| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | ACB143 | 2,390,993 | T→G | 17.6% | E566D (GAA→GAC) | bvgS ← | Virulence sensor protein BvgS |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | ACB143 | 2,390,993 | 0 | T | G | 17.6% | 155.6 / 2.7 | 110 | E566D (GAA→GAC) | bvgS | Virulence sensor protein BvgS |
| Reads supporting (aligned to +/- strand): ref base T (52/34); new base G (17/6); total (70/40) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.31e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 110 total aligned reads displayed.
GGCCTGCACGGCTTCAAGGTAGCTGTAGTTGCAACTTTTCAGGCAACCCTCGCGGTCGCGCACATACATGGGGTGGGGCGTACCGTTGAGCAGGGCGCGCATGAATGCCAACTGGTCGTTCAGCGCGCGTTCGGCGCGCTGGCGCTGGCGAATC‑TGCAGGCGCAGGCGGGCATTCCAGAGCAGGGCCAGCAACAAAAGCACGGCCGTGGCCAGCACCACCTGCAGGGCCAGGCGGCTGTAGCCTTTGGAGTTGTCATCCTCA > ACB143/2390864‑2391125 | ggCCTGCACGGCTTCAAGGTAGCTGTAGTTGCAACTTTTCTGGCAACCCTCGCGGGCGCGCACATACATGGGGGGGGGCGTACCGTTGAGCAGGGCGCGCATGAATGCCAACTGGTCGTTCAGCGCGCGTTCGgcg < 4:13283/136‑1 (MQ=255)ggCCTGCACGGCTTCAAGGTAGCTGTAGTTGCAACTTTTCAGGCAACCCTCGCGGTCGCGCACATACATGGGGTGGGGCGTACCGTTGAGCAGGGCGCGCATTAATGCCAACTGGTCGTTCAGCGCGCGTTCGgcg < 7:16209/136‑1 (MQ=255) gCCTGCACGGCTTCAAGGTAGCTGTAGTTGCAACTTTTCAGGCAACCCTCGCGGTCGCGCACATACATGGGGTGGGGCGTACTGTTGAGCAGGGCGCGCATGAATGCCAACTGGTCGTGAAGCGCGCGGTGGgccc > 5:117004/1‑134 (MQ=255) tGCACGGCTTCAAGGTAGCTGTAGTTGCAACTTTTCAGGCAACCCTCGCGGTCGCGCACATACATGGGGTGGGGCGTACCGTTGAGCAGGGCGCGCATGAATGCCAACTGGTCGTTGAAGGCGCGGTCGGCgcgct > 6:364826/1‑136 (MQ=255) aCGGCTTCAAGGTAGCTGTAGTTGCAACTTTTCAGGCAACCCTCGCGGTCGCGCACATACATGGGGTGGGGCGTACCGTTGAGCAGGGCGCGCATGAATGCCAACTGGTCGTTCAGCGCGCGTTCGGCgcgctggc > 2:101033/1‑136 (MQ=255) tCAAGGTAGCTGTAGTTGCAACTTTTCAGGCAACCCTCGCGGTCGCGCACATACATGGGGTGGGGCGTACCGTTGAGCAGGGCGCGCATGAATGCCAACTGGTCGTTCAGCGCGCGTTCGGCGcgctggcgctgg < 2:204296/135‑1 (MQ=255) cAAGGTAGCTGTAGTTGCAACTTTTCAGGCAACCCTCGCGGTCGCGCACATACATGGGGTGGGGCGTACCGTTGAGCAGGGCGCGCATGAATGCCAACTGGTCGTTCAGCGCGCGTTCGGCGcgctggcgcggggg > 2:15738/1‑134 (MQ=255) cAAGGTAGCTGTAGTTGCAACTTTTCAGGCAACCCTCGCGGTCGCGCACATACATGGGGTGGGGCGTACCGTTGAGCAGGGCGCGCATGAATGCCAACTGGTCGTTCAGCGCGCGTTCGGCGCgctggcgctggc > 8:187726/1‑135 (MQ=255) cAAGGTAGCTGTAGTTGCAACTTTTCAGGCAACCCTCGCGGTCGCGCACATACATGGGGTGGGGCGTACCGTTGAGCAGGGCGCGCATGAATGCCAACTGGTCGTTCAGCGCGCGTTCGGCGCGctggcgctggcg > 6:373865/1‑136 (MQ=255) cAAGGTAGCTGTAGTTGCAACTTTTCAGGCAACCCTCGCGGTCGCGCACATACATGGGGTGGGGCGTACCGTTGAGCAGGGCGCGCATGAATGCCAACTGGTCGTTCAGCGCGCGTTCGGCGCGctggcgcgggcg > 1:45826/1‑136 (MQ=255) cAAGGTAGCTGTAGTTGCAACTTTTCAGGCAACCCTCGCGGTCGCGCACATACATGGGGTGGGGCGTACCGTTGAGCAGGGCGCGCATGAATGCCAACTGGTCGTTCAGCGCCCGTTCGGCGCGctggcgctggcg > 8:193594/1‑136 (MQ=255) cAAGGTAGCTGTAGTTGCAACTTTTCAGGCAACCCTCGCGGTCGCGCACATACATGGGGTGGGGCGTACCGTTGAGCAGGGCGCGCATGAATGCCAACAGGTCGTTAAGCCCGCGTTCGGCGCgcggtcgcgggcc > 3:129870/1‑135 (MQ=255) cAAGGTAGCTGTAGTTGCAACTTTTCAGGCAACCCTCGCGGTCGCGCACATACATGGGGTGGGGCGTACCGTTGAGAAGGGCGCGAATGAATGCCAACTGGTCGTTCATCGCGCGTTCGGCGCGctggcgctggcg > 6:165002/1‑136 (MQ=255) cAAGGTAGCTGTAGTTGCAACTTTTCAGGCAACCCTCGCGGTCGCGCACATACATGGGGGGGGGCGTACCGTTGAGCAGGGCGCGCATGAATGCCAACTGGTCGTTCAGCGCGCGTTCGGCGcgctggcgctgg < 1:24405/134‑1 (MQ=255) gTAGCTGTAGTTGCAACTTTTCCGGCAACCCTCGCGGTCGCGCACATACAGGGGGGGGGGCGTACCGTTGAGCAGGGCGCGCAGGAATGCCAACTGGTCGTTCAGCGCGCGTTCGGCGCGCGGGCGCTGGCGAATc < 4:244604/136‑1 (MQ=255) gTAGTTGCAACTTTTCAGGCAACCCTCGCGGTCGCGCACATACATGGGGTGGGGCGTACCGTTGAGCAGGGCGCGCATGAATGCCAACTGGTCGTTGAGCGCCCGTGGGGCCCGCGGGCGCGGGGGGATC‑Tggcgg > 1:391975/1‑132 (MQ=255) gTAGTTGCAACTTTTCAGGCAACCCTCGCGGTCGCGCACATACATGGGGTGGGGCGTACCGTTGAGCAGGGCGCGCATGAATGCCAACTGGTCGTTGAGCCCGCGGTAGGGGCGCGGGCGGGGGCGAATA‑Tgccgg > 3:358541/1‑136 (MQ=255) gTAGTTGCAACTTTTCAGGCAACCCTCGCGGTCGCGCACATACATGGGGTGGGGCGTACCGTTGAGCAGGGCGCGCATGAATGCCAACTGGTCGTTACGCGCGCGGTCGGCGCGCTGGGGCTGGCGAAAA‑Tgcagg > 7:287594/1‑136 (MQ=255) gTTGCAAGTTTTCAGGCAACCCTCGCGGTGGCGCACATTCTTGGGGTGGGGCGTACCGTTGAGCAGGGCGCGCATGAATGCCAACTGGTCGTTCTGCGCGCGTTGGGCGCGCTGGCGCTGGCGAATC‑Tgcaggcg < 2:1633/135‑1 (MQ=255) cacattttCAGGACACCCTCCAGGTCGCGAAAATACCTGGGGTGGGTGTTAAGGTTCAGCAGGGCGCTCATTAATCCCAACTGGTCGTTCCGCGCGCGTTCGGCGCGCTGGCGCTGGCGAATC‑Tgcaggagcaggc < 8:335992/132‑1 (MQ=255) cAACTTTTCAGGCACCCCTCGCGGTCCCGCACATACATGGGGTGCGGCGGACCGTTGAGCAGGCCGCGCATGAATCCAAACTGGTCGTTCAGCGCGCGGTCGGCGCGGTGGCGCTGGCGAATC‑Tgcaggcgcaggc < 4:157488/136‑1 (MQ=255) cAACTTTTCAGGCAACCCTCGCGGTCGCGCACATACATGGGGTGGGGCGTACCGTTGAGCAGGGCGCGCATGAATGCCAACTGGTCGTTCAGCGCGCGTTCGGCGCGCTGGCGCTGGCGAATC‑Tgcaggcgcaggc < 7:284107/136‑1 (MQ=255) cAACTTTTCAGGCAACCCTCGCGGTCGCGCACATACATGGGGTGGGGCGTACCGTTGAGCAGGGCGCGCATGAATGCCAACTGGTCGTTCAGCGCGCGTTCGGCGCGCTGGCGCTGGCGAATC‑Tgcaggcgcaggc < 5:110909/136‑1 (MQ=255) cAACTTTTCAGGCAACCCTCGCGGTCGCGCACATACATGGGGGGGGGCGTACCGTTGAGCAGGGCGCGCATGAATGCCAACTGGTCGTTCAGCGCGCGTTCGGCGCGCTGGCGCTGGCGAATC‑Tgcaggcgcaggc < 3:357317/136‑1 (MQ=255) aaCTTTTCAGGAAACCCTCGGGGTCGCCCAAATACTGGGGGGGGGGCGTACGGTTGAGCTGGGCGGGCTTGAATCCCAAGTGGGGGTTCTGGGCGGGTTCGGCGCGCGGGCGCTGGCGAATC‑Tgcaggcgcaggc < 4:5148/135‑1 (MQ=255) ttCAGGCAACCCTCGCGGTCGCGCACATACATCGGGTGGGGCGTACCGTTGAGCAGGGCGCGCATGAATGCCAACTGGTCGTTCAGCGCGCGTTCGGCGCGCTGGCGCTGGCGAATC‑TGCAGGCGCAGGCGGGCa < 5:721/135‑1 (MQ=255) cAACCCTCGCGGTCGCGCACATACATGGGGTGGGGCGTACCGTTGAGCAGGGCGCGCATGAATGCCAACTGGTCGTTCAGCGCGCGTTCGGCGCGCTGGCGCTGGCGAATC‑TGCAGGCGCAGGCGGGCATTCCaga < 6:391030/136‑1 (MQ=255) gcgGTCGCGCACATACATGGGGTGGGGCGTACCGTTGAGCAGGGCGCGCATGAATGCCAACTGGGCGTTCAGCGCGCGTTCGGCGCGCTGGCGCTGGCGAATC‑TGCAGGCGCAGGCGGGCATTCCAGAGCAGGGcc < 1:17507/136‑1 (MQ=255) gcgGTCGCGCACATACATGGGGTGGGGCGTACCGTTGAGCAGGGCGCGCAGGAAGGCCAACTGGTGGTTCAGCGCGCGTTCGGCGCGCTGGCGCTGGCGAATC‑TGCAGGCGCAGGCGGGCATTCCAGAGCAGGGcc < 4:128223/136‑1 (MQ=255) acatacttGGGGGGGGGCGTACCGTTGCGCCGGGCGCGCATGAATGCCAACTGGTGGTTCTGCGGGCGTTGGGCGCGCTGGCGCTGGCGAATC‑TGCAGGCGCAGGCGGGCATTCCAGAGCAGGGCCAGCAACaaaa < 3:51346/136‑1 (MQ=255) ctggggggggggCGTACGTTTGCGCTGGGCGCGCATTAATCCCACGTGGTCGTTCCGCGGGGGGGCGGCGCGCGGGCGCTGGCGAATC‑TGCAGGCGCAGGCGGGCATTCCAGAGCAGGGCCAGAAACAAACGCACg < 6:358418/133‑1 (MQ=255) gtggggCGTACCGTTGAGCAGGGCGCGCATGAATGCCAACTGGTCGTTCAGCCCGCGATAGGCGCGCCGGCGCTGGCGAATC‑TGCACGCGCACGCGGGCCTTCCAGAGCCGGGCCAGCAACAAAAGCACGGCCGTg > 6:83088/1‑136 (MQ=255) gtggggCGTACCGTTGAGCAGGGCGCGCATGAATGCCAACTGGTCGTTAAGCGCGCGTTCCGCGCGCTGGCGCTGGCGAATA‑TGAAGGCGCAGGCGGGCATTCCAGAGCAGGGCCCGCCACAAAAGCACGGCCCTg > 1:288252/1‑136 (MQ=255) gtggggCGTACCGTTGAGCAGGGCGCGCATGAATGCCAACTGGTCGTTAAGCGCGCCGTCGGCGCGCTGGCGCGGGCGAATA‑TGCAGGCGCAGGCGGGCATTCCAGAGCACGGCCCCCAAAAAAAGCAccgccgag > 5:66057/1‑134 (MQ=255) gtggggCGTACCGTTGAGCAGGGCGCGCATGAATGCAAACTGGTCGTTAAGCGCGCGTTCGGCGCGCTTGCCCAGCCGAAAA‑TGCAGGACCACGACGGCATTACAGAGAAGTGCCACCAAAAAAAACAAGGACgat > 6:362033/1‑134 (MQ=255) ggggCGTACCGTTGAGCAGGGCGCGCATGAATGCAAACTGGTCGTTCAGCCCGCGTTCGGCGCGCTGGCGATGGCGAAAC‑TGAAGGCGCCGGCGGGCATTACAGAGCAGGGCAAACAAAAAAATCCCGGACGTgga > 2:121507/1‑135 (MQ=255) ggccGTCCCGTGGCGCAGGCCGCGCATCAAGCCAAACGGGGCGTGCCCCGCGGGGGCGGCGCGCGGGCGCTGGCGAATC‑TGCAGGCGCAGGCGGGCATTCCAGACCAGGGCCAGCAACAAAAGCACGGCCGTGGcc < 1:322177/133‑1 (MQ=255) ggCGTACCGTTGAGCAGGGCGCGCATGAATGCCAACTGGTCGTTCAGCGCGCGTTCGGCGCGCTGGCGCTGGCGAATC‑TGCAGGCGCAGGCGGGCCTTACAGAGCAGGGGCAGCCACAAAAGCCCGGCCGTGGccc > 1:52889/1‑135 (MQ=255) ggCGTACCGTTGAGCAGGGCGCGCATGAATGCCAACTGGTCGTTCAGCGCGCGTTCGGCGCGCTGGCGCTGGCGAATC‑TGCAGGCGCAGGCGGGCATTCCAGAGCAGGGCCCGCAACAAAAGCACGGCCGGGGccc > 6:347976/1‑135 (MQ=255) ggCGTACCGTTGAGCAGGGCGCGCATGAATGCCAACTGGTCGTTCAGCGCGCGTTCGGCGCGCTGGCGCTGGCGAATC‑TGCAGGCGCAGGCGGGCATTCCAGAGCAGGGCCAGCAACAAAAGCACGGCCGTGGccc > 3:110790/1‑135 (MQ=255) ggCGTACCGTTGAGCAGGGCGCGCATGAATGCCAACTGGTCGTTCAGCGCGCGTTCGGCGCGCTGGCGCTGGCGAATC‑TGCAGGCGCAGGCGGGCATTCCAGAGCAGGGCCAGCAACAAAAGCACGGCCGTGGCCa > 8:29284/1‑136 (MQ=255) ggCGTACCGTTGAGCAGGGCGCGCATGAATGCCAACTGGTCGTTCAGCGCGCGTTCGGCGCGCTGGCGCTGGCGAATC‑TGCAGGCGCAGGCGGGCATTACAGAGCAGGGCCAGCCACAAAAGCACCGCCGTGGccc > 3:151513/1‑135 (MQ=255) ggCGTACCGTTGAGCAGGGCGCGCATGAATGCCAACTGGTCGTTCAGCGCGCGTTAGGCGCGCTGGCGCTGGCGAATC‑TGCAGGCGCAGGCGGGCATTCCAGAGCAGGGCCCGCAACAAAAGCACGGCCGTGGccc > 5:167214/1‑135 (MQ=255) ggCGTACCGTTGAGCAGGGCGCGCATGAATGCCAACTGGTCGTTCAGCGCGCGGTCGGCGCGCTGGCGCTGGCGAATC‑TGCAGGCGCCGGCGGGCATTGCAGAGCAGGGCCAGCAACAAAAGCCCGGCCGTGGCCa > 6:346315/1‑136 (MQ=255) ggCGTACCGTTGAGCAGGGCGCGCATGAATGCCAACTGGTCGTTCAGCGCGCGGTCGGCGCGCTGGCGCTGGCGAATA‑TGCAGGCGCAGGGGGGCCGTACAGAGCCGGGCCAGCAACAAAAGCACGGCCGTGGccc > 6:5549/1‑135 (MQ=255) cGTACCGTTGCGCAGGGCGCGCATGAATGCCAACTGGTCGTGCAGCGCGCGTTCGGCGCGCTGGCGCTGGCGAATC‑TGCAGGCGCAGGCGGGCATTCCAGAGCAGGGCCAGCAACAAAAGCACGGCCGTGGCCAGc < 7:89711/136‑1 (MQ=255) cGTACCGTTGAGCAGGGCGCGCATGAATGCCAACTGGTCGTTGAGAGCGCGTTCGGCGCGCGGGCGCTGGGGATTA‑TGCAGGCGCATGGGGGCAGTACAAACCATGGGCAGCCAGAAAAGAACGGCCGTTGCCAGc > 4:27018/1‑136 (MQ=255) cGTACCGTTGAGCAGGGCGCGCATGAATGCCAACTGGTCGTTCAGCGCGCGTTCGGCGCGCTGGCGCTGGCGCATC‑TGCAGGCGCAGGCGGGCCTTCCAGAGCAGGGCCAGCAACAAAAGCACCGCCGTGGCCCGc > 3:128162/1‑136 (MQ=255) cGTACCGTTGAGCAGGGCGCGCATGAATGCCAACTGGTCGTTCAGCGCGCGTTCGGCGCGCTGGCGCTGGCGAATC‑TGCAGGCGCAGGCGGGCATTCCAGCGCACGGCCCGCAACAAAATCACCGCCGTGGCCacc > 3:329213/1‑134 (MQ=255) cGTACCGTTGAGCAGGGCGCGCATGAATGCCAACTGGTCGTTCAGCGCGCGTTCGGCGCGCAGGCGCTGGCGAATC‑TTCAGGCGCAGGCGGGCATTCCACAGCAGGGCCAGAAACAAAAGCACCGCCGGGGcccag > 3:239894/1‑133 (MQ=255) cGTACCGTTGAGCAGGGCGCGCATGAATGCCAACTGGTCGTTCAGCCCGCGGTCGGCGCGCGGGCGCTGGCGAATC‑TGCAGGCGCCGGCCGGCATTCCAGAGCCGGGCCAGCAACAAAAGCCCCGCCCTGGcccg > 2:55159/1‑133 (MQ=255) cGTACCGTTGAGCAGGGCGCGCATGAATGCCAACTGGTCGTTCACCGCGCGTTCGGCGCGCTGGCGCTGGCGAATA‑AGCACGCGCAGGCGGGCATTACACAGCAGGGCCAGCAACAAAAGCACCGCCGTGGCCacc > 1:251032/1‑134 (MQ=255) cGTACCGTTGAGCAGGGCGCGCATGAATGCCAACTGGTCGTTCACCGCGCGTTCGGCGCGCTGGCGCGGGCGAATC‑TGCAGGCGCCGGCGGGCATTCCAGAGCAGGGCCAGCAACAAAAGCCCGGCCGTGGCCAGc > 3:125513/1‑136 (MQ=255) cGTACCGTTGAGCAGGGCGCGCATGAATGCCAACTGGTCGTGGAGCGCGCGTTGGGCGCGCGGGCGCTGGGGGCTC‑TGCCGGCGCCGGCGGGGCGTGCACAGCAGGGGCCTCAACAAAACCCAGGCCGTgggccgc > 6:44678/1‑131 (MQ=255) cGTACCGTTGAGCAGGGCGCGCATGAATGCCAACTGGTCGTGGAGAGCCCGTTGGGCGCGCTGGGGCGGGGGAATCTTGCA‑GCGCGCGCGGGGCGTCAACCGCACGGCCAGCCACAAAACCACGGCCGTGGcgcgc > 4:204583/1‑132 (MQ=255) cGTACCGTTGAGCAGGGCGCGCATGAATGACAACTGGTCGTTGAGCGCCCGGGCGGCCCGCGGGCGGGCGGCCCGC‑TGCGCGCGCAGGCGGGCTTGCCACAGCAGGGCCCGCCACAAAAGCACGGCCCTGGccccc > 4:63591/1‑133 (MQ=255) gTACCGTTGAGCAGGGCGCGCATGAATGCCAACTGGTCGTTCAGCGCGCGTTAGGCGCGCGGGCGCTGGCGAATC‑AGCAGGCGCAGGCGGGCATTCCAGAGCAGGGCCACCAACAAAAGCACGGCCGTGGCCCGca > 1:245367/1‑136 (MQ=255) tACCGTTGCGCAGGGCGCGCATGAATGCCAACTGGTCGTTCCGCGCGCGTTGGGCGCGCGGGCGCTGGCGAATC‑TGCAGGCGCAGGCGGGCATTCCAGAGCAGGGCCAGCAACAAAAGCACGGCCGTGGCCAGcac < 7:70455/136‑1 (MQ=255) ggtggAGCAGGGCGCGCATGAATGCCAACTGGTCGTGCAGCGCGCGTGCGGCGCGCTGGCGCTGGCGAATC‑TGCAGGCGCAGGCGGGCATTCCAGAGCAGGGCCAGCAACAAAAGCACGGCCGTGGCCAGcaccac < 5:212802/132‑1 (MQ=255) gTTGCGCAGGGCGCGCTTTAATCCAAACTGGGGGTTCAGCGCGCGTGCGGCGCGCTGGCGCTGGCGAATC‑TGCAGGCGCAGGCGGGCATTCCAGAGCAGGGCCAGCAACAAACGCACGGCCGTGGCCAGCaccacc < 5:315832/136‑1 (MQ=255) gTTGAGCAGGGCGCGCATGAATGCCAACTGGTCGTTCAGCGCGCGTTCGGCGCGCTGGCGCTGGCGAATC‑TGCAGGCGCAGGCGGGCATTCCAGCGCAGGGCCAGCAACAAAAGCCCCGCCGTGGCCAGcacccc > 2:44531/1‑133 (MQ=255) gTTGAGCAGGGCGCGCATGAATGCCAACTGGTCGTTCAGCGCGCGTTCGGCGCGCTGGCGCTGGCGAATC‑TGCAGGCGCAGGCGGGCATTCCAGAGCAGGGCCAGCAACaaaa < 5:258657/113‑1 (MQ=255) gTTGAGCAGGGCGCGCATGAATGCCAACTGGTCGTTCAGCGCGCGGTCGGCGCGCTGGCGCTGGCGAATG‑TGCAGGCGCAGGCGGGGATTCCAGAGCACGGCCCGCAACAAAAGCCCGGCCGTGGCCCGCaccccc > 3:31043/1‑136 (MQ=255) gTTGAGCAGGGCGCGCATGAATGCCAACTGGTCGTTCAGCGCGCGGTCGGCGCGCTGGCGCTGGCGAATC‑TGCAGGCGCAGGCGGGCATTCCAGAGCAGGGCCAGCAACaaaa > 6:258657/1‑113 (MQ=255) aGCAGGGCGCGCATGAATGCCAACTGGTCGTTCAGCGCGCGTTGGGCGCCCTGGCGCTGGCGAATC‑TGCAGGCGCAGGCGGGCATTCCAGAGCAGGGCCAGCAACAAAAGCCCCGCCGTGGCCAGCACCACCTg > 4:385957/1‑134 (MQ=255) aGCAGGGCGCGCATGAATGCCAACTGGTCGTTAAGCGCGCGGTAGGCGCGCGGGCGCGGGCGGAAA‑TGCAGGCGCAGGCGGGCCTTCCAGACCAGGGCCAGCAACAAAAGCACGGCCGTGGCCCCCCCCACCAGCa > 6:147926/1‑136 (MQ=255) aGGGCGCGCATGAATGCCAACTGGTCGTTCAGCGCGCGTTCGGCGCGCTGGCGCTGGCGCATC‑TGCCGGCGCCGGCGGGGATTGCAGACGAGGGCCCGCAACAAAAGCACCGCCCTGGCCAGCCCCACCAGCAggg > 4:53904/1‑136 (MQ=255) aGGGCGCGCATGAATGCCAACTGGTCGTTCAGCGCGCGTTCGGCGCGCTGGCGCTGGCGAATC‑TGCAGGCGCAGGCGGGCATTCCAGAGCAGGGCCAGCAACAAAAGCACGGCCGTGGCCAGCACCACCTGCAggg > 7:289625/1‑136 (MQ=255) aGGGCGCGCATGAATGCCAACTGGTCGTTCAGCGCGCGTTCGGCGCCCTGGCGCTGGCGAATC‑TGCAGGCCCCGGCGGGCATTCCAGAGCACGGCCCGCAACAAAAGCACCGCCGTGGCCAGCACACCCAGCAggg > 2:15338/1‑136 (MQ=255) aGGGCGCGCATGAATGCCAACTGGTCGTTAAGCGCGCGTTCGGCGCGCTGGCGCTGGCGAATC‑TGCAGGCGCAGGCGGGCATTACAGAGCAGGGCCAGCAACAAAAGCACGGCCGTGGCCAGCACCACCTGCAggg > 2:32956/1‑136 (MQ=255) ggCGCGCATGAATGCCAACTGGTCGTTCAGCGCGCGTTCGGCGCGCTGGCGCTGGCGAATC‑TGCAGGCGCAGGCGGGCATTCCAGAGCAGGGCCAGCAACAAAAGCACGGCCGTGGCCAGCCCCACCTGCAGGGc > 7:89778/1‑135 (MQ=255) ggCGCGCATGAATGCCAACTGGTCGTGCAGCGCGCGTGCGGGGCGCTGGCGCTGGCGAATC‑TGCAGGCGCAGGCGGGCATTCCAGAGCAGGGCCAGCAACAAAAGCACGGCCGTGGCCAGCACCACCTGCAGGGcc < 6:73627/136‑1 (MQ=255) ggCGCGCATGAATGCCAACTGGGCGTTCAGCGCGCGGTCGGCGCGCGGGCGCTGGCGAATC‑TGCAGGCGCAGGCGGGCATTCCAGAGCAGGGCCAGCAACAAACGCACGGCCGTGGCCAGCACCACCTGCAGGGcc < 1:317573/136‑1 (MQ=255) ggCGCGCATGAATGCCAACGGGGCGTCCAGCGCGCGTGCGGCGCGCTGGCGCTGGCGAATC‑TGCAGGCGCAGGCGGGCATTCCAGAGCAGGGCCAGCAACAAAAGCACGGCCGTGGCCAGCACCACCTGCAGGGcc < 3:56321/136‑1 (MQ=255) ggCGCGCATGAAGCCCACCGGGCCGTCCCGCGCGCGGCGGGCGCGCTGGCGCTGGCGAATC‑TGCAGGCGCAGGCGGGCATTCCAGAGCAGGGCCAGCAACAAAAGCACGGCCGTGGCCAGCACCACCTGCAGGGcc < 3:242831/136‑1 (MQ=255) ggCGCGCATCACGCCCACGTGGTGGGTCCGCGCGCGGGCGCGGCGCGGGCGCTGGCGAAGC‑TGCAGGCGCAGGCGGGCATTCCTGACCCGGGCCAGCAACAAACGCACGGCCGTGGCCAGCACCACCTGCAGGGcc < 3:98852/136‑1 (MQ=255) gcgcATGAATGCCAACTGGTCGTTCAGCGCGCGTTCGGCGCGCTGGCGCTGGCGAATC‑TGCCGGCGCCGGCGGGCATTCCACAGCAGGGCCAGCAACAAAACCACGGCCGTGGCCCGCCCCCCCGGCCGGGCCacg > 1:109759/1‑134 (MQ=255) gcgcATGAATGCCAACTGGTCGTTCAGCGCGCGTTCGGCGCGCTGGCGCTGGCGAATC‑TGCAGGCGCAGGCGGGCATTCCAGCGCAGGGCCAGCAACAAAAGCACCGCCGTGGCCCGCACCACCTGCAGGGCCCgg > 8:307900/1‑136 (MQ=255) gcgcATGAATGCCAACTGGTCGTTCAGCGCGCGTTCGGCGCGCTGGCGCGGGCGCATC‑TGCAGGCGCCGGCGGGCATTCCAGAGCAGGGCCCGCAACAAAAGCACGGCCCGGGCCCGCCCCCCCAGCCGGGCCCgg > 2:296660/1‑136 (MQ=255) gcgcATGAATGCCAACTGGTCGTTCAGCGCGCGTTCGGCGCGCTGGCGCGGGCGAATC‑TGCAGGCGCAGGCGGGCATTACAGAGCAGGGCCAGCAACAAAAGCCCGGCCGTGGCCAGCCCCCCCAGGCGGGCCAgg > 5:97891/1‑136 (MQ=255) gcgcATGAATGCCAACTGGTCGTTCAGCGCGCGTTCGGCGCGCGGGCGCTGGCGAATC‑TGCAGGCGCAGGCGGGCATTCCAGAGCCGGGCCAGCAACAAAAGCACGGCCGTGGCCCGCCCCCCCTGCAGGGCCAgg > 2:217973/1‑136 (MQ=255) gcgcATGAATGCCAACTGGTCGTTCAGCGCGCGGTGGGCGCGCTGGCGCGGGCGAATA‑TGCAGGCGCCGGCGGGCAGTCCAGAGCCCGGCCAGCAACAAAAGCACCGCCCTGGCCCGCCCCCCCCGcccggcccgg > 4:17189/1‑136 (MQ=255) gcgcATGAATGCCAACTGGTCGTTCAGCGCGCGGTCGGCGCGCTGGCGCGGGCGAATC‑TGCAGGCGCAGGCGGGCATTCCAGAGCAGGGCCAGCAACAAAAGCACGGCCGTGGCCCGCCCCACCAGCCGGGCCCgg > 6:62135/1‑136 (MQ=255) gcgcATGAATGCCAACTGGTCGTTCAACCCGCGGTCGGCGCGCAGGCGCGGGCGAATC‑TTCACGCGCAGGCGGGGATTCCAGAGCAGGGCCAGCAACAAAAGCACGCCCGTGGCCACCACCACCAGCCGGGCCCgg > 4:284327/1‑136 (MQ=255) gcgcATGAATGCCAACTGGTCGTTAAGCGCGCGTTCGGCGCGCAGGCGCTGGCGAATC‑TGCAGGCGCAGGCGGGGATTCCAGCGCAGGGCCAGCAACAAAAGCACGGCCGTGGCCAGCCCCCCCGGCAGGGCCCgg > 2:147380/1‑136 (MQ=255) gcATGAATGCCAACTGGTCGTTCAGCGCGCGTTCGGCGCGCTGGCGCTGGCGAATC‑TGCAGGCGCAGGCGGGCATTACAGAGCAGGGCCCGCAACAAAAGCACGGCCGTGGCCAGCCCCACCTGCAGGGCCAggcg > 5:326236/1‑136 (MQ=255) gcATGAATGCCAACTGGTCGTTCAGCGCGCGTTCGGCGCGCTGGCGCGGGCGAATC‑TGCAGGCGCAGGCGGGCAGTCCAGAGCAGGGCCAGCAACAAAAGCACGGCCGGGGCCCGCCCCACCAGCAGGGCCAggcg > 4:66169/1‑136 (MQ=255) aTGAATGCCAACTGGTCGTTCAGCGCGCGTTCGGCGCGCTGGCGCTGGCGAATC‑TGCAGGCGCAGGCGGGCATTCCAGAGCAGGGCCAGCAACAAAAGCACGGCCGTGGCCAGCACCACCTGCAGGGCCAggcggc < 7:35684/136‑1 (MQ=255) aTGAATCCCAACTGTGCGTCCTGCGCGGGTGCGGGGGGCGGGCGCTGGCGAATC‑TGCAGGCGCAGGCGGGCATACCAGAGCAGGGCCAGCAACAAACGCACGGCCGTGGCCAGCACCACCTGCAGGGCCAggcggc < 6:117004/136‑1 (MQ=255) gAATGCCACCTGGTCGTTCAGCGCGCGTGCGGCGCGCTGGCGCGGGCGAATC‑TGCAGGCGCAGGCGGGCATTCCAGAGCAGGGCCAGAAACAAAAGCACGGCCGTGGCCAGCACCACCTGCAGGGCCAGGCGGCt < 4:366030/135‑1 (MQ=255) aaTGCCAACGGGTCGTTCAGCGCGCGTTCGGCGCGCTGGCGCTGGCGAATC‑TGCAGGCGCAGGCGGGCATTCCAGAGCAGGGCCAGCAACAAAAGCACGGCCGTGGCCAGCACCACCTGCAGGGCCAGGCGGCTGt < 6:215274/136‑1 (MQ=255) ccAACTGGGCGTTCAGCGCGGGTGCGGCGCGCTGGCGCTGGCGAATC‑TGCAGGCGCAGGCGGGCATTCCAGAGCAGGGCCAGAAACAAAAGCACGGCCGTGGCCAGCACCACCTGCAGGGCCAGGCGGCTGTAGc < 2:356571/135‑1 (MQ=255) cTGGTCGTTCAGCGCGCGTTCGGCGCGCTGGCGCTGGCGAATC‑Tgcaggcgcaggc > 6:354094/1‑56 (MQ=255) cTGGTCGTTCAGCGCGCGTTCGGCGCGCTGGCGCTGGCGAATC‑Tgcaggcgcaggc < 5:354094/56‑1 (MQ=255) ggTCGTTCAGCGCGCGTTCGGCGCGCTGGCGCTGGCGAATC‑TGCAGGCGCAGGCGGGCATTCCAGAGCAGGGCCAGCAACAAAAGCACGGCCGTGGCCAGCACCACCTGCAGGGCCAGGCGGCTGTAGCCTTTg > 7:88855/1‑134 (MQ=255) cGTTAAGCGCGCGTTAGGCGCGCTGGCGCTGGCGAATC‑TGCAGGCGCAGGCGGGCATTCCAGAGCCGGGCCCGCAACAAAAGCACCGCCCTGGCCAGCACCACCAGCATGGCCAGGCGGGTGTAGCCGTTGGAGtt > 4:357365/1‑136 (MQ=255) cgggTTGGGCGCGCTGGCGCTGGCGAATC‑TGCAGGCGCAGGCGGGCATTCCAGAGCTGGGCCAGCAACAAAAGCACGGCCGTGGCCAGCACCACCTGCAGGGCCAGGCGGCTGTAGCCTTTGGAGTTGTCATCCTc < 3:93497/133‑1 (MQ=255) cgcgTTCGGCGCGCTGGCGCTGGCGAATC‑TGCAGGCGCAGGCGGGCATTCCAGAGCAGGGCCAGCAACAAAAGCACGGCCGTGGCCAGCACCACCTGCAGGGCCAGGCGGCTGTAGCCTTTGGAGTTGTCATCCTc < 1:279304/136‑1 (MQ=255) gcgTTCGGCGCGCTGGCGCTGGCGAATC‑TGCAGGCGCAGGCGGGCATTCCAGAGCAGGGCCAGCAACAAAAGCACGGCCGTGGCCAGCACCACCTGCAGGGCCAGGCGGCTGTAGCCTTTGGAGTTGTCATCCTCa < 5:347976/136‑1 (MQ=255) gcgTTCGGCGCGCTGGCGCAGGCGAATC‑TGCAGGCGCAGGCGGGCATTCCAGAGCAGGGCCAGCAAAAAAAGCACGGCCGTGGCCAGCCCCACCTGCAGGGCCAGGCGGCGGTAGCCTTTGGAGTTGTCAACcaca > 6:344955/1‑136 (MQ=255) | GGCCTGCACGGCTTCAAGGTAGCTGTAGTTGCAACTTTTCAGGCAACCCTCGCGGTCGCGCACATACATGGGGTGGGGCGTACCGTTGAGCAGGGCGCGCATGAATGCCAACTGGTCGTTCAGCGCGCGTTCGGCGCGCTGGCGCTGGCGAATC‑TGCAGGCGCAGGCGGGCATTCCAGAGCAGGGCCAGCAACAAAAGCACGGCCGTGGCCAGCACCACCTGCAGGGCCAGGCGGCTGTAGCCTTTGGAGTTGTCATCCTCA > ACB143/2390864‑2391125 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |