| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | ACB143 | 759,412 | C→G | 31.5% | intergenic (+21/‑147) | mmuM_1 → / → CJ019_00729 | Homocysteine S‑methyltransferase/L‑methionine gamma‑lyase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | ACB143 | 759,412 | 0 | C | G | 31.5% | 32.9 / 5.5 | 37 | intergenic (+21/‑147) | mmuM_1/CJ019_00729 | Homocysteine S‑methyltransferase/L‑methionine gamma‑lyase |
| Reads supporting (aligned to +/- strand): ref base C (13/12); new base G (8/4); total (21/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.91e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GGGTTACCTGGCATGGGCCAAGGACTGGCGTGCGCGGGGTGCCAGCATGGTCGGCGGTTGCTGTGGCATCGGCCCGGAGCACATCGCCGAGCTGAAACGCAACCTGGCCTAAGCGCCCACGTGGCCGGGCGGC‑TTTTGCAGCGCCCGGCCCGCAGCCATTAAAAAAAACGCCAGTCAGACCCGGATTCCATCGTTTTTCAGGCCTCCGTCATCTCCCTATAGTCCGCTGAAGGCCGATGCTGCATCGGTGTACACAACTC > ACB143/759280‑759539 | gggTTACCTGGCATGGGCCAAGGACTGGCGTGCGCGGGGTGCCAGCATGGTCGGCGGTTGCTGTGGCATCGGCCCGGAGCACATCGCCGAGCTGAAACGCAACCTGGCCTAAGCGCCCACGTGGCCGGGCGGC‑ttt > 1:82882/1‑136 (MQ=255) ggTTACCTGGCATGGGCCAAGGACTGGCGTGCGCGGGGTGCCAGCATGGTCGGCGGTTGCTGTGGCATCGGCCCGGAGCACATCGCCGAGCTGAAACGCAACCTGGCCTAAGCGCCCACGTGGCCGGGCGGC‑tttt < 5:15892/136‑1 (MQ=255) cgtGGCATGGGCCAAGGACTGGCGTGCGCGGGGTGCCAGCATGGTCGGCGGTTGCTGTGGCATCGGCCCGGAGCACATCGCCGAGCTGAAACGCAACCTGGCCTAAGCGCCCACGTGGCCGGGCGGC‑TTTTGCAgc < 1:306478/134‑1 (MQ=255) tGGCATGGGCCAAGGACTGGCGTGCGCGGGGTGCCAGCATGGTCGGCGGTTGCTGTGGCATCGGCCCGGAGCACATCGCCGAGCTGAAACGCAACCTGGCCTAAGCGCCCACGTGGCCGGGGGGG‑GTTTTGAgcgc > 6:253665/1‑136 (MQ=255) gggCCAAGGACTGGCGTGCGCGGGGTGCCAGCATGGTCGGCGGTTGCTGTGGCATCGGCCCGGAGCACATCGCCGAGCTGAAACGCAACCTGGCCTAAGCGCCCACGTGGCCGGGCGGG‑GTTTGCAGCgcccggcc > 6:91025/1‑136 (MQ=255) ggCCAAGGACTGGGGTGCGCGGGGTGCCAGCATGGGCGGCGGTTGCTGTGGCAGCGGCCCGGAGCACATCGCCGAGCTGAAACGCAACCTGGCCTAAGCGCCCACGTGGCCGGGCGGC‑TTTTGCAGCgcccggccc < 5:281574/136‑1 (MQ=255) aaGGACTGGCGTGCGCGGGGTGCCAGCATGGTCGGCGGTTGCTGTGGCATCGGCCCGGAGCACATCGCCGAGCTGAAACGCAACCTGGCCTAAGCGCCCACGTGGCCGGGCGGC‑TTTTGCAGCGCCCGGCCCGCAg > 4:293975/1‑136 (MQ=255) cTGGCGTGCGCGGGGTGCCAGCATGGTCGGCGGTTGCTGTGGCATCGGCCCGGAGCACATCGCCGAGCTGAAACGCAACCTGGCCTAAGCGCCCACGTGGCCGGGGGGCGTTTTGC‑GCGCCCCGCCCCCCGCCaat > 4:331330/1‑134 (MQ=255) cTGGCGTGCGCGGGGTGCCAGCATGGTCGGCGGTTGCTGTGGCATCGGCCCGGAGCACATCGCCGAGCTGAAACGCAACCTGGCCTAAGCGCCCACGTGGCCGGGCGGC‑GTTTGCCGCGCCCCGCCCGCCGCCAt > 6:299138/1‑135 (MQ=255) cGTGCGCGGGGTGCCAGCATGGTCGGCGGTTGCTGTGGCATCGGCCCGGAGCACATCGCCGAGCTGAAACGCAACCTGGCCTAAGCGCCCACGTGGCCGGGCGGC‑TTTTGCAGCGCCCCGCCCGCAGCCATTaaa > 3:115350/1‑135 (MQ=255) gTGCGCGGGGTGCCAGCATGGTCGGCGGTTGCTGTGGCATCGGCCCGGAGCACATCGCCGAGCTGAAACGCAACCTGGCCTAAGCGCCCACGTGGCCGGGCGGC‑TTTTGCAGCGCCCGGCCCGCCGCCATTaaaa > 5:97544/1‑135 (MQ=255) gTGCGCGGGGTGCCAGCATGGTCGGCGGTTGCTGTGGCATCGGCCCGGAGCACATCGCCGAGCTGAAACGCAACCTGGCCTAAGCGCCCACGTGGCCGGGCGGC‑TTTTGCAGCGCCCGGCCCCCCGCCAATaaaa > 8:203975/1‑135 (MQ=255) gcgGGGTGCCAGCATGGTCGGCGGTTGCTGTGGCATCGGCCCGGAGCACATCGCCGAGCTGAAACGCAACCTGGCCTAAGCGCCCACGTGGCCGGGCGGG‑ATTTTCAGCGCCCGGCCCGCCGCCAATAAAAAAAAc > 1:129172/1‑136 (MQ=255) ggggTGCCAGCATGGTCGGCGGTTGCTGTGGCATCGGCCCGGAGCACATCGCCGAGCTGAAACGCAACCTGGCCTAAGCGCCCACGTGGCCGGGCGGC‑GTTTGCAGCGCCCGGCCCGCCGCCATTAAAAAAAACGc > 3:332317/1‑136 (MQ=255) ccAGCATGGGCGGCGGTTGCTGTGGCATCGGCCCGGAGCACATCGCCGAGCTGAAACGCAACCTGGCCTAAGCGCCCACGTGGCCGGGCGGC‑TTTTGCAGCGCCCGGCCCGCAGCCATTAAAAAAAACGCCAGTCa < 2:212063/136‑1 (MQ=255) aTGGTCGGCGGTTGCTGTGGCATCGGCCCGGAGCACATCGCCGAGCTGAAACGCAACCTGGCCTAAGCGCCCACGTGGCCGGGCGGG‑GTTTGGAGCGCCCGGCCCCCCGCCCGTTAAAAAAACGCCCGGCAGcccc > 3:258039/1‑136 (MQ=255) gggggTTGCTGTGCCTTGGGCCCGGCCCACAGCGCCCAGCTCAACCCCACCCGGCCCAACGCCCCCAGGGGGCGGGGGGGG‑TTTTCCAGCGCCGGGCCCGCAGCCATTAAAAAAAACGCCAGTCAGACCCGGATTc < 4:368881/133‑1 (MQ=255) ggcggTTGCTGTGGCATCGGCCCGGAGCACATCGCCGAGCTGAAACGCAACCTGGCCTAAGCGCCCACGTGGCCGGGCGGC‑GTTTGCAGCGCCCGGCCCGCCGCCATTAAAAAAAACGCCCGTCAGGCCCGGAttg > 6:187373/1‑135 (MQ=255) tGCTGTGGCATCGGCCCGGAGCACATCGCCGAGCTGAAACGCAACCTGGCCTAAGCGCCCACGTGGCCGGGCGGG‑GGTTGGCGCGCCCCGGCCGCCCCCAATAAAAAAAACCCCACGCAGACCCCGGTTGCAtgcg > 6:241094/1‑133 (MQ=255) cccgtcgCACATCGCCGAGCTTAAACGCACCCTGGCCTAAGCCCCCACGGGGCGGGGCGGC‑TTTTGCAGGGCCCGGCCCGCAGCCATTAAAAAAAACGCCAGTCAGACCCGGATTCCATCGTTTTTCAGGCCTCCg < 5:367997/130‑1 (MQ=255) cccGGAGCACAGCGCCGAGCTGAAACGCTACCTGCCCAACGCGCCCACGGGGCCGGGGGGC‑TTTTGCCGCGCCGGGCCCGCAGCCATTAAAAAAAACGCCAGTCAGACCCGGATTCCATCGTTTTTCAGGCCTCCg < 8:156151/136‑1 (MQ=255) cacGGAGAACAGCGCCTCGCTGAACCCCAACCTGCCCAACGCGCCCCCGGGGGCGGGCGGG‑TTTTGCCGCGCCCGGCCCGCAGCCATTAAAAAAAACGCCAGTCAGACCCGGATTCCATCGTTTTTCAGGCCTCCg < 4:250483/134‑1 (MQ=255) cGGAGCACATCGCCGAGCTGAAACGCAACCTGGCCTAAGCGCCCACGTGGCCGGGCGGC‑TTTTGCAGCGCCCGGCCCGCAGCCATTAAAAAAAACGCCAGTCAGACCCGGATTCCATCGTTTTTCAGGCCTCCGTc < 8:308014/136‑1 (MQ=255) cGGAGCACATCGCCGAGCTGAAACGCAACCTGGCCTAAGCGCCCACGTGGCCGGGCGGC‑GTTTGCCGCGCCCGGCCCGCCGCCACTAAAAAAAACGCCCGTCCGCCCCGGGTTCCAGCGGTTTTGAGGGCGCCggc > 5:293459/1‑134 (MQ=255) cgcgtaGCTTAACCGCACCGTGCCCTACGCCCCCAGGTGGCGGGGGGGC‑TTTTGCCGCGCCGGGCCCGCAGCCATTAAAAAAAACGCCAGTCAGACCCGGATTCCATCGTTTTTCAGGCCTCCGTCATCTCCCtat < 6:78675/131‑1 (MQ=255) cGCCGAGCTGAAACGCTACCTGGCCTAAGCGCCCACGTGGCCGGGCGGG‑GTTTTCAGCGCCCCGCCCGCCGCCAATTAAAAAAACGCCAGTCAGACCCGGATTACATCGGTTTTCAGGCCTCCGTCATAACCCta > 7:156151/1‑135 (MQ=255) gCCGAGCTGAAACGCAACCTGGCCTAAGCGCCCACGTGGCCGGGCGGG‑TTTTGCAGCGCCCGGCCCGCAGCCCTTTAAAAAAAACCCAGTCAGCCCCCGATTCCATCGGTTTTCAGGCCGCCGTGATCTcccaa > 6:94142/1‑132 (MQ=255) gCCGAGCTGAAACGCAACCTGGCCTAAGCGCCCACGTGGCCGGGCGGC‑TTTTGCAGCGCCCCGCCCGCCCCCATTAAAAAAAACGCCACTCAGACCCGGATTCCAACGTTTTTCAGGCCTCCGTCATCTCCCgata > 2:271231/1‑136 (MQ=255) cAACCTGGCCTAAGCGCCCACGTGGCCCGGCGGG‑GTTTGGCGCGCCCCGCCCGCCCCCCATAAAAAAAACCCCAGTCAGACCCGGCTTGCCACGTTTTTGAAGCCTCCGTCCTCTCCCGATAGTACGCTGAAGGcc > 2:291709/1‑136 (MQ=255) cAACCTGGCCTAAGCGCCCACGTGGCCCGGCCGC‑TTTTGCAGCGCCCGGCCCGCACCCAATAAAAAAAACGCCACTCAGACCCCGATTCGATCGTTTTTCATCCCGCCGTCAAGACCCTATAATCCCCGAAAGGcc > 6:345626/1‑136 (MQ=255) aaCCTGGCCTAAGCGCCCAGGTGGCGGGGCGGC‑TTTTGCAGCGCCCGGCCCGCAGCCATTAAAAAAAACGCCAGTCAGACCCGGATTCCATCGTTTTTCAGGCCTCCGTCATCTCCCTATAGTCCGCTGAAGGCCg < 1:268581/136‑1 (MQ=255) aaCCTGGCCAACGCGCCCAGGGGGCGGGGCGGG‑TTTTGCCGCGCCGGGCCCGCAGCCATTAAAAAAAACGCCAGTCAGACCCGGATTCCATCGTTTTTCAGGCCTCCGTCATCTCCCTATAGTCCGCTGAAGGCCg < 4:332317/136‑1 (MQ=255) ggCCTACGCGCCCCGGGGGCGGGGGGGC‑TTTGCCCCCGCCGGCCCCCCAGCCATAAAAAAAAACGCCAGTCAGACCCGGATTCCATCGTTTTTCAGGCCTCCGTCATCTCCCTATAGTCCGCTGAAGGCCGAtgct < 7:86745/136‑1 (MQ=255) caacccgcCCCCGGGCCCGGGGGGC‑TTTACCCGCCCCCGCCCCGCAGCCATAAAAAAAAACGCCAGTCAGACCCGGATTCCATCGTTTTTCAGGCCTCCGTCATCTCCCTATAGTCCGCTGAAGGCCGATGCTGCa < 4:334229/131‑1 (MQ=255) cTACGCGCCCAGGGGCCGGGGGGGC‑TTTTCCAGCCCCCGGCCCGCAGCCATTAAAAAAAACGCCAGTCAGACCCGGATTCCATCGTTTTTCAGGCCTCCGTCATCTCCCTATAGTCCGCTGAAGGCCGATGCTGCa < 6:144749/136‑1 (MQ=255) cGTGGCCGGGCGGC‑TTTTGCAGCGCCCGGCCCGCAGCCAATAAAAAAAACGCCAGTAAGACCCGGATTCCATCGTTTTTAAGGCCTCCGTCATATCCCTATAGTACGCTGAAGGCCGATGCTGCATAGGTGTaca > 4:244412/1‑135 (MQ=255) gcGGGGGGG‑TTTTGCCGCGCCCGGCCCGCAGCCATTAAAAAAAACGCCAGTCAGACCCGGATTCCATCGTTTTTCAGGCCTCCGTCATCTCCCTATAGTCCGCTGAAGGCCGATGCTGCATCGGTGTACACAACTc < 2:276753/135‑1 (MQ=255) | GGGTTACCTGGCATGGGCCAAGGACTGGCGTGCGCGGGGTGCCAGCATGGTCGGCGGTTGCTGTGGCATCGGCCCGGAGCACATCGCCGAGCTGAAACGCAACCTGGCCTAAGCGCCCACGTGGCCGGGCGGC‑TTTTGCAGCGCCCGGCCCGCAGCCATTAAAAAAAACGCCAGTCAGACCCGGATTCCATCGTTTTTCAGGCCTCCGTCATCTCCCTATAGTCCGCTGAAGGCCGATGCTGCATCGGTGTACACAACTC > ACB143/759280‑759539 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |