GCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTGCGCTCATGGGCTTACACCGTGTGCAGGTAc > 7:54164/1‑135 (MQ=255) cGGCAAA‑GCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACATGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTGCGCTCATGGGCTTACACCGTGTGCAGGTAc > 5:351836/1‑135 (MQ=255) | CTGGGGGCGCCATTGTAATGATGCGGCTCGATATTTGAAGGCGAGCCGGTAAAAAC‑AATGCCGTGCAGGCGAGCCAGCAGCGCTTGCGTGTCGCTGCCGCCATCACGGGCCGGC‑AAGATCAGTGGCAAGCCGGCAAA‑GCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTGCGCTCATGGGCTTACACCGTGTGCAGGTAC > ACB143/6043063‑6043327 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |