Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	ACB143	5,569,213	A→C	5.5%	C36G (TGC→GGC)	CJ019_05240 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	ACB143	5,569,213	0	A	C	5.5%	228.1 / 4.5	72	C36G (TGC→GGC)	CJ019_05240	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (33/34);  new base C (2/2);  total (36/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.85e-01

TTGGCGCAATGAAAATGGTCAGCAGCGCCGCAAAACTGGTCAGCACCAGGTACTCAGGGTGCTTCACCCAGGTCAGCGGGTAGTGCCCGAGCAGCACATCACGCACCGAGCCACCCCCAAGCGCAGTGACGCAGGCGATCAGCACCACGCCAAACCAGTCCATGCCGCGACGCCCGGCAGACAGCGCGCCAGTCATGGCTTCGGCGGTAATGGCGATAAGGTAGAGCATCAACAACATGGTGCGGGTCCGTGCAGGAAAGGCGCG  >  ACB143/5569081‑5569345                                                                                                                                     |                                                                                                                                     ttGGCGCAATGAAAATGGTCAGCAGCGCCGCAAAACTGGTCAGCACCAGGTACTCAGGGTGCTTCACCCAGGTCAGCGGGTAGTGCCCGAGCAGCACATCACGCACCGAGCCACCCCCAAGCGCAGTGACGCAGGc                                                                                                                                   <  1:327573/136‑1 (MQ=255) ttGGCGCAATGAAAATGGTCAGCAGCGCCGCAAAACTGGTCAGCACCAGGTACTCAGGGTGCTTCACCCAGGTCAGCGGGTAGTGCCCGAGCAGCACATCACGCACCGAGCCACCCCCAAGCGCAGTGACGCAGGc                                                                                                                                   <  2:176763/136‑1 (MQ=255)       cAATGAAAATGGTCAGCAGCGCCGCAAAACTGGTCAGCACCAGGTACTCAGGGTGCTTCACCCAGGTCAGCGGGTAGTGCCCGAGCAGCACATCACGCACCGAGCCACCCCCAAGCGCAGTGACGCAGGCGATCAg                                                                                                                             <  3:1763/136‑1 (MQ=255)       cAATGAAAATGGTCAGCAGCGCCGCAAAACTGGTCAGCACCAGGTACTCAGGGTGCTTCACCCAGGTCAGCGGGTAGTGCCCGAGCAGCACATCACGCACCGAGCCACCCCCAAGCGCAGTGACGCAGGCGATCAg                                                                                                                             <  2:74473/136‑1 (MQ=255)                tGGTCAGCAGCGCCGCAAAACTGGTCAGCACCAGGTACTCAGGGTGCTTCACCCAGGTCAGCGGGTAGTGCCCGAGCAGCACATCACGCACCGAGCCACCCCCAAGCGCAGTGACGCAGGCGATCAGCACCACGcc                                                                                                                    <  6:226104/136‑1 (MQ=255)                             cgcAAAACTGGTCAGCACCAGGTACTCAGGGTGCTTCACCCAGGTCAGCGGTTAGTGCCCGAGCAGCACATCACGCACCGAGCCACCCCCAAGCGCAGTGACGCAGGCGATCAGCACCACGCCAAACCAGTCCATg                                                                                                       <  8:156840/136‑1 (MQ=255)                               cAAAACTGGTCAGCACCAGGTACTCAGGGTGCTTCACCCAGGTCAGCGGGTAGTGCCCGAGCAGCACATCACGCACCGAGCCACCCCCAAGCGCAGTGACGCAGGCGATCAGCACCACGCCAAACCAGTCCATGcc                                                                                                     <  2:56775/136‑1 (MQ=255)                                  aaCTGGTCAGCACCAGGTACTCAGGGTGCTTCACACAGGTCAGCTGGTAGTGCCCGAGCAGCACATCACGCACCGAGCCACCCCCAAGCGCAGTGACGCGGGCGATCAGCACCACGCCAAACCAGTCCATGCcgc                                                                                                   >  3:29738/1‑135 (MQ=255)                                          aGCACCAGGTACTCAGGGTGCTTCACCCAGGTCAGCGGGTAGTGCCCGAGCAGCACATCACGCACCGAGCCACCCCCAAGCGCAGTGACGCAGGCGATCAGCACCACGCCAAACCAGTCCATGCCGCGACGCCCgg                                                                                          >  4:2289/1‑136 (MQ=255)                                          aGCACCAGGTACTCAGGGTGCTTCACCCAGGTCAGCGGGTAGTGCCCGAGCAGCACATCACGCACCGAGCCACCCCCAAGCGCAGTGACGCAGGCGATCAGCACCACGCCAAACCAGTCCATGCCGCGACGCCCgg                                                                                          >  2:61647/1‑136 (MQ=255)                                          aGCACCAGGTACTCAGGGTGCTTCACCCAGGTCAGCGGGTAGTGCCCGAGCAGCACATCACGCACCGAGCCACCCCCAAGCGCAGTGACGCAGGCGATCAGCACCACGCCAAACCAGTCCATGCCGCGACGCCCgg                                                                                          >  3:55701/1‑136 (MQ=255)                                                  gTACTCAGGGTGCTTCACCCAGGTCAGCGGGTAGTGCCCGAGCAGCACATCACGCACCGAGCCACCCCCAAGCGCAGTGACGCAGGCGATCAGCACCACGCCAAACCAGTCCATGCCGCGACGCCCGGCAGACAgc                                                                                  <  5:165191/136‑1 (MQ=255)                                                    aCTCAGGGTGCTTCACCCAGGTCAGCGGGTAGTGCCCGAGCAGCACATCACGCACCGAGCCACCCCCAAGCGCAGTGACGCAGGCGATCAGCACCACGCCAAACCAGTCCATGCCGCGACGCCCGGCAGACAgcgc                                                                                >  1:84894/1‑136 (MQ=255)                                                    aCTCAGGGTGCTTCACCCAGGTCAGCGGGTAGTGCCCGAGCAGCACATCACGCACCGAGCCACCCCCAAGCGCAGTGACGCAGGCGATCAGCACCACGCCAAACCAGTCCATGCCGCGACGCCCGGCAGACAgcgc                                                                                >  4:248735/1‑136 (MQ=255)                                                         gggTTCTTCACCAAGGTCATCGGGTAGTGCCCGAGCAGCACATCACGCACCGAGCAACACCCAAGCGCAGTGACGCAGGCGATCAGCACCACGCCAAACCAGTCCATGACGCGACTCCCGGCAGACAGCGCGCCAg                                                                           >  7:293427/1‑136 (MQ=255)                                                         gggTGCTTCACCCAGGTCAGCGGGTAGTGCCCGAGCAGCACATCACGCACCGCGCCACCCCCAAGCGCAGTGACGCAGGCGATCAGCACCCCGCCAACCCAGTCCATGCCGCGACGCCCGGCAGACAGCGCGCCAg                                                                           >  2:158681/1‑136 (MQ=255)                                                         gggTGCTTCACCCAGGTCAGCGGGTAGTGCCCGAGCAGCACATCACGCACCGAGCCACCCCCAAGCGCAGTGACGCAGGCGATCAGCACCACGCCAAACCAGTCCATGCCGCGACGCCCGGCAGACAGCGCGCCAg                                                                           >  5:384678/1‑136 (MQ=255)                                                         gggTGCTTCACCCAGGTCAGCGGGTAGTGCCCGAGCAGCACATCACGCACCGAGCCACCCCCAAGCGCAGTGACGCAGGCGATCAGCACCACGCCAAACCAGTCCATGCCGCGACGCCCGGCAGACAGCGCGCCAg                                                                           >  4:384828/1‑136 (MQ=255)                                                         gggTGCTTCACCCAGGTCAGCGGGTAGTGCCCGAGCAGCACATCACGCACCGAGCCACCCCCAAGCGCAGTGACGCAGGCGATCAGCACCACGCCAAACCAGTCCATGCCGCGACGCCCGGCAGACAGCGCGCCAg                                                                           >  7:242092/1‑136 (MQ=255)                                                         gggTGCTTCACCCAGGTCAGCGGGTAGTGCCCGAGCAGCACATCACGCACCGAGCCACCCCCAAGCGCAGTGACGCAGGCGATCAGCACCACGCCAAACCAGTCCATGCCGCGACGCCCGGCAGACAGCGCGCCAg                                                                           >  2:92285/1‑136 (MQ=255)                                                         gggTGCTTCACCCAGGTCAGCGGGTAGTGCCCGAGCAGCACATCACGCACCGAGCCACCCCCAAGCGCAGTGACGCAGGCGATCAGCACCACGCCAAACCAGTCCATGCCGCGACGCCCGGCAGACAGCGCGCCAg                                                                           >  1:225826/1‑136 (MQ=255)                                                               ttCACCCAGGTCAGCGGGTAGTGCCCGAGCAGCACATCACGCACCGAGCCACCCCCAAGCGCAGTGACGCAGGCGATCAGCACCACGCCAAACCAGTCCATGCCGCGACGCCCGGCAGACAGCGCGCCAGTCATgg                                                                     >  4:94790/1‑136 (MQ=255)                                                                tCACCCAGGTCAGCGGGTAGTGCCCGAGCAGCACATCACGCACCGAGCCACCCCCAAGCGCAGTGATGCAGGCGATCAGCACCACGCCAAACCAGTCCATGCCGCGACGCCCGGCAGACAGCGCGCCAGTCATGGc                                                                    >  5:266139/1‑136 (MQ=255)                                                                tCACCCAGGTCAGCGGGTAGTGCCCGAGCAGCACATCACGCACCGAGCCACCCCCAAGCGCAGTGACGCAGGCGATCAGCACCACGCCAAACCAGTCCATGCCGCGACGCCCGGCAGACAGCGCGCCAGTCATGGc                                                                    >  5:360826/1‑136 (MQ=255)                                                                         tCAGCGGGTAGTGCCCGATCAGCACATCACGCACCGAGCCACCCCCAAGCGCAGTGACGCAGGCGATCAGCACCACGCCAAACCAGTCCATGCCGCGACGCCCGGCAGACAGCGCGCCAGTCATGGCTTCGGCGGt                                                           <  6:18999/136‑1 (MQ=255)                                                                         gccgCGGGTAGGGCCCGAGCAGCACATCACGAACCGCGCCCCCCCCAAGCGCAGTGACGCAGGCGAGCAGCACCCCGCCAAACCAGTCCATGCCGCGACGCCCGGCAGACAGCGCGCCAGTCATGGCTTCGGCGGt                                                           <  2:363838/133‑1 (MQ=255)                                                                           aGCGGGTAGTGCCCGAGCAGCACATCACGCACCGAGCCACCCCCAAGCGCAGTGACGCAGGCGATCAGCACCACGCCAAACCAGTCCATGCCGCGACGCCCGGCAGACAGCGCGCCAGTCATGGCTTCGGCGGTaa                                                         >  7:90927/1‑136 (MQ=255)                                                                              gggTAGTGCCCGAGCAGCACATCACGCACCGAGCCACCCCCAAGCGCAGTGACGCAGGCGATCAGCACCACGCCAAACCAGTCCATGCCGCGACGCCCGGCAGACAGCGCGCCAGTCATGGCTTCGGCGGTAATgg                                                      <  1:40383/136‑1 (MQ=255)                                                                              gggTAGTGCCCGAGCAGCACATCACGCACCGAGCCACCCCCAAGCGCAGTGACGCAGGCGATCAGCACCACGCCAAACCAGTCCATGCCGCGACGCCCGGCAGACAGCGCGCCAGTCATGGCTTCGGCGGTAATgg                                                      <  2:312155/136‑1 (MQ=255)                                                                                gTAGTGCCCGAGCAGCACATCACGCACCGAGCCACCCCCAAGCGCAGTGACGCAGGCGATCAGCACCACGCCAAACCAGTCCATGCCGCGACGCCCGGCAGACAGCGCGCCAGTCATGGCTTCGGCGGTAATGGc                                                     >  5:346924/1‑135 (MQ=255)                                                                                        cGAGCAGCACATCACGCACCGAGCCACCCCCAAGCGCAGTGACGCAGGCGATCAGCACCACGCCAAACCAGTCCATGCCGCGACGCCCGGCAGACAGCGCGCCAGTCATGGCTTCGGCGGTAATGGCGATAAgg                                              >  5:374025/1‑134 (MQ=255)                                                                                         gAGCAGCACATCACGCACCGAGCCACCCCCAAGCGCAGTGACGCAGGCGATCAGCACCACGCCAAACCAGTCCATGCCGCGACGCCCGGCAGACAGCGCGCCAGTCATGGCTTCGGCGGTAATGGCGATAAGGTag                                           <  8:242092/136‑1 (MQ=255)                                                                                         gAGCAGCACATCACGCACCGAGCCACCCCCAAGCGCAGTGACGCAGGCGATCAGCACCACGCCAAACCAGTCCATGCCGCGACGCCCGGCAGACAGCGCGCCAGTCATGGCTTCGGCGGTAATGGCGATAAGGTag                                           <  2:135139/136‑1 (MQ=255)                                                                                           gcagcaCATCACGCACCGAGCCACCCCCAAGCGCAGTGACGCAGGCGATCAGCACCACGCCAAACCAGTCCATGCCGCGACGCCCGGCAGACAGCGCGCCAGTCATGGCTTCGGCGGTAATGGCGATAAGGTagag                                         <  7:128377/136‑1 (MQ=255)                                                                                             agcaCATCACGCACCGAGCCACCCCCAAGCGCAGTGACGCCGGCGATCAGCACCACGCCAAACCAGTCCATGCCGCGACGCCCGGCAGACAgcg                                                                                 >  6:49855/1‑94 (MQ=255)                                                                                             agcaCATCACGCACCGAGCCACCCCCAAGCGCAGTGACGCCGGCGATCAGCACCACGCCAAACCAGTCCATGCCGCGACGCCCGGCAGACAgcg                                                                                 >  6:45211/1‑94 (MQ=255)                                                                                             agcaCATCACGCACCGAGCCACCCCCAAGCGCAGTGACGCCGGCGATCAGCACCACGCCAAACCAGTCCATGCCGCGACGCCCGGCAGACAgcg                                                                                 <  5:49855/94‑1 (MQ=255)                                                                                             agcaCATCACGCACCGAGCCACCCCCAAGCGCAGTGACGCCGGCGATCAGCACCACGCCAAACCAGTCCATGCCGCGACGCCCGGCAGACAgcg                                                                                 <  5:45211/94‑1 (MQ=255)                                                                                             agcaCATCACGCACCGAGCCACCCCCAAGCGCAGTGACGCAGGCGATCAGCACCACGCCAAACCAGTCCATGCCGCGACGCCCGGCAGACAGCGCGCCAGTCATGGCTTCGGCGGTAATGGCGATAAGGTAGAGCa                                       >  1:237446/1‑136 (MQ=255)                                                                                             agcaCATCACGCACCGAGCCACCCCCAAGCGCAGTGACGCAGGCGATCAGCACAACGCCAAACCAGTCCATGCCGCGACGCCCGGCAGACAGCGCGCCAGTCAGGGCTTCGGCGGTAATGGCGATAAGGTAGAGCa                                       >  6:337494/1‑136 (MQ=255)                                                                                                  aTCACGCACCGAGCCACCCCCAAGCGCAGTGACGCAGGCGATCAGCACCACGCCAAACCAGTCCATGCCGCGACGCCCGGCAGACAGCGCGCCAGTCATGGCTTCGGCGGTAATGGCGATAAGGTAGAGCATcaa                                   >  4:86846/1‑135 (MQ=255)                                                                                                   tCACGCACCGAGCCACCCCCAAGCGCAGTGACGCAGGCGATCAGCACCACGCCAAACCAGTCCATGCCGCGACGCCCGGCAGACAGCGCGCCAGTCATGGCTTCGGCGGTAATGGCGATAAGGTAGAGCATcaaca                                 <  5:378358/136‑1 (MQ=255)                                                                                                   tCACGCACCGAGCCACCCCCAAGCGCAGTGACGCAGGCGATCAGCACCACGCCAAACCAGTCCATGCCGCGACGCCCGGCAGACAGCGCGCCAGTCATGGCTTCGGCGGTAATGGCGATAAGGTAGAGCATcaaca                                 <  6:360826/136‑1 (MQ=255)                                                                                                   tCACGCACCGAGCCACCCCCAAGCGCAGTGACGCAGGCGATCAGCACCACGCCAAACCAGTCCATGCCGCGACGCCCGGCAGACAGCGCGCCAGTCATGGCTTCGGCGGTAATGGCGATAAGGTAGAGCATcaaca                                 <  5:392305/136‑1 (MQ=255)                                                                                                   tCACGCACCGAGCCACCCCCAAGCGCAGTGACGCAGGCGATCAGCACCACGCCAAACCAGTCCATGCCGCGACGCCCGGCAGACAGCGCGCCAGTCATGGCTTCGGCGGTAATGGCGATAAGGTAGAGCATcaaca                                 <  1:317875/136‑1 (MQ=255)                                                                                                   tCACGCACCGAGCCACCCCCAAGCGCAGTGACGCAGGCGATCAGCACCACGCCAAACCAGTCCATGCCGCGACGCCCGGCAGACAGCGCGCCAGTCATGGCTTCGGCGGTAATGGCGATAAGGTAGAGCATcaaca                                 <  1:292553/136‑1 (MQ=255)                                                                                                   tCACGCACCGAGCCACCCCCAAGCGCAGTGACGCAGGCGATCAGCACCACGCCAAACCAGTCCATGCCGCGACGCCCGGCAGACAGCGCGCCAGTCATGGCTTCGGCGGTAATGGCGATAAGGTAGAGCATcaaca                                 <  5:188695/136‑1 (MQ=255)                                                                                                             aGCCACCCCCAAGCGCAGTGACGCAGGCGATCAGCACCACGCCAAACCAGTCCCTGCCGCGACGCCCGGCAGACAGCGCGCCAGTCATGGCTTCGGCGGTAATGGCGATAAGGTAGAGCATCAACAACATGGTGCg                       <  4:268281/136‑1 (MQ=255)                                                                                                             aGCCACCCCCAAGCGCAGTGACGCAGGCGATCAGCACCACGCCAAACCAGTCCATGCCGCGACGCCCGGCAGACAGCGCGCCAGTCATGGCTTCGGCGGTAATGGCGATAAGGTAGAGCATCAACAACATGGTGCg                       <  3:39095/136‑1 (MQ=255)                                                                                                             aGCCACCCCCAAGCGCAGTGACGCAGGCGATCAGCACCACGCCAAACCAGTCCATGCCGCGACGCCCGGCAGACAGCGCGCCAGTCATGGCTTCGGCGGTAATGGCGATAAGGTAGAGCATCAACAACATGGTGCg                       <  3:33768/136‑1 (MQ=255)                                                                                                               ccACCCCCAAGCGCAGTGACGCAGGCGTTCAGCACCACGCCAAACCAGTCCATGCCGCGACGCCCGGCAGACAGCGCGCCAGTCATGGCTTCGGCGGTAATGGCGATAAGGTAGAGCATCAACAACATGGTGCggg                     >  5:4822/1‑136 (MQ=255)                                                                                                               ccACCCCCAAGCGCAGTGACGCAGGCGATCAGCACCACGCCAAACCAGTCCATGCCGCGACGCCCGGCAGACAGCGCGCCAGTCATGGCTTCGGCGGTAATGGCGATAAGGTAGAGCATCAACAACATGGTGCggg                     >  6:58332/1‑136 (MQ=255)                                                                                                                cACCCCCAAGCGCAGTGACGCAGGCGATCAGCACCACGCCAAACCAGTCCATGCCGCGACGCCCGGCAGACAGCGCGCCAGTCATGGCTTCGGCGGTAATGGCGATAAGGTAGAGCATCAACAACATGGTGCGGGt                    >  4:57429/1‑136 (MQ=255)                                                                                                                      cAAGCGCAGTGACGCAGGCGATCAGCACCACGCCAAACCAGTCCATGCCGCGACGCCCGGCAGACAGCGCGCCAGTCATGGCTTCGGCGGTAATGGCGATAAGGTAGAGCATCAACAACATGGTGCGGGTCCGTg               >  5:277348/1‑135 (MQ=255)                                                                                                                           gcAGTGACGCAGGCGATCAGCACCACGCCAAACCAGTCCATGCCGCGACGCCCGGCAGACAGCGCGCCAGTCATGGCTTCGGCGGTAATGGCGATAAGGTAGAGCATCAACAACATGGTGCGGGTCCGTGCAGGa          >  4:284423/1‑135 (MQ=255)                                                                                                                           gcAGTGACGCAGGCGATCAGCACCACGCCAAACCAGTCCATGCCGCGACGCCCGGCAGACAGCGCGCCAGTCATGGCTTCGGCGGTAATGGCGATAAGGTAGAGCATCAACAACATGGTGCGGGTCCGTGCAGGa          >  1:222334/1‑135 (MQ=255)                                                                                                                              gTGACGCAGGCGATCAGCACCACGCCAAACCAGTCCATGCCGCGACGCCCGGCAGACAGCGCGCCAGTCATGGCTTCGGCGGTAATGGCGATAAGGTAGAGCATCAACAACATGGTGCGGGTCCGTGCAGGAAAgg      <  6:60173/136‑1 (MQ=255)                                                                                                                              gTGACGCAGGCGATCAGCACCACGCCAAACCAGTCCATGCCGCGACGCCCGGCAGACAGCGCGCCAGTCATGGCTTCGGCGGTAATGGCGATAAGGTAGAGCATCAACAACATGGTGCGGGTCCGTGCAGGAAAgg      <  3:248735/136‑1 (MQ=255)                                                                                                                               tGATGCAGGCGATCAGCACCACGCCAAACCAGTCCATGCCGCGACGCCCGGCAGACAGCGCGCCAGTCATGGCTTCGGCGGTAATGGCGATAAGGTAGAGCATCAACAACATGGTGCGGGTCCGTGCAGGAAAGgc     <  6:266139/136‑1 (MQ=255)                                                                                                                               tGACGCAGGCGATCAGCACCACGCCAAACCAGTCCATGCCGCGACGCCCGGCAGACAGCGCGCCAGTCATGGCTTCGGCGGTAATGGCGATAAGGTAGAGCATcaaca                                 <  3:264729/108‑1 (MQ=255)                                                                                                                               tGACGCAGGCGATCAGCACCACGCCAAACCAGTCCATGCCGCGACGCCCGGCAGACAGCGCGCCAGTCATGGCTTCGGCGGTAATGGCGATAAGGTAGAGCATCAACAACATGGTGCGGGTCCGTGCAGGAAAGgc     <  6:57749/136‑1 (MQ=255)                                                                                                                               tGACGCAGGCGATCAGCACCACGCCAAACCAGTCCATGCCGCGACGCCCGGCAGACAGCGCGCCAGTCATGGCTTCGGCGGTAATGGCGATAAGGTAGAGCATCAACAACATGGTGCGGGTCCGTGCAGGAAAGgc     <  6:277348/136‑1 (MQ=255)                                                                                                                               tGACGCAGGCGATCAGCACCACGCCAAACCAGTCCATGCCGCGACGCCCGGCAGACAGCGCGCCAGTCATGGCTTCGGCGGTAATGGCGATAAGGTAGAGCATCAACAACATGGTGCGGGTCCGTGCAGGAAAGgc     <  5:265113/136‑1 (MQ=255)                                                                                                                               tGACGCAGGCGATCAGCACCACGCCAAACCAGTCCATGCCGCGACGCCCGGCAGACAGCGCGCCAGTCATGGCTTCGGCGGTAATGGCGATAAGGTAGAGCATCAACAACATGGTGCGGGTCCGTGCAGGAAAGgc     <  4:75993/136‑1 (MQ=255)                                                                                                                               tGACGCAGGCGATCAGCACCACGCCAAACCAGTCCATGCCGCGACGCCCGGCAGACAGCGCGCCAGTCATGGCTTCGGCGGTAATGGAGATAAGGTAGAGCATcaaca                                 >  4:264729/1‑108 (MQ=255)                                                                                                                               tGACGCAGGCGATCAGCACCACGCCAAACCAGACCCTGCCGCGACGCCCGGCAGACAGCGCGCCAGTCATGGCTTCGGCGGTAATGGCGATAAGGTAGAGCATCAACAACATGGTGCGGGTCCGTGCAGGAAAGgc     <  8:221795/136‑1 (MQ=255)                                                                                                                                gACGCAGGCGATCAGCACCACGCCAAACCAGTCCATGCCGCGACGCCCGGCAGACAGCGCGCCAGTCATGGCTTCGGCGGTAATGGCGATAAGGTAGAGCATCAACAACATGGTGCGGGTCCGTGCAGGAAAGgcg    >  2:89715/1‑136 (MQ=255)                                                                                                                                gACGCAGGCGATCAGCACCACGCCAAACCAGTCCATGCCGCGACGCCCGGCAGACAGCGCGCCAGTCATGGCTTCGGCGGTAATGGCGATAAGGTAGAGCATCAACAACATGGTGCGGGTCCGTGCAGGAAAGgcg    >  7:167629/1‑136 (MQ=255)                                                                                                                                gACGCAGGCGATCAGCACCACGCCAAACCAGTCCATGCCGCGACGCCCGGCAGACAGCGCGCCAGTCATGGCTTCGGCGGTAATGGCGATAAGGTAGAGCATCAACAACATGGTGCGGGTCCGTGCAGGAAAGgcg    >  5:266189/1‑136 (MQ=255)                                                                                                                                 aCGCAGGCGATCAGCACCACGCCAAACCAGTCCATGCCGCGACGCCCGGCAGACAGCGCGCCAGTCATGGCTTCGGCGGTAATGGCGATAAGGTAGAGCATCAACAACATGGTGCGGGTCCGTGCAGGAAAGgcg    <  2:311754/135‑1 (MQ=255)                                                                                                                                  cGCAGGCGATCAGCACCACGCCAAACCAGTCCATGCCGCGACGCCCGGCAGACAGCGCGCCAGTCATGGCTTCGGCGGTAATGGCGATAAGGTAGAGCATCAACAACATGGTGCGGGTCCGTGCAGGAAAGgcgcg  >  1:365267/1‑136 (MQ=255)                                                                                                                                  cGCAGGCGATCAGCACCACGCCAAACCAGTCCATGCCGCGACGCCCGGCAGACAGCGCGCCAGTCATGGCTTCGGCGGTAATGGCGATAAGGTAGAGCATCAACAACATGGTGCGGGTCCGTGCAGGAAAGgcgcg  >  7:353664/1‑136 (MQ=255)                                                                                                                                     |                                                                                                                                     TTGGCGCAATGAAAATGGTCAGCAGCGCCGCAAAACTGGTCAGCACCAGGTACTCAGGGTGCTTCACCCAGGTCAGCGGGTAGTGCCCGAGCAGCACATCACGCACCGAGCCACCCCCAAGCGCAGTGACGCAGGCGATCAGCACCACGCCAAACCAGTCCATGCCGCGACGCCCGGCAGACAGCGCGCCAGTCATGGCTTCGGCGGTAATGGCGATAAGGTAGAGCATCAACAACATGGTGCGGGTCCGTGCAGGAAAGGCGCG  >  ACB143/5569081‑5569345

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑