| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | ACB143 | 2,668,473 | 0 | A | C | 55.6% | 1.1 / 11.1 | 19 | G234G (GGT→GGG) | CJ019_02525 | hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base A (5/3); new base C (9/1); total (15/4) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.75e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.10e-02 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CAGGGCCTTGTCGAACCAGCCGGGTACCACCTTGTCGGTGAAGTGCGGGTACA‑GCACCAGGTCGTCGCCGCGATAGGGCAGGTCGAGGTGATAGTCGAGCAGGCCGCCGTCGCGGTAGGTG‑CCGGCGCCGGCACCGGGGATGTCTTTCACGCCCTGCATGACCATCGGTATCGACCCGGAAGCGAGCAGGGCGTGGCGCAGGTTGGTCAAGTCCAGCGGCAGGTAGCGTGAGGGGAAGTCGGTCAGCGTGTCGAGCGGAGGCGCGGCGC > ACB143/2668341‑2668609 | cagggcCTTGTCGAACCAGCCGGGTACCACCTTGTCGGTGAAGTGCGGGTACA‑GCACCAGGTCGTGGCCGCGATAGGGCAGGTCGAGGTGTTAGTCGAGCAGGCCGCCGTCGCGGTTGGTG‑CCGGCGCCGGGCCCgg > 8:114416/1‑137 (MQ=255)cagggcCTTGTCGAACCAGCCGGGTACCACCTTGTCGGTGAAGTGCGGGTACA‑GCACCAGGTCGTCGCCGCGATAGGGCAGGTCGAGGTGATAGTCGAGCAGGCCGCCGTCGCGGTAGGTGCCCGCCGCCGCCAcgg > 8:48013/1‑135 (MQ=255)cagggcCTTGTCGAACCAGCCGGGTACCACCTTGTCGGTGAAGTGCGGGTACA‑GCACCAGGTCGTCGCCGCGATAGGGCAGGTCGAGGTGATAGTCGAGCAGGCCGCCGTCGCGGTAGGTG‑GCGGCGCCGGCCCCgg > 1:37474/1‑137 (MQ=255)cagggcCTTGTCGAACCAGCCGGGTACCACCTTGTCGGTGAAGTGCGGGTACA‑GCACCAGGTCGTCGCCGCGATAGGGCAGGTCGAGGTGATAGTCGAGCAGGCCGCCGTCGCGGTAGGTG‑CCGGCGCCGGCACCgg > 5:236880/1‑137 (MQ=255) ggggcCTTGTCTACCCCGCCGGGTACCACCTTGGCGGTGAAGTGCGGGTACA‑GCACCAGGTCGTCGCCGCGATAGGGCAGGGCGCGGGGATAGGCGAGCAGGCCGCCGGCGCGTTAGGGG‑CCGGCGCCGGCACCggg < 6:128635/136‑1 (MQ=255) ggcCTTGTCGAACCAGCCGGGTACCACCTTGTCGGTGAAGTGCGGGTACA‑GCACCAGGTCGTCGCCGCGATAGGGCAGGTCGAGGTGATAGTCGAGCAGGCCGCCGTCGCGGTAGGTG‑CCGGCCGCGGCCCCggggg > 5:12974/1‑136 (MQ=255) cTTGTCGAACCAGCCGGGTACCACCTTGTCGGTGAAGTGCGGGTACA‑GCACCAGTTCGTCGACGCGATAGGGCAGGTCGAGGTGATAGTCGAGCAGGCCGCCGTCGCGGTAGGTG‑CCCGCGCCGGCCCCGGGGCTGt > 2:100515/1‑137 (MQ=255) cTTGTCGAACCAGCCGGGTACCACCTTGTCGGTGAAGTGCGGGTACA‑GCACCAGGTCGTCGCCGCGATAGGGCAGGTCGAGGTGATAGTCGAGCAGGCCGCCGTCGCGGTAGGTG‑CCGGCGCCGGCCCCGGGGGTgg > 3:83538/1‑136 (MQ=255) tCGAACCAGCCGGGTACCACCTTGTCGGTGAAGTGCGGGTACA‑GCACCAGGTCGTCGCCGCGATAGGGCAGGTCGAGGTGATAGTCGAGCAGGCCGCCGTCGCGGTAGGTG‑GCCGCGGCGGCGCCGGGGGTgggttt > 5:187394/1‑132 (MQ=255) cGACCCCGCCGGGACCCCCATTGGGGGTGAGGTGGGGGTACA‑CCACCTGGGGGGGCCCGCTTTTGGGCTGGTCCTGGTTTTCGTCGTGCTGCCCGCGGTCGGGGTTGGTG‑CCGGCGCGGGCACCGGGGATGTCTTTc < 4:154620/137‑1 (MQ=255) cGAACCAGCCGGGTACCACCTTGTCGGTGAAGTGCGGGTACA‑GCACCAGGTCGTCGCCGCGATACGGAAGGTCGAGGTGATAGTCGAGAAGGCCGCCGTCGCGGTAGGTG‑CCGGCGCCGGCCCCCGGGATGTGtttg > 6:258931/1‑136 (MQ=255) ttGTCGGTGAAGTGCGGGTACA‑GCACCAGGTCGTCGCCGCGATAGGGCAGGTCGAGGTGATAGTCGAGCAGGCCGCCGTCGCGGTAGGTG‑CCGGCGCCGGCCCCGGGGGTGTCTTTAACCCCCGGCATGACCaaagg > 7:88055/1‑133 (MQ=255) tggTCGGTGACGTG‑GGGTAAACCCACCTGGGCGGCGCCGCGTTCGGGCAGGTCGGGTTTATTGTCGCGCAGGCCGCCGGCGGGGTCGGTG‑CGGGCGCCGCCCCCGGGGATGTCTTTCACGCCCTGCATGACCATCgg < 2:253928/135‑1 (MQ=255) tGTCGGTGAAGTGCGGGTACA‑GCACCAGGTCGTCGCCGCGATAGGGCAGGTCGAGGTGATAGTCGAGCAGGCCGCCGTCGCGGTAGGTG‑CCGGCGCCGGCCCCGGGGATGGCTTTCACGCCCTGCATGCCCATCGGt > 8:239756/1‑137 (MQ=255) ggTGAAGTGCGGGTACA‑GCACCAGGTCGTCGCCGCGATAGGGCAGGTCGAGGTGATAGTCGAGCAGGCCGCCGTCGCGGTAGGTT‑CCGGCGCCCGCACCGGGGTTGTCTTTAACCCCCGTCATGACCATCGGTAtag > 4:16926/1‑135 (MQ=255) cgtcgCCGCGATAGGGCAGGTCGAGGTGATAGTCGAGCAGGCCGCCGTCGCGGTAGGTG‑CCGGCGCCGGCCCCGGGGATGTCTTTAACGCCCTGGATGACCATAGGTATCGACCCCGAAGCGAGCAGGGGGTGgcgg > 3:73813/1‑136 (MQ=255) cgtcgCCGCGATAGGGCAGGTCGAGGTGATAGTCGAGCAGGCCGCCGTCGCGGTAGGTG‑CCGGCGCCGGCACCGGGGATGTCTTTAACGCCCTGCATGACCATCGGTATAGACCCGGAAGCGAGCAGGGCGTGgcgc > 5:131094/1‑137 (MQ=255) ccgATCGGGCCGGGCGCGGTCATAGGCCAGCCGGCCGCGGCCGGGGTAGGGT‑CCGGCGCTGGCACCGGGGATGTCTTTCACGCCCTGCATGACCATCGGTATCGACCCGGAAGCGAGCAGGGCGTGGCGCAGGTTgg < 8:66883/136‑1 (MQ=255) gccggcACCGGGGATGTCTTTCACGCCCTGCATGACCATCGGTATCGACCCGGAAGCGAGCAGGGCGTGGCGCAGGTTGGTCAATTCCAGCGGCAGGTAGAGTGAGGGGAAGTCGGTCAGCGTGTCGAGCGGAggcg > 7:185900/1‑137 (MQ=255) aCCGGGGATGTCTTTCACGCCCTGCATGACCATCGGTATCGACCCGGAAGCGAGCAGGGCGTGGCGCAGGTTGGTCAAGTCCAGCGGCAGGTAGCGTGAGGGGAAGTCGGTCAGCGTGTCGAGCGGAggcgcggcgc > 4:183872/1‑137 (MQ=255) aCCGGGGATGTCTTTCACCCCCTGCATGACCATCGGTATCGACCCGGAAGCGAGCAGGGCGTGGCGCAGGTTGGTCAAGTACAGCGGCAGGTAGCGTGAGGGGAAGTCGGTAAGCGTGTCGAGCGGAggcgcggcgc > 6:38759/1‑137 (MQ=255) | CAGGGCCTTGTCGAACCAGCCGGGTACCACCTTGTCGGTGAAGTGCGGGTACA‑GCACCAGGTCGTCGCCGCGATAGGGCAGGTCGAGGTGATAGTCGAGCAGGCCGCCGTCGCGGTAGGTG‑CCGGCGCCGGCACCGGGGATGTCTTTCACGCCCTGCATGACCATCGGTATCGACCCGGAAGCGAGCAGGGCGTGGCGCAGGTTGGTCAAGTCCAGCGGCAGGTAGCGTGAGGGGAAGTCGGTCAGCGTGTCGAGCGGAGGCGCGGCGC > ACB143/2668341‑2668609 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |