Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB143 3,803,123 G→A 8.7% R67H (CGC→CAC)  cynR_5 → HTH‑type transcriptional regulator CynR

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1433,803,1230GA 8.7% 141.3 / 4.4 46R67H (CGC→CAC) cynR_5HTH‑type transcriptional regulator CynR
Reads supporting (aligned to +/- strand):  ref base G (30/12);  new base A (2/2);  total (32/14)
Fisher's exact test for biased strand distribution p-value = 5.74e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.15e-01

CGCAGCACCTGCACATCGCCCAGCCCGCGCTGAGCATGGCCGTTACCCGGCTGGAAGAAGAACTGGGGGTAACCCTGTTCAACCGCACCACGCGCAAAGTGATCCTCACGGCAGAAGGCGAGCAGTTCTTGCCGCGCATTGCCTCTGCCCTGCGCGAAATGGACGTTGCCCGGCAAGAGCTGCGCGACATGGCTGACCTCAAGCGCGGTGAAGTACGCCTAGGTATCCCGCCGATGTTCGGCCTGCACTACGTCCCCGGGTTGAT  >  ACB143/3802988‑3803252
                                                                                                                                       |                                                                                                                                 
cgcAGCACCTGCACATCGCCCAGCCCGCGCTGAGCATGGCCGTTACCCGGCTGGAAGAAGAACTGGGGGTAACCCTGTTCAACCGCACCACGCGCAAAGTGATCCTCACGGCAGAAGGCGAGCAGTTCTTGCcgcg                                                                                                                                   <  5:281048/136‑1 (MQ=255)
cgcAGCACCTGCACATCGCCCAGCCCGCGCTGAGCATGGCCGTTACCCGGCTGGAAGAAGAACTGGGGGTAACCCTGTTCAACCGCACCACGCGCAAAGTGATCCTCACGGCAGAAGGCGAGCAGTTCTTGCcgcg                                                                                                                                   <  2:355491/136‑1 (MQ=255)
cgcAGCACCTGCACATCGCCCAGCCCGCGCTGAGCATGGCCGTTACCCGGCTGGAAGAAGAACTGGGGGTAACCCTGTTCAACCGCACCACGCGCAAAGTGATCCTCACGGCAGAAGGCGAGCAGTTCTTGCcgcg                                                                                                                                   <  2:305584/136‑1 (MQ=255)
   agcaCCTGCACATCGCCCAGCCCGCGCTGAGCATGGCCGTTACCCGGCTGGAAGAAGAACTGGGGGTAACCCTGTTCAACCGCACCACGCGCAAAGTGATCCTCACGGCAGAAGGCGAGCAGTTCTTGCCGCGCAt                                                                                                                                >  4:331235/1‑136 (MQ=255)
   agcaCCTGCACATCGCCCAGCCCGCGCTGAGCATGGCCGTTACCCGGCTGGAAGAAGAACTGGGGGTAACCCTGTTCAACCGCACCACGCGCAAAGTGATCCTCACGGCAGAAGGCGAGCAGTTCTTGCCGCACAt                                                                                                                                >  8:101047/1‑136 (MQ=255)
   agcaCCTGCACATCGCCCAGCCCGCGCTGAGCATGGCCGTTACCCGGCTGGAAGAAGAACTGGGGGTAACCCTGTTCAACCGCACCACGCGCAAAGTGATCCTCACGGCAGAAGGCGAGCAGTTCTTGCCGCACAt                                                                                                                                >  8:106541/1‑136 (MQ=255)
     caCCTGCACATCGCCCATCCCGCGCTGAGCATGGCCGGTACCCGGCTGGAAGAAGAACTGGGGGTAACCCTGTTCAACCGCACCACGCGCAAAGTTATCCTCACGGCAGAAGGCGAGCAGTTCTTGCCGCGCATTg                                                                                                                              <  8:255047/136‑1 (MQ=255)
          gCACATCGCCCAGCCCGCGCTGAGCATGGCCGTTACCCGGCTGGAAGAAGAACTGGGGGTAACCCTGTTCAACCGCACCACGCGCAAAGTGATCCTCACGGCAGAAGGCGAGCAGTTCTTGCCGCGCATTGCctct                                                                                                                         >  6:144982/1‑136 (MQ=255)
              aTCGCCCAGCCCGCGCTGAGCATGGCCGTTACCCGGCTGGAAGAAGAACTGGGGGTAACCCTGTTCAACCGCACCACGCGCAAAGTGATCCTCACGGCAGAAGGCGAGCAGTTCTTGCCGCGCATTGCCTCTGccc                                                                                                                     >  3:238412/1‑136 (MQ=255)
               tCGCCCAGCCCGCGCTGAGCATGGCCGTTACCCGGCTGGAAGAAGAACTGGGGGTAACCCTGTTCAACCGCACCACGCGCAAAGTGATCCTCACGGCAGAAGGCGAGCAGTTCTTGCCGCGCATTGCCTCTGCCCt                                                                                                                    <  7:379510/136‑1 (MQ=255)
                  cccAGCCCGCGCTGAGCATGGCCGTTACCCGGCTGGAAGAAGAACTGGGGGTAACCCTGTTCAACCGCACCACGCGCAAAGTGATCCTCACGGCAGAAGGCGAGCAGTTCTTGCCGCGCATTGCCTCTGCCCTgcg                                                                                                                 >  6:45141/1‑136 (MQ=255)
                  cccAGCCCGCGCTGAGCATGGCCGTTACCCGGCTGGAAGAAGAACTGGGGGTAACCCTGTTCAACCGCACCACGCGCAAAGTGATCCTCACGGCAGAAGGCGAGCAGTTCTTGCCGCGCATTGCCTCTGCCCTgcg                                                                                                                 >  4:160506/1‑136 (MQ=255)
                              tGAGCATGGCCGTTACCCGGCTGGAAGAAGAACTGGGGGTAACCCTGTTCAACCGCACCACGCGCAAAGTGATCCTCACGGCAGAAGGCGAGCAGTTCTTGCCGCGCATTGCCTCTGCCCTGCGCGAAATGGACGt                                                                                                     >  3:183351/1‑136 (MQ=255)
                                             cccGGCTGGAAGAAGAACTGGGGGTAACCCTGTTCAACCGCACCCCGCGCAAAGTGATCCTCACGGCAGAAGGCGCGCAGTTCTTGCCGCGCATTGCCTCTGCCCTGCGCGAAATGGACGTTGCCCGGCAAGAGCt                                                                                      >  7:30134/1‑136 (MQ=255)
                                             cccGGCTGGAAGAAGAACTGGGGGTAACCCTGTTCAACCGCACCACGCGCAAAGTGATCCTCACGGCAGAAGGCGAGCAGTTCTTGCCGCGCATTGCCTCTGCCCTGCGCGAAATGGACGTTGCCCGGCAAGAGCt                                                                                      >  2:103680/1‑136 (MQ=255)
                                             cccGGCTGGAAGAAGAACTGGGGGTAACCCTGTTCAACCGCACCACGCGCAAAGTGATCCTCACGGCAGAAGGCGAGCAGTTCTTGCCGCGCATTGCCTCTGCCCTGCGCGAAATGGACGTTGCCCGGCAAGAGCt                                                                                      >  3:223970/1‑136 (MQ=255)
                                             cccGGCTGGAAGAAGAACTGGGGGTAACCCTGTTCAACCGCACCACGCGCAAAGTGATCCTCACGGCAGAAGGCGAGCAGTTCTTGCCGCGCATTGCCTCTGCCCTGCGCGAAATGGACGTTGCCCGGCAAGAGCt                                                                                      <  4:361444/136‑1 (MQ=255)
                                                 gCTGGAAGAAGAACTGGGGGTAACCCTGTTCAACCGCACCACGCGCAAAGTGATCCTCACGGCAGAAGGCGAGCAGTTCTTGCCGCGCATTGCCTCTGCCCTGCGCGAAATGGACGTTGCCCGGCAAGAGCTgcgc                                                                                  <  8:200584/136‑1 (MQ=255)
                                                    ggAAGAAGAACTGGGGGTAACCCTGTTCAACCGCACCACGCGCAAAGTGATCCTCACGGCAGAAGGCGAGCAGTTCTTGCCGCGCATTGCCTCTGCCCTGCGCGAAATGGACGTTGCCCGGCAAGAGCTGCGCGa                                                                                >  6:369130/1‑135 (MQ=255)
                                                    ggAAGAAGAACTGGGGGTAACCCTGTTCAACCGCACCACGCGCAAAGTGATCCTCACGGCAGAAGGCGAGCAGTTCTTGCCGCGCATTGCCTCTGCCCTGCGCGAAATGGACGTTGCCCGGCAAGAGCTGCGCGa                                                                                >  7:92577/1‑135 (MQ=255)
                                                    ggAAGAAGAACTGGGGGTAACCCTGTTCAACCGCACCACGCGCAAAGTGATCCTCACGGCAGAAGGCGAGCAGTTCTTGCCGCGCATTGCCTCTGCCCTGCGCGAAATGGACGTTGCCCGGCAAGAGCTGCGCGa                                                                                >  1:105184/1‑135 (MQ=255)
                                                     gaagaagaaCTGGGGGTAACCCTGTTCAACCGCACCACGCGCAAAGTGATCCTCACGGCAGAAGGCGAGCAGTTCTTGCCGCGCATTGCCTCTGCCCTGCGCGAAATGGACGTTGCCCGGCAAGAGCTGCGCGACa                                                                              >  3:119059/1‑136 (MQ=255)
                                                                    gTAACCCTGTTCAACCGCACCACGCGCAAAGTGATCCTCACGGCAGAAGGCGAGCAGTTCTTGCCGCGCATTGCCTCTGCCCTGCGCGAAATGGACGTTGCCCGGCAAGAGCTGCGCGACATGGCTGACCTCAAgc                                                               <  7:121714/136‑1 (MQ=255)
                                                                         ccTGTTCAACCGCACCACGCGCAAAGTGATCCTCACGGCAGAAGGCGAGCAGTTCTTGCCGCGCATTGCCTCTGCCCTGCGCGAAATGGACGTTGCCCGGCAAGAGCTGCGCGACATGGCTGACCTCAAGCGCGGt                                                          >  5:244170/1‑136 (MQ=255)
                                                                         ccTGTTCAACCGCACCACGCGCAAAGTGATCCTCACGGCAGAAGGCGAGCAGTTCTTGCCGCGCATTGCCTCTGCCCTGCGCGAAATGGACGTTGCCCGGCAAGAGCTGCGCGACATGGCTGACCTCAAGCGCGGt                                                          >  7:331910/1‑136 (MQ=255)
                                                                             ttCAACCGCACCACGCGCAAAGTGATCCTCACGGCAGAAGGCGAGCAGTTCTTGCCGCGCATTGCCTCTGCCCTGCGCGAAATGGACGTTGCCCGGCAAGAGCTGCGCGACATGGCTGACCTCAAGCGCGGTGaa                                                       <  4:41142/135‑1 (MQ=255)
                                                                             ttCAACCGCACCACGCGCAAAGTGATCCTCACGGCAGAAGGCGAGCAGTTCTTGCCGCGCATTGCCTCTGCCCTGCGCGAAATGGACGTTGCCCGGCAAGAGCTGCGCGACATGGCTGACCTCAAGCGCGGTGaa                                                       <  3:84031/135‑1 (MQ=255)
                                                                               cAACCGCACCACGCGCAAAGTGATCCTCACGGCAGAAGGCGAGCAGTTCTTGCCGCGCATTGCCTCTGCCCTGCGCGAAATGGACGTTGCCCGGCAAGAGCTGCGCGACATGGCTGACCTCAAGCGCGGTGAAGt                                                     <  6:30337/135‑1 (MQ=255)
                                                                                aaCCGCACCACGCGCAAAGTGATCCTCACGGCAGAAGGCGAGCAGTTCTTGCCGCGCATTGCCTCTGCCCTTCGCGAAATGGACGTTGCCCGGCAAGAGCTGCGCGACATGGCTGACCTCAAGCGCGGTGAAGTAc                                                   >  7:289930/1‑136 (MQ=255)
                                                                                aaCAGCACAACGCGCAAAGTGATCCTCACGGCAGAAGGCGAGCACTTCTTGCCGCGCATTGCCTCTGCACTGCGCGAAATGTACGTTGCACGGCAAGAGATTCCCGAAATGGCTGACCTCAAGCGCGGTTAAGTAc                                                   >  2:336943/1‑136 (MQ=255)
                                                                                     caccacGCGCAAAGTGATCCTCACGGCAGAAGGCGAGCAGTTCTTGCCGCGCATTGCCTCTGCCCTGCGCGAAATGGACGTTGCCCGGCAAGAGCTGCGCGACATGGCTGACCTCAAGCGCGGTGAAGTACGcc                                                >  2:381536/1‑134 (MQ=255)
                                                                                        cacGCGCAAAGTGCTCCTCACGGCAGCAGGCGAGCAGTTCTTGCCGCGCATTGCCTCTGCCCGGCGCGAAATGGCCGTTGCCCGGCAAGAGCTGCGCGCCGTGGCTGACCTCAAGCGCGGTGACGTACGCCTAGGt                                           >  6:228929/1‑136 (MQ=255)
                                                                                        cacGCGCAAAGTGATCCTCACGGCAGAAGGCGAGCAGTTCTTGCCGCGCATTGCCTCTGCCCTGCGCGAAATGGACGTTGCCCGGCAAGAGCTGCGCGACATGGCTGACCTCAAGCGCGGTGAAGTACGCCTAgg                                            >  3:272558/1‑135 (MQ=255)
                                                                                        cacGCGCAAAGTGATCCTCACGGCAGAAGGCGAGCAGTTCTTGCCGCGCATTGCCTCTGCCCTGCGCGAAATGGACGTTGCCCGGCAAGAGCTGCGCGACATGGCTGACCTCAAGCGCGGTGAAGTACGCCTAgg                                            >  1:36742/1‑135 (MQ=255)
                                                                                          cgcgCAAAGTGATCCTCACGGCAGAAGGCGAGCAGTTCTTGCCGCGCATTGCCTCTGCCCTGCGCGAAATGGACGTTGCCCGGCAAGAGCTGCGCGACATGGCTGACCTCAAGCGCGGTGAAGTACGCCTAGGTAt                                         >  8:164314/1‑136 (MQ=255)
                                                                                          cgcgCAAAGTGATCCTCACGGCAGAAGGCGAGCAGTTCTTGCCGCGCATTGCCTCTGCCCTGCGCGAAATGGACGTTGCCCGGCAAGAGCTGCGCGACATGGCTGACCTCAAGCGCGGTGAAGTACGCCTAGGTAt                                         >  4:181497/1‑136 (MQ=255)
                                                                                             gcAAAGTGATCCTCACGGCAGAAGGCGAGCAGTTCTTGCCGCGCATTGCCTCTGCCCTGCGCGAAATGGACGTTGCCCGGCAAGAGCTGCGCGACATGGCTGACCTCAAGCGCGGTGAAGTACGCCTAGGTATccc                                      >  1:254071/1‑136 (MQ=255)
                                                                                             gcAAAGTGATCCTCACGGCAGAAGGCGAGCAGTTCTTGCCGCGCATTGCCTCTGCCCTGCGCGAAATGGACGTTGCCCGGCAAGAGCTGCGCGACATGGCTGACCTCAAGCGCGGTGAAGTACGCCTAGGTATccc                                      >  8:17852/1‑136 (MQ=255)
                                                                                              cAAAGTGATCCTCACGGCAGAAGGCGAGCAGTTCTTGCCGCGCATTGCCTCTGCCCTGCGCGAAATGGACGTTGCCCGGCAAGAGCTGCGCGACATGGCTGACCTCAAGCGCGGTGAAGTACGCCTAGGTATCccg                                     <  8:279877/136‑1 (MQ=255)
                                                                                                  gTGATCCTCACGGCAGAAGGCGAGCAGTTCTTGCCGCACATTGCCTCTGCCCTGCGCGAAATGGACGTTGCCCGGCAAGAGCTGCGCGACATGGCTGACCTCAAGCGCGGTGAAGTACGCCTAGGTATCCCGCCGa                                 <  7:106541/136‑1 (MQ=255)
                                                                                                  gTGATCCTCACGGCAGAAGGCGAGCAGTTCTTGCCGCACATTGCCTCTGCCCTGCGCGAAATGGACGTTGCCCGGCAAGAGCTGCGCGACATGGCTGACCTCAAGCGCGGTGAAGTACGCCTAGGTATCCCGCCGa                                 <  7:101047/136‑1 (MQ=255)
                                                                                                       ccTCACGGCAGACGGCGAGCAGTTCTTGCCGCGCATGGCCTCTGCCCTGCGCGAAATGGACGTTGCCCGGCAAGAGCTGCGCGACATGGCTGACCTCAAGCGCGGTGAAGTACGCCTAGGTATCCCGCCGATGTTc                            <  6:231997/136‑1 (MQ=255)
                                                                                                        cTCACGGCAGAAGGCGAGCAGTTCTTGCCGCGCATTGCCTCTGCCCTGCGCGAAATGGACGTTGCCCGGCAAGAGCTGCGCGACATGGCTGACCTCAAGCGCGGTGAAGTACGCCTAGGTATCCCGCCGATGTTc                            <  4:358853/135‑1 (MQ=255)
                                                                                                        cTCACGGCAGAAGGCGAGCAGTTCTTGCCGCGCATTGCCTCTGCCCTGCGCGAAATGGACGTTGCCCGGCAAGAGCTGCGCGACATGGCTGACCTCAAGCGCGGTGAAGTACGCCTAGGTATCCCGCCGATGTTCg                           >  3:279878/1‑136 (MQ=255)
                                                                                                              gCAGAAGGCGAGCAGTTCTTGCCGCGCATTGCCTCTGCCCTGCGCGAAATGGACGTTGCCCGGCAAGAGCTGCGCGACATGGCTGACCTCAAGCGCGGTGAAGTACGCCTAGGTATCCCGCCGATGTTCGGCCTg                      >  5:130982/1‑135 (MQ=255)
                                                                                                                aGAAGGCGAGCAGTTCTTGCCGCGCATTGCCTCTGCCCTGCGCGAAATGGACGTTGCCCGGCAAGAGCTGCGCGACATGGCTGACCTCAAGCGCGGTGAAGTACGCCTAGGTATCCCGCCGATGTTCGGCCTGCAc                   >  3:109058/1‑136 (MQ=255)
                                                                                                                           aGTTCTTGCCGCGCATTGCCTCTGCCCTGCGCGAAATGGACGTTGCCCGGCAAGAGCTGCGCGACATGGCTGACCTCAAGCGCGGTGAAGTACGCCTAGGTATCCCGCCGATGTTCGGCCTGCACTACGTCCCCgg        >  5:172442/1‑136 (MQ=255)
                                                                                                                            gTTCTTGCCGCGCATTGCCTCTGCCCTGCGCGAAATGGACGTTGCCCGGCAAGAGCTGCGCGACATGGCTGACCTCAAGCGCGGTGAAGTACGCCTAGGTATCCCGCCGATGTTCGGCCTGCACTACGTCCCCggg       >  5:128104/1‑136 (MQ=255)
                                                                                                                                  gCCGCGCATTGCCTCTGCCCTGCGCGAAATGGACTTTGCCCGGCAATAGCTGCGCGACATGGCTGACCTCAAGCGCGGTGAAGTACGCCTAGGTATCCCGCCGATGTTCGGCCTGCACTACGTCCCCGGGTtgat  <  5:55879/135‑1 (MQ=255)
                                                                                                                                       |                                                                                                                                 
CGCAGCACCTGCACATCGCCCAGCCCGCGCTGAGCATGGCCGTTACCCGGCTGGAAGAAGAACTGGGGGTAACCCTGTTCAACCGCACCACGCGCAAAGTGATCCTCACGGCAGAAGGCGAGCAGTTCTTGCCGCGCATTGCCTCTGCCCTGCGCGAAATGGACGTTGCCCGGCAAGAGCTGCGCGACATGGCTGACCTCAAGCGCGGTGAAGTACGCCTAGGTATCCCGCCGATGTTCGGCCTGCACTACGTCCCCGGGTTGAT  >  ACB143/3802988‑3803252

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑